|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 26 Summary - Ensign's log, stardate 17608.3, Wow what a week! Drying is always pretty simple and been checking daily. Buds are drying nicely and terps are smelling pretty dank!! Most of the week has been in the veg tent with tissue culture! Mixed another batch of establishment media and took proper explant cuttings. Also super-cropped the mothers to even out the canopy. Ahh yea! Getting hyped for trim day next week! Next week will also be the last week for this grow log, but am already planning out the next cycle. Anyways! Onwards to next week! [START OF WEEK 26] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk19] - WEEK 26 - Dry Wk1 ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 09 - Full Veg Gorilla Goat #1 - WEEK 05 - Full Veg Heady OG BX1 - WEEK 09 - Full Veg Garlic Budder OG#1 - WEEK 05 - Full Veg 10/13/25 → 10/19/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~0 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.5 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 00:00 - 00:00 | Wattage: ~000.0W [LV00] Distance From Canopy: ~00” DLI: ~0.00 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.17 kPa Soil %: [47.4, 34.1, 35.0, 55.7, 43.7, 43.6, 44.0, 43.3] ========================================================== Day 01 - Monday - 10/13/25 - Total Days: 183 ----------------------------------- Daily Averages ~[+01:00] - Temps: 78.8°F [26.0°C] - Humidity: 64.4% - VPD: 1.19 kPa - Soil %: [49, 34, 35, 57, 44, 44, 44, 42] ----------------------------------- - [22:05]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great! --- Heady OG BX1 #1 is drying along and no signs of mold. Also airflow and RH is stable and within the 60-70% range. --- Getting stoked! Trim day is 9 days away! - [22:09]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 40% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 40% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [22:18]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- All of the vegging mothers are looking great and the canopy is starting to even out. --- Also clones look healthy and no need to water yet. --- The stems, err, I mean, explants, don’t show any signs of contamination. --- Staying positive, glad the growth media is sterile and the overall procedure was successful. --- Anyways! Plan to create more establishment media and cut proper explants soon! - [22:21]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 10/14/25 - Total Days: 184 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.9°F [24.9°C] - Humidity: 64.8% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 43, 44, 43] ----------------------------------- - [21:44]: Night check on the 4x4 tent. --- Looking good in the dry tent! --- Heady OG is drying along and humidity levels look great! --- Nothing to do but maintain and wait to trim in 8 days! - [21:55]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 41% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 46% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [21:59]: Checked the 3x3 veg tent. --- Looking healthy and growth is raging! --- All mothers are looking beautiful! --- Clones are getting taller and looking healthy! --- Gorilla Goat #2 needs watering. Let’s go Ensign! - [22:03]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 4 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 230.8g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [22:23]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.7g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. --- Decided to train and super-crop both clones. --- Bent the taller branches to lower the canopy. - [22:38]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Onwards Ensign! Day 03 - Wednesday - 10/15/25 - Total Days: 185 ----------------------------------- Daily Averages ~[+01:00] - Temps: 75.6°F [24.2°C] - Humidity: 63.8% - VPD: 1.09 kPa - Soil %: [48, 34, 35, 56, 44, 43, 44, 42] ----------------------------------- - [+01:33]: Quick night check on the 4x4 tent. --- Looking great in the dry tent! --- Also starting to smell dank! --- 7 more days until the big trim! - [+01:37]: Checked the 3x3 veg tent. --- Veg garden is healthy and growth is green! --- Both the Heady OG BX1 #1 and TKO mothers will need another topping soon. --- Clones in the propagation tray also look great and no need to water. --- The explant media is still looking sterile with no signs of contamination. --- Easy night in the garden. Nice! - [+01:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Okay! Tomorrow plan to dive back into tissue culture and prep more growth media. --- Onwards Ensign! Day 04 - Thursday - 10/16/25 - Total Days: 186 ----------------------------------- Daily Averages ~[+01:00] - Temps: 74.6°F [23.7°C] - Humidity: 62.2% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 44, 44, 45] ----------------------------------- - [11:41]: Alright! Back to tissue culture and time to start another batch of establishment media! --- Took the plant growth regulators out of cold-storage (my fridge). ------ [ » SMOKES A BOWL « ] --- While the products warmed up, gathered the necessary supplies and setup my workstations. --- Currently, focusing on: ------ PHASE 01 » ESTABLISHMENT --------- » DAY 01 » MEDIA - PART 01 - Mixing the growth media --------- » DAY 