Fractal week 17 7/ 22-28 This week has been rainy so the outside girls only got watered and fed once. with all the rain we've had, all the girls grew a bit. I top dressed all girls with Bloomin Soil from Elm Dirt. Photo Fractal is now 49 inches and stacking nicely. Happy girl means happy mom.

Dear team TrolMaster Europe, We would like to express our sincere gratitude for providing us with the Tent-X controller. We are delighted with its functionality and performance! We are confident that this controller has significantly aided us in our journey with our beloved plant Jealousy from Zamnesia.💪🤝 We were pleasantly surprised by the efficiency and ease of use of the Tent-X. It seamlessly integrated into our plant care system and helped us achieve incredible results. Once again, thank you very much for your support and high-quality equipment. We are immensely satisfied with your product and hope for long-term cooperation in the future. Today marks the end of our journey with Jealousy from Zamnesia💚, and we want to express our heartfelt gratitude to Zamnesia for their support throughout this entire journey. We also want to extend our thanks to Plagron for providing us with their high-quality fertilizers. Their products are not only effective but also incredibly easy to use. We highly recommend them to everyone!

PeaceAndLove🌴.

Welcome to the beginning of week 7 for Karen. Thank you for stopping by! I'm counting this as the first week of flowering but she's been sending out pistils for two weeks, so I guess that weeks four and five should have been flowering weeks. Sorry about that. Surprised to be here but excited to see the next few weeks. Day 44: This girl is amazing. She took the horrendous start I gave her for the first three weeks, and just exploded. Look at her now! If you are really interested, now look at Misty and Nesia - imagine what they could do! I am really pleased with Karen here, I think she has more surprises in store. I am very glad I decided to LST or she would be towering above everything else in the tent. I have noted early Mg deficiency, as more advanced on Nesia. Correcting with foliar and re-addition of CalMag to feed. Day 44.5: Fertigated approx 1.5l. Defoliated approx 10 leaves. Slightly adjusted LST. Day 46: Fertigated 2l. Karen is very thirsty. Her roots have breached the side walls at the bottom of the pot in 360 degrees... this is the biggest root system I've had on any potted plant to date. She is stretching fast now, I think by the end of next week she will be done stretching. I must get a photo of her stem. The base is ridiculously thick. Overall Karen is shockingly good compared to expectations from the poor start. I can only imagine how big she would be. She is currently 27cm tall and at least 50cm in diameter. I count at least a dozen colas. Day 47. She is now 29cm tall and pistils are bursting forth at a phrenetic pace. I have added a comparison shot of my grow space for size reference. Day 47.5: Defoliated approx 30 fan leaves. Slightly adjusted LST. Rearranged tent and cleaned floor. I have ordered some risers to raise the pots off the floor, this will make it easier to deal with run off. While I was inspecting Karen I had a good look at her roots. The fabric pot she is in has viewing flaps (they were designed for potatoes) and I had a look... every bit of the pot is full of roots, and they're all properly pruned. I see no circulation or binding. If Karen produces at anywhere near the rate she is supposed to then she might yield as much as 150g. Day 48.5: Rapid changes. Pistils everywhere, more stretching. Karen is very thirsty. Fertigated. Added pot risers and adjusted pot. Did a little LST to separate some bunched colas a bit. Had a really good inspect of the root system. Wow this plant is a beast. Day 48.75: Fertigated 3l Day 49: Height 34cm Diameter: 60cm. End of week summary. Really big week, she is stretching quicker and quicker. Today she has developed a striking scent. I will now have to keep the tent closed so temps will rise. Day 49.5: Removed about 10 fan leaves. On reflection back over this week I think I needed to be taking a top and side photo, so I shall do that from next week.

Ok so I asked about harvest to you wonderful folks, and decided to chop. I've left PPP3 as she isn't quite at the same stage as the other 2 yet. So I used the old microwave drying trick. I remembered to put it in for 1 minute on a piece of kitchen towel- Please do NOT do this! It caught the paper and weed on fire and I had to put it out in the sink. (It was only in there for about 15 seconds). I tried again using a couple seconds at a time. It worked out that time. I smoked some up, and it's very nice. Tastes peppery and exotic. Smells divine like dank usually does. 7.3.25: Buds we're ready to trim, I think slightly too dry. Could have done it yesterday, probably. Weight dry with stems was 1 and 7/8 of an ounce. I'm putting it in my mason jar

Yessir! Just transplanted. 2 7gallons 2 5 gallons 2 2 gallons 1 - 2 Weeks till flower

Week 28 december - 3 January Happy new year

Got the trellis net up, got about an inch of new cola growth, should be long enough to bend soon...might top agian at that point. DLI is at 47 light at 70 percent. Adjusted light height to dli and Upped to 7 this am.

I was very surprised at the remarkable growth of both plants. Tree 1 gives me a clear view of the superiority of height compared to the previous week. The copper buds clearly show the enormous size, I am trying to increase the amount of nutrition to promote the maximum growth in size and taste.

