|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 26 Summary - Ensign's log, stardate 17608.3, Wow what a week! Drying is always pretty simple and been checking daily. Buds are drying nicely and terps are smelling pretty dank!! Most of the week has been in the veg tent with tissue culture! Mixed another batch of establishment media and took proper explant cuttings. Also super-cropped the mothers to even out the canopy. Ahh yea! Getting hyped for trim day next week! Next week will also be the last week for this grow log, but am already planning out the next cycle. Anyways! Onwards to next week! [START OF WEEK 26] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk19] - WEEK 26 - Dry Wk1 ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 09 - Full Veg Gorilla Goat #1 - WEEK 05 - Full Veg Heady OG BX1 - WEEK 09 - Full Veg Garlic Budder OG#1 - WEEK 05 - Full Veg 10/13/25 → 10/19/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~0 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.5 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 00:00 - 00:00 | Wattage: ~000.0W [LV00] Distance From Canopy: ~00” DLI: ~0.00 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.17 kPa Soil %: [47.4, 34.1, 35.0, 55.7, 43.7, 43.6, 44.0, 43.3] ========================================================== Day 01 - Monday - 10/13/25 - Total Days: 183 ----------------------------------- Daily Averages ~[+01:00] - Temps: 78.8°F [26.0°C] - Humidity: 64.4% - VPD: 1.19 kPa - Soil %: [49, 34, 35, 57, 44, 44, 44, 42] ----------------------------------- - [22:05]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great! --- Heady OG BX1 #1 is drying along and no signs of mold. Also airflow and RH is stable and within the 60-70% range. --- Getting stoked! Trim day is 9 days away! - [22:09]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 40% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 40% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [22:18]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- All of the vegging mothers are looking great and the canopy is starting to even out. --- Also clones look healthy and no need to water yet. --- The stems, err, I mean, explants, don’t show any signs of contamination. --- Staying positive, glad the growth media is sterile and the overall procedure was successful. --- Anyways! Plan to create more establishment media and cut proper explants soon! - [22:21]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 10/14/25 - Total Days: 184 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.9°F [24.9°C] - Humidity: 64.8% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 43, 44, 43] ----------------------------------- - [21:44]: Night check on the 4x4 tent. --- Looking good in the dry tent! --- Heady OG is drying along and humidity levels look great! --- Nothing to do but maintain and wait to trim in 8 days! - [21:55]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 41% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 46% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [21:59]: Checked the 3x3 veg tent. --- Looking healthy and growth is raging! --- All mothers are looking beautiful! --- Clones are getting taller and looking healthy! --- Gorilla Goat #2 needs watering. Let’s go Ensign! - [22:03]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 4 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 230.8g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [22:23]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.7g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. --- Decided to train and super-crop both clones. --- Bent the taller branches to lower the canopy. - [22:38]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Onwards Ensign! Day 03 - Wednesday - 10/15/25 - Total Days: 185 ----------------------------------- Daily Averages ~[+01:00] - Temps: 75.6°F [24.2°C] - Humidity: 63.8% - VPD: 1.09 kPa - Soil %: [48, 34, 35, 56, 44, 43, 44, 42] ----------------------------------- - [+01:33]: Quick night check on the 4x4 tent. --- Looking great in the dry tent! --- Also starting to smell dank! --- 7 more days until the big trim! - [+01:37]: Checked the 3x3 veg tent. --- Veg garden is healthy and growth is green! --- Both the Heady OG BX1 #1 and TKO mothers will need another topping soon. --- Clones in the propagation tray also look great and no need to water. --- The explant media is still looking sterile with no signs of contamination. --- Easy night in the garden. Nice! - [+01:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Okay! Tomorrow plan to dive back into tissue culture and prep more growth media. --- Onwards Ensign! Day 04 - Thursday - 10/16/25 - Total Days: 186 ----------------------------------- Daily Averages ~[+01:00] - Temps: 74.6°F [23.7°C] - Humidity: 62.2% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 44, 44, 45] ----------------------------------- - [11:41]: Alright! Back to tissue culture and time to start another batch of establishment media! --- Took the plant growth regulators out of cold-storage (my fridge). ------ [ » SMOKES A BOWL « ] --- While the products warmed up, gathered the necessary supplies and setup my workstations. --- Currently, focusing on: ------ PHASE 01 » ESTABLISHMENT --------- » DAY 01 » MEDIA - PART 01 - Mixing the growth media --------- » DAY 