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Defoliated 3 of the 4 2x Royal Critical and 1x F1 Orion. The zombie has started to grow will be a couple of weeks behind the rest. But its ALIVE its ALIVE šŸ˜‚. They are soaking up the water and will add nutrients from next week 6
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Pre flower formed, starting to smell a bit more than the last week, keeping grooowing šŸ’š
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Hi Growmies, Last week for my ladies, going to harvest any day. Not giving any nutrients .Conditions are perfect and 2 weeks no rain are predicted with temps around 18°. Thanks for swinging by .
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@Kardo
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Hat einen leichten Training bekommen ich bin gespannt wie du Buds aussehen werden 🤩
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7/4 SUN was poking out a little bit. Plants loved that little feeding and seen to he noticeable larger this morning. I'm considering starting the nutes. At least the organic ones as well as upping the water intake I've been scared to water with all this rain and my experience last time. I plan on applying BT this afternoon and doing some work on the bottoms of the plants. I also saw a chipmunk in my cage so I have some spots in my fence that need to be patched. I know I can't compare this year to orher years as these are seeds and all the other years were clones. I CAN say for certain that my plants are healthiest they've been out of any of my journals. Previous plants were much larger but I was fighting numerous fungal, pathogens and pests at the sane time. Ear wigs would've lollipopped most buddies by now but I've seen no sign of them. I'm going to apply more poison where they were and add some rat traps inside the cage for the chipmunks. Once I fix the cage I think I'll be good. UPDATE: Went back over and fixed the holes on my cage from high winds. I found an old wire trap or cage and just cut sections and ziptied them to the existing wire and to the structure thus covering any open holes. I'll need to put lathes aroubdvtge outside because if a rodent REALLY wanted in they could get in. My belt had been wearing thin but I use shit until it breaks (yesterday the heal came off my shoe lol) so I was working hard sweating my nuts off and my belt gives out totally. At least ive got the major stuff done. I took a few videos too. I'm imagining it was this little bit of sun that gave these plants that boost of growth but I'm wondering if the added nutes had anything to do with it. Bags weren't light and I could feel moisture in the soil so I didn't water. I'll check again tomorrow morning. Since I didn't see many plants or even leaves for that matter with damage I decided to hold off ob the BT and the plant doctor. Only time will tell if that was the right decision. 7/5 Plants look fantastic. I see a few more holes in leaves sporadically amd I'm hoping it's pillars. I've seen lots of lightning bugs on my camera though and grasshoppers are abundant. Not one growth shoot has been chewed (knock on wood) which is what the earwigs had always done. I watched a video from last year and by nowcthey had lollipopped all lower leaves. It may not be the best weather growing season wise but despite the rh consistently in the 90's I still don't have pm or fungal pathogens. Need to get the BT out. Didn't water as we've had all that rain. I'll water either tonight or tomorrow morning. Sunny high in the 90s low is 66. Tomorrow looks good too. UPDATE WENT BACK OVER AROUND FIVE. IT WAS 88 DEGREES SO I WANTED TO CHECK THE GROW BAGS. THE POTS ARE FINE. IT WAS HUMID AS HELL. ONE PLANT WAS SLIGHTLY DROOPY BUT VERY LIGHT AND DRY. I FOUND TWO OTHERS THAT SEEMED LIGHTER THAN THE REST BUT HADNT DROOPED. I MIXED TWO GALLONS OF WATER WITH 1TSP KANGOROOTS AMD PHED IT CLOSE TO 6. I FED THE 3 PLANTS THAT WERE SUPER DRY LIKE A HALF GALLON AND SPRED THE REST OVER THE OTHER EIGHT PLANTS. THEY WERENT DROOPING BUT THEY WERE VERY DRY. I PLAN ON A FULL WATER TOMORROW. I WOULDVE DONE IT TODAY IF THE TEMPS WERENT SO HIGH. ITS RAINED A MONTH STRAIGHT SO I HAVENT HAD TO WATER. I MADE A COUPLE VIDEOS BUT I'LL HAVE TO UPLOAD TOMORROW. 