Things are progressing nicely. Will put it in flower tent in next week or 2. Second plant of perpetual grow ended up being a dud. A blackberry kush seed has recently been sowed. Photos/video taken 56 days after breaking soil.

Woche 4 – Übergang zur Vorblüte 🌸 In dieser Woche habe ich überwiegend mit Wasser gegossen und einmal zusätzlich Dünger nach dem Blüteschema von Hesi gegeben, da sich im Laufe der Woche erste Anzeichen der Vorblüte gezeigt haben. Insgesamt haben sich die Pflanzen sehr gesund entwickelt und sind weiterhin gut gewachsen. ___________________________________________ Week 4 – Transition to Pre-Flower 🌸 During this week, I mostly watered with plain water and fed once using the bloom feeding schedule from Hesi, as the first signs of pre-flower started to appear. Overall, the plants remained very healthy and continued to grow well.

If you’ve followed along you’ll know how well this plant grew. It handled a lot of stress from supercropping, LST, and defoliation. The plant wanted to produce and wasn’t letting me get in the way of that. 7 days dry in my new dry and curing room. First 4 days 70*f and 55% humidity increased to 60 by the final day. I ended up with just over 6 ounces dry weight, well trimmed, and properly weighed. My seedsman Gelat.OG grown at the same time was an 8oz plant and the only one that beat the lemon pie on dry weight. The smell and colors of the lemon pie had me going crazy from early flower til the end. It was an absolute pleasure to grow. This is the second Lemon pie I’ve finished, a friend was running out of room so I finished his plant. He also got close to 6ozs but the smoke was much different and the colors were as well. You will run into very different phenos with fastbuds strains and it makes it difficult to repeat grows, just don’t be upset if my smoke report above isn’t consistent on every plant if you decide to grow it. Both were very enjoyable though… FastBuds has a place in my heart forever and I’d recommend them to anyone looking to grow cannabis at any skill level. They’re just good people over there. Personally I will be taking a new journey with Mephisto next to try and expand my knowledge of other growers, after hearing so many great things it’s time I give them a try. Hope you follow me along on that grow as well.

Que pasa familia, vamos con la sexta semana de floración de estas Papaya Zoap F1 de Sweetseeds. Vamos al lío, me quede con 3 por espacio, siempre pongo alguna semilla de más por si no abriese alguna por no perder ese hueco del indoor. También se trasplantaron a su maceta definitiva, en este caso de 7 litros. Y también superaron el shock por el trasplante se recuperaron 100%. El ph se controla en 6.2 , la temperatura la tenemos entre 20/22 grados y la humedad ronda el 50%. El fotoperiodo esta a 12/12 para floración. Las jodidas estiraron bastante, y es algo que no me gusta de todas las F1, que cultive hasta ahora. El olor que desprende es muy peculiar es muy dulce y empalagoso, a mi parecer, no pinta nada mal. Hasta aquí todo, Buenos humos 💨💨💨

Buenos días familia, de vuelta actualizamos con la última semana en estado vegetativo, ya se cambió el fotoperiodo a 12/12 Y es que esta skunk están increíbles, tengo mucha curiosidad por ver que sale. Ph controlado en 6.5 , el alimento lo aceptaron muy bien. Temperatura algo baja por la noche, pero sin problema alguno, mínimas 17. Se comportan genial en indoor y no espigan nada de nada, bastante recomendable.

Day 15 of flower! Kinda goofed this week i gave the babies week two nutes after the last post wich was like day 10 and i noticed a little bit of nitrogen over dose. I think the Powersi is also helping nutrients absorption! So i actually did this week a little different then i planned just cut the bio bloom in half because i didnt realize the powersi npk ratio inceases p and k Havent seen anymore aphids might do another couple sprays to be safe. Grow safe allows full spray through flower

The gutter hoe grow show powered by the viparspectra XS2000 is still in action. The plants are showing that they aren't completely happy with the limited root space they have but they are growing some buds. Crescendo is definitely the most trich coated of the bunch. The big detroit energy is producing some big fat buds. Lowered the temp to 72. Other than that the juice will keep pumping and the lights will keep beaming.

Hello Diary, The Milky Way F1 is ready to harvest soon, although the Titan F1 ripened first and is already ready, the Milky Way F1 and Apollo F1 will have to wait another week in my estimation. The Milky Way F1, as seen in the photos, is truly a sight for the eyes. The flowers started to get more and more brown pistils, but looking under the microscope, the trichomes are not all milky yet. So I'll leave her for another day. Otherwise, the flowers are full of trichomes, from which a strong fragrance spreads. Here and there a yellowish leaf appears, which I immediately remove. Watering is a little more frequent due to the high temperatures, but I stopped adding any nutrients. Here's a quick recap of the week. 26/06/2023 - Day 58. Watering. Only two days later, the plants drank all the water, so I watered them with two liters for each individual plant. 29/06/2023 - Day 61. Watering. I repeated the procedure as I did three days earlier. 01/07/2023. - Day 63. Photographing plants. That's all for this week, see you soon.

Really just a video from this week but I think I was a week or 2 behind anyways lol just so y’all can see some real color of my girls. Still haven’t named em but I’ll be doing that in the next week I’m switching em to 12 gradually within the next week to experiment with some stretching during the transition compared to a straight 12 switch

Diese Woche habe ich sie noch einmal ordentlich entlaubt. Jetzt ist sie ready für die Blüte. Die letzten zwei Tage durfte sie in Ruhe chillen und sich erholen. Ab heute bekommt sie 75 % Lampenleistung. Ich hoffe auf richtig große, schwere, fett stinkende Buds.😅🤣😎

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

I think I'm on track. About 4.5-5 weeks left for these to fatten up. One of the clan (Cappadonna) started herming so I got rid of him real quick.

So I had to trim these ladies they desperately needed it These ladies are getting nice and smelly fruity smell filling the tent there are plenty of crystals coming and the buds are bulking out filling up the stick I am looking forward to these 🌱💚💨💯🙏

OMG, so much has happened since my last post, this is way overdue. My soil went totally hydrophobic, my PH pen was outta calibration so my soil ph was way of, combined with another gnat infestation. Things are much better know. the gnats are gone, transplanted into larger pots with newly bought soil, changed lighting to 18/6, vegged for a couple more weeks . One plant showed full male pollen sacs and one had suspicious looking male pre-flowers so I culled both. The last 2 showed what I think look like female pre-flowers. I just flipped lights to 12/12 on 4/4 so hopefully I have 2 females, started weaving thru scrog net, canopy looks fairly even, plants due look super healthy aside from couple tips have a bit of yellow But thats from early veg when I had the lights a tad too close. Wish me luck ;).

Been another week of crazy stretch for the sugar cane and apple sundae. Been supercropping several branches because they have maxed out my light height at over 4 ft tall. The slurricanes are looking much more manageable and it looks like the stretch is about over on all of them. I switched them over to maxibloom and added a little liquid koolbloom and Beastie blooms to start feeding those flowers. The room is definitely filled up now. just have to keep the juice pumping and see what they do.

Día 40 me ha dado carencias de calcio magnesio y posforo y potasio, me he dado cuenta un poco tarde... Las hojas estaban caídas y se me estaban poniendo amarillas con manchas marrones...les puse calmag y empezaron a buscar la luz ... También me di cuenta, que las Lili que no veía se me ponían las hojas moradas, creía que era exceso de nitrógeno, pero no era posforo y potasio... Así, que lo que no quería hacer, lo he hecho he metido a tope de nutrientes y más... Espero no liarla más...

Another good week beginning on Sunday they were switched to 12 12 and given a feed and back in today for a flush