Hey guys! 👏 Hope everyone is doing well! This week was interesting. I made a small flush because I wanted to know how much salt my plants had in the soil (considering I've fed my plants with a lot of nutrients the past few weeks) aaaaand the results were quite interesting! They obviously had tons of salts! Here are the results of the runoff water: Falco 5.94 pH 1139 ppm (2278 us/m) Max 5.85 pH 2352 ppm (4704 us/m) Roko 5.64 pH 2453 ppm (4906 us/m) Boomer 5.72 pH 1765 ppm (3530 us/m) Surprisingly enough the plants that had the "worst" runoff values were the plants who had the most foliage and their leaves were the greenest. The pH "loss" of all the plants was also within the expected margin so that's good! 👌 The buds look beautiful and I also noticed some of the plant leaves are starting to turn purple 😍 the smell is also getting stronger and it's quite citric, I love it! Anyway, that's all for now. Thanks for reading! 🙌

Start of week 3. Weather conditions got better, less clouds and more sun seem to be good for the plants. 06/13/2020 -day 15- duck #1 still seems to have some problem. She grows but has a lighter greenish-yellowish color compared with her sister. Also the leafs have strange curls. I got a hint to remove the bio tabs and only give water. Maybe that will do the trick 06/16/2020 -day 18- finally a visible progress in growth. Duck #1 got back to a more natural green (thanks to a hint from Fusion 👍). Because of heavy rain, I got enough water for the two ladies 06/19/2020 -day 21- end of week 3, the ladies grew very good and got nice new leafs. So far, it really looks very decent an not like cannabis at all. Although they seem to be small in comparison with other diaries. This weeks weather was often cloudy with a lack of sun. Temperatures were between 25 C at day and 18 C at night. All in all I am happy that they seem to be healthy and growing. Looking forward to the progress in week 4. Keep in Touch ! Cheers HighZenBerg

She’s slowly but surly putting on weight . Trying my best not to harvest too early.

Temps have been good and RH has been steady between 60-65%. I have good airflow all around and don’t worry too much about it the higher RH at this point in the grow. Transplanted the ladies from their starter pots to their 2 gallon pots. They did not seem to have any transplant shock. A few more weeks and we will go to their 4 gallon final pots.

I messed up and didnt get many pics as me and my buddy were very very busy trimming from 10am-10pm got the 1 blue cheese plant finished!!! In 12 hours we did 1 plant thats fricken insane considering me and him did 8 of his outdoor plants in less time back in fall,more bud than i have ever seen on a plant under 3ft shit it had more bud on it than the 8 footer i grew last fall lol just so impressed with this plant i will definitely be growing many more blue cheese using FOOP nutes in the future thats for sure ,i am not touching any of this nugget until its dried and cured...gonna be hard to resist lol but it will be worth it for sure 😉

Super busy with a move and a big garden reno. Sorry, this is the only update for this week

All healthy lil ladies..

IRRIGATION 6.5 LT PER POTS GROW 1 LT / 4 ML MICRO 1 LT / 2 ML BLOOM 1 LT / 4 ML 1 LT/ 3ML BIG BUD 1 LT/2ML B-52 1 LT/ 2ML BUD CANDY 1 LT/ 2ML NIRVANA 1 LT/ 2ML SENSIZYM 1 LT /1 ML CAL & MAG PPM 1040 (TNB CO2 ENHANCER) PH 6.1

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 78: I checked the trichomes today and I'm pretty sure I saw cloudy ones. Hard to say even with the microscope, but I decided to wait at least until tomorrow before flushing. So I fed them today with the same solution as the previous week. Day 79: I saw more than one cloudy trichomes this morning so the final flush was given. This should, SHOULD, now be a smooth ride until the end. The buds are stacked, packed with trichomes and... damn that smell! Day 80: Nothing to read Day 81: Nothing to read Day 82: First watering since the flush with tap water, nothing else to read. Day 83: Nothing to read Day 84: I don't think they'll all be ready at the same time. I think they'll go in clockwise order starting with the bottom left one.

Papaya sherbet is doing well. Been looking Hard for any signs of herm She looks like she’s doing OK so far fourth week of flower and the buds are stacking nice💪🔥

This plant is really getting big. I had to do a lot of topping and defoliation this week. I wasn’t planning on any more topping but she is getting too tall and I won’t be able to pull poly over her when she is in flower if she keep stretching upwards. Humboldt doesn’t lie when they tell you the size their plants can reach!! 🌱🌱 😎🍿🍻🌱

in a quick way, it was possible to update my latest growths. now that everything is up to date, I'm going to talk about honeycream. 🐝🍯 look how that little one is getting ... Did some defoliation in week before. second week of bloom is completely frosty ❄️ I'll try to register everything but I don't promise anything ehehehee . see you soon 😃😴