7/6 Another super hot day. I hefted the pots and they still had some weight but I could tell they were dry. This rain has messed up my watering schedule. Well it made it so I didn't NEED to water. These are big plants now. I need to get a schedule to stick too. They're going to probably need a gallon a piece at least. I'm still nervous watering. Right now I'm just reading the plants. I added .5tsp kelp me/you to 1 gallon of water to help with heat stress. I fed an additional two gallons to the garden this morning including the two container plants in the back. They were dry on top too but I know they have water deeper down. Next watering I'll be more consistent and try to give them there 10%. It's great having the bags elevated. I can finally see when I start getting run off. I could even measure the ph of it instead of relying on that meter. Ill check the ph when I go back over. Still a few šŸ› holes but they are few and far between. I really don't want to spray for such a small problem but if I cant find them at night that's what I'll do. UPDATE: Another 90 degree day. I went back over and gave the garden a gallon of water as they were dry and it didn't rain. Tried to use a soil ph meter to check ph. First couple were 6.4, 6.5 then I got 7.3 and I accidently dropped it. Then I got somethings that were high eights and even one 9! Obviously the Meter shit the bed. I'll lower the ph of the water slightly when I fully water tomorrow and I'm going to measure the runoff. 7/8 I must've messed up the journal again as the dates are off. GAVE PLANTS A FULL WATERING. Each girl got a gallon. I couldn't upload my videos this morning as I had to break up a fight. 3 on 1. Didn't give me a chance to put the videos up. I'll take some stills then I can upload them. I took stills and they all uploaded but didn't fucking save. I'm nit going to keep trying to upload if it's not going to. Noticed a few more holes in leaves and one skeletinized leaf so I need to either spray the bt or something more versatile. I'm putting more poison around the cage and de between the bags. I'll go check things out tonight. Sick of writing a book and uploading to just hace them disappear 7/9 Did a quick video today. Noticed the bags were fairly light despite the plants being soaked amd the pallets wet. I was pressed for time. I gave two gallons to the entire garden. I hope that will hold them over until tomorrow. I'll need to up how much water I give them. Going with a gallon and a half next time. I see more pillar spots and a moth took off when I shook the plants this morning. So I'm gonna have too apply the BT. I figure if I go over before dark tonight I might be able to see aju nocturnal insects around. Luckily my dad feeds the birds and they are always there. I think they help with pests. UPDATE IT WAS A LITTLE COOLER TODAY BUT MICH MORE HUMID. I WENT AROUND SIX TO MAKE SURE THE GIRLS WERE OK WITH WATER AND TO GIVE THEM A TREATMENT OF BT. I WENT THROUGH THE GARDEN ANDCGOUND THREE LEQVES TO DEFOLIATE AND LITERALLY A COUPLE LEAVES ON TWO PLANTS WITH PILLAR HILESM WHEN I ARRIVED TWO BIRDS FLEW OUT. A HORNET CAME IN WHILE I WAS THERE. THIS DO LESS APPROACH SEEMS TO BE REALLY WORKING. I DODNT SEE NEARLT ENOUGH DAMAGE TO WARRANT SPRAY8NG MY EXTREMELY HEALTHY GIROS WITH ANYTHING. ILL KEEP AN EYE ON THEM AND CLEAN THEM UP A BIT. I DO NEED TO INSTALL THE VERY8CAL TRELLIS FOR SUPPORT. THEYRE PRETTY HEALTHY THOUGH. ONE PLANT IS ABOUT AS WIDE AS IT IS TALL. STILL......PLANTS ARE EXPLODING AND ITS GETTING TIGHT IN THERE. I NEED TO GET THE TRELLIS UP THOUGH. 7/10 I went over and was planning to water. Plants were wet and it's raining. Top of the medium was moist but the bags were light. ONE bag was super light but seeing that we are having showers all day and an additional half inch of rain coming tonight so I didn't water them. Especially since they looked great. Decided to to spray BT yet as the damage is so small and i think the birds have been taking care of the pillars for me. Now I'm wondering if I should've gave that ONE plant a little bit of water but it will be find. Did a video. I'm being careful not to over water. Last year this is when all my buddies were devoured by earwigs. And no senescence like the years before. I think it was hust those earwigs. I haven't lost shot for leaves. Even the stalks are bright green and look amazing.