01 » MEDIA - PART 02 - Sterilization of media and tools [via autoclaving] --- Going to use the same establishment formula, but doubling the yield. --- Also using RO water instead of distilled water. --- Here's the establishment media formula: --- [» VER.0001 |» INITIAL TROPICAL PLANTS FORMULA |» 0.5L (500mL) RO WATER «] ------ MS [Muashige & Skoog] --------- » 2.22g ------ Sucrose [Cane Sugar] --------- » 15g ------ BAP [6-Benzlaminopurine | Cytokinin] --------- » 0.5mg » 0.5mL » 500μL ------ IBA [Indole-3-Butyric Acid | Auxin] --------- » 0.05mg » 0.05mL » 50μL ------ AGAR [Jelling Agent] --------- » 3.5g ------ PH [Potential Hydrogen] --------- » 5.7 --- [» 1mg/mL CONCENTRATION ratio | 1mg = 1mL | 1mL = 1000μL «] - [12:59]: Time to prep the growth media! --- Made sure to turn OFF the lung-room's oscillating fan. --- Filled a measuring cup with 400mL of RO water. --- Dropped a clean magnetic mixing bar into the measuring cup. - [13:05]: Placed the measuring cup on the magnetic stirrer. --- Turned the unit ON and have the unit on LOW. - [13:11]: Using a precision scale (sensitive to 0.01g) along with a micro-pipette (1000μL capacity along with tips) and disposable (100mL) weigh boats, ------ Added 2.22g of MS [Muashige & Skoog] ------ Added 15g of Sucrose [Cane Sugar] ------ Added 500μL of BAP [6-Benzlaminopurine | Cytokinin] ------ Added 50μL of IBA [Indole-3-Butyric Acid | Auxin] ------ Added 3.5g of AGAR [Jelling Agent] ------ Then filled the measuring cup to the 500mL line before covering the top with plastic wrap. --- Alright! - [13:46]: Let the media mix on the magnetic stirrer for a bit. --- Have the unit set to LOW with a steady vortex. ------ [ » ~20 MINUTES LATER « ] - [14:09]: Okay! Time to pH the establishment media! --- Gently removed the bottom cap of the AC Infinity PH Meter PRO [AC-PHM7]. Placed the cap in the storage case for later. Covered the top with plastic wrap (KCL solution still inside the cap). --- Swirled the probe in a cup of RO water to rinse off the storage solution. --- Shook the excess water out and wiped the unit with a clean paper towel making sure all mineral buildup was removed (kept away from the very tip of the probe). --- Temporarily placed the meter back into the cup of RO water. --- Removed the plastic wrap of the establishment media. - [14:13]: Using the AC Infinity PH Meter PRO [AC-PHM7], --- Took the establishment media's initial pH reading. --- The pH was at 5.14 --- Using the micro pipette, ------ Added pH Up solution. --- Made sure to let the media solution mix for a bit before checking for pH readings. - [14:21]: Adjusted the media's pH until it stabilized to 5.58 --- For this batch, tried to go for a range of 5.4 to 5.8 pH. --- Also glad that I did not need to add PH down. - [14:43]: Poured the pH'd establishment media into separate polypropylene containers. --- Made sure to re-mix the media solution between container fillings. --- Ended up splitting the media solution into 12 containers. ------ 8x » 125mL Polypropylene Bottles [with screw cap lids] ------ 4x » 236mL (0.5pt) Glass Mason Jars [with polypropylene jamming lids] --- Have the lids of all vessels loosely on and set each aside for later autoclaving. --- Also filled 12 empty polypropylene bottles with RO water and placed the lids on loosely (4 bottles are re-used). - [14:58]: Prepped the tools for sterilization. --- Took a new 5.25in x 10in sterilization pouch and added the following tools: ------ 3x stainless steel forceps ------ 3x stainless steel scissors --- Before placing into the pouch, wiped each tool with a paper towel sprayed with 70% isopropyl alcohol. --- Sealed the bag and marked the date. --- Time to autoclave everything in the pressure cooker! --- Filled a Presto 23qt-Pressure Cooker with 12 cups of RO water. - [15:06]: Added a few aluminum foil balls to the bottom of the pressure cooker. --- Placed the first rack on top of the foil. --- Made sure the rack was level and stable resting above the water. --- Gently placed the containers of media into the pressure cooker and onto the first rack. Lids are still loose. --- Made sure to give space for each container and kept them away from the sides of the pressure cooker. --- Placed a second pressure cooker rack on top of the containers of media. --- Placed the containers of RO water onto the second rack. Lids are still loose. --- Placed the third rack on top of the containers of water. - [15:16]: Lastly, placed the sterilization bag of tools on top of the third rack. --- Took the pressure cooker lid and made sure the release vent was clear of debris before placing it on. --- Sealed and secured the lid. --- Pressure vessel has been sealed! --- Alright! Time to sterilize! --- While keeping the vessel level and straight up, moved the sealed pressure cooker to the kitchen for heating. - [15:23]: Placed the pressure cooker onto a butane-gas stove. --- Turned the stove ON and set it to HIGH. ------ [ » ~20 MINUTES LATER « ] --- Once steam was venting out of the release vent, --- Placed the metal rocker onto the vent. --- Shortly afterwards, the pressure top-lock sprung up to indicate the vessel was under pressure. --- Then the pressure gauge started to rise. ------ [ » ~10 MINUTES LATER « ] --- At 15psi, the metal rocker started to rock! - [15:56]: Turned down the stove down to LOW. --- Set a timer