I have been super excited with everything up to this point . I have made all my preparations that I think need to be made and did some trial runs on my environment. I starting preparing around the 15th of January and my set up was complete around the 19th of February. I spaced my purchases out based on budget constraints. I wanted a cheap and efficient grow area. Some thing I paid more for because through all the research I did I felt quality was more important. If you are as excited as I was, you get pretty anxious when awaiting order status when you first order your seeds. You should remember you are ordering from an online seed bank not amazon. Most work Monday - Friday and are in a complete different time zone. I ordered my seeds from seed supreme Seedbank. I placed my order on 31 January and mailed money as directed. This was the scariest part for me especially when I didn’t hear anything back. I finally received a email on the 12th if February saying my invoice was paid and then subsequently mailed out on the 15th and I received on the 20th. One thing I would like to note that recommend to anyone mail cash is to make sure you spend the extra cash and track your payment. I’m currently germinating all five GSC I received I placed them in water for the first 24 hours and then a paper towel for approximately 40 Hours. I’ll add links to most of the items I purchased below. Some items I purchased through offer up and let it go app to save money. VIVOSUN 48" X48" X 80" Hydroponic... https://www.amazon.com/dp/B07GNBCVPZ?ref=ppx_pop_mob_ap_share LED Grow Light 1000W Full... https://www.amazon.com/dp/B074MBP32M?ref=ppx_pop_mob_ap_share AcuRite 00613 Humidity Monitor... https://www.amazon.com/dp/B0013BKDO8?ref=ppx_pop_mob_ap_share Hydrofarm Grow!T Premium Coco... https://www.amazon.com/dp/B01DA4PZI4?ref=ppx_pop_mob_ap_share iPower LGTESTSOIL 3 in 1 Soil... https://www.amazon.com/dp/B075LRY5M7?ref=ppx_pop_mob_ap_share Unco Industries (WWSB15LB) Wiggle... https://www.amazon.com/dp/B00062KQ42?ref=ppx_pop_mob_ap_share General Hydroponics pH Control Kit https://www.amazon.com/dp/B000BNKWZY?ref=ppx_pop_mob_ap_share VIVOSUN Air Filtration Kit: 4... https://www.amazon.com/dp/B06XFRNPR8?ref=ppx_pop_mob_ap_share VIVOSUN pH & TDS Meter Combo, 0.05ph High Accuracy Pen Type pH Meter & +/- 2% Readout Accuracy 3-in-1 TDS EC Temperature Meter https://www.amazon.com/dp/B06XKMH86J/ref=cm_sw_r_cp_api_i_2xyCCbK4MKNN4 Botanicare CAL-MAG Plus Plant Supplement 2-0-0 Formula, 1 Quart https://www.amazon.com/dp/B004JKBMRW/ref=cm_sw_r_cp_api_i_LyyCCbY8SMFTK Century 24 Hour Plug-in Mechanical Timer Grounded https://www.amazon.com/dp/B00MVFF59S/ref=cm_sw_r_cp_api_i_ozyCCbHG8Q2PB Day 1 - 2/23/19: I planted my seeds today about a fingernail length down with tap root pointed and gave a light watering with plain tap water at a PH of 5.8. Day 2 - 2/24/19: Nothing new to report no activity on the surface, misted watering at 5.8 PH. Day 3 - 2/25/19: Plant number 1 which had the longest tap root broke the surface I started a time lapse on it to cover the first moments of birth 😭 daddy is already so proud lmao. I have another light watering today with my humidity domes on, the intent is to have the plants roots work to get the required water and hopefully promote root growth. I also added 1ml of Rapid start and diluted into 1 gal. Day 4 - 2/26/19: Checked the status this morning prior to work and two more have reached the surface only two more remaining which I suspect will reach by the time I make it home from work. Misted this morning pot was looking pretty dry with the exception of the humidity domes, which I removed on all the sprouted seedling. I intend on shifting to a 20 on 4 off cycle and starting my other fans. Day 5 - 2/27/19: All five plants have broke the surface. I did a normal watering this am still utilizing the dome to avoid watering on top of the plants. I lowered the light again some of the early sprouters we stretching a little more than I wanted and appeared leggy to me. Day 6 - 2/28/19: Nothing really to report today. Excited for the name reveal tomorrow. I did a light watering. I played around with my ppm meter and ec I definitely plan on using in conjunction with my first feeding this weekend. Day 7 - 3/1/19: Was watering this morning normal levels. I also mixed a new feed to be ready for them tomorrow. While I was watering # 5 fell over I accidentally tipped it with the spout. I grabbed some left over soul to help prop it up hopefully she is ok.

4weeks into flowering, halfway she is starting to smell mostly sweet, never got a chance to water her much last week, had to move the plans was pulling to much attention there.

Water from day 50 abit early but everytime I grow fastbuds they are fast lol usually 65 days for me but that's with my advanced nutrients I've grown other strains like blue cheese exdous cheese chronic big buddha cheese pineapple express power plant with little to no food for the whole like of the plant and still got nice colas not as meny but very good smell and taste end results if I can get decent yeild with just these nutrients il be very pleased fast buds kush is doing really well possible 2.5oz of organic weed not bad conditions are budget effort made was low and still shows great growth

Lets see

It’s spreading out really nicely and quite slowly as hoped. Defoliation and bending continues until flowering hopefully I should manage with space 😱😱😱😂

I use mycorrhizae.