Week 5

What can I say mimosa cake auto still frosty and thick as ever which I could’ve had her dialed in sooner truly think this could be an amazing Grow cannot wait to grow her again nice dense buds frosty cola smells of mimosa cake. 🔥💯

Molto aromatica con sentori di frutta pungente e spezie confuse con il caffè molto gradevole

This two were part of another diary and got moved out due to space reasons at VW8 and moved back indoors at VW20 https://growdiaries.com/diaries/218151-auto-god-s-glue-grow-journal-by-yan402 13.09.25 VW21 Both are looking good and are developing tighter nodes than when they were outside, I'm going to have to keep cutting them back every once and a while I also applied nematodes against thrips and fungus gnats. 20.09.25 VW22 some spots and some yellow leafs, I think it's a root problem, probably root bound, but both seem healthy and are getting thicker so maybe just top up with Coco coir and give a slight different nutrient schedule less Tri Part Bloom could do the trick MAYBE lol. 27.09.25 VW23 Topped up the pots with extra coco coir and trimmed the side roots a bit, both plants were root bound 📹 03.10.25 VW23 did a HST/LST session 📹 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 Day to day tasks & actions 🌿 🌱💦🌱💦🌱💦🌱💦🌱💦🌱 11.10.25 VW24 – Fed 3l of #1 → 2l runoff (*RUNOFF reused for tomato plants) 🍶💧🍶💧🍶💧🍶💧🍶 💧 Nutrients in 30L #1 🍶💧🍶💧🍶💧🍶💧🍶 💧 TriPart Micro: 10 → 30 → 40 ml = 0.33 → 1.00 → 1.33 ml/L 🍶 TriPart Grow: 0 ml = 0.00 ml/L 💧 TriPart Bloom: 10 → 30 → 20 ml = 0.33 → 1.00 → 0.67 ml/L 💧 Cal-Mag: 60 ml = 2.00 ml/L 🍶 Home-made FFJ/FPJ (new fish batch): 30 ml = 1.00 ml/L 💧 pH Down: Citric acid (BuxXtrade) — adjust to ~pH 6.0 📦 TOTAL : = 160 ml per 30L = 5.33 ml/L 🍶💧🍶💧🍶💧🍶💧🍶 ⚙️✂️⚙️✂️⚙️✂️⚙️✂️⚙️ ✂️ Tools & equipment ✂️ ⚙️✂️⚙️✂️⚙️✂️⚙️✂️⚙️ ✂️ 2× MarsHydro SP3000 ⚙️ MarsHydro 150mm ACF Ventilator ✂️ Trotec dehumidifier (big unit) ⚙️ Mini no-name dehumidifier ✂️ Kebab skewers (LST – stainless) ⚙️ Wire + roast skewers (LST assist) ✂️ Scissors (HST) ⚙️ Vacuum (for spills & cleanup) ✂️⚙️✂️⚙️✂️⚙️⚙️✂️⚙️✂️⚙️✂️⚙️ 🍒🍭🍬🌈🍒🍭🍬🌈🍒🍭🍬🌈🍒 🦄Fantasy Feast ( Seeds)🦄 🌈🍒🍭🍬🌈🍒🍭🍬🌈🍒🍭🍬🌈🍒 Species: Hybrid (Regular) Genetics: The mother is Unicorn Whip by Dirty Bird Genetics. The father is Charcuterie by Cannarado Genetics. Effect: Unknown Mixed effect body and head high Flavor: Some phenos are Skunky gassy fruity, some are fruity sour citrus with a chemical touch and a touch of skunk Flowering: Estimated 8–10 weeks Resistance: Strong — Testing phase done

The harvest window was calculated to start next week. However, I've been monitoring the trichomes under a microscope and the majority of top buds had a nice mix of amber triches, I had to begin harvesting a week early. This week I began harvesting the topmost buds only in order to allow the lower ones to continue to develop and mature. We certainly had a few hearty colas, with a number of well defined buds throughout all three plants. Next week we will harvest the remaining as initially estimated.

is in its exact point to be cut ... she grew healthy ... with cloudy days ... the substrate I did ... was fed every 15 days with guano and worm humus only ... amber tricomas 5% and mostly cloudy

Hello again! Into week 7+ ladies were very bushy so I decided to dive in and clear out the lower canopy and removed old leaves. As usual watering every 4-5 days and I have increased the amount of water to about 8L to each pot. They are drying out faster day by day. Still using 50mL of molassas to 5gal of water pH to 5.9 this time. No issues to report. See you on the next update!