for 15 minutes and began monitoring the pressure gauge. --- Maintained the 15psi by making minor adjustments to the stove. ------ [ » ~15 MINUTES LATER « ] - [16:12]: Turned the stove completely OFF and waited until the pressure cooker's top-lock dropped down which indicated that pressure levels were safe to open. ------ [ » ~40 MINUTES LATER « ] --- Once the pressure cooker was at safe levels (0psi) and the top-lock was down, - [16:48]: Moved the pressure cooker into the lung room. --- The pressure cooker was still quite hot so was careful to keep the vessel straight up and level during transport. --- Used towels for insulation. --- Rested the hot pressure cooker onto a stack of 5-gallon buckets covered with layers of towels and folded paper bags. --- Gently opened the pressure cooker and set the lid aside. - [16:57]: While wearing nylon gloves, sprayed my hands with 70% isopropyl alcohol. --- Gently removed the bag of sterile tools placed it onto a Pyrex dish. --- Removed the top rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle of sterile water before placing onto the Pyrex dish. --- Removed the middle rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle and jar of sterile media before placing onto the Pyrex dish. --- Throughout the entire process, kept the gloves on and sprayed my hands regularly with 70% isopropyl alcohol. --- Decided to store all the bottles in the Pyrex dish. - [17:16]: Have the dish resting on a shelf in the lung room. The jars of media are set right next to the Pyrex dish on a towel. --- To prevent dust, covered everything with towels. --- Done for now and will check on the growth media tomorrow. --- If all goes well, should be taking new explant cuttings in a couple days! - [17:39]: Night check on the 3x3 veg tent. --- Looking beautiful and healthy in the veg garden! --- All moms are getting bushy and getting pretty close to the grow light. --- Since I plan to take explant cuttings soon, not going to top or train quite yet. --- Clones also looking healthy and seem unaffected by the super-cropping. Nice! - [17:57]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 45% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 44% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [17:59]: Checked the 4x4 main tent. --- Ca-Caw!! I got Headies! --- Wow! Heady OG BX1 #1 is drying along beautifully and she is smelling dank! --- Also no signs of mold and RH levels look stable! --- 6 more days until the big trim! - [18:11]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Still gots more to do! --- Let’s go! Let’s grow! --- Onwards Ensign! Day 05 - Friday - 10/17/25 - Total Days: 187 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.0°F [24.4°C] - Humidity: 62.0% - VPD: 1.16 kPa - Soil %: [48, 34, 35, 55, 44, 44, 44, 42] ----------------------------------- - [22:57]: Night check on the 4x4 tent. --- Ca-Caw! Headies is smelling dank! --- RH levels are getting a bit dry, but are okay. --- Wow buds are frosty with trichomes! --- With 5 days until trim day, getting stoked! - [23:04]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 47% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:11]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- Mothers are getting bushy and tall! --- Super-cropped and trained the taller branches down to lower the canopy. --- Plan to take tissue culture cuttings tomorrow so glad there are plenty of tops to choose. Still deciding on which mother to preserve next. --- Clones in the propagation tray look healthy! --- The original Garlic Budder #2 clone needs watering. - [23:26]: Here is the starting initial watering range: ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Garlic Budder OG#2 » 258.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:40]: Took the saturated weight after watering, ------ Garlic Budder OG#2 » [000.0]g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [23:45]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 10/18/25 - Total Days: 188 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.8°F [24.9°C] - Humidity: 60.3% - VPD: 1.25 kPa - Soil %: [47, 34, 35, 55, 44, 44, 44, 47] ----------------------------------- - [15:19]: Decided to decrease the VPD level a bit. --- Within the AC Infinity App, --- Changed the schedule name from 'Flower [12/12]' to 'Drying [0/24].' Oops. --- For the Main Tent [4x4] controller, --- Adjusted the 'Drying [0/24]' Schedule for the exhaust fan. ------ Decreased the VPD LOW trigger value from 1.5kPa to 1.4kPa --- Adjusted the 'Drying [0/24]' Schedule for the humidifier. ------ Decreased the VPD target value from 1.5kPa to 1.4kPa --- This should increase the relative humidity a bit and will maintain a 60-65% range - [16:59]: Evening check on the 3x3 veg tent. --- Wow the veg garden is raging with healthy growth! --- Even with the lower veg temps, growth has been perky and bushy! --- The TKO mother has been looking a bit on the purple-side, but I plan to increase the temps back after trim day. - [17:23]: Okay! Time to continue the tissue culture journey! --- Checked on the sterilized media, water, and tools. --- Ooo yea! The growth media in the glass jars have solidified nicely! --- The media in the bottles also look great! Found only one that did not gel properly. --- Hmm. The sterile water bottles are not looking sterile. Unlike the