01 » MEDIA - PART 02 - Sterilization of media and tools [via autoclaving] --- Going to use the same establishment formula, but doubling the yield. --- Also using RO water instead of distilled water. --- Here's the establishment media formula: --- [» VER.0001 |» INITIAL TROPICAL PLANTS FORMULA |» 0.5L (500mL) RO WATER «] ------ MS [Muashige & Skoog] --------- » 2.22g ------ Sucrose [Cane Sugar] --------- » 15g ------ BAP [6-Benzlaminopurine | Cytokinin] --------- » 0.5mg » 0.5mL » 500μL ------ IBA [Indole-3-Butyric Acid | Auxin] --------- » 0.05mg » 0.05mL » 50μL ------ AGAR [Jelling Agent] --------- » 3.5g ------ PH [Potential Hydrogen] --------- » 5.7 --- [» 1mg/mL CONCENTRATION ratio | 1mg = 1mL | 1mL = 1000μL «] - [12:59]: Time to prep the growth media! --- Made sure to turn OFF the lung-room's oscillating fan. --- Filled a measuring cup with 400mL of RO water. --- Dropped a clean magnetic mixing bar into the measuring cup. - [13:05]: Placed the measuring cup on the magnetic stirrer. --- Turned the unit ON and have the unit on LOW. - [13:11]: Using a precision scale (sensitive to 0.01g) along with a micro-pipette (1000μL capacity along with tips) and disposable (100mL) weigh boats, ------ Added 2.22g of MS [Muashige & Skoog] ------ Added 15g of Sucrose [Cane Sugar] ------ Added 500μL of BAP [6-Benzlaminopurine | Cytokinin] ------ Added 50μL of IBA [Indole-3-Butyric Acid | Auxin] ------ Added 3.5g of AGAR [Jelling Agent] ------ Then filled the measuring cup to the 500mL line before covering the top with plastic wrap. --- Alright! - [13:46]: Let the media mix on the magnetic stirrer for a bit. --- Have the unit set to LOW with a steady vortex. ------ [ » ~20 MINUTES LATER « ] - [14:09]: Okay! Time to pH the establishment media! --- Gently removed the bottom cap of the AC Infinity PH Meter PRO [AC-PHM7]. Placed the cap in the storage case for later. Covered the top with plastic wrap (KCL solution still inside the cap). --- Swirled the probe in a cup of RO water to rinse off the storage solution. --- Shook the excess water out and wiped the unit with a clean paper towel making sure all mineral buildup was removed (kept away from the very tip of the probe). --- Temporarily placed the meter back into the cup of RO water. --- Removed the plastic wrap of the establishment media. - [14:13]: Using the AC Infinity PH Meter PRO [AC-PHM7], --- Took the establishment media's initial pH reading. --- The pH was at 5.14 --- Using the micro pipette, ------ Added pH Up solution. --- Made sure to let the media solution mix for a bit before checking for pH readings. - [14:21]: Adjusted the media's pH until it stabilized to 5.58 --- For this batch, tried to go for a range of 5.4 to 5.8 pH. --- Also glad that I did not need to add PH down. - [14:43]: Poured the pH'd establishment media into separate polypropylene containers. --- Made sure to re-mix the media solution between container fillings. --- Ended up splitting the media solution into 12 containers. ------ 8x » 125mL Polypropylene Bottles [with screw cap lids] ------ 4x » 236mL (0.5pt) Glass Mason Jars [with polypropylene jamming lids] --- Have the lids of all vessels loosely on and set each aside for later autoclaving. --- Also filled 12 empty polypropylene bottles with RO water and placed the lids on loosely (4 bottles are re-used). - [14:58]: Prepped the tools for sterilization. --- Took a new 5.25in x 10in sterilization pouch and added the following tools: ------ 3x stainless steel forceps ------ 3x stainless steel scissors --- Before placing into the pouch, wiped each tool with a paper towel sprayed with 70% isopropyl alcohol. --- Sealed the bag and marked the date. --- Time to autoclave everything in the pressure cooker! --- Filled a Presto 23qt-Pressure Cooker with 12 cups of RO water. - [15:06]: Added a few aluminum foil balls to the bottom of the pressure cooker. --- Placed the first rack on top of the foil. --- Made sure the rack was level and stable resting above the water. --- Gently placed the containers of media into the pressure cooker and onto the first rack. Lids are still loose. --- Made sure to give space for each container and kept them away from the sides of the pressure cooker. --- Placed a second pressure cooker rack on top of the containers of media. --- Placed the containers of RO water onto the second rack. Lids are still loose. --- Placed the third rack on top of the containers of water. - [15:16]: Lastly, placed the sterilization bag of tools on top of the third rack. --- Took the pressure cooker lid and made sure the release vent was clear of debris before placing it on. --- Sealed and secured the lid. --- Pressure vessel has been sealed! --- Alright! Time to sterilize! --- While keeping the vessel level and straight up, moved the sealed pressure cooker to the kitchen for heating. - [15:23]: Placed the pressure cooker onto a butane-gas stove. --- Turned the stove ON and set it to HIGH. ------ [ » ~20 MINUTES LATER « ] --- Once steam was venting out of the release vent, --- Placed the metal rocker onto the vent. --- Shortly afterwards, the pressure top-lock sprung up to indicate the vessel was under pressure. --- Then the pressure gauge started to rise. ------ [ » ~10 MINUTES LATER « ] --- At 15psi, the metal rocker started to rock! - [15:56]: Turned down the stove down to LOW. --- Set a timer