7/21 330am MONDAY everyone is looking good. I gave a little bit of nitrogen from a germination grow yesterday gorilla gas F1 has little white bugs in the soil, but it doesn’t seem to be affecting anything too bad. I don’t want to spray anything. It will affect the outcome and make everything taste gross. I learned that the hard way☠️ flushing matters because even though the flowers process & convert, there is still water being delivered up through the root system that carries up things that taste bad and your BONG WATER will look the same color as your runoff SHOULD HAVE after the first hit. DONT DO THAT, DONT BE LIKE ME!👍👍 7/22 3:22 AM I got five Mike Tyson clones. They are not autos, but I think they are gelato. They are great. I put some extra dirt and one of the Mike Tyson plants yesterday so I have to put in some new nutrients on the nitrogen deficiency, everything‘s looking good. 👍👍. 7/22 1030pm......WELL WE KNOW WHAT DOESNT WORK...... I SCREWED UP WITH RAPID START. IT WILL CAUSE VERY SPECIFIC BURNS TO THE LEAF TIPS AND SIDES WHEN USED. I MAY BE USING TOO MUCH, 0.5ML SHOULD USE .25ML, WONT USE AGAIN UNTIL I FIGURE OUT WHATS INTERACTING AND CAUSING THIS. The clones are looking great, they will go back from flower to veg this week then a month of slow germination and kept low and slow so I can keep them. 7/23 4 AM adding TMG powder to the mix 👍👍 7/24 111am TMG did not harm the plants. The clones are progressing nicely. There may be some problems mixing methylene blue with nitrous oxide but it’s maybe different. There’s a combination of amino acids. I’m still trying to work out. Proline arginine citrulline are the main ones right now that have pretty good results. Methylene blue works really good during veg not so sure during flower I tend to accumulate in the chloroplast and on the in the leaves you can see it. It doesn’t affect the flavor or the taste. I’ve smoked some critical Kush shake on some pretty heavy methylene blue is an experiment and I’m not dead yet so dammit here we are.👍👍👍🎉🎉. My personal results using this combination on myself has been phenomenal. The change after using the system on myself is nothing I’ve experienced before I feel ripped off by about 40 years of garbage medicine when I created something that actually worked for the first time, I wouldn’t put too much faith in any more PhD lab coats. They are all failures and I found a system that works.👍. 845am PROP WALL UPDATE, I GET DISTRACTE....OH LOOK AT THAT... I DONT KNOW WHERE I SAID DISNEY WHILE SHE WAS LISTING BUT IT SCREWED UP OR MADE INCREDIBLY BETTER THE INFO YOU ARE ABOUT TO READ!!!!!!! IM PRETTY SURE I SAID DIXIE VIBE...BUT OK 7/24 315pm Got it, so anyway, we were talking about adjusting the dosage for your mix on flowering cannabis, and you’re saying to skip the methylene blue. Let’s pivot and finalize the analysis without methylene blue, focusing on 10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg) in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage (4–8 mL/L). I’ll wrap up the effects on bud quality, cannabinoids, and terpenes during the flowering stage (weeks 4–12), and give you practical tips to optimize your cannabis grow. ### Effects During Flowering Stage (Without Methylene Blue) 1. Amino Acid Quick-Sorb (10 drops, ~100 mg in 2 L, ~50 mg/L): - Bud Impact: At 50 mg/L, amino acids (glycine, L-arginine, L-proline, etc.) act as biostimulants, boosting terpene production but potentially reducing cannabinoid content. They increase nitrogen and sulfur uptake, supporting trichome development but may lower calcium and iron, risking deficiencies that affect bud density. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Cannabinoids: Reduces CBDA by up to 44% and THCA by up to 196% (especially in drain-to-waste systems), lowering potency. Recirculation systems mitigate this loss slightly. (https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full) - Terpenes: Boosts limonene (81–123%) and β-myrcene (139–167%), creating aromatic buds like a burst of citrusy magic from a Disney parade. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/) - Flowering Note: The low dose minimizes nitrogen overload, but excess could delay bud ripening, making buds leafy like overgrowth in Sleeping Beauty’s castle. Monitor for yellowing leaves (calcium/iron deficiency). 2. Trimethylglycine (TMG) Powder (1/4 teaspoon, ~1250 mg in 2 L, ~625 mg/L): - Bud Impact: TMG acts as an osmoprotectant, enhancing stress tolerance (e.g., high light, humidity swings), which is key for robust bud development in flowering. It supports methylation, potentially upregulating terpene or flavonoid genes, but doesn’t directly boost cannabinoids. (https://en.wikipedia.org/wiki/Trimethylglycine) - Cannabinoids: May indirectly support THCA/CBDA synthase via methylation, but evidence is speculative. No significant direct impact. - Terpenes: Likely amplifies amino acid-driven terpene boosts, enhancing aroma like a floral float at Epcot’s Flower and Garden Festival. - Flowering Note: At 625 mg/L, TMG helps plants thrive under stress but may increase nitrogen levels, risking delayed flowering if overdone. Watch EC to avoid burn. 3. Nitric Boost Capsule (1 capsule, 1400 mg total NO precursors