first batch (using distilled water), each RO bottle had tiny white particles floating in the water. --- I think this was due to the water source. The RO water used was from the plant reservoir. For the next sterilization, will try using water straight from the tap. Will discard the entire batch and try again later. - [17:34]: Anyways! Time to cut and sterilize another batch of explant cuttings. --- Using the similar protocols as last time, but with a few changes. --- Instead of distilled, using reverse-osmosis (RO) water. --- Also simplifying the workflow and will not be using the sterile water. --- Only taking cuttings from one cultivar. --- Today, attempting to preserve Heady OG BX1 #1 --- Unlike the last attempt, going to be cutting apical nodes and will leave all leaf growth on. --- Last attempt was more for monsteras and not cannabis. - [17:35]: Made sure the lung room's oscillating fan was OFF during the entire process. --- Put on a fresh pair of nitrile gloves. --- Okay! Let's grow Ensign! - [17:47]: Filled a glass beaker with 180mL of RO water. --- Using a micro-pipette, ------ Added 20mL of Clorox bleach --- Mixed the solution. - [17:52]: Poured the bleach solution into a glass mason jar. ------ Added 1 drop of Dawn dish soap. --- Dropped a magnetic mixing rod into the jar before setting it onto the magnetic stirrer. --- Turned the unit ON and set it to a low setting. --- Placed a polypropylene lid loosely on. - [18:02]: Rinsed and filled the glass beaker with 70% isopropyl alcohol. --- Filled another glass beaker with RO water before adding a drop of Dawn dish soap. Mixed the solution. --- Okay! Time to take the cuttings! --- Using a clean pair of trimmers, - [18:09]: Gently cut two clone cuttings from the Heady OG BX1 #1 mother. --- Stripped each cutting down to 2-inch apical nodes making sure to leave about 0.5 inch of stem growth for planting. ------ Prepared a total of 2 nodal cuttings. --- Dropped the nodal cuttings into the cup of soapy water. --- Swirled the cuttings in the soapy water for a bit. --- Opened the jar containing the 10% bleach solution. --- Using clean tweezers, --- Took each cutting out of the RO water and carefully submerged it into the beaker of 70% isopropyl alcohol for about 30 seconds. --- Then transferred the cutting from the isopropyl alcohol straight into the jar of bleach solution. --- Once both cuttings were in the jar, sealed the top with the polypropylene lid. - [18:23]: Flipped and set the entire bleach jar upside down onto the magnetic stirrer. --- Started using the magnetic stirrer. --- But the cuttings were getting hit by the bar so ended up turning the unit OFF. --- Instead, made sure to swirled the bottle every few minutes to agitate the cuttings. --- For a proper sterilization, the cuttings should be agitated in the hypochlorite (bleach) solution for at least 20 minutes. --- While waiting for the explants to sterilize, prepped the still air box. - [18:39]: Unfolded and placed a Safloga still-air box onto my desk. --- Thoroughly misted the inside of the air box with 70% isopropyl alcohol making sure to coat the entire chamber. --- Then isopropyl misted and placed the following items inside of the still air box, ------ x1 » Jar of Establishment Media ------ x1 » Bottle of Establishment Media ------ x1 » Glass-Bead Sterilizer ------ x1 » Roll of Plastic Wrap [pre-cut] ------ x1 » Sterilization Bag Containing: --------- x3 » Stainless Steel Forceps --------- x3 » Stainless Steel Scissors --- Made sure to fully coat the outside of the tool bag and each of the containers with 70% alcohol. --- The bag had a torn corner (Oops), so made sure to spray the inside with a few mists of isopropyl alcohol. --- For the glass-bead sterilizer, ------ Wiped the entire unit with isopropyl alcohol. ------ Before filling the unit with the included beads, misted the heating chamber with a spray of isopropyl alcohol. ------ Misted the glass beads again with a spray of isopropyl alcohol. --- Set the cleaned glass-bead sterilizer into the still air box. - [19:02]: Still air box fully prepped for explants! --- Okay! Explant nodal sterilization complete! --- While still wearing gloves, --- Misted the outside of the explant jar with 70% isopropyl alcohol before placing it into the still-air box. --- Turned the glass-bead sterilizer ON. --- Made sure to re-seal the chamber. - [19:05]: Okay! Finally time to start the tissue culture transplant! --- Let's go Ensign! --- Sprayed my gloved hands with 70% isopropyl alcohol (also put on a pair of cloth arm sleeves), --- Placed my hands through the arm holes and into the still air box to begin the process. --- Started by laying out my initial workspace: ------ » Far Left Quadrant » Jar of Sterile Establishment Media ------ » Far Center » Bottle of Sterile Establishment Media ------ » Far Right Quadrant » Glass-Bead Sterilizer ------ » Mid Center » Bleach Jar with Explant Cuttings [Main Workspace] ------ » Near Left Quadrant » Empty [Trash Area] ------ » Near Right Quadrant » Bag of Sterile Tools & Roll of Plastic Wrap --- Opened the bag of tools and placed the forceps and scissors into the pre-heated glass-bead sterilizer. --- Set the bag in the trash area (near left quadrant). --- Loosened the lids of both containers of sterile media but did not remove the lids. The lids were resting on top and