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Buds are really setting up nicely. French Macaron smells right on brand... and the Chem Brulee... my god! It smells like a Tropical Rocket Fuel. Amazong. The Pineapple Meatball has a really unique odor, but it is still fairly faint. Hoping that the neem/nute burn didn't somehow affect its resin/terp/bud production. It certainly hasn't seemed to affect the French Macaron and the Chem Brulee! This week, I sprayed 3 times with Dr Zymes, and it completely knocked out the small number of fungus gnats I was dealing with. Also released 4,500 ladybugs, and it's been fun watching them spread and work. Will probably stick with the once-per-week Zymes treatment for the next few weeks.
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@Mo_Powers
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the weather in germany is still totally changeable. temperatures from 10 degrees at night to 30 degrees during the day. and lots of rain. she's still alive, that's the most important thing.
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@Ninjabuds
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Ive got two Skunk Apple Runz plants(tossed 1 when I repotted the plants) with exotic-looking leaves and some great genetics. These are seeds I made and both the parents were top notch. They're both about five inches tall and growing strong they both seem to have the growth strength from the ricky bobby Unfortunately, one of them might not make it because of some damage or mutation im not sure, it just seems to have stop growing upward correctly but the other one's looking really healthy. Happy late turkey day grow diaries world!!!! It's now day 24 from seed for all the seedlings I have going. The past few days the plants have really started to take off. There roots have really started to dig in. Every other water I am still giving 2ml per gal of fox farm grow big. I keep having to tell myself not to add any more nutes other than that. I don't want to fry my plants but I also want they to start jumping. I'm quite excited for the coming week, I think the next 7 days or so is when the plant really starts to transform and look more like a pot plant than just some random little green thing. A few days ago I decided to move the plants from under my 55w cheep amazon light to under my spider farmer 100w newer version light with the good leds in it. The plants will hopefully be ready to up pot them here soon and get them in the large tent. Sometimes when growing multiple different strains it can be really hard to balance giving to much or to little light. You don't want the faster growing plants to get to stretched out waiting on the slower plants to catch up I spend Thanksgiving day morning transplanting the plants into 2gal pots. I am useing spme promix potting soil it's my 1st time tring this soil. It is actually doing really well the roots were all white and nice looking. I think next time I'm going to add some worm casting, kelp, granular mycos, and a little bit extra perlite. I just didn't have the money with christmas coming to splurge for all the extra stuff. I got rid of 2 plants 1 of the bubble og and 1 skunk apple runtz. There is only room for 10 pots in the 2x4 tent. I will peob only bring 8 of them to flower, always good to have back ups. Yesterday I also stuck the plants back under the 500w medicgrow mini sun 2 in my 2x4 tent. They did great under it the 2nd half the day I was happy they didn't get stressed. I had put them under that light a little early about 10 days ago or so. I am expecting in a week these plants will ge going crazy with growth
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@Siriuz
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Week 12 Day 79 Started flushing this week She's almost ready for harvest We plan on to next week Stay tuned for more Day 80 She's starting to smell more and more after we added so much water to drain all nutes Day 81 Looking good and can't wait to smoke some soon! Day 82 Good Day 83 She's good Day 84 check out the video sorry for the late updates I've been busy being a dad of a newborn baby boy :) Day 85 End of week 12 Everything looking neat