for 15 minutes and began monitoring the pressure gauge. --- Maintained the 15psi by making minor adjustments to the stove. ------ [ » ~15 MINUTES LATER « ] - [16:12]: Turned the stove completely OFF and waited until the pressure cooker's top-lock dropped down which indicated that pressure levels were safe to open. ------ [ » ~40 MINUTES LATER « ] --- Once the pressure cooker was at safe levels (0psi) and the top-lock was down, - [16:48]: Moved the pressure cooker into the lung room. --- The pressure cooker was still quite hot so was careful to keep the vessel straight up and level during transport. --- Used towels for insulation. --- Rested the hot pressure cooker onto a stack of 5-gallon buckets covered with layers of towels and folded paper bags. --- Gently opened the pressure cooker and set the lid aside. - [16:57]: While wearing nylon gloves, sprayed my hands with 70% isopropyl alcohol. --- Gently removed the bag of sterile tools placed it onto a Pyrex dish. --- Removed the top rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle of sterile water before placing onto the Pyrex dish. --- Removed the middle rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle and jar of sterile media before placing onto the Pyrex dish. --- Throughout the entire process, kept the gloves on and sprayed my hands regularly with 70% isopropyl alcohol. --- Decided to store all the bottles in the Pyrex dish. - [17:16]: Have the dish resting on a shelf in the lung room. The jars of media are set right next to the Pyrex dish on a towel. --- To prevent dust, covered everything with towels. --- Done for now and will check on the growth media tomorrow. --- If all goes well, should be taking new explant cuttings in a couple days! - [17:39]: Night check on the 3x3 veg tent. --- Looking beautiful and healthy in the veg garden! --- All moms are getting bushy and getting pretty close to the grow light. --- Since I plan to take explant cuttings soon, not going to top or train quite yet. --- Clones also looking healthy and seem unaffected by the super-cropping. Nice! - [17:57]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 45% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 44% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [17:59]: Checked the 4x4 main tent. --- Ca-Caw!! I got Headies! --- Wow! Heady OG BX1 #1 is drying along beautifully and she is smelling dank! --- Also no signs of mold and RH levels look stable! --- 6 more days until the big trim! - [18:11]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Still gots more to do! --- Let’s go! Let’s grow! --- Onwards Ensign! Day 05 - Friday - 10/17/25 - Total Days: 187 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.0°F [24.4°C] - Humidity: 62.0% - VPD: 1.16 kPa - Soil %: [48, 34, 35, 55, 44, 44, 44, 42] ----------------------------------- - [22:57]: Night check on the 4x4 tent. --- Ca-Caw! Headies is smelling dank! --- RH levels are getting a bit dry, but are okay. --- Wow buds are frosty with trichomes! --- With 5 days until trim day, getting stoked! - [23:04]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 47% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:11]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- Mothers are getting bushy and tall! --- Super-cropped and trained the taller branches down to lower the canopy. --- Plan to take tissue culture cuttings tomorrow so glad there are plenty of tops to choose. Still deciding on which mother to preserve next. --- Clones in the propagation tray look healthy! --- The original Garlic Budder #2 clone needs watering. - [23:26]: Here is the starting initial watering range: ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Garlic Budder OG#2 » 258.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:40]: Took the saturated weight after watering, ------ Garlic Budder OG#2 » [000.0]g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [23:45]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 10/18/25 - Total Days: 188 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.8°F [24.9°C] - Humidity: 60.3% - VPD: 1.25 kPa - Soil %: [47, 34, 35, 55, 44, 44, 44, 47] ----------------------------------- - [15:19]: Decided to decrease the VPD level a bit. --- Within the AC Infinity App, --- Changed the schedule name from 'Flower [12/12]' to 'Drying [0/24].' Oops. --- For the Main Tent [4x4] controller, --- Adjusted the 'Drying [0/24]' Schedule for the exhaust fan. ------ Decreased the VPD LOW trigger value from 1.5kPa to 1.4kPa --- Adjusted the 'Drying [0/24]' Schedule for the humidifier. ------ Decreased the VPD target value from 1.5kPa to 1.4kPa --- This should increase the relative humidity a bit and will maintain a 60-65% range - [16:59]: Evening check on the 3x3 veg tent. --- Wow the veg garden is raging with healthy growth! --- Even with the lower veg temps, growth has been perky and bushy! --- The TKO mother has been looking a bit on the purple-side, but I plan to increase the temps back after trim day. - [17:23]: Okay! Time to continue the tissue culture journey! --- Checked on the sterilized media, water, and tools. --- Ooo yea! The growth media in the glass jars have solidified nicely! --- The media in the bottles also look great! Found only one that did not gel properly. --- Hmm. The sterile water bottles are not looking sterile. Unlike the