could easily be lifted. --- Also took lid off of the bleach jar and gently set it on the table. --- Took the jar of sterile media and set it in the center of my workspace (to the right of the bleach jar). --- Using my left hand, took a set of scissors from the glass-bead sterilizer and gently cut the bottom of the first cutting. --- Once the bottom cut was made, placed the scissors back into the glass-bead sterilizer. --- Using my left hand, lifted the lid off of the jar of sterile media and inserted the trimmed end of the cutting straight into the growth media (using my right hand). --- Covered the jar with the lid and placed the forceps back into the glass-bead sterilizer. --- Tighten the lid of the jar and set it aside. ------ [ » REPEATED THE ABOVE PROCESS FOR THE BOTTLE OF GROWTH MEDIA « ] --- Once the last cutting was transferred from the bleach jar into the sterile media, --- Turned the glass-bead sterilizer OFF. --- Cleaned up my workplace and placed the scrap stem tips and bleach jar out of the way (near left quadrant). - [19:28]: Wrapped the lid of each explant container with plastic wrap before setting it in the center of the workspace. --- Explant transplant completed! - [19:59]: Using a label printer (Brother DT410), made a set of labels for each container: ------ Heady OG BX1 #1 | 04.24.25 | Photo | Reg ------ Formula: v0001 | ExBatch: 0002 | Lot: 01 | Pod: 01 ------ Establishment Media | Sealed On: 10/18/25 --- Opened the still air box. --- Then one container at a time, took an explant out and wrapped the lid with a label. --- Set the labeled containers onto the propagation tray (in the 3x3 veg tent). - [20:07]: Done! ------ The explants are around 37 μmol/m²/s which gives a DLI of 2.40 mol/m²/d under an 18/6 light cycle. --- Will check daily for contamination. --- Ca-Caw!! Ahh yea! --- Heady OG BX1 is now officially my first true attempt at cannabis tissue culture! --- Using this new workflow, I may try reusing the TKO media. Do plan to take new cuttings soon. May even try using the liquid media. --- Shout out to microclone and Bill Graham! His 'Tissueponics' video contains tips and tricks of the trade that have straight up changed the way I think of DIY cannabis tissue culture. --- Plan to further refine my process and will continue to refine the workflow with each attempt. - [20:15]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 54% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 48% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 45% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:56]: Checked the 4x4 tent. --- Ca-Caw! Looking great my ladies! --- Humidity levels are still a bit on the dry side, but still okay. - [21:08]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Oh yea, 4-more days until trim day!! Ca-Caw! Let’s go! Day 07 - Sunday - 10/19/25 - Total Days: 189 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.7°F [24.8°C] - Humidity: 59.4% - VPD: 1.27 kPa - Soil %: [48, 35, 35, 55, 44, 43, 44, 42] ----------------------------------- - [07:08]: Quick morning checked on the 3x3 veg tent. --- The veg garden is healthy and perky! --- All moms and clones are healthy and raging with growth! --- Both Heady OG BX1 #1 explants also look alive and green! - [19:26]: Night check on the 4x4 tent. --- Ca-Caw! We got MENDO DOPE in the trunk! --- Wow terps are getting loud and buds are drying along nicely! --- Looking frosty and coated in trichomes! --- Three more days until trim day!! - [19:33]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 46% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 43% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [19:35]: Checked the 3x3 veg tent. --- Vegging along beautifully in the garden! --- Mothers are getting taller and probably could train, but will wait until tomorrow. --- Clones and explants also look healthy and green! --- Gorilla Goat #2 needs watering! - [19:44]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 5 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 244.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [20:04]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.6g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [21:41]: With a few days before the big trim, decided to sample a few buds! --- Cut and trimmed branch for sampling and wow she’s got terps! --- Buds are dense and have a gassy diesel funk! Ca-Caw!! Wow! --- Without even curing, pretty sure she’s the one! --- Plan to grow out another cycle (in clone of the same pheno), but I think I found my female for my first breeding project! - [22:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Ahh yea! Three more days until the big trim! --- Also next week will be the last for this log. --- But already stoked for the next! --- Planning on popping more seeds and continuing the pheno hunt for another female! --- Since I lost TKO this harvest cycle, plan to grow a clone cutting of the same pheno (#2). --- As for seeds, popping more regulars and going to attempt to grow some of Mr. Grow-It’s Chillout OG! --- Got a pack of F3s and am stoked to give them a try! --- Also going to sprout some beans of Dead Ops OG from Zaza Genetics! --- Chillout OG, Dead Ops OG, and another round of TKO! Let’s go! Let’s grow! --- Hunters of the dank! Ca-Caw! --- Anyways! --- Onwards to next week! [END OF WEEK 26]