in 2 L, ~700 mg/L): - Ingredients Recap: L-arginine (400 Great! So, let’s finalize the flowering cannabis analysis, focusing on your mix—10 drops Amino Acid Quick-Sorb, 1/4 teaspoon TMG powder, 1 Nitric Boost capsule (with L-arginine 400 mg, L-arginine AKG 400 mg, L-citrulline 200 mg, L-citrulline malate 200 mg, L-citrulline AKG 200 mg), and 1 drop 5% methylene blue in a 2-liter solution with Advanced Nutrients Connoisseur at the recommended dosage. I’ll finish the terpene effects, summarize the overall impact, and give you actionable tips to optimize bud quality, cannabinoid, and terpene production during the flowering stage (weeks 4–12 for most cannabis cultivars). Then, I’ll tie in any Disney vibe you’re feeling, maybe with a sprinkle of fun! — ### **Completion of Effects During Flowering Stage** We were discussing the **terpene profile** and how the mix impacts bud quality. To recap: amino acids (Quick-Sorb) reduce cannabinoids (THCA, CBDA) by up to 44–196% but boost monoterpenes like limonene (81–123%) and β-myrcene (139–167%), TMG enhances stress tolerance, Nitric Boost increases NO for nutrient uptake and terpenes, and methylene blue’s low dose (1.25 mg/L) may slightly counteract NO effects or provide antimicrobial benefits. Let’s finish the terpene section and synthesize the rest. - **Terpene Profile (Continued)**: - **Amino Acid Quick-Sorb**: The significant increase in limonene and β-myrcene enhances bud aroma and therapeutic value (e.g., anti-inflammatory, sedative effects). These terpenes are concentrated in trichomes, improving market appeal, especially for strains prized for scent (e.g., OG Kush, Blue Dream). Other amino acids like glycine and serine may support terpene synthase enzymes indirectly via protein synthesis. - **TMG**: By aiding methylation, TMG could upregulate terpene biosynthesis genes, amplifying the amino acid effect, though direct evidence is limited. - **Nitric Boost**: The NO precursors (L-arginine, L-citrulline, totaling 1400 mg per capsule, or ~700 mg/L in 2 L) boost NO signaling, which upregulates terpene pathways, synergizing with amino acids for a stronger aroma profile. - **Methylene Blue**: Its NO inhibition might slightly dampen terpene enhancement from Nitric Boost, but at 1.25 mg/L, the effect is negligible. Its antimicrobial properties could protect trichomes from fungal issues (e.g., bud rot), preserving terpene quality. - **Advanced Nutrients Connoisseur**: Its humic acids and organic inputs enhance terpene production, complementing the amino acid and NO effects for a robust flavor profile. (https://pmc.ncbi.nlm.nih.gov/articles/PMC9008891/, https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2022.868350/full, https://www.advancednutrients.com/products/) — ### **Overall Impact on Flowering Cannabis** - **Bud Quality and Yield**: - **Positives**: The mix enhances terpene production (limonene, β-myrcene), improving bud aroma and appeal, ideal for premium markets. TMG and proline (from Quick-Sorb) boost stress tolerance, supporting bud development under high light, heat, or humidity stress, common in flowering. Connoisseur’s optimized NPK (e.g., ~194 mg/L N, 59 mg/L P) ensures dense, resinous buds. Nitric Boost’s NO precursors may increase nutrient delivery, potentially increasing bud size in early flowering (weeks 4–6). - **Negatives**: Amino acids risk reducing THCA and CBDA by 44–196%, especially in drain-to-waste systems, lowering potency. Excess nitrogen from Quick-Sorb and TMG could delay flowering or produce leafy buds, reducing density. Methylene blue’s NO inhibition may counteract Nitric Boost’s benefits, potentially limiting nutrient uptake or terpene gains. High EC (estimated 1.8–2.8 mS/cm with additives) risks nutrient burn, visible as brown leaf tips or stunted buds. 7/25 9am Water today no MB, all aminos tho. 217pm GOATS sustained minor damage from Rapid Start product experiment at the wrong time. It's ok on its own in the beginning with clones too but never with flowering nutrients. G Gas f1 and GC Auto have more damage because I did not start Amino and Methelyene Blue treatment, so it does work in Veg. and creates a protective environment that carries through till the end. No more MB or Aminos, I WILL keep using small amounts of TMG Powder. and I will get all the other stress amino acids separately , just AN and ATAMI at .5ml per TWO liter, I do EVERYTHING in 2 liter now, just easier and more accurate. NEVER FUCKING SLEEP...FUCK 745PM.... AMINO'S HAVE BETRAYED ME!!!!!! FARGING BASTAGES!!!! I think I will get all the stress aminos separately from BulkSupplements they have small bags of each one but its 18 of them and about 12 to 17 each.....BulkSupplements. 137am. Something smoking Purple Haze...puts me to sleep....nooooo. ZUP by Fastbuds smell like. garlic cheese muffin its just greatl. I got over nitrogen and Frost Banger was leafy, all were small and all turned out perfect smell and taste. 