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Este sueƱo es posible gracias a Royal Queen Seeds, Marshydro, XpertNutrients y Trolmaster, sin ellos esto no se podria cumplir . šŸ’šŸ Dynamite Diesel: Royal Queen Seeds se ha asociado con Tyson 2.0 para ofrecer la genĆ©tica desarrollada por este campeón en forma de semillas. La Dynamite Diesel recibe su nombre en honor al apodo de Mike Tyson al inicio de su carrera, Kid Dynamite. Es un cruce entre dos variedades mĆ­ticas, la Sour Diesel y la Skywalker OG, que son conocidas por sus intensos sabores y sus efectos estimulantes,tiene un 22% de THC, lo que la sitĆŗa en la categorĆ­a de peso crucero. En interior, esta variedad crece a alturas de 100-150cm y produce hasta 550g/m² tras una fase de floración de unas 9 semanas. Al aire libre, puede alcanzar hasta 2m de altura y produce una cosecha mĆ”xima de 675g por planta. šŸš€ Consigue aqui tus semillas: https://www.royalqueenseeds.es/rqs-semillas-cannabis-tyson/664-dynamite-diesel.html šŸ’”TS-3000 + TS-1000: se usaran dos de las lĆ”mparas de la serie TS de Marshydro, para cubrir todas las necesidades de las plantas durante el ciclo de cultivo, uso las dos lĆ”mparas en floracion para llegar a toda la carpa de 1.50 x 1.50 x 1.80. https://marshydro.eu/products/mars-hydro-ts-3000-led-grow-light/ šŸ  : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit šŸŒ¬ļøšŸ’Ø Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ šŸ’» Trolmaster Tent-X TCS-1 como controlador de luz, optimiza tu cultivo con la Ćŗltima tecnologĆ­a del mercado, desde donde puedes controlar todos los parametros. https://www.trolmaster.com/Products/Details/TCS-1 šŸ£šŸ¦šŸŒ“ Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes lĆ­quidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ šŸ“† Semana 2: Gran primera semana, ella ha dado un gran estiron estos dĆ­as, si sigue asĆ­ serĆ” una gran cosecha. Esta semana se practica defoliacion, poda de ramas bajas y se le agrega Sticky Fingers . La carpa estĆ” ocupada al 100%, sigue una floracion explosiva gracias a @Marshydro y @Xpertnutrients y @Trolmaster con esta gran genĆ©tica šŸ’Ŗ. Potencia de la lĆ”mpara: 70%
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Hallo zusammen šŸ¤™. Habe sie heute geerntet. Lassse sie jetzt 3 Wochen trocknen und komme dann mit dem Erntebericht zurück. Bis in 3 Wochen šŸ¤™ https://seedsmafia.com/de/
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19. MƤrz Es riecht sehr wild sobald man das Zelt aufmacht:) Wir kommen voran. Meiner Ak geht es wieder gut. Ich Idiot hab vergessen seit der SƤmlingsphase einen der Ventilatoren immer weiter nach oben zu schieben. Die Dame hatte einfach Hitze oben x) hƤtte ich früher von selber drauf kommen kƶnnen šŸ˜… aber jetzt ist es halt so. Die Purple Punch sieht trotz Nanners wunderschƶn aus und reift unglaublich gut kann mich weiterhin kein Stück beklagen weitergemacht zu haben :) Des Weiteren habe ich ein kleines Upgrade vor: Hab mir von Ac Infinity die S16 Lampen gekauft. Ich warte noch bis der Postbote meine Zusatzstsngen für das Zelt liefert u d dann kann ich es installieren. Ich bin sehr gespannt ob das was bringt :D
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šŸ’œ Purple Haze by Zamnesia – Pheno B Week 8 | The Bush With Her Own Rhythm Welcome back to another chapter of the 8Ɨ8 Adventure! Last week I talked about how remarkably similar the two Purple Haze sisters had been looking. Well... That story has changed. šŸ˜„ This week, Purple Haze Pheno B is making it very clear that she has her own personality. While her sister has developed a different structure and expression, Pheno B has settled into something completely different: She's a bush. šŸŒæšŸ’œ Compact. Dense. Lush. Full of life. And absolutely comfortable being exactly what she is. This is one of the reasons I love growing multiple phenotypes from the same genetics. You can give two sisters exactly the same environment, exactly the same nutrition and exactly the same light... And eventually, genetics starts whispering: "Let me show you who I am." And this girl is definitely talking. 🌱 One Project. One Environment. Twelve Unique Stories. If you're new to the diary, welcome! This plant is part of my ongoing 8Ɨ8 Adventure, where twelve different cultivars are being grown together using the 12/12 From Seed technique. From the beginning, every plant has received the same opportunity: • The same lighting schedule • The same Plagron nutrition • The same AutoPot irrigation system • The same Future of Grow lighting • The same TrolMaster-controlled environment • The same temperatures • The same humidity No individual feeding. No special environmental treatment. No different light schedule. The genetics are the variable. And now, eight weeks into flowering, those genetic differences are becoming increasingly obvious. That's the beauty of this project. One environment. Twelve stories. And Purple Haze Pheno B is writing hers in a very different handwriting from her sister. 🌿 The Compact Purple Haze If I had to describe this phenotype in just a few words, I would say: Compact. Bushy. Lush. She's not trying to become the tallest plant in the room. Instead, she's building herself outward. Her branches are packed closely together, her foliage is abundant, and flowering sites are appearing throughout that dense structure. Looking at her now, I can see why she has developed into such a compact plant. There is simply a lot of plant packed into a relatively small space. And I love it. She has created her own little ecosystem. Everywhere I look, there are leaves. Behind those leaves, more branches. And behind those branches... flowers. 