Operation Flower of the Jungle is a Go! Second week of flower and these ladies are stretching and blooming! A part from the stretch and flowers coming out, there hasn't been much change. Which said like that seems like it's nothing but there has been a huge flower production in the past week. Were gonna have some nice jungle nuggets. Upped the nutrients 1ml/L

Day 16-27/12/21 all looking good!!!! Day 20-31/12/21 still haven’t got space in other tent yet so hopefully can move half of them out this one next week!!!!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Wow she's grown 24cm this week. I'm getting excited about her! I think she's looking to be a fab plant. Doing well in her fabric pot.

End of week 22: Overal it was a good run with the Shamans from @DutchPassion_Official . Didn't had mayor problems besides having to harvest them earlyer. I really wanted to flower them another 2 weeks but the weather/climate didn't allow me to. Still the buds came out decent as you can see for an early harvest. I will cure those buds for some time in my pots before they get smoked and will update how much weight I got out of the two plants. As you can see I made the bigger buds smaller. There is no use for me to keep big buds for showing off. Big buds just have big long stems in it, harder to dry and to fill your cure pots with.

Wow she's grown a massive 34cm this week!!!! Monster growth!

I was wrong about my clones I thought that I had two clones of mimosa evo and two red hot cookies then I was thinking maybe it’s green pheno, but now with the strong smell I recognize the blue widow from zamnesia🤭 I also think 🤔 that the clone looks the same as the mother plant so it cannot be green phenotype 🤪💪💪💚

So i gave her last feeding of grow and bloom nutes at the end of week @1600ppm, Ph@ 6.5 and on the next ill only be giving ripen and drop the ph to 6.0 to fatten her up a bit more before i chop her. She's absolutely stinking up the place with some sweet gummy lemon gas coming thru. Think ill call this strain 🍋🍭Lemon lightning🍭🍋

Welcome to week 25 of my bubba slush diary Genetics by Greenhouse seed co. Welcome to my 2nd diary within diary. The first diary is already harvested. The second one has been in veg for about 20 weeks, while it was in veg, i trained it with lst and hst, now we are a in flowering for a couple of weeks. Third diary will come within this diary too, i took a monstercrop clone, it's revegging now, curious of the outcome, since this strain already has tight internodes on the branches. Buds still packing, strong smell...

Overall, plants continue to grow well and recover from LST. Continue to worry about under / over watering and whether leaf / root mold or bugs is causing spotting on leaves and wilting leaves from time to time. Have fought off white bug infestation and possible yellow leaf mold (maybe ph flux or root rot) in past weeks, but seems to be under control now with rarely a bug sighted and old rust spots on leaves at bottom of 1 or 2 plants being replaced by vibrant green growth above. Trying to water ever 3 days or so with approximately 1/2 gallon of water each plant with 1/4 recommended Fox Farm nutrients and root protector from ILGM phd at around 6.3. Tent is starting to smell abit skunky when opened. Tallest plant is around 12.5 (White LSD) and smallest is around 7" (White Widow #3). Avg plant is around 10" tall.

Day 15 🌱 everything is going well but I have accidentally decapitated N1, in reality I had topping already N3 but with N1 I broke without doing it on purpose. hope for the best. Day 16🌱 wow today N1 I see it well it does not seem to have accused the topping. everything seems to be going well for N3 too, he seems to be growing very strong. N2 is in excellent condition. Day 18 🌱 i am struggling a bit with "lst" but i guess because i started early. otherwise they seem to be growing well Day 20 🌱 I let them rest for two days. some training again today, they look fit and grow great. Day 21🌱 N1 and N3 grow great and look great. N2 grows well but has yellowed leaf tips as if I had given too much fertilizer, I think due to the pre-fertilized soil. Now I continue with water and I will check the drain ph Thanks for viewing. if you like my diary leave a like and a comment. come back to see me 🎂 good cultivation and good day💚🌱

For now I'm happy with the germination,Even the transplant in the organic coco hydro growth modules all right yesterday✌️🏿