9/14 Week 9 and they are doing fine Little vid Hero Left rear Bea rt rear They are almost identical now Bea a bit larger Nuets not changing for now 9/16 Doing well but some white tips are saying we gotta reduce the salts so will be dropping CT nuets 10% and upping PK to 2.5 Extra space is really doing them well Defoliation again tonight the war on stemmed leaves has begun 9/17 Held a meeting of the minds and decided the best course of action is to flip the light schedule so I can feed them more appropriately, twice a day. Leaving nuets as they are as this will drop pot EC. Setting the alarm for the AM before lights out at 7:30 and changing the sched to 6AM-6PM summer is done no need for current schedule. Lights be out from 7:30AM to 6AM long I know but should not bother them. Made a bouquet of all the sucker branches, seems to be a lot on FF11 9/19 Lights on girls feed buds look fine Video enjoy

Week 9 coming along and she is very thirsty always want more nutrients this week I feed 3ml alg amic 5ml acti vera 5ml heaven 5ml bloom 5ml top max 1ml atami bloombastic And feed her 2L every morning 🌄 I think 2 weeks left to harvest coz she start flowering day 21 will see

Starting to look really nice had to take it out the tent as I hate the yellow tinge from the bulb cannot see the correct colour of leaves or how frosty she looks leaves look a little dark gonna start flush next watering , giving just over 2l every seccond day if I was to do this again I’d use a bigger pot but I was expecting this to be an auto. Branch with fasciation split into 4 still gonna get some nice buds on it just not as much as I could of had I noticed earlier in the grow

