Just as difficult to grow as the first time I done Afghan kush but still managed to get some nice purple bud out of it!!!

A hub of activity in the Fastbuds closet. All lower branches tied down, defoliation. Hit them with Shogun Geisha foliar a few days ago, and an application of Athena Stack ( Foliar) a day later. As far as I can see, they are thriving. Got another two (orange sherbet/gorilla cookies) which are 3-4 weeks younger. Germinated in soil, a first for me. Leaving the gorilla to do its thing, maybe defoliation/lollipop. The Sherbet is being put through a true mainline. The Shogun line is surprising me, I’m used to dry amendments/organics but it’s been so easy to follow their feed chart calculator on the website. Signs of pre flower showing on all of them , I’m expecting the stretch soon.

7/20 Went over this morning to plants soaking wet and heavy rain. Went back around 4pm and plants actually look really good. The rain has stopped for a bit and the girls looked great. Apart from some septoria leaves I needed to defoliate on the first plant I treated. I also noticed a few others on other plants so it's spreading albeit slowly. I treated the three in the middle with their second dose of Plant Doctor at 3tsp/1gal. One gallon administered to each plant via root drench. I think I may treat the other plant showing signs with a diy Dr. Zymes using citric acid as the active ingredient. That way I Gould start treatment right away without having to rely fully on plant doctor. Plus it would kill any pests. Ive seen damage but the birds do a number on them. I dont want to spray my plants with a bunch of chemicals if I don't have too. I shouldve replaced the pallets and cleaned the cage better. I think I did a good job sanitizing as I have no wpm. Just this septoria I probably got when the lady mowing the lawn cut all tjis fucking grass and blew it in my pen, throwing bird seed and I'm sure tons of disease into my grow bags. I literally had to harvest a bunch if fucking sunflowers. It looked like my plants had been mulched. Just with small disease carrying shrubs. At some point some of this stuff has to be on purpose. You CAN'T be that stupid. A couple kushes in the back look hungry. I think I may need to up my feed. I've written to much. I'll keep this updated. Thanks if you made it this far. 7/21 I guess it rained last night. Oh well. It was blue skies for hours before dark. It must've rained late/early morning. I'm sure the app of plant doctor will be fine. Like I said I'm considering using citric acid as a foliar spray to help with the septoria and clean up any pests. I don't have enough pests to warrant spraying. I just don't want to put all my eggs in the plant doctor basket. Today is overcast and windy. The wind has really assisted in spreading this virus. I remove anything I see infected immediately and since these plants are reveges they have plenty of leaves. Plants are really stretching. I know I talk about the septoria a lot but realistically if someone looked through my garden they'd have a hard time spotting it. I don't want to exaggerate the problem but I don't like dealing with ANY fungus or molds. 7/22 It was 55° this morning when I went over. I defoliated some septoria leaves and a few that were fading or damaged. I saw almost a whole leaf eaten so it's probably a good idea to move forward with citric acid. I can add that to the plants I've already treated with plant doctor. This is the first year it's been so noticeable. It's the reveg 10th planet that started this off. Oh well. I've dealt with MUCH worse. Plants LOOK good (other than septoria leaves (on bottom of plant). Today is water day for me but it's been raining and it's a lot cooler today. Bags still had weight to them. I want them to have a proper wet dry cycle. Temps top out at 75° so I'll monitor during the day and if something NEEDS WATER I'll give it to it. I'm suprised I have ZERO WPM. The septoria is much more identifiable but pm is usual present in these conditions bur I don't have a spot of it. I may do some defoliation today. I also need to add supports for flowering but one thing at a time. EDIT: Went over around noon. 70°. Bags seem to be holding weight (it was easier to tell as ONE plant was substantially lighter than the rest) so I decided to hold off on watering. At least until tonight. There are three plants (at least that are going to NEED water). They look phenomenal now though and we've had lots of rain. Plan is to go over later and get the light ones and mix the water for the others so I can administer it in the morning quickly. I have an early doctor's appointment but I don't to overwater or water unnecessarily just because. But when you grow alone sometimes you need to do things when your able. I have people there that would help if I asked. And I have before but its late . I'm very grateful. I defoliated quite a few septoria leaves. They were all from the bottom of the plant. A couole wete bottom interior. I know there are worse things to contend with but still a pain. EDIT #2 WENT BACK AT FOUR AND PLANTS LOOKED FANTASTIC AND STILL HELD WEIGHT. THE REVEG MK ULTRA IN EARLY FLOWER "MAY" HAVE STARTED TO DROOP AND "SEEMED" LIGHT. THE LARGE SPECIAL KUSH FUETHEST IN FLOWER BY THE DOOR WAS ALSO EXRRENELY LIGHT. I HAVE AN EARLY DOCTORS APPOINTMENT BUT DONT WANT TO OVER WATER MY PLANTS JUST BECAUSE IM IN A HURRY. ITS COOLER THIS WEEK. 70 TODAY AND HIGH OF 80 TOMORROW BUT DAD SAID THATS NOT UNTIL THE AFTERNOON. I MIXED UP ENOUGH WATER TO WATER EVERYTHING IN THE MORNING IF IT NEEDS IT. I HAVE AN IMPORTANT DOCTORS APPOINTMENT EARLY THAT WILL TAKE ME AWAY AND IM FEELING A LITTLE ILL SO I WANTED TO GET DONE WHAT I COULD TODAY. WATERED THE TWO THAT NEEDED IT WITH TWO GALLONS A PIECE (30GAL REVEG MK ULTRA AND THE BIG SPECIAL KUSH IN FRONT I DIDNT TOP THATS FLOWERING THE FASTEST. EVERYTHING ELSE CAN WAIT. 7/24 Surprisingly the plants still held weight this morning! The looked pretty good too! However its going to be highs in the 80's for the next 7 days. Since the plants still had some heft to them I watered everything just a gallon. I gave the 10s a half gallon and skipped the 50gal container plant. I think this is a good happy medium. I'm still seeing random septoria leaves but still no WPM. I defoliate daily sometimes a couple times. I need to get a minute to myself and add a foliar spray. I'll start with a dih Dr. Zymes while I'm looking into other products like bicilus (i have bt but its a different bicilus) or trichodermia. I need to feed sometime this week too.