Budding like crazy under the new light,getting frosty. Plant 2 now catching up a bit,no purps though 😡 dropped temps. Around 20 days since first pistils No issues 🤞🏴󠁧󠁢󠁷󠁬󠁳󠁿🍁 🍁🍁🍁🍁 Breeder-White label purple bud auto 🍁🍁🍁🍁 Light-Voost VST 240(discount code TightNugs) https://voost.com/

02/18/2020 Look at those fat leaves, definitely does well in cool temps, ruderalis / indica genes. I can't wait to see her sativa wild side.🙏 turned bloom channel to 100% so blue and red spectrum at 100% full spectrum ir and uv included. Will do more pics latter this week. 02/19/2020 Day #21 and I think that she likes the red spectrum she just looks bigger than yesterday I think she got 2weeks of veg cycle, hope to top midweek,and LST.(if she got her 4th node,that is) Ps. Sorry about the red looking pics, I will get some in natural light soon as I can.🙏could not get in natural light but mh light looks good.

Germination date 🌰 10/03/2021 Day 71 🌱 18/05/2021 Strain 🍁 Purple Matcha, Humboldt seed bank Nutrients 💉 Advanced nutrients PH perfect sensi grow A+B (veg) PH perfect sensi bloom A+b (flower) B-52 (through veg until week2 of flower) Voodoo juice (🖕🏻) Tarantula (🖕🏻) Piranha (🖕🏻) Sensizym (all the way through) Rhino skin (🖕🏻) add first leave for an hour Big bud coco (week2+ of flower Bud xfactor (🖕🏻) Nirvana (🖕🏻) Bud igniter (first 2weeks of flower) Overdrive (last 2weeks of flower) Flawless finish (flush week) RockHoldings Rockresinator(week2+ of flower) Vitalink calmag Set Up ⛺ amazon special 1.2m x1.2m 💡 spiderfarmer sf4000 📤📥 AC infinity 6inch 💧 10lt dehumidifier Notes🗒️✏️ Final week of full nutrients done, will start to lower e.c this week and add overdrive. Apart from the slighty burnt hairs she looks 👀 and smells 👃 amazing. It smells alot like fruit loops the cereal, very sweet. Buds aren't massive on this strain but their rock solid and beautiful to look at. I can see this being a easy strain to chop up when its done and I cant wait to do it again. %100 recomend this but not for a newbie and if you struggle with over heating 27+. Will do this in the winter to gets it full potential. Thanks for following and liking means alot ❤️👍🏻 Sorry for the shit pics this week, phones dying slowly. Took some more and deleted the rest. New phone ordered Follow PharmaZ his account is bad ass and very handy for tips and tricks. Worth a follow guys and girls 🍑 Stay turned and happy growing fam ❤️🍁🌱👍🏻 New video ❄️❄️❄️❄️❄️❄️❄️ cant wait to bury my head in this strain when its done

Week 4 the girls just stacked weight

I'd like to say a big hello to all and thanks for taking a look at my first grow! all feedback is appreciated and I am happy to experience growing this plant even if I do not succeed this time. Apologies for the bad picture quality, I will improve this! without further ado lets give this Dwarf a happy life! Pre soaked the canna pro plus coco with plant start at 2ml/1ltr No activity from the plant in the first three days. using a big baggie as a humidity dome which has helped keep humidity high for the 🌱 Noticed on day 5 that the root has made its way through the soil bigtime and was desperately reaching for the light... immediately placed under the phlizon 3000w (630w true power draw) COB LED using only the LED supplemental lights (270w)and started a 2ml/ltr dose of plant start Day 6 - Plant has started to recover from being in the dark too long, starting rhizotonic today also at 2ml/l alongside 2ml/l vitalink plantstart. Regularly misting the humidity dome to keep humidity as near 70% as possible (sometimes with rhizotonic spray) Temperature is between 23c and 27c throughout the day. Day 7 - Nothing much to report other than healthy looking seedling, I've been alternating between the COB (330w) and LED (270w) lights throughout the day, have not to wanted to put them both on together incase I fry her :D Tomorrow I'm starting my week 2 and im seriously considering transplanting to my final pot (smart pot) which is a 5gallon/19liter pot... (will seek advice below) and switching from 2ml/ltr vitalink plantstart 2ml rhizotonic schedule to canna A +B 2ml/ltr canna rhizotonic 2ml/ltr.