first batch (using distilled water), each RO bottle had tiny white particles floating in the water. --- I think this was due to the water source. The RO water used was from the plant reservoir. For the next sterilization, will try using water straight from the tap. Will discard the entire batch and try again later. - [17:34]: Anyways! Time to cut and sterilize another batch of explant cuttings. --- Using the similar protocols as last time, but with a few changes. --- Instead of distilled, using reverse-osmosis (RO) water. --- Also simplifying the workflow and will not be using the sterile water. --- Only taking cuttings from one cultivar. --- Today, attempting to preserve Heady OG BX1 #1 --- Unlike the last attempt, going to be cutting apical nodes and will leave all leaf growth on. --- Last attempt was more for monsteras and not cannabis. - [17:35]: Made sure the lung room's oscillating fan was OFF during the entire process. --- Put on a fresh pair of nitrile gloves. --- Okay! Let's grow Ensign! - [17:47]: Filled a glass beaker with 180mL of RO water. --- Using a micro-pipette, ------ Added 20mL of Clorox bleach --- Mixed the solution. - [17:52]: Poured the bleach solution into a glass mason jar. ------ Added 1 drop of Dawn dish soap. --- Dropped a magnetic mixing rod into the jar before setting it onto the magnetic stirrer. --- Turned the unit ON and set it to a low setting. --- Placed a polypropylene lid loosely on. - [18:02]: Rinsed and filled the glass beaker with 70% isopropyl alcohol. --- Filled another glass beaker with RO water before adding a drop of Dawn dish soap. Mixed the solution. --- Okay! Time to take the cuttings! --- Using a clean pair of trimmers, - [18:09]: Gently cut two clone cuttings from the Heady OG BX1 #1 mother. --- Stripped each cutting down to 2-inch apical nodes making sure to leave about 0.5 inch of stem growth for planting. ------ Prepared a total of 2 nodal cuttings. --- Dropped the nodal cuttings into the cup of soapy water. --- Swirled the cuttings in the soapy water for a bit. --- Opened the jar containing the 10% bleach solution. --- Using clean tweezers, --- Took each cutting out of the RO water and carefully submerged it into the beaker of 70% isopropyl alcohol for about 30 seconds. --- Then transferred the cutting from the isopropyl alcohol straight into the jar of bleach solution. --- Once both cuttings were in the jar, sealed the top with the polypropylene lid. - [18:23]: Flipped and set the entire bleach jar upside down onto the magnetic stirrer. --- Started using the magnetic stirrer. --- But the cuttings were getting hit by the bar so ended up turning the unit OFF. --- Instead, made sure to swirled the bottle every few minutes to agitate the cuttings. --- For a proper sterilization, the cuttings should be agitated in the hypochlorite (bleach) solution for at least 20 minutes. --- While waiting for the explants to sterilize, prepped the still air box. - [18:39]: Unfolded and placed a Safloga still-air box onto my desk. --- Thoroughly misted the inside of the air box with 70% isopropyl alcohol making sure to coat the entire chamber. --- Then isopropyl misted and placed the following items inside of the still air box, ------ x1 » Jar of Establishment Media ------ x1 » Bottle of Establishment Media ------ x1 » Glass-Bead Sterilizer ------ x1 » Roll of Plastic Wrap [pre-cut] ------ x1 » Sterilization Bag Containing: --------- x3 » Stainless Steel Forceps --------- x3 » Stainless Steel Scissors --- Made sure to fully coat the outside of the tool bag and each of the containers with 70% alcohol. --- The bag had a torn corner (Oops), so made sure to spray the inside with a few mists of isopropyl alcohol. --- For the glass-bead sterilizer, ------ Wiped the entire unit with isopropyl alcohol. ------ Before filling the unit with the included beads, misted the heating chamber with a spray of isopropyl alcohol. ------ Misted the glass beads again with a spray of isopropyl alcohol. --- Set the cleaned glass-bead sterilizer into the still air box. - [19:02]: Still air box fully prepped for explants! --- Okay! Explant nodal sterilization complete! --- While still wearing gloves, --- Misted the outside of the explant jar with 70% isopropyl alcohol before placing it into the still-air box. --- Turned the glass-bead sterilizer ON. --- Made sure to re-seal the chamber. - [19:05]: Okay! Finally time to start the tissue culture transplant! --- Let's go Ensign! --- Sprayed my gloved hands with 70% isopropyl alcohol (also put on a pair of cloth arm sleeves), --- Placed my hands through the arm holes and into the still air box to begin the process. --- Started by laying out my initial workspace: ------ » Far Left Quadrant » Jar of Sterile Establishment Media ------ » Far Center » Bottle of Sterile Establishment Media ------ » Far Right Quadrant » Glass-Bead Sterilizer ------ » Mid Center » Bleach Jar with Explant Cuttings [Main Workspace] ------ » Near Left Quadrant » Empty [Trash Area] ------ » Near Right Quadrant » Bag of Sterile Tools & Roll of Plastic Wrap --- Opened the bag of tools and placed the forceps and scissors into the pre-heated glass-bead sterilizer. --- Set the bag in the trash area (near left quadrant). --- Loosened the lids of both containers of sterile media but did not remove the lids. The lids were resting on top and