So this lady was the biggest of all and the fastest. The smell is very nice, sweet and lemon. Was very easy to grow, all natural, only good soil and water.

OK guys so i figured i would do a video update instead of pictures now that we have some flowers! They are gaining weight everyday now that i got things under control. I went through a pH upswing again and still cannot find the cause of it. but regardless we are back on track i believe

Ladies and gentlemen we are on day 82 and this girl is no more, through the loophole I can't find one clear trichome I understand the white hairs but I also understand the trichomes is what we go by.. also wanted to get her down because I was worried of possible root issues and did not want to ruin such a beautiful little plant.. Divine seeds has some great genetics this thing was topped and in such a small cup handled the training well and rewarded me with what I'm assuming is going to be between 28 and 35 grams so I'm thinking over a gram per ounce of dirt that's not too shabby.. I hope everyone in the contest is doing well and I hope everyone else is doing just as good.. we are all in this together, to be able to grow our own medicine at our own discretion and know what we're smoking is worth its weight in gold.. again big shout out to Divine Seeds for putting together this competition I thought I would do something a little different to stand out by doing a mini pot grow I don't know if it'll get me a trophy or not but it was sure fun growing it and was very beneficial to me in my personal development as a grower.. until next time folks and a month or so I will have the smoke report.. I tried to upload today for another strain and it wasn't working so hopefully grow diaries fixes the bug of not being able to update a smoke report.. happy growing everyone ✌️😎 ladies and gentlemen after 8 days of drying I trimmed her up today and as you can see from the video 46 g or 1.625 Oz which is extremely good considering the small amount of medium... Now I know during the cure I will probably lose around 1/8 of my total weight but still I will be definitely over 40 grams my guess will be at least 42 to 43 .. now for 28 Oz of soil I could not be happier.. between the genetics being great and the plants loving my feeding remedy all has been well.. I want to thank Divine seeds for throwing such an awesome contest and giving us enough time to do it right and also for providing the seeds but not just that they really hooked it up.. Big ups to them and I have more genetics that I will be running from them in the future.. hope everyone is doing well good luck to everyone in the contest and to everyone happy growing ✌️😎

Day 15 since seed touched soil. All is going amazing. Yesterday topped them up a bit. Stems looks lanky, would expect stronger by now, but generally girls fly ! Off course i watered them straight after goodies were rubbed into top soil. Great White - 4 g sprinkled around for each plant BioSys same amount. Ecothrive Bio Blend - 200 grams added to each pot. Devil biggest, nicest and is happy camper at the moment. Lights are still on 40%, Mars as always has great products for growers. Think this week i will start some training. Day 19. Yesterday girl got 800 ml of 6.3 ph water. She is flying 4sure, thinking to train her tomorrow. Day 20. First LST. Watering straight after, plus Biobizz Leaf Coat shower. Approx 2.5 liters went in, first big saturation. Happy Growing !!!

Nach langer Zeit ist es endlich soweit, ich habe sie geerntet. Bei dieser Pflanze habe ich mich dafür entschieden sie gegen den natürlichen Lebenszyklus outdoor eher zum Blühen zu bringen. Ich pflanzte sie in einen Hanffasersack den ich mit Erde auffüllte. Diesen platzierte ich auf einer Schubkarre. Wo ich wohne wird es manchmal schon im Oktober sehr kalt, sodass die Wahrscheinlichkeit für eine sichere Ernte realtiv gering ist (at this point i hope the best for my other outdoor plants). Ich rechnete mir aus wann der perfekte Zeitpunkt zur Ernte ist um noch im Sommer das Resultat genießen zu können. Ab diesem Zeitpunkt fuhr ich die Schubkarre jeden morgen um 8 Uhr nach draußen und um 18 Uhr wieder nach drinnen um optimale 12 Stunden Lichtzufuhr zu gewährleisten. Während den letzten Wochen der Blüte sind ein paar männliche Blüten aufgetaucht wie auch bei meinem indoor grow. Für einige war der Zeitpunkt der Ernte vielleicht zu früh, aber ich finde es so sehr angenehm zu rauchen und auch der Effekt ist in diesem Stadium optimal für mich. Die einzige Methode die ich hier verwendete ist das Pruning. Es erspart dir einen Menge Zeit beim beschneiden der Blüten und lenkt die Kraft auf die verbleibenden Blüten, um diese zu stärken. Nachdem ich bei meinem letzten outdoor grow viel zeit beim trimming verbracht habe, habe ich mir eine Erntemaschine gekauft (look at the video). Mit dem Ergebnis der Maschine bin ich zufrieden, da ich es per Hand in der gleichen Zeit unmöglich in der Qualität geschafft hätte. Die Blüten wurden ungefähr 2 Wochen bei guter Durchlüftung getrocknet, um am Ende wurden es 255g. Für mich persöhnlich ist es ein sehr gutes Ergebnis. Die Blüten sind schön fest und klebrig mit einem sehr süßlichen Geruch. Ich habe versucht ein paar schöne Fotos zu machen und habe meine Kreativität spielen lassen (my wife also got a great idea, you can guess which idea was mine ;D) If you have any questions feel free to ask. Im also open for criticism I hope you enjoy the pictures and the report stay safe and happy growing. ____ i will update the smoke report soon.

