Arrivato fresco fresco dalla Spagna si pianta subito. 12/01 12 h in acqua 13/01 l'ho messo in un tovagliolo di carta umido ed è è uscita la prima parte di radice ed è stato trapiantato in un vasetto. 14/01 e uscito dalla terra sfogliando le prime 2 foglioline tonde. Questa è una super partenza 72 h e già cerca la luce.

Finishing the last plant, the other 2 got harvested at week 10 and 11

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

-Grow is going great. Swelling up -I've been dropping the temps and humidity down during lights off. The temps don't drop below 66 degrees and I try to bring the humidity level down to 30-35% several times throughout the dark period. -Starting my flush. On my hydro plants, I will be using florakleen and hydroguard in water with a ph level of 6.0-6.2. -Flushing the plants in coco with ph balanced water and blackstrap molasses for the remainder of the grow. A nice experiment to see what difference it makes.

- Big Bud + Overdrive

Missed a week as been busy but here they are coming into week 10 time to start hitting them with some overdrive for a few weeks before flushing with ph,d water

They bounced back really nice from the topping last week, did some more defoliation and let them heal from the HST. I'm considering topping them again but I don't know if it's time for flowering?!

Muy Buenas señores! seguimos a la espera del equipo Led, Entramos en semana 2 de Flora con muy buen color esperemos conseguir mantener esa línea...las Royal Gorila están verdaderamente monstruosas, esta tanda pondremos menos fertilizantes por usar Mykos. Empieza lo bueno en breves veremos que tal va la resina con este calor. Salut e força al canut Farmers!

Transitioning to LED this week, going to ease into it. Topped at 21 days. Growing Lost Pearl and Critical Kush (which I plan to push into flower early.

la semana empiezan mostrando un estado muy bonito y gozan de humidificador nuevo. Primeras comprobaciones de temperatura y humedad con el humidificador al mínimo, como puede verse en la foto del dia antes, dia 15, apenas no ha cosumido nada de agua por lo que aumentaremos el caudal y tiempo en el temporizador analógico. Así aumentaremos también un poco más la humedad sin tantos altos y bajos Pasados unos 5 dias volví a podar dejando únicamente un nudo por planta y un doblado de ramitas. A los 21 dias volví a podar del primer nudo de cada rama, hojas y yemas.

Поливаю водой

week one ( day 1-7) went great , i germinated both seed in paper towels and transferred them to big pots . 1 (30 liter pot) !(25 liter pot). after that i got them outside in the sun as much as i could (it was pretty grey and bad weather) while keeping the soil damp with a spraybottle and keeping a sliced bottle over the top to increase humidity. just had to wait for my tent and lights to arive the first week.

Wow she grew loads this week!!!!!!! She actually over tripled her height! From 21cm to 69! Incredible. Look at all her lower branches too! The massive internode length means they're almost as tall as the main cola!!!!!!!!

So langsam legt sie mit der Trichombildung los und der Geruch verändert sich deutlich 💚

Week 13: Week 5 Flower—A Garden in Full Bloom 🌸✨ Hey, Grow Fam! 💚 We’re deep into Week 13, and this room is nothing short of mesmerizing. Each plant is unique, showcasing its trichome-coated beauty, with white hairs dancing in their distinct patterns. It’s the culmination of patience, hard work, and overcoming challenges. The two-layered canopy—thanks to some ambitious young ones—is a testament to adaptability and dedication. 🌱🌟 Feeding Update: Aptus Holland Breakout Powder This week, we introduced Aptus Holland Breakout Powder into the feeding mix at 0.5g/L, alongside the nutrients already in use. This powerhouse additive is designed to promote robust flower development, enhancing both density and resin production. Breakout Powder brings that extra “oomph” to the table, helping the girls truly break out into their full flowering potential. 🌺💪 Combined with our existing recipe, the results are already starting to show. Buds are swelling, trichomes are shimmering, and the room smells heavenly. If you’ve ever wondered what a well-rounded feeding schedule can do, this week’s results are the answer! TrolMaster Magic: Monitoring & Stability I’ve included a few TrolMaster app prints to share my love for this system. It’s not just about controlling the environment; it’s about mastering it. The app provides real-time data, historical records, and an unmatched level of convenience. Whether I’m sipping coffee or working in the garden, the system keeps me in sync with my grow. This week, I’m particularly thrilled about how stable the pH levels have become since transitioning to full mineral-based nutrients. Unlike the organic-mineral combo, which required frequent adjustments, the current setup is practically self-sufficient. This stability means happier roots, more consistent nutrient uptake, and ultimately, better plants. The data speaks for itself—check out the comparison print! Comfort & Style: My Aptus Holland Pants Oh, and can we talk about how comfortable my Aptus Holland pants are? These have become my go-to garden gear. Stylish, functional, and perfect for spending long hours among the plants. Let’s just say, my plants aren’t the only ones dressed to impress! 😂👖 Shoutouts & Gratitude As always, a huge thank you to the amazing people and brands that make this journey possible: • TrolMaster for the tech that keeps me connected to my grow. • Aptus Holland for the nutrients that take my plants to the next level. • Pro Mix for the growing medium that supports this foundation. • Cannakan for ensuring my seeds get the perfect start. • And of course, the seed banks for these incredible genetics. A special shoutout to the community—followers, friends, supporters, and even the haters. You all bring energy to this space, and it’s all apprecialoved! Let’s keep growing stronger together. 💚 #TrolMaster #AptusHolland #GrowLife #CannabisCommunity #FlowerWeek4 #NeverGiveUp #GrowWithLove #IndoorHorticulture #Defoliation #PPFD #ControlledEnvironment #SCROGNet #DogDoctorOfficial Discount Codes so you can save big on your next check out 💚💚💚 Kannabia - DOGDOCTOR 30% off SeedsmanSeeds - DOGDOCTOR 10% off CannaKan- DOGDOCTOR 15% off terpyz.eu - DOCTOR 15% off The Neutralizer - PORKIT5-DOG 15% off Fast Buds - DOGDOCT 15% off As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and so joyful with you all in my life 🙏
 With true love comes happiness 💚🙏 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so 💚 Friendly reminder all you see here is pure research and for educational purposes only Growers Love to you all 💚💚💚

The first picture day 44! Well, we've finally got the purple colours coming in very strongly! Even the fan leaves are turning purple. The progression of the pigment looks like a pathogen has taken over but it's just the cool genetics of this plant. Smell is increasing, they have a very sweet odor so far when crushing the flowers. Still nowhere near harvest but the buds and calyxes are starting to stack. Very exciting that I got this one to grow!

Harvest Day

Day 39/40 of the adventure, just trimming and letting them cook...

Another good week. They got their first real water twice this week. They are enjoying the high humidity and temps. Right on track so far and hoping they keep it up!