could easily be lifted. --- Also took lid off of the bleach jar and gently set it on the table. --- Took the jar of sterile media and set it in the center of my workspace (to the right of the bleach jar). --- Using my left hand, took a set of scissors from the glass-bead sterilizer and gently cut the bottom of the first cutting. --- Once the bottom cut was made, placed the scissors back into the glass-bead sterilizer. --- Using my left hand, lifted the lid off of the jar of sterile media and inserted the trimmed end of the cutting straight into the growth media (using my right hand). --- Covered the jar with the lid and placed the forceps back into the glass-bead sterilizer. --- Tighten the lid of the jar and set it aside. ------ [ » REPEATED THE ABOVE PROCESS FOR THE BOTTLE OF GROWTH MEDIA « ] --- Once the last cutting was transferred from the bleach jar into the sterile media, --- Turned the glass-bead sterilizer OFF. --- Cleaned up my workplace and placed the scrap stem tips and bleach jar out of the way (near left quadrant). - [19:28]: Wrapped the lid of each explant container with plastic wrap before setting it in the center of the workspace. --- Explant transplant completed! - [19:59]: Using a label printer (Brother DT410), made a set of labels for each container: ------ Heady OG BX1 #1 | 04.24.25 | Photo | Reg ------ Formula: v0001 | ExBatch: 0002 | Lot: 01 | Pod: 01 ------ Establishment Media | Sealed On: 10/18/25 --- Opened the still air box. --- Then one container at a time, took an explant out and wrapped the lid with a label. --- Set the labeled containers onto the propagation tray (in the 3x3 veg tent). - [20:07]: Done! ------ The explants are around 37 μmol/m²/s which gives a DLI of 2.40 mol/m²/d under an 18/6 light cycle. --- Will check daily for contamination. --- Ca-Caw!! Ahh yea! --- Heady OG BX1 is now officially my first true attempt at cannabis tissue culture! --- Using this new workflow, I may try reusing the TKO media. Do plan to take new cuttings soon. May even try using the liquid media. --- Shout out to microclone and Bill Graham! His 'Tissueponics' video contains tips and tricks of the trade that have straight up changed the way I think of DIY cannabis tissue culture. --- Plan to further refine my process and will continue to refine the workflow with each attempt. - [20:15]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 54% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 48% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 45% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:56]: Checked the 4x4 tent. --- Ca-Caw! Looking great my ladies! --- Humidity levels are still a bit on the dry side, but still okay. - [21:08]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Oh yea, 4-more days until trim day!! Ca-Caw! Let’s go! Day 07 - Sunday - 10/19/25 - Total Days: 189 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.7°F [24.8°C] - Humidity: 59.4% - VPD: 1.27 kPa - Soil %: [48, 35, 35, 55, 44, 43, 44, 42] ----------------------------------- - [07:08]: Quick morning checked on the 3x3 veg tent. --- The veg garden is healthy and perky! --- All moms and clones are healthy and raging with growth! --- Both Heady OG BX1 #1 explants also look alive and green! - [19:26]: Night check on the 4x4 tent. --- Ca-Caw! We got MENDO DOPE in the trunk! --- Wow terps are getting loud and buds are drying along nicely! --- Looking frosty and coated in trichomes! --- Three more days until trim day!! - [19:33]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 46% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 43% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [19:35]: Checked the 3x3 veg tent. --- Vegging along beautifully in the garden! --- Mothers are getting taller and probably could train, but will wait until tomorrow. --- Clones and explants also look healthy and green! --- Gorilla Goat #2 needs watering! - [19:44]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 5 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 244.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [20:04]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.6g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [21:41]: With a few days before the big trim, decided to sample a few buds! --- Cut and trimmed branch for sampling and wow she’s got terps! --- Buds are dense and have a gassy diesel funk! Ca-Caw!! Wow! --- Without even curing, pretty sure she’s the one! --- Plan to grow out another cycle (in clone of the same pheno), but I think I found my female for my first breeding project! - [22:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Ahh yea! Three more days until the big trim! --- Also next week will be the last for this log. --- But already stoked for the next! --- Planning on popping more seeds and continuing the pheno hunt for another female! --- Since I lost TKO this harvest cycle, plan to grow a clone cutting of the same pheno (#2). --- As for seeds, popping more regulars and going to attempt to grow some of Mr. Grow-It’s Chillout OG! --- Got a pack of F3s and am stoked to give them a try! --- Also going to sprout some beans of Dead Ops OG from Zaza Genetics! --- Chillout OG, Dead Ops OG, and another round of TKO! Let’s go! Let’s grow! --- Hunters of the dank! Ca-Caw! --- Anyways! --- Onwards to next week! [END OF WEEK 26]