Hallo zusammen 🤙. Sie wächst sehr schön und macht keine Probleme. Rabattcode für den BIOTABS-Webshop https://biotabs.nl/en/shop/ GDBT420, damit erhalten Sie 15 Prozent

A wonderful genetic thank you Fast Buds ❤️‍🔥🤩 The trim was super easy the buds are FAT & FROSTY full of resin 🤤 I’m very happy with the harvest 96g !! For the light i’ve used the Spider Farmer SE-7000💡

Looking like 3 more weeks until harvest. I’m going to start flushing in about 10-12 days

ROYAL QUEEN CBG AUTO ROYAL QUEEN SEEDS Week #6 Ovetall Week #1 Flower This week she's looking good even after I shocked her I have then cold water being in a hurry that being said she's doing great starting to make some nice looking buds and she looks like a robust plant. Stay Growing!!

Had a good week. Started flowering. Julie and Jen had some lime green new growth but i upped the nutes and today they look much better. First pistils showed up on Julie and her canopy is coming along.

Day 141 still going started to reveg but lowered light schedule

Just checking the trichome heads daily to harvest them at the for me perfect moment.

Ladies, gentlemen, and all the wonderful beings following this journey, I am thrilled to announce that we have reached the highly anticipated harvest week for our beautiful PCRs. This has been an incredible journey, and now it's time to reap the rewards of our hard work and dedication. The PCRs are nothing short of perfection. Their frost-covered buds are a sight to behold, glistening like they've been kissed by winter's breath. The trichomes are abundant and sparkling, making these ladies look absolutely stunning. The aroma is intoxicating, a true testament to their strong genetics and the care they've received. Upon taking them out of the tent, I was amazed by their strength and resilience. Despite my best efforts to make them dance, these ladies stood tall and proud, requiring no support at all. This just goes to show the exceptional quality and sturdiness of the PCR strain. Aptus Holland's Enzym+. This product is a game-changer, breaking down dead root material and turning it into usable nutrients, ensuring our soil remains rich and fertile for future grows. Speaking of which, I do plan to reuse the soil, thanks to the amazing benefits of Enzym+. This enzyme product not only helps with nutrient uptake but also keeps the soil ecosystem healthy and thriving. Here's the final stats for this week: TDS: 130 PH: 6.8 Temp: 21°C A huge shout-out to Aptus Holland for their incredible products that have consistently supported my plants' growth and health. Also, a massive thank you to Art Genetix for creating the phenomenal PCR strain that has brought so much joy to this grow. To all my fellow growers, followers, and the entire community, your support and encouragement mean the world to me. This journey wouldn't be the same without you. As we move forward, let's continue to spread love, positivity, and the joy of cultivating these amazing plants. Stay tuned for the final results and more updates as we dry and cure these beautiful buds. Until then, keep growing, stay positive, and never stop believing in the magic of cultivation. Peace, love, and happy growing! Genetics - P.C.R. @Art_Genetix_Team https://artgenetix.world/ Nutricion @aptusholland https://aptus-holland.com/ LED Power @Lumatek and @viparspectra As always thank you all for stopping by , for the love and for it all, i fell blessed to have you all with me for one more love journey Thank you Thank you Thank you , you guys are great and have been amazing , thank you for everything ! #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #dogdoctorofficial #growerslove
 With true love comes happiness , Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so ! Friendly reminder all you see here is pure research and for educational purposes only Growers Love To you All Discount Codes : @thecannakan DOGDOCTOR 15% off @theneutralizer PORKIT5-DOG 15% off @kannabiaglobal DOGDOCTOR 30% off @seedsmangenetics DOGDOCTOR 10%off @terpyz.mutant.genetics DOCTOR 15% off Have a fantastic week!

Forbidden runtz auto.. è partita con una delle prime foglioline storpia...ma a vederla sembra stia venendo bene ..vedremo più avanti.,....... II

In this week I pulled out a few males, see video with male parts. All plants are seeing an explosion of growth and have all recieved first topping with some plants already being topped for the second time at the point in the grow. All plants receive teas at every feeding never feeding the plants only feeding the soil

Nutes at 1/2 strength..picked some supplements to aid in taste/flowering so excited to see how she finishes! 😋😋