3am they all got pretty burned by the rapid start and it also could’ve been. I sprayed a little bit of Nemo on the dart when I saw the bugs they bad idea, but I had to do it. 7/27 1233am. IVE SEEN RE-VEGES YOU PEOPLE WOULDN'T BELIVE ... UNTIL NOW LOSERS.. the Tyson plants are re-vegging perfectly!!!! in a couple months they will be strong and small. The G Gas F1 clones are ready but I think im going to try and bonsai tree them sounds like a fun project. OR EXTREMELY FRUSTRATING AND I THROW MOST OF IT AGANST THE WALL AND THEN BREAK STUFF. NOT SURE ITS WORTH IT. 7/27 230pm Adding proline to a 2-liter nutrient solution containing Advanced Nutrients pH Perfect Bloom (2–4 mL), Atami Bloombastic (1 mL), and betaine anhydrous (250–500 mg, 1–2 mM) for autoflowering cannabis plants could potentially enhance stress tolerance and growth, as proline is another well-documented osmoprotectant and stress-mitigating compound in plants. However, its effects on autoflowers, which are sensitive to nutrient overload, must be carefully considered to avoid imbalances. Since no cannabis-specific studies exist for proline or its combination with betaine, the following analysis extrapolates from research on other plants (e.g., alfalfa, peppers, maize) and accounts for autoflowers’ unique characteristics (short life cycle, low nutrient tolerance). Background on Proline • What is Proline?: Proline is an amino acid that accumulates naturally in plants under stress (e.g., drought, salinity, temperature extremes) to act as an osmoprotectant, stabilize proteins and membranes, and scavenge reactive oxygen species (ROS). It complements betaine’s role as an osmolyte and stress mitigator. • Role in Plants: Exogenous proline application (via nutrient solution or foliar spray) can enhance stress tolerance, improve photosynthetic efficiency, and support growth under adverse conditions. It also regulates gene expression related to stress response and nutrient uptake. • Relevance to Autoflowers: Autoflowers, with their compact size and 6–12-week life cycle, are hardy but sensitive to overfeeding. Proline could enhance resilience in stressful environments (e.g., high EC, temperature swings, or inconsistent watering), but excessive doses risk nutrient burn or metabolic disruption. Potential Effects of Adding Proline to the Nutrient Solution Based on studies of proline’s effects on other plants and the context of your nutrient mix, here are the likely impacts on autoflowering cannabis: 1 Enhanced Stress Tolerance: ◦ Osmotic Regulation: Like betaine, proline maintains cellular water balance under stress (e.g., drought, salinity). In maize, exogenous proline (1–10 mM) increased leaf water content and reduced wilting under drought stress. For autoflowers, proline could complement betaine’s osmotic protection, particularly in hydroponic systems or outdoor grows with variable conditions. ◦ Antioxidant Defense: Proline reduces oxidative stress by scavenging ROS and upregulating antioxidant enzymes (e.g., superoxide dismutase [SOD], peroxidase [POD]). In alfalfa under salt stress, proline accumulation (alongside betaine) decreased malondialdehyde (MDA) levels, indicating less cell damage. This could protect autoflower leaves and buds from stress-induced damage, especially under high-intensity lighting or heat. 2 Synergistic Effects with Betaine: ◦ Complementary Osmolytes: Proline and betaine both act as compatible solutes, stabilizing proteins and membranes. In Suaeda salsa, combined accumulation of proline and betaine under salinity improved growth and ion homeostasis (Na+/K+ balance). For autoflowers, adding proline to betaine could enhance stress resilience, particularly if grown in challenging conditions (e.g., high EC from Bloom/Bloombastic, low humidity, or temperature fluctuations). ◦ Potential Redundancy: Since betaine (250–500 mg, 1–2 mM) already provides osmoprotection, additional proline may offer diminishing returns in optimal conditions (e.g., controlled indoor grows with 18–20 hours light, 70–80°F, 40–60% humidity). However, under stress, their combined effects could be additive, improving root activity and biomass. 3 Improved Growth and Photosynthesis: ◦ Growth Enhancement: In peppers, 5 mM proline increased plant height and fresh weight under salinity stress. For autoflowers, proline could support vegetative growth (weeks 2–4) or early flowering (weeks 4–6), potentially increasing bud sites or yield, though effects may be limited by their short cycle. ◦ Photosynthetic Efficiency: Proline protects photosystem II and increases chlorophyll content. In wheat, 10 mM proline improved Fv/Fm (photochemical efficiency) under drought. For autoflowers under high-intensity lights (e.g., LED, HPS), proline could stabilize photosynthesis, complementing betaine’s effects on chlorophyll gene expression (e.g., Capsb genes in peppers). 4 Nutrient Uptake and Metabolism: ◦ Ion Homeostasis: Proline enhances K+ uptake and reduces Na+ toxicity under salinity, as seen in Suaeda salsa. This could improve nutrient absorption in autoflowers, which rely on efficient uptake due to smaller root systems, especially in hydroponics with Advanced Nutrients Bloom (high N-P-K) and Bloombastic (high P-K). ◦ Metabolic Support: Proline supports protein synthesis and enzyme stability, potentially enhancing metabolic efficiency during the rapid autoflower life cycle. However, excessive proline could disrupt nitrogen metabolism, as it’s an amino acid competing with other nitrogen sources in the nutrient solution. 