Day 46 for this pretty lady. She’s so big and bushy and full. I’m just in awe of how she turned out. Very very excited for her she’s full of life and vigor. Nothing I’ve thrown at her has seemed to matter. Idk what Russian means but hey who cares when they look like this. Hello beautiful. Can’t wait to see her full and bulk out.

fifth week flower, the ladies are getting really tasty and developing well!!

🌿🌿🌿🌿🌿🤩🤩

Day 73 ~ Plant 1 is looking great and hopefully will be ready to harvest by the end of the week. Been checking the trichomes and while most are getting milky white, there are still a couple sites on the plant where I find clear trichomes. Plant 2 seems to be about a week or two behind the first plant...I will give plant 2 their final Nutes tmrw and then begin to flush. From reading about this strain it seems like it should take around 90 days from seed to harvest. So I would think plant 1 is running a little ahead of schedule and plant 2 is right on time. Day 75 ~ Both plants looking and smelling amazing! I was hoping to be harvesting the first plant by this weekend, but she is still growing and her trichomes are still not all milky and I haven't yet to find an amber one. So it might be another week or so before I can start drying her out.

7/27 My phone ran out of storage and wouldn't let me take a video. I tried deleting several other videos bit that didn't work either. Finally I wad able to upload a few RANDOM pictures from the "website" camera. But no video. Everything looked so beautiful tjis morning too. Not watering was the right choice. I defoliated a couple septoria leaves on the reveg 10th planet that wad about to receive its third dose of Plant Doctor. The chem dog #4 and mk ultra this will be their second treatment. Seeing the positive impact of the product and finding a few septoria leaves I decided to treat every plant in the garden with plant doctor. I'm still going to do the diy Dr. Zymes but Plant Doctor had been working good and I want to suppress as much as possible. Everything looks great. I started by giving the thirstiest plants a half gallon of water. I then did a root drench of 3tsp per gallon on every plant but the 10gal. A gallon of solution was used on each plant. I really feel like this is a good mood. Previously AFTER I applied Plant Doctor the plants would have a noticeable positive effect shortly after. I'm hoping this will be the case here. I think stretch has stopped. I have one special Kush that has buds already but EVERYTHING is in early flower. I'll fix the phone and get a video up there. EDIT: WENT BACK AROUND ONE TO DO A LITTLE DEFOLIATION. EVERYTHING LOOKS REALLY GOOD TO ME. I DID DEFOLIATE A COUPLE SEPTORIA LEAVES. BUT REMOVING A LEAF OR TWO FROM A MONSTER REVEG IS NOTHING. REALISTICALLY I SHOULD BE REMOVING MORE FOR BETTER AIR FLOW. THATS SOMETHING ILL NEED TO DO. SO HAPPY THINGS ARE DOING GOOD. EVERYTHING IS IN VARIOUS STAGES OF FLOWER. NEXT FEED ILL BE USING A BLOOM NUTRIENT. NOT SURE BUT PROBABLY LIQUID KOOL BLOOM. 7/28 Didn't have much time this morning. I've got a wife with medical problems and I'm trying to redue my house before the end of the summer so I'm busy to say the least. Still no pm. Not many pests either. The rose bushes behind them have been DEMOLISHED by jpn beetles. I see no negative active reactions from treating everything with plant doctor 3tsp/gallon. The first plant I treated with it was that 10th planet and its gotten three treatments and looks great. I'll get a spot here or there once in a while but for the most part it's supressed. I have some pruning to do. Everything os in early flower but that one special kush is pretty far ahead. EDIT: SINCE IT WAS MID 80S I WENT OVER AROUND 1 TO CHECK THE GIRLS. THEY LOOKED GREAT. I DEFOLIATED A COUPLE SEPTORIA LEAVES BUT ONLY A COUPLE. I STOPPED AT A LOCAL SHOP AND SHOWED MY BUDDY A VIDEO. HE SAID TO NEVER SPRAY ANYTHING WITH LEAF SEPTORIA WITH "ANYTHING" BECAUSE OF HOW IT SPREADS. I DIDNT MENTION THE CONTRADICTORY EVIDENCE THAT I'VE FOUND IN MY RESEARCH BUT HEY. HE HASNT LED ME WRONG. PLANT DOCTOR SEEMS TO BE DOING THE JOB. THAT AND DEFOLIATING ANYTHING I SEE. IVE WORKED TOO HARD TO LOSE THEM TO A DISEASE THAT MAINLY EFFECTS THE LEAVES. PLANT DOCTOR WILL KEEP IT SUPPRESSED. 