Buenas noches familia, pues empezamos con el final. Nuestras power plant xL se han comportado fenómeno , recordamos que metimos 9 plantas de 3 variedades distintas y esta es la única variedad que perdimos espacio del indoor. Aun así sin palabras en cuanto al resultado. Planta con floración muy rápida, bastante vigorosa, lo malo es que al final tendrás que tutorar si o si, pero es por el peso de la flor. 56/59 días para cortar en floración, (y es Sativa).Se supone que tira 20%thc y su genética es South African sativa.

I will update this within 5 days . I still dry the flowers.

Friday, September 25, 2020 Chopped to dry in closet. No trimming needed Wednesday, September 30, 2020 Trimmed and jarred. Very little trimmings were thrown away. My first indoor grow was a success! I don't have the largest tent so it was nice to keep it short. Not the biggest yield in the world but it was a fun experience. My only regret is not watering or feeding it enough. I'm main-lining my other auto grows so we'll see how those do. Thanks! -JTGROW

Day 79 - Added 7 mL of CalMag Micro Grow and Bloom. Added 7 mL of each additive. PPM is 706. Day 81- Using noticeably less water now. Checked PPM 475. In moving the plant to water, a small branch broke. Trichomes are mostly clear. Started to dry it as a tester for rest of the plant. It takes 7 days for me to dry the tester at 70F and 40RH. Not ideal temp but that is how it goes. Day 82 - Might have seen first amber trichs Day 83 - Added 10 mL of Overdrive. PPM is 430. Trichs are mostly cloudy. Hairs are 80% orange. Branches are leaning from the weight. Day 86 - Added 5 mL of CalMag Micro Grow and Bloom. Added 5 mL of each additive. PPM is 588.

Hi Grower! Day 85 I let the damaged leafes that i can control a little whats going on!! She grows very well and smells very spicy!! Very nice Strain!!! AND STABILE!!! Growing under Mars Hydro TSL-2000 (75% power) When you want to grow with the same professional Equipment then Checkout this Link https://marshydro.eu/?ref=a3uvgodbbkhj Discount Code: Budspencer420 Follow me on Instagram for best Shots : bud_spencer_of_herbs

Week 14 for Gelato 41 by seedstockers This girl has absolutely loved the rain😇 It was very heavy rain too so the pot is easily saturated... honestly don't remember the last time i had to water her😂 was maybe 3/4 weeks ago & don't plan on doing so any time soon either. I tried to defoliate a little because she's getting overly bushy but found to be harder to do than i expected need her to gain some height so i can easily spot what to remove. Even though she looks sad after being sprayed down with water it will benefit her in the long run toughening up her branches from the weight when it comes to flower time. Need to measure her but she's not too far from the 2meters wide mark i was aiming for. No pest issues think the predator spiders are taking care of that for me😎

coming to the bitter end may be 2 more weeks until harvest. Not seeing a lot of bulk or growth. the fade is on, and the watering has been drastically reduced. lets see how it gose next week

Chop day today Day 80

Fed all four twice with half a gallon nute water, PH 6.3, day 43 and 49. Video and photos from day 50.

The buds shine like a shooting star mad sticky too⭐️ First thing that hits me with the smell straight up like canned pineapples in syrup🤤🍍

I think this is gonna be the last time I'm topping her, and I will start to apply some lst to make her a little wider in a couple days👨‍🌾💚🌲 Still only water and sometimes adding some Aptus 🌊💦

Fue una exelente cosecha, esta planta en un principio se tuvo en imddor y posteriormente sacada al exterior. Es mi primera experiencia plantando en suelo. La planta llego a un gran tamaño de la cual se cosechó una gran cantidad superando la cantidad promedio.