5 Potential Impact on Yield and Quality: ◦ Yield: Proline’s stress-mitigating effects could indirectly increase bud weight by reducing environmental setbacks, similar to betaine’s potential to enhance biomass. Autoflowers yield 28–112 g/plant indoors, and proline might support larger buds if stress is a limiting factor. ◦ Cannabinoid/Terpene Profile: No data exists on proline’s effect on THC, CBD, or terpenes. It’s unlikely to directly alter cannabinoid synthesis but could improve plant vigor, potentially leading to denser buds or enhanced resin production. Overapplication risks altering flavor if not flushed properly. Recommended Proline Dosage • Optimal Concentration: 1–2 mM (230–460 mg per 2 L). ◦ Calculation: Proline molecular weight: 115.13 g/mol. 1 mM = 0.001 mol/L × 115.13 g/mol = 0.11513 g/L = 115.13 mg/L. For 2 L at 1 mM: 115.13 mg/L × 2 L = 230.26 mg (rounded to 230 mg). For 2 L at 2 mM: 115.13 mg/L × 2 × 2 L = 460.52 mg (rounded to 460 mg). ◦ Rationale: Studies (e.g., maize, peppers) show 1–10 mM proline is effective for stress tolerance, with 1–5 mM optimal for root application to avoid toxicity. For autoflowers, 1–2 mM aligns with betaine’s 1–2 mM (250–500 mg), ensuring compatibility and minimizing nutrient overload. Start with 230 mg (1 mM) to test plant response, increasing to 460 mg (2 mM) if no stress signs (e.g., leaf burn, stunted growth) appear after 1–2 applications. • Comparison with Betaine: Proline and betaine have similar molar masses (115.13 vs. 117.15 g/mol), so 1–2 mM of each results in comparable mass (230–460 mg for proline, 250–500 mg for betaine). Their combined effect should stay within autoflower EC limits (1.2–1.8 mS/cm for flowering). Application Guidelines • Nutrient Solution Recipe (2 L): ◦ Water: 2 L (RO or dechlorinated tap water). ◦ Advanced Nutrients pH Perfect Bloom: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). ◦ Atami Bloombastic: 1 mL (0.5 mL/L, suitable for flowering autoflowers). ◦ Betaine Anhydrous: 250–500 mg (1–2 mM, start with 250 mg). ◦ Proline: 230–460 mg (1–2 mM, start with 230 mg). ◦ Mixing: Add Bloom first, then Bloombastic, betaine, and proline, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). • Timing: Apply during vegetative (weeks 2–4) or early flowering (weeks 4–6) to support stress tolerance and growth. Stop both betaine and proline 2 weeks before harvest to allow flushing for optimal bud flavor. • Frequency: Use with each nutrient feeding (e.g., every 2–3 days in hydro, weekly in soil). Alternate or reduce frequency if EC approaches 1.8 mS/cm. • Source: Use L-proline (plant-grade, 99%+ purity) from a reputable supplier (e.g., agricultural or lab-grade sources). Avoid impure or non-plant-specific products. Potential Risks and Considerations • Nutrient Overload: Combining betaine (250–500 mg) and proline (230–460 mg) with Bloom and Bloombastic increases EC and nitrogen load (proline is an amino acid). Monitor for nutrient burn (brown leaf tips, clawing). If symptoms appear, reduce both to 0.5 mM (125 mg betaine, 115 mg proline per 2 L) or skip a feeding. • EC Impact: Betaine and proline each add ~0.1–0.2 mS/cm at 1–2 mM. With Bloom (1–2 mL/L) and Bloombastic (0.5 mL/L) at ~1.0–1.5 mS/cm, the total EC should stay below 1.8 mS/cm. Measure after mixing. • Redundancy: Betaine and proline have overlapping roles (osmolytes, antioxidants). In optimal conditions, adding proline may offer minimal additional benefits over betaine alone, but under stress (e.g., high heat, low humidity), their synergy could enhance resilience. • Cost vs. Benefit: Proline is more expensive than betaine and may not justify the cost in well-managed grows. Test its impact on a small scale first. • Flushing: Cease both betaine and proline in the final 1–2 weeks to avoid residual taste in buds, using plain water or a flushing agent (e.g., Advanced Nutrients Flawless Finish). • Unknown Cannabinoid Effects: No evidence suggests proline or betaine alters THC, CBD, or terpenes, but overapplication could affect flavor if not flushed. Summary of Effects Adding proline (230–460 mg, 1–2 mM) to a 2-liter solution with betaine (250–500 mg, 1–2 mM), Advanced Nutrients Bloom (2–4 mL), and Atami Bloombastic (1 mL) could enhance autoflower stress tolerance by: • Improving osmotic regulation and antioxidant defenses, complementing betaine’s effects. • Supporting photosynthesis and growth under stress (e.g., salinity, heat, low light), potentially increasing biomass or bud yield. • Enhancing nutrient uptake efficiency, critical for autoflowers’ small root systems. Start with 230 mg proline (1 mM) and 250 mg betaine (1 mM), increasing to 460 mg proline (2 mM) and 500 mg betaine (2 mM) if plants tolerate well. Apply during vegetative or early flowering, monitor EC (1.2–1.8 mS/cm), and stop 2 weeks before harvest. Risks include nutrient burn and redundancy, so test cautiously and ensure proper flushing. If you provide your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine the dosing or application method. Let me know if you need sourcing tips for proline or further guidance!”” 