7/29 Today is supposed to be really hot. In the 90's. I watered everything a gallon except the thirstier plants got 1.5 and the 10's got 1/2 gallon. I defoliated a few septoria leaves. I'm sure there will be a few more after watering. Instead of alleviating my concerns my buddy kinda got in my head with the septoria talk. Things will be fine. Everything LOOKS gorgeous. He even said, "They look really good and healthy" and "not to get complacent defoliating." The plant in the 50 seems to be worse off than the others. I'll keep an eye on it but if I got spots near the sugar leaves I might just cut that little runt. This was all preventable too. Person mowing the lawn BLEW DISEASED GRASS, SHRUBS AND BIRD SEED IN MY CAGE RIGHT AFTER TRANSPLANT. I legit had to dig out at least a dozen sunflowers or other seeds that sprouted. Realistically things will be fine. The plant I've been treating for three weeks with plant doctor looks great. So do the others. Everything looks good. I just get occasional leaves I previously would've overlooked or attributed to something else. As long as I can keep it suppressed enough through flower I'm good. Anything that isn't good enough will go towards extracts. EDIT: WENT OVER AROUND ONE. A COUPLE PLANTS IN TJE BACK THAT I WATERED FASTER WERE LIGHT SO I SPLIT A GALLON WITH THEM. THE 10TH PLANET THAT HAS GOTTEN THE FULL PLANT DOCTOR TREATMENT IS DOING AWESOME. I DID HAVE TO DEFOLIATE A HANDFUL OF LEAVES. HONESTLY I THINK IVE PROBABLY HAD THIS YEARS PRIOR AND DIDNT RECOGNIZE IT. IM PRAYING EVERYTHING WORKS OUT. IM DOING MY PART. I'M GOING TO HAVE TO DO SOME MORE RESEARCH. 7/30 Came over to the 10th planet plant in the front row all the way in the back was drooped all down and is yellowing up pretty good. I defoliated what I could and gave it a gallon and a half of water. I defoliated any sep leaves I could find. If that plant foesnt pick up like they normally do I may need to ditch the plant. If it continues to get yellow and look unhealthy I'd rather get rid of it than risk more damage. It was light as a feather so I assume it will be fine. I'll update as I go. Everything else looked good though. EDIT: DESPITE IMMENSE ANXIETY I GOT THAT FEELING SO I WENT AND CHECKED THEM. THE ONE I WATERED DID PICK BACK UP BUT IT HAS A LOT OF YELLOW LEAVES. THE 10TH PLANET THATS DOING GOOD WAS BONE DRY AND STARTING TO DROOP. I GAVE IT 1.5 GALLONS. THE HUGE MK ULTRA ALSO WAS STARTING TO DROP AND LIGHT AS A FEATHER. I GAVE THAT A FULL WATERING CAN WHICH IS DAMN NEAR 2 GALLONS. THE REST WILL GET WATERED TOMORROW. NO SIGN OF SEP THIS TIME. I WASNT THERE LONG BUT STILL. THE OTHER PLANTS WERE LOOKING GOOD AND ALL PRAYING TO THE SUN. TOMORROW ILL WATER AND FEED. 7/31 Came over this morning to one of my plants on the 10 drooped right over. I shouldve watered it yesterday when I did the others that were light. Coincidentally the three that needed the water were the 3 revegs. I watered what didn't get it yesterday with a gallon each. I then fed everything a powerade bottle. Started liquid kool bloom today. We shall see how that goes. I defoliated quite a few leaves. I think I may have had sep in the past on some plants and just didn't notice. Plants still look good but I keep a good eye on them. And I defoliate a lot. I've taken my buddies advice and not sprayed anything. I don't really have any pests. A pillar here or there or a "A" cricket or jpn beetle. Of there is one four eyed plant bug but I can't seem to kill it. I've almost caught it a couple times. Anyway like usual I'll keep this updated. 7/30 Girls looked good this morning. I has to water the 10th planet in the front back and one of the 10s. Defoliated a few yellow leaves and picked off a couple sep leaves. It rained a little and I'm really surprised that I haven't seen MORE sep leaves. These girls are resilient. One more day and it's plant Dr day. That 10th planet that has had 3 treatments and will just receive its first "preventative dose" after the three big ones is doing amazing. That one special kush I the front is flowering much faster than anything else. I'm grateful for what I have. EDIT: WENT OVER TO SEE IF I NEEDED TO WATER AROUND 1PM. EVERYTHING LOOKED GOOD. THE FURTHEREST KUSH WAS A LITTLE LIGHT BUT IT CAN WAIT FOR TOMORROW. I DEFOLIATED ANY FADING LEAVES OR ANYTHING THAT EVEN LOOKED LIKE SEP. ALL PLANTS ARE IMPROVING AND LOOKING REALLY GOOD. MK ULTRA IS GOING FIRTHER INTO FLIWER TOO. FOUND ONE JPN BEETLE WHOCH I PROMPTLY KILLED. AROUND THE GOUSE THERE ARE ROSE BUSHES. LUCKILY. JPN BEETLES ARE ON TOP OF EACH OTHER FUCKING AND DESTROYING THE PLANT. IM VERY LUCKY DAD LETS ME KEEP THESE AS TRAP PLANTS. I WENT OUT AND PROBABLY COLLECTED 50 BY BRUSHING THEM IN SOAPY WATER. I HAD TO DO THAT 2 OR 3 TIMES A DAY ONE YEAR. IM SO GRATEFUL ROSES ARE THERE FAVORITE FOOD. PLANNING TO WATER TOMORROW AND I MAY APPLY PLANT DOCTOR A DAY EARLY. IM NOT SURE. IT WILL HAVE TO BE A DAY LATE OR A DAY EARLY. ILL PLAY IT BY EAR. I THINK THEY COULD WAIT REALISTICALLY. IM JOT SEEING MANY BAD LEAVES. KNOCK ON WOOD.

