Pineapple Expressx2 (PE), Auto Durban Poisonx3 (ADP): Day 77 from seed. PEx2 Week 3 Flower & ADPx3 Week 5-6 Flower. Environment has been crazy fluctuations from outside weather: Temps 73-90F, RH 45-68%, CO2 500-875, & PPFD 1150-1250. Received a Compost tea to run off this week. All plants looking green and healthy. Flowering nicely. ADP #2, the most mature of the plants, Trichomes are coming along. Over all 55% Milky, 35% clear, 10% Amber. Going to give it another week of ripening and check again. The fade not real strong yet as well. 👌

Day 22. She took the little LST very well. Gave her a full flush today with simple ph6.0 water. When the pot dries up I'll start feeding her with some more nutrients. Day 26.... Fixed the pH issue this week. She's doing well but I think next week I'll give her a little more nutrients to see how she reacts. She's doing well on day 27. She responds very well with the tucking of her big leaves. I will definitely fill my 3 gallon pots a lot more on my next grow because it's very difficult to water or even lst these plants. There's a lot of side shoots on this plant which I'm happy about. I'm happy with her growth. As you can tell from my other grow this one is doing way better.

Week 3 for the solo is officially complete. She's been growing with no trouble. Root development has been excellent. As you can see, I decided to mainline. Seeing as she's in a solo cup, I'm not too worried about stunting. I'm horrible at measuring the water intake, but the cup needs to be watered daily. It's as lite as a feather everyday.

Don't have a lot of time these days to take pics so I'll sum things up. CO2 got outta control and the plants took tf off literally. The video is around middle of week 3, I had to flip at week 4 BC I literally ran out of room and I had to constantly defoliate.

Day 35 and she is a beauty. She has a nice color and I love her structure. I am going to call it flower this week since she is well into preflower. The system is running great. Haven't had any roots try to grow into the pipe yet. Going to start them on a little maxibloom soon.

Can’t wait 🔥🌬️💨💨💨🍃🍃🍃🍃🍃🍃🍃🍃🍃

Hey ! I was away from home this week, so i only have pictures of the Day 20 of 12/12. Have a deficience of N. Added some biogrow ans started the pk booster. See ya next week ✌️

Gracias al equipo de AnesiaSeeds, Marshydro, XpertNutrients y Trolmaster sin ellos esto no sería posible. 💐🍁 Oracle Octane: Oracle Octane es una cepa altamente potente y con un alto contenido de THC elaborada a partir de una Blackberry Octane seleccionada, excepcionalmente rica en THC y aromática, y de la pesada Blackberry Oracle, inédita, de nuestro propio acervo genético. El desarrollo de esta carismática e impresionante variedad abarcó 8 generaciones y un enorme esfuerzo de cría. La variedad tiene un sabor intenso e inolvidable que recuerda a bayas dulces y limones frescos. Las plantas a partir de semillas crecen increíblemente hermosas y sus hojas y cogollos a menudo desarrollan tonos de rojo, morado intenso e incluso casi negro. Esta cepa vigorosa y ligeramente índica dominante tiene un tiempo de floración de unas 9 semanas, produce hojas grandes y alcanza una altura de unos 120-130 cm en el interior. 📆 Semana 1: Ha sido una buena semana, ella ha dado otro gran cambio en su lugar definitivo 😎. La carpa está ocupada al 75% y comienza una floracion explosiva gracias a @Marshydro y @Xpertnutrients y @Trolmaster con esta gran genética 💪. A partir de ahora se riega manualmente con las dosis recomendadas por el fabricante.

This babe is a frost monster! Smells like permanent marker, curious where that came from? Structure very much matched description, but the smells. It's insane how burnt rubber it smells! Chopping today, day 101.

El olor es cada vez más dulce y afrutado, 3 semanas y estarán listas para la cosecha.

Packing on weight and has this og earthy smell . Getting sticky and white

5 week of flowers ...)) I defoliated the girls....work in progress....💚 I see so many buds I hope everything will proceed for the best because the girls have just stretched too much .... also made some emergency folding to those who had come very close to the lamp ....

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Pollination is well underway. The ladies are prego and the seeds are developing very rapidly! A few of the first flowers to get hit have there flower bracts already torn open. Exposing the growing seeds(still very green and immature). They are coating there flowers with protective trichomes like crazy! Sucess. All three ladies I have in there are heavily seeded! Now I'm watching them work their magic. Producing me some new killer crosses to phenohunt. The male flowers have been dried and stored. I'm slowly sifting the pollen into a vile. Whenever I get a chance to play with pollen lmfao. I've only made a dent in my freezer bag supply. Until next update. Happy growing and stay lit fam.

Yo, I aim to impress with this grow! I’ll be giving it my all. The nutes I’ll be using will be organic Gaia green 4-4-4, they’re guano 0-9-0, and can’t forget the bloom 2-8-4. This girl will be a beast. These seeds were given to me from @Premium_Cultivars for the competition they are running here on GrowDiaries! I wish everyone the best of luck and may the best growers win! (We are all winners, we already be growing some of this fire 🔥) Started off with 48hrs in glass of water then went to 24+ hrs in paper towel it popped and has a tail so put it in some soil!

Another Week Down – Things Are Moving Fast! Wow, another week flew by and we barely noticed! The plants are growing beautifully and developing nicely. One surprise this week: one of the Monkey Grease plants is definitely not a monkey... it’s a Gorilla Grease! 😄 This one is thriving — strong, fast-growing, and clearly the most vigorous of the whole bunch. It really stands out compared to the others, and we’re excited to see how it continues to develop! This week we also started light feeding: One watering with Fish Mix at 1ml/L (around 280 ppm) Followed by a watering with Alg-A-Mic at the same ratio (around 150 ppm) Both feedings were well-received, and the girls are loving it so far! 🌱💪

The hermaphroditic of the two S.A.D.s has stopped, in total it remained at 4x 🍌 Since 5 days no more were added and I could already observe via my timelapse recordings how the flowers continue to grow. The Bruce Banner also shows no further deterioration in terms of potassium deficiency, the flowers continue to grow steadily. Although the S.A.D.'s were fertilized identically, neither of them show any major deficiency. Also interesting how S.A.D. #4 has overtaken her sister and her smell is not "grassy" at all. Something between floral with a sweet citrus/orange note. What also surprised me was that the Bruce Banner is already very far along in the development of its trichomes, many are already milky!

I harvested Dorel and will soon do #4 as well. I had to as I don’t have the space to dry all 3 at once. Tbh Dorel didn’t look so good today, although I couldn’t see much amber trichromes she looks a bit overdone. Will probably do #4 this weekend, and Ozz the 2 following wees