8 weeks later and these girls are getting smelly! They are also steadily packing on some weight and I'm getting excited! This week the girls look to be in mid flowering stage and they were starting to show some kind of deficiency (maybe should've top dressed last week) well I top dressed last night and whipped up a compost tea for them too so we'll see how they react this week. Other then that a very light defoliation for air flow and tightened up the lst wires. Getting very excited for a taste 😋 Hope you all had a good week and thanks for checking in ✌️

This week I Realised I'd used the wrong feed chart so I was over feeding 🙄 the leaves burnt on a few plants so I've only fed them water this week and will resume feeding from next week. There are still no pre sex pistils showing but it can't be far off now. As soon as pistils start to show I'll start feeding bloom feed. I've turned the light up to 30000lux using HLG's online calculator for converting a lux meter/app reading to PAR , my light is 3000k which with lm301H Led's works out @ 465ppfd which is about the max the plants can use in veg without supliments co2. Here's the link for anyone using quantum boards, it has different spectrum calculators. It's been really useful setting my light up so big thank you to HLG (even though I have a Geeklight) and a thank you to the GML show on YouTube for bringing it to growers attention (I love that weekly show, so much info and perspective) https://horticulturelightinggroup.com/blogs/calculators/converting-lux-to-ppfd Hope everyone's staying safe and growing 😎

Day 29 - Looking great! I'm liking the Roots soil so far, very healthy looking plants. As the differences between LST and topping become more apparent I'm realizing I like topping more. The burst of growth to the side branches after topping does wonders for the structure of the plants. Pulled the two LST'd plants down a little lower and topped WWBB1 for the second (and probably last) time. Day 32 - Still right on track. Gotta start considering when to flip to flower as the sativas continue to take up vertical space.

April 4th 2024 Went on vacation for few days and came back to lil disappointment with one I was using the acinfinity bottom watering pot and one plant didn’t even drink from its water reservoir I have no clue why not and other did came back to empty pot so hopefully the water I watered with from top works that’s only problem I came back to other plant is going did some defoliation Welp today April 7th I messed around and found out what to much LST can do if ya to rough I cracked one plant right down the middle but with quick thinking got some tape and taped that hoe right up so hopefully I’m good besides that feed recharge today bout half gal each to scared to go full gallon don’t want to drown them

Awe horrible week got slight bit of bud rott was ill for few days and never attended to girls covid sucks but Hopefully still ok ✌️🏻

Licht habe ich gestern auf 70% hochgedimmt. Habe begonnen alle, außer der Amnesia, mit Hesi TNT zu füttern. Taste mich dabei langsam am die an die optimale Menge ran. Aktuell bekommt Lemon Orange die halbe empfohlene Dosis. KISS und Grapefuel Gelato bekommen etwas 20%. Bei der KISS werde ich die Dosis beim nächsten Mal noch mal erhöhen. Lemon Orange wird außerdem heute ihr erstes LST bekommen. Bei der Grapefuel Gelato gehe ich aktuell von einem Schaden durch die Hitze aus. Diese werde ich heute Abend wahrscheinlich Toppen. Die Hauptnodie wächst langsamer als manche Internodien. Die neuesten Blätter an der Hauptnodie sehen auch verkrüppelt aus und auch die der obersten Internodien. Die Blätter der unteren Internodien sehen soweit gesund aus, deshalb hoffe ich das Topping die Probleme löst.

Loved the strawberry pie, it smells sooo fruity and delicious and it smokes amazing definitely will have to grow this strain again !! Thank you fastbuds

I had to top Cantaloupe haze and White widow as they were too close to the lights and I didn't want to loose any ppfd on my lowers. My GSC seems perfect for not topping. next time I would top those strains at least once. I have been flushing the CH and getting run off around 1200ppm which seems like the max she can take the others like it stronger so I have been flushing them every 2-3 days. CH is tough to keep happy but will continue to monitor. All plants are in week 4 of flower 12/12 from seed.

H.Q. strain, love it . Thanks @fastbuds

This long journey is over, it's harvest time. Can't wait to taste the cheese. 💚🌱

Day 24: Watered each plant with 0.5L with nuts 1010 ppm, 2148 us/cm, 2.1 EC Day 26: Watered each plant with 0.75L with nuts 1045 ppm, 2223 us/cm, 2.2 EC

Ok so this week I did a defoliation and a tigh down too the netting , I really was not planning on doing such a big defoliation but the leafs became thick and dense and there was almost no light penitration down too the lower branches of the plant , so she is opened right up now and the humidity and temperature has dropped loads and the air is moving alot better around the leaves and branches , I will not take anything more off her now , I have also added Cal mag too the nutrients for this week only , she is well into pre flower now but the stretch has not yet started so I figured it's no or never too defoliate and tigh them down , this grill really has grown right out this week and is unrecognisable from the plant just a few days ago , I am very happy with them so far and being new too all this it's all very exciting , Thanks for looking :)

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to my Auto Cinderella Jack Diaries from Dutch-passion DAYS 57-63 flowering days 32-38. Both plants have left pre flower behind. And have put on some decent weight. Both girls are very healthy. Even with the constant stress of been dried out and again and again. Has caused some of my plants to hermie. But, these girls are OK. Checked them out very well. And females parts all round. Let soil dry out. And sprayed a 6% h202 on top of the soil to kill and larva or gnats that haven't gotten wings yet. And let's hope this will be the last week of these shits. Am going to push a much higher PK with my synthic booster on the synthic CJ. and for the other, I'm going to leave her be. She's not needing or wanting anything. Maybe another 2-3 more weeks max The organic stands at 58cm and side nodes can reach up to 40. She's a very bushy plant with a lot of bud sites. The Synthetic has a completely different structure. And looks like she was topped and trained hard to give off even cola base (was not the case) just the way she turned out. Thanks for viewing my diary. Drop a like or comment if you'd like me to visit your diaries. Either way, thanks for dropping by.

hello fellas have ya seen my clone that refuses to grow it is still alive making only three finger leaves i should kill it but i cant lol gonna see where that brings us looks like i am gone be in flower for about nine weeks THREE WEEKS TO GO i see the cola formation growing in am thinking if i would feed them this week they now have got three waterings plain water i guess i am gonna do that 600ml every other day biobizz + water

Easy grow and good advices from this site ! Thanks to your site, we can see other growers who are growing the same strain and its interesting to see different way of doing ! Thanks Growdiaries for this site and all this mix of knowledge : 1:0000

hello, quick update.. I got another fan which is amazing.. čubičky (my plants) looks healthy and that's most important.. <3

My first attempt of growing with my own DIY setup. Specs : - PC box 19x42x43cm (after a few weeks I changed it with a bigger carton box) - 4 x Bridgelux Vero 10, 3000K, 9.1 Watts, 80 CRI, 270mA, 143Lm/W - Mean Well HLG-60H-C350B 70W LED driver - 100k Linear Potentiometer for dimming lights - Aluminum profile 30x7cm for mounting LEDs - ESP32 Dev Board - DHT22 temperature & humidity sensor - 2 x Noctua-NF-S12A for ventilation with PWM control by microcontroller - 3lt pot - Blynk IoT app for remote control Started germination of 1 feminized Northern Lights seed from SensiSeeds using paper towel method. Sprayed with some water and store it in small plastic food container. Seed popped after 24 hours and after 48 it was ready for pot. Unfortunately didn't take any photos of the process.