Day 64 - the hulk is here! a. this plant has really surprised me and exploded everywhere b. Pumped out the DWC and installed 15 litres of my explosion flower blend. Adding liquid KoolBloom and keeping the Diamond Nectar flowing for this lady c. Ive noticed that continuing Diamond Nectar throughout flowering in strains that contain OG Kush make the buds much more dense. Day 65 - flowering really well a. I think she loves the new flowering blend! she drank 3 litres overnight! b. Im going to push this plant very hard to the finish c. keeping the PH 6.5 throughout all of flowering Day 66 - The sative genes are shining through! a. I wanted a super sativa plant for this grow, I want a mind numbing head high, this will do it b. it looks and smells alot like White Widow, probably due to the Myrcene terpenes. c. she is drinking 3-4L a day right now, Im dumping in the above chart mixed 1:1 right now. d. tied the larger side branches back a little to open her up, she is thick Day 67 - a little more guidance for the branches a. added in 2 ties for the large side branches to give space, there are many tops here b. she is drinking so much, it is crazy! c. the resin is already being produced, and is very sticky and strong Day 68 - explosions everywhere a. the buds are expanding vigorously, the stigmas are huge and very strong b. she is still stretching upward its amazing to see c. the plant is consuming 3-4L a day right now Day 69 - continue to grow a. drinking alot, 4L overnight, stretching like a giant b. lowered the light just a ilttle to get some more penetration into the plant Day 70 - growing like a weed!

Received TWO 5packs of rhino Ryder as freebies. Gonna grow these outdoors and see how they do. Gonna take advantage of socal sunshine. Only 4 out 5 sprouted. It’s old beans I think. So that’s a good pop rate. I think.

Devil cream Auto is oozing with crystals it's roots aren't booming out of the soil like a couple other plants in this run, however it is looking good.

Oh us baby!! Doing great!!

Absolut super wuchsform. Jab jetzt gehts in die breite. Cuts from roots farms at - best in austria and europe