7/27 3PM. Adding **0.5 mL of Advanced Nutrients Rhino Skin (potassium silicate)** to a 2-liter nutrient solution containing **Advanced Nutrients pH Perfect Bloom (2–4 mL)**, **Atami Bloombastic (1 mL)**, **betaine anhydrous (250–500 mg, 1–2 mM)**, and **proline (230–460 mg, 1–2 mM)** for autoflowering cannabis plants could provide additional benefits, particularly in strengthening plant structure and enhancing stress tolerance. However, the effects must be evaluated carefully given autoflowers’ sensitivity to nutrient overload and the complex nutrient mix already in use. Below, I analyze the potential effects of adding 0.5 mL of Rhino Skin, based on the role of silica in plant physiology, studies on cannabis and other crops, and the specific context of your nutrient solution. ### **Background on Silica (Advanced Nutrients Rhino Skin)** - **What is Rhino Skin?**: Advanced Nutrients Rhino Skin is a potassium silicate supplement (typically 0-0-0.4 N-P-K, with ~4–7% silica as SiO₂). Silica strengthens plant cell walls, improves stress resistance, and enhances nutrient uptake. The recommended dosage is 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L) due to their sensitivity. - **Silica in Plants**: Silicon (Si), absorbed as silicic acid, deposits in cell walls as silica gel, increasing structural rigidity. It also mitigates abiotic (e.g., drought, heat) and biotic (e.g., pests, fungi) stresses and can improve photosynthesis and yield. In cannabis, silica is not essential but is considered a beneficial element. - **Autoflower Context**: Autoflowers have a short 6–12-week life cycle and smaller root systems, making them prone to nutrient burn if EC (electrical conductivity) exceeds 1.2–1.8 mS/cm during flowering. Rhino Skin’s low dosage (0.5 mL per 2 L, or 0.25 mL/L) minimizes this risk while complementing betaine and proline’s stress-mitigating roles. ### **Potential Effects of Adding 0.5 mL Rhino Skin** Based on silica’s effects in cannabis and other plants (e.g., rice, tomatoes, hemp), and considering your nutrient mix, here are the likely impacts on autoflowering cannabis: 1. **Strengthened Plant Structure**: - **Cell Wall Reinforcement**: Silica deposits in cell walls, increasing stem and leaf rigidity. In hemp (*Cannabis sativa*), silica supplementation (e.g., 1–2 mM Si) thickened stems and improved mechanical strength. For autoflowers, which are compact (1–3 feet tall), 0.5 mL Rhino Skin could strengthen branches, supporting heavier buds during flowering (weeks 4–10), reducing the risk of snapping under weight or wind (outdoor grows). - **Benefit in Your Mix**: Betaine and proline enhance osmotic and metabolic resilience, but they don’t directly strengthen cell walls. Silica complements their effects by providing structural support, potentially allowing autoflowers to allocate more energy to bud development. 2. **Enhanced Stress Tolerance**: - **Abiotic Stress**: Silica mitigates drought, heat, and salinity stress by reducing transpiration and stabilizing cell membranes. In tomatoes, 1 mM silica improved water retention under drought. For autoflowers, 0.5 mL Rhino Skin (~0.1–0.2 mM Si, based on typical 4–7% SiO₂ content) could enhance resilience in challenging conditions (e.g., low humidity, high temperatures, or high EC from Bloom/Bloombastic). - **Biotic Stress**: Silica forms a physical barrier in cell walls, deterring pests (e.g., spider mites) and pathogens (e.g., powdery mildew). In cannabis, silica reduced fungal infections in controlled studies. This could protect autoflowers, especially in humid indoor grows or outdoor settings prone to pests. - **Synergy with Betaine and Proline**: Betaine and proline reduce oxidative stress (via antioxidant enzymes like SOD, POD) and maintain osmotic balance. Silica adds a physical and physiological layer of protection, creating a robust stress-response system. For example, in *Suaeda salsa*, betaine and proline improved ion homeostasis under salinity, while silica in rice enhanced K+ uptake, suggesting complementary effects. 3. **Improved Photosynthetic Efficiency**: - **Leaf Support and Light Capture**: Silica strengthens leaf tissues, keeping them erect for better light exposure. In rice, 1.5 mM silica increased chlorophyll content and photosynthetic rates. For autoflowers under high-intensity lights (18–24 hours), 0.5 mL Rhino Skin could optimize photosynthesis, complementing betaine’s role in protecting photosystem II (e.g., upregulation of Capsb genes in peppers). - **Relevance to Your Mix**: Advanced Nutrients Bloom supports flowering with N-P-K, and Bloombastic boosts bud density with high P-K. Silica could enhance energy allocation to buds by improving leaf health, especially if plants face light stress (e.g., inconsistent outdoor light). 4. **Nutrient Uptake and Compatibility**: - **Enhanced Nutrient Absorption**: Silica improves root uptake of nutrients like potassium and calcium. In maize, 1 mM silica increased K+ and Ca²⁺ levels. For autoflowers, this could optimize absorption of Bloom’s macronutrients and Bloombastic’s P-K, critical for flowering. - **pH and EC Impact**: Rhino Skin’s potassium silicate can raise pH slightly, but Advanced Nutrients pH Perfect Bloom (2–4 mL) stabilizes pH at 5.5–6.5 (hydro) or 6.0–6.5 (soil). At 0.5 mL per 2 L (0.25 mL/L), Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), keeping the total EC (Bloom + Bloombastic + betaine + proline + silica) within 1.2–1.8 mS/cm for flowering autoflowers. - **Compatibility with Betaine and Proline**: Betaine (1–2 mM) and proline (1–2 mM) are neutral organic compounds that don’t chemically interact with silica (an inorganic compound). Their combined use should be synergistic, as they target different stress-response pathways (osmotic vs. structural). 