Harvest day First day of dry 10/2/25

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome back boys, A little update with those rock hard colas, the finish line is being seen not far away on the horizon. I hope they gonna fat a little bit, I think we all wish the big fattening deep down ... Stay tuned, stay stoned

Flowers will now fatten up Right before the pictures were taken i cut of lower thin branches and fanleaf all over the plant just enough to expose as many buds as possible

Plants seem to have responded well to the flushing between week 14-15. They were flushed for seven days with nothing but neutral water to help with the nute burn. New nutes were added after that. Fan leaves are dying and buds are fattening. Checked trichomes and I’m thinking I will begin flushing this week. I’m hoping for a 10-14 day flush unless I start seeing a lot of amber. I’m feeling torn and may harvest the purple trainwreck plants at two different times. There are a lot of cloudy trichomes but there’s still lots of white pistils being thrown out as well. Didn’t see much amber, some buds have more clear trichomes than others. The clone’s buds appear further along than the mother plant. I may flush only one plant this week and start the flush on the other in another week or so. The widow x plant isn’t ready to flush and is growing strong. I accidentally left my timer on “on” when I left during a weekend during week 3 of flower. The widow x plant started to reveg a bit from the 48 hours of light. I forgot this happened entirely and for a while couldn’t figure out why the buds weren’t fattening... but I’m fairly certain that is why that plant stalled so long. So far no hermies or males! That was several weeks ago. I’m now on week seven of flower so I’m hopeful for a completely seedless grow. I will continue adding photos throughout this week. Harvested the bottom branches off to make room for my next flowering plant. I couldn’t wait any longer, it’s getting too tall and I’ll run out of room. This is the clone that was taken the same time as the flowering PT clone. It has been in a veg tent since being removed from the flowering tent. Buds from lower stems are drying and look good so far... Wet weight- .75 ounces~ 21 grams Dry weight- ~.20 ounces~ 5.5 grams

Week 2 begins, showing some deficiency signs, hopefully the addition of big bud coco will help correct it.

Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. I put some pictures on the construction here in week one. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable theme in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 8 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. Today I have put my seeds in my moisture dome and hope the seeds have germinated within a few days. I am very excited to see how the new growroom will work and how this Blue cheese Auto from Royal queen seeds will turn out. Blue Cheese automatic cannabis seeds are a cross of Blueberry Automatic and Cheese Automatic that has been perfected over five years of breeding and careful selection. The result is a feminized, autoflowering strain with superior yield, flavor, and effects to either of its distinguished parent strains. THC: 16% CBD: Low Yield Indoor : 325 - 375 gr/m2 Yield Outdoor: 60 - 110 gr/plant Height Indoor: 40 - 70 cm Height Outdoor: 60 - 110 cm Flowering: 7 - 8 weeks Harvest month: 9-10 weeks after planting Genetic Background: Blueberry x Cheese x Ruderalis Type: Sa 35% In 40% Ru 25% Effect: Cerebral and uplifting Climate: Mild .............................................................................................................................................................................................................................................................................................................................................. 2017-09-11. Kl 12.00. Week 4 starts. I have cleaned the whole room for the new week and gave the girls water and nutes. Added videos and pics. Girl nr 1 is 27 cm high and girl nr 2 is 50 cm high and nr 3 is 15 cm high. ------------------------------------------------------------------------------- 2017-09-12. Kl 10.00. New pics and video. Nr 2 has grown 5 cm in 23 h, its incredible. --------------------------------------------------------------------------------------------- 2017-09-13. Kl 22.00. Everything is great in the garden right now. Updated with new video. ---------------------------------------------------------------------------------------------------------------------------- 2017-09-15. KL 10.00. New pics and videos. Girl Nr 1 is 33 cm high, Nr2 is 70 cm and girl Nr is 22 cm. Nr 2 has grown 20 cm the last 3 days. Look at the stem of girl Nr 2 😍 The girls are starting to smell a lot now. Its a intense smell in the grow room. ----------------------------------------------------------------------------------------------------------------------------------------- 2017-09-16. Kl 10.00. The girls grow like crazy, i have to defoliate about 20 leafs a day. Nr 2 is 76 cm tall now, i wonder when she slows down?. Added 2 liters of water and nutes this morning and 2 new videos. ------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2017-09-17. Made a time laps of the girls first 28 days.