🌱🌱🌱Continuamos este cultivo, con esta hermosa cepa de FastBuds, Girl Scout Cookies Auto, entrando en el periodo de crecimiento mas exuberante vegetativo.🌱🌱🌱 👨‍🍳👨‍🌾🏻 Nutrición y Bitácora 👨‍🌾🏻👨‍🍳 Día 15 se riega con una solución nutritiva NPK de 1 Litro con Plagron Power Roots (0.3 ml), Plagron Pure Zym (0.5 ml), Plagron Alga Grow (0.7 ml) y Plagron Sugar Royal (0.3). Día 16, se mantiene saludable creciendo 👨‍🌾🏻, asimilando de muy buena manera el riego nutritivo del día 15 Día 17, sigue creciendo y creciendo y cada vez mas hermosa esta bella planta 😍 Día 18. Se ajusta el LST para bajar el apical. Se riega con una solución nutritiva NPK de 1 Litro con Plagron Power Roots (0.3 ml), Plagron Pure Zym (0.5 ml), Plagron Alga Grow (0.7 ml) y Plagron Sugar Royal (0.3). También se ve la producción de mas ramas bajas que comienzan a tomar fuerza como posibles colas principales y también se ve la aparición de los primeros pistilos, así que estamos prontos a comenzar la Pre Floración, pero aun le queda un buen tiempo en su etapa vegetativa de máximo crecimiento. Día 19 sin novedades. Día 20 se instala la malla para direccionar las ramas. Se realiza defoliación para mejorar la penetracion lumínica y se riega con 1 litro de solución NPK + Trichodermas. Día 21 la malla mejora el uso del espacio, mejora considerablemente la penetracion lumínica y el crecimiento sigue de manera constante. La planta ya esta pidiendo mas alimentación y riegos, en 24 horas el sustrato se encuentra casi seco. Va de maravilla entrando a la cuarta semana 💪 🚀Equipamiento🚀 Indoor de 60x60x159 cm y una iluminación BlackCob F320, se activa solamente 1 modulo (160w) a 70cm para estimular su crecimiento, pero evitar estrés lumínico (en el día 21 se ajusta la iluminación a 55cm del canopy), se agrega un humidificador HUMIPRO para mantener estable entre 55 y 65 % la Humedad, intractor de 100mm, extractor de 100mm, filtro de carbon, ventilador "oscilofan", ventilador "clip fan", 2 termohigrometros y se agrega el día 20 una malla SCROG de kanovi Acompáñenme para ver los resultados de este hermoso desafío, un saludo cultivadores 🔥🔥🔥

This week, there started to be pistals forming on the largest plant, the medium plant and just starting on the smallest. I have added some nutrients from big buds, but I need some bloom first. ordered advanced nutrients. I trimmed off the lower leaves below the canopy to push them more into blooming. This is auto, so I have not adjusted lighting still 18/6

Start of week 11, ladies are doing good and continuing to fatten up. Looks like #2 is pretty close to being done. Going to let her run through Wednesday, then check on her trichomes. If we got more amber I’ll begin the 3 day flush and chop her down on Saturday. If I feel she can go longer I won’t start the flush until Saturday/Sunday. Plants #1 and #3 still got another two weeks or so before they’re ready to chop. Day 72, I moved plants #1 and #2 to the front of the tent for easier access. They’ll be the first two done out of the 3 included in this journal. Also cleaned up some of the leaves on them to open up everything to light during these last 1-2 weeks. Still unsure if I’ll begin to flush #2 on Wednesday or wait till the weekend.. Day 74, checked trichomes and we still aren’t quite where we wanna be before I start to flush. I’m in DWC my flush is only about 3 days tops. We will check again start of next week and hopefully she ready. Day 76, checked on #2 she still not quite ready. I will probably let her run through Thursday next week, begin flush then chop on Sunday the 6th.

Final week, will chop them a bit early in a few days

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Starting of the stretch getting way too tall lol

Hello Growmies, As we observe the growth in week 15, I'm filled with both anticipation and awe. She continues her impressive swell, and new white hairs have emerged, suggesting that the end of January might be too soon for harvest. The delicate balance of maturity is playing out before us, as the first few trichomes take on an amber coloration, signaling the onset of peak ripeness. There's a noticeable increase in her aromatic presence – she's getting louder, so to speak, filling the space with her potent scent. A beautiful fade is underway; the lower canopy leaves have turned a bright yellow and are gracefully falling away, a natural progression as she directs her energy to the buds. These, in turn, are taking on a striking purple hue, which is becoming more pronounced day by day. Each day brings a change, a new layer of complexity to her form. The way she's maturing, the hues she's revealing, and the scents she's exuding all contribute to the feeling that she's one of the most remarkable plants I've grown. Her feeding remains consistent at 4 liters of nutrient solution every four days, sustaining her through this critical phase. The light spectrums continue at full capacity, and she seems to savor every photon that falls upon her. With new growth still apparent, I'm considering adjusting the harvest timeline. Patience is key, as is careful observation of her trichomes and overall health. We're entering what may be the final stretch, but only time will tell. The yellowing of her leaves, the purpling of her buds, and the sweet, loud fragrance she's giving off are all signs of a successful grow coming to fruition. Stay tuned for next week's entry, where we'll delve deeper into her readiness for harvest and the splendid transformation that's taking place. Stay Lifted Salokin

WEEK 2 DAY 14 Since last week the babies have taken off! They didn’t have much light until yesterday when I added another 100w led and since then I’ve seen a huge difference ! They are looking super healthy the roots said fuck the pot and grew right through it I’m actually super interested in seeing how these grow up.. The 3 clones that were separate last week are now moved into one single pot to further develop the experiment. They are on the same feed as the mother with flora grow/micro/bloom and cal-mag . And In the last picture you can see my ghettos man way of cloning .. going to see which 2 out of 4 are better rooted and I will move those to a pot in about a week or so. If you guys have any questions or want to know where I got these exotic seeds message me!! Or comment below !