5. **Potential Impact on Yield and Quality**: - **Yield**: Silica’s structural and stress-mitigating benefits could increase bud weight by supporting healthier plants. In hemp, 1 mM silica increased biomass by 10–15%. For autoflowers (28–112 g/plant indoors), 0.5 mL Rhino Skin might modestly boost yield, especially in stressful conditions, by complementing betaine and proline’s growth-enhancing effects. - **Cannabinoid/Terpene Profile**: No direct evidence links silica to THC, CBD, or terpene changes in cannabis, but stronger plants may produce denser buds or more resin. Silica’s pest resistance could reduce the need for chemical treatments, preserving terpene quality. Proper flushing remains critical to avoid flavor impacts. ### **Recommended Dosage and Application** - **Dosage**: **0.5 mL Rhino Skin per 2 L (0.25 mL/L)** is optimal. - **Rationale**: Advanced Nutrients recommends 2 mL/L for photoperiod cannabis, but autoflowers require 1/4 to 1/2 strength (0.5–1 mL/L). At 0.5 mL per 2 L, Rhino Skin provides ~0.1–0.2 mM silica (assuming 4–7% SiO₂), aligning with effective doses in plant studies (0.5–2 mM) while minimizing EC and pH impacts. - **Confirmation**: Your specified 0.5 mL is appropriate for autoflowers, ensuring safety with the nutrient-rich mix (Bloom, Bloombastic, betaine, proline). - **Nutrient Solution Recipe (2 L)**: - **Water**: 2 L (RO or dechlorinated tap water). - **Advanced Nutrients pH Perfect Bloom**: 2–4 mL (1–2 mL/L, start with 2 mL for early flowering). - **Atami Bloombastic**: 1 mL (0.5 mL/L, suitable for flowering). - **Betaine Anhydrous**: 250–500 mg (1–2 mM, start with 250 mg). - **Proline**: 230–460 mg (1–2 mM, start with 230 mg). - **Advanced Nutrients Rhino Skin**: 0.5 mL (0.25 mL/L). - **Mixing**: Add Bloom first, then Bloombastic, betaine, proline, and finally Rhino Skin, stirring thoroughly after each addition. Check EC (target 1.2–1.8 mS/cm) and pH (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Timing**: Apply from vegetative (weeks 2–4) through mid-flowering (weeks 4–8) to support structure and stress resistance. Stop silica, betaine, and proline 1–2 weeks before harvest (weeks 8–10) for flushing to ensure bud flavor. - **Frequency**: Use with each nutrient feeding (every 2–3 days in hydro, weekly in soil). If EC nears 1.8 mS/cm, reduce Bloom to 2 mL or alternate silica applications. ### **Potential Risks and Considerations** - **Nutrient Overload**: The mix (Bloom + Bloombastic + betaine + proline + silica) is nutrient-dense. At 0.5 mL, Rhino Skin adds minimal EC (~0.05–0.1 mS/cm), but the total EC (1.2–1.8 mS/cm) must be monitored to avoid burn (brown leaf tips, clawing). If symptoms appear, reduce Bloom to 2 mL or skip silica for one feeding. - **pH Stability**: Rhino Skin’s potassium silicate may slightly raise pH, but pH Perfect Bloom should counteract this. Verify pH after mixing (5.5–6.5 for hydro, 6.0–6.5 for soil). - **Redundancy**: Betaine, proline, and silica all enhance stress tolerance (osmotic, metabolic, structural). In optimal conditions (e.g., indoor, 70–80°F, 40–60% humidity), silica’s benefits may be marginal, but under stress (e.g., heat, pests), it adds a complementary layer of protection. - **Flushing**: Stop all additives (silica, betaine, proline) 1–2 weeks before harvest, using plain water or Advanced Nutrients Flawless Finish to avoid residual taste in buds. - **Cost vs. Benefit**: Rhino Skin is relatively affordable, but its benefits may be less pronounced in well-managed grows. Test on a few plants to assess impact. - **Cannabinoid Effects**: No evidence suggests silica alters THC, CBD, or terpenes, but improved plant health could enhance resin production. Overuse risks silica deposits affecting flavor if not flushed. ### **Summary of Effects** Adding **0.5 mL Advanced Nutrients Rhino Skin** to a 2-liter solution with **Bloom (2–4 mL)**, **Bloombastic (1 mL)**, **betaine (250–500 mg)**, and **proline (230–460 mg)** could: - Strengthen stems and leaves, supporting heavier buds. - Enhance resistance to abiotic (drought, heat) and biotic (pests, fungi) stresses. - Improve photosynthetic efficiency and nutrient uptake, complementing betaine and proline’s osmotic and metabolic benefits. - Potentially increase yield (28–112 g/plant) by reducing stress setbacks. The 0.5 mL dose (0.25 mL/L) is safe for autoflowers, keeping EC within 1.2–1.8 mS/cm. Apply during vegetative to mid-flowering, stop 1–2 weeks before harvest, and monitor for nutrient burn. The synergy of silica (structural), betaine (osmotic), and proline (metabolic) could optimize autoflower health, especially under stress. If you share your grow setup (e.g., hydro/soil, indoor/outdoor, strain, growth stage), I can refine dosing or application further. Let me know if you need sourcing tips or additional guidance!