This strain is just incredible, perfect indica high with a sativa sidenote It grows strong and is quite resistent to mold. All plants were done after about 60 days of flowering. First time i used a tumble trimmer and it works perfectly. I didn't check PH or EC at all

Welcome to my Runtz Auto Diary sponsored by Zamneisa and Spider-Farmer co2 ran for whole fattening period. Days 1-21 early week 7 missing. HST"ed some of her.17th/may 7th/June Power Buds really helped build lots of tight stigmata. Sorry about missing wk 7 and half of 8. Colas are getting big loving the light. Developing lovely fade around the leafs the past 2 weeks. Drinks like a fish. 2L a day easy on her last 10-14 days. Doing really well. 7th/June foxtailing is happening as you can see. She's very sticky and is still pushing. There's nothing I can do about the heat. Nothing. All I can do is lower the light intensity that now. Its been set at 90%.⁰ Am dealing with crazy temps like 35c on ave can go up to 39c. Am finding it hard to keep the stomata vents going. Seedlings are pushing through. I do see signs of stress. Possible foxtailing coming. Not much I can do with the temp. Stomatas are been kept open with fan power. I've 4 running nonstop. 1 full Osc one and 3 others that blow parts of the plant but its more designed to keep the co2 in the middle of the tent. It's taken me 2 weeks to find thy optimal way to manipulate co2 movement. If you don't have the fans right. You are just wasting money. I've seen s 50-30% increase in rate of just seedling growth that I prove. From 55ish strains that 95% ran from 9-10 Days the rare long one. But, I'm seen seedlings turn into vegging plants in an average of 6 days. Some as early as 4.5-7.5 days. Most are 6. And then they're flying through veg too. HST & defoliation HST was done early pre flower the nodes actually split and fell over. Mended itself up. Tbh, there is no real benefit to HST'in autos. I've done it many times now. The nodes that were HST"ed didn't vary much if at all. The whole plants buds are hard. Foxtailing like a bitch. But comes with the environment growing indoors in summer with co2 with 35c temp on average, power buds was used as soon as I seen stigmata and until pre fower ended. SE5000 light used for co2 growing. Doing a great job. Helps to have a great sensor. This spectrum is great. Even with high temps that should have vents closed if using say 1 spectrum but, this light is able to keep the plants eating with high temps. Has gave all my plants great fade. Next wk. I'll higher the light and lower it and start focusing on the crop this light was issued for. But, its done a great job at finishing my crop. Thanks to my sponsor from Zamneisa. Hopefully i can do your strains better justice next time if there is. I'm happy with the bud, as its an auto that spent a lot of her life under the SF-1000 before the SE5000 2 wks ago like to thank "Bella" for that upgrade. That allowed me to finish a few of my girls while setting my flying youngsters at a distance. I love everything about it. Much appreciation to all who stopped by to view, like &/R comment. Look forward to seen your work. COUPONS Marshydro.eu/ww "ggs" 3% Zamneisa "GROWITGD" 20% ww Spiderfarmer.eu/ww "ggst" 3%

Ciao ragazzi e bentornati qui con me le gemelle al Limone🍋😉 Ci siamo! Fra un paio di giorni taglierò loro. I buds sono estremamente compatti, profumati e di un colore favoloso, come potete vedere nei ravvicinati delle apicali.😍 Ho eseguito un lavaggio delle radici massivo nel giorno 92 con ben 20 L di acqua ciascuna per pulire bene e poi dopo aver aspettato che asciugasse per bene, ho innaffiato loro nel giorno 96 con 1,5 L di acqua.💪 Nel giorno 98 ho eseguito anche una voluminosa defogliazione per dare concentrare le energie suo buds negli ultimi giorni.👍 Eh niente ragazzi! Anche per questa settimana è tutto. 👊 Grazie a tutti per aver guardato e restate sintonizzati per nuovi aggiornamenti.🙏 Buona settimana e felice crescita 🌱 🌱 🌱

Schwerer rückschlag: sciarid larvae (trauermückenlarven) haben die Hälfte der seedlings angefressen. Nur die Pötte, wo neue pflanzerde drin war, wurden verschont. Diese Mücken legen ihre Eier in feuchtes Substrat und die Larven fressen Keimlinge und junge wurzeltriebe... klassisch Pech gehabt.. tausche jetzt die Erde und setze das dritte mal an diese Saison. Das Zeitfenster für automatics ist noch groß genug 🙂 Aufgeben ist keine Option.

First 35 days of flower. Will slowly decrease the feedings for the next 4 weeks and then chop. Smells great with purple hues in the leaves and buds. Every seed has had different physical characteristics but they all seem to have a similar gassy smell.

She germinated and sprouted :) Thanks zamnesia for the allowing me to partipcate in another growing contest!

Don't have a lot of time these days to take pics so I'll sum things up. CO2 got outta control and the plants took tf off literally. The video is around middle of week 3, I had to flip at week 4 BC I literally ran out of room and I had to constantly defoliate.

Perked right up when I started base watering.

Hellow growers. Not quite sure whats going on but im now under the impression this isnt an auto as labelled. Lights have been on an 18/6 schedule but now i have set them to 12/12. I should be seeing pistils by now if it was an auto lol. I aint mad if it aint but i have veged a monster. Her stem has lots of girth her canopy is nice and even. Defoliated 22/11 Yay positive they are not automatic. So correcting info.

Tag 84: Die letzte Woche hat meiner Shogun aufgrund der Hitze nicht so gut getan. Ihre Blätter sind sind insgesamt recht hellgrün. Ich versuche es aktuell mit noch mehr Alg a mic, vielleicht giebt ihr das ein wenig mehr grün zurück. Tag 89: Um die Shogun noch etwas in Form zu bringen habe ich das scrogging Netz hochgeschoben. Sie scheint langsam weniger Wasser zu benötigen, auch die Blätter sind schon recht hell, was wohl an den heißen Tagen liegen mag. Ist nur so das es noch über 5 Wochen sind bis zur Ernte 😲

Defoliated it a few days before harvest so plant can focus more energy on buds. Smells very citrusy, very powerful. Will harvest her in the next few days to start drying process :)

hi all, friends of the good weed we are proceeding with the drying and then dry cutting as we usually do these beauties have gone over and have not been able to contain the feedings well, but nothing to worry as they have popped out of the really beautiful and cold buds stay up to date for the smoke