🌸 This is a phenotype that seems much more interested in building density than chasing height. And that's perfectly fine with me. 🌸 Week 8 – The Flowers Are Taking Shape The flowering sites are becoming much more established now. The delicate little clusters from the earlier weeks have developed into recognizable young flowers, with pistils continuing to emerge from the developing calyxes. There is still plenty of time for these flowers to swell and mature, but the direction is becoming very clear. She's flowering. She's stacking. And she's doing it everywhere. One thing that really stands out in the photographs this week is just how much healthy foliage surrounds the flowers. The plant remains intensely green and full of vitality. There is no shortage of leaves here. In fact... there may be a few too many. šŸ˜„ Which brings me to what's coming next. āœ‚ļø The Defoliation Is Coming Until now, I've deliberately allowed this girl to keep her healthy foliage. I wanted to see how she developed naturally before deciding how much intervention she actually needed. And I'm glad I waited. Because now I can see exactly what I'm dealing with. She's lush. Very lush. The canopy is becoming increasingly dense, and as flowering progresses, I want to make sure the developing flowers have enough space, airflow and light penetration. So I believe next week will probably be the right moment for some selective defoliation. Not a dramatic haircut. Not stripping the plant bare. Just a thoughtful cleanup. Removing selected leaves that are: • Blocking developing flowering sites • Creating unnecessary layers of foliage • Restricting airflow inside the bush • Sitting in places where they provide little additional benefit The goal isn't to change who she is. The goal is to help her express what she's already built. And honestly... I think she's going to shine after it. ✨ šŸƒ Keeping the Plant Healthy One thing I continue to appreciate about this phenotype is the overall quality of her foliage. The leaves are broad, healthy and strongly developed. The plant has maintained excellent colour and structure, and there are no major signs that she's struggling with the demands of flowering. This is particularly important at this stage. A plant can be large and impressive while still being stressed. That's not what I'm seeing here. Instead, she looks comfortable. She's growing at her own speed. She's building flowers at her own speed. And she's maintaining her own balance. That's exactly what I want to see. šŸ’§ Feeding for Week 8 As flowering progresses, the feeding program continues to support the plant through this important period of flower development. This week's Plagron feeding consists of: • Terra Bloom – 2.2 ml/L Supporting the nutritional demands of flowering and continued flower development. • Power Buds – 1 ml/L Supporting the transition and development of the flowering sites. • Green Sensation – 1 ml/L Providing additional support during the flowering phase as the flowers continue developing. • Sugar Royal – 1 ml/L Part of the flowering nutrition strategy supporting the plant as she progresses toward maturity. • pH Plus – 0.03 ml/L • Lemon Kick – 0.03 ml/L Used as required to bring the nutrient solution into the desired pH range. The current nutrient solution is sitting around EC 2.0 mS/cm, with a solution temperature of approximately 24°C. And, as always... every plant in this project receives the same recipe. The phenotype decides how she responds. šŸ’” Light From Above and Below The combination of the main Future of Grow lighting and the Lumiflora under-canopy lighting continues to be particularly interesting with this phenotype. Because Pheno B has such a dense, bushy structure, the additional light lower in the canopy becomes especially relevant. The upper canopy naturally receives the strongest illumination. But with a plant this compact, there are flowering sites developing throughout the interior and lower portions of the structure. The under-canopy lighting provides another layer of illumination in those areas. Again, I'm not expecting miracles. It's simply another tool in the ecosystem. And as I selectively remove some of the excess foliage next week, I'm particularly curious to see how the combination of improved airflow, better visibility of the flowering sites and additional lower-canopy light affects her development. šŸŒ”ļø A Stable Environment The room continues to provide a consistent environment for all twelve phenotypes. Current conditions for this update: • Flower Week: 8 • Height: 111 cm • Light schedule: 12/12 • Day temperature: 20°C • Night temperature: 25°C • Relative humidity: 60% • pH: approximately 6.0 • EC: approximately 2.0 mS/cm • Solution temperature: 24°C • Substrate temperature: 21°C • COā‚‚: 636 ppm • Watering: approximately 1.2 L per plant / 24h • Pot size: 15 L Note: the temperature values are recorded exactly as shown in this week's GrowDiaries data. Consistency continues to be one of the most important parts of this experiment. The environment stays consistent. The feeding stays consistent. The plants don't. And that's exactly what we're here to observe. šŸ“· This Week's Gallery This week's photographs really tell her story. The wider shots show a plant that has become incredibly dense and compact, with foliage filling almost every available space. The closer photographs reveal something even more interesting. Behind that wall of leaves are flowering sites developing throughout the plant. The pistils are fresh. The flowers are forming. And the resin production is already becoming visible across the developing structures and surrounding sugar leaves. She's not trying to be her sister. She's not trying to be the tallest. She's not trying to follow the same path. She's simply being Purple Haze Pheno B. And I think that's beautiful. šŸ”® Looking Ahead – Time to Let Her Shine Next week may be an important little turning point for this girl. After allowing her to develop naturally for so long, I think she's finally reaching the point where a selective defoliation will benefit the structure she's created. I'll be looking for the leaves that are doing more blocking than contributing. I'll open some of the interior. I'll improve airflow. I'll give those developing flowers a little more breathing room. But I won't take away her personality. That bushy structure is part of what makes this phenotype interesting. The goal isn't to turn her into her sister. The goal is to help this phenotype become the best version of herself. And I have a feeling that once we open her up a little... She's going to surprise us. šŸ’œ Different Doesn't Mean Less One of the biggest lessons from this 8Ɨ8 Adventure continues to be that there isn't just one definition of a great plant. Purple Haze Pheno A and Purple Haze Pheno B may share the same genetics. They may share the same room. They may share the same nutrients, lights, irrigation and environmental conditions. But they're telling different stories. And that's exactly what makes phenotype hunting so fascinating. Pheno B has chosen a different path. She's compact. She's dense. She's incredibly lush. She's building her own little world instead of reaching for the ceiling. And honestly? I'm loving her for it. šŸ’œ Every phenotype deserves the opportunity to shine in its own way. And next week, when we carefully open this beautiful bush and give those flowers a little more room to breathe... I think we're going to see just how much potential has been hiding underneath all that green. šŸ’š Thank You Thank you so much for following another chapter of the 8Ɨ8 Adventure. This project continues to remind me that growing isn't about forcing plants to become identical. It's about creating the right conditions... Then stepping back and watching genetics tell its story. A huge thank you to: šŸ’š GrowDiaries — for providing the platform where growers from around the world can document, share and learn together. 🌱 Zamnesia — for the Purple Haze genetics that are giving us these fascinating phenotype differences to explore. šŸ’œ Plagron — for supplying the nutrition supporting the plants throughout this journey. šŸ’” Future of Grow LED — for providing the lighting powering this project. 🌿 TrolMaster — for helping maintain the stable environment shared by all twelve phenotypes. ✨ Lumiflora — for bringing the under-canopy lighting into this experiment and giving us another variable to observe. And finally, thank you to everyone following these diaries, leaving comments, asking questions and enjoying this adventure with me. Last week, I thought these two Purple Haze sisters were following almost the same blueprint. This week, Pheno B looked at that blueprint and apparently said: "Nah... I'm doing my own thing." šŸ˜‚šŸ’œ And you know what? That's exactly what I wanted to see. Different phenotype. Different rhythm. Different story. Same beautiful genetics. Growers Love! šŸ’œšŸŒæ
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2/14: Decreased distance between the lights and canopy. Let the maximum swell commence! 2/16: Fed today..increased sugars/carbs. This was probably the last feeding with Beastie Bloomz..switching to Cha Ching at next feed and cutting back on the One.
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2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of dĆ©jĆ  vu. DĆ©jĆ  vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.
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Ultima semana de fertilización, a partir de hoy solo riegos con enzimas o con flush, creo que voy a dejarla unas dos semanas con este régimen. Me sorprendió esta auto, de gran aroma, se desarrollo muy bien y muy rÔpido, lastima que no sepa que variedad es.