|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 26 Summary - Ensign's log, stardate 17608.3, Wow what a week! Drying is always pretty simple and been checking daily. Buds are drying nicely and terps are smelling pretty dank!! Most of the week has been in the veg tent with tissue culture! Mixed another batch of establishment media and took proper explant cuttings. Also super-cropped the mothers to even out the canopy. Ahh yea! Getting hyped for trim day next week! Next week will also be the last week for this grow log, but am already planning out the next cycle. Anyways! Onwards to next week! [START OF WEEK 26] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk19] - WEEK 26 - Dry Wk1 ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 09 - Full Veg Gorilla Goat #1 - WEEK 05 - Full Veg Heady OG BX1 - WEEK 09 - Full Veg Garlic Budder OG#1 - WEEK 05 - Full Veg 10/13/25 → 10/19/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~0 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.5 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 00:00 - 00:00 | Wattage: ~000.0W [LV00] Distance From Canopy: ~00” DLI: ~0.00 mol/m²/d PPFD: ~0 μmol/m²/s VPD: ~1.17 kPa Soil %: [47.4, 34.1, 35.0, 55.7, 43.7, 43.6, 44.0, 43.3] ========================================================== Day 01 - Monday - 10/13/25 - Total Days: 183 ----------------------------------- Daily Averages ~[+01:00] - Temps: 78.8°F [26.0°C] - Humidity: 64.4% - VPD: 1.19 kPa - Soil %: [49, 34, 35, 57, 44, 44, 44, 42] ----------------------------------- - [22:05]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great! --- Heady OG BX1 #1 is drying along and no signs of mold. Also airflow and RH is stable and within the 60-70% range. --- Getting stoked! Trim day is 9 days away! - [22:09]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 40% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 40% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [22:18]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- All of the vegging mothers are looking great and the canopy is starting to even out. --- Also clones look healthy and no need to water yet. --- The stems, err, I mean, explants, don’t show any signs of contamination. --- Staying positive, glad the growth media is sterile and the overall procedure was successful. --- Anyways! Plan to create more establishment media and cut proper explants soon! - [22:21]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 10/14/25 - Total Days: 184 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.9°F [24.9°C] - Humidity: 64.8% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 43, 44, 43] ----------------------------------- - [21:44]: Night check on the 4x4 tent. --- Looking good in the dry tent! --- Heady OG is drying along and humidity levels look great! --- Nothing to do but maintain and wait to trim in 8 days! - [21:55]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 41% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 46% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [21:59]: Checked the 3x3 veg tent. --- Looking healthy and growth is raging! --- All mothers are looking beautiful! --- Clones are getting taller and looking healthy! --- Gorilla Goat #2 needs watering. Let’s go Ensign! - [22:03]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 4 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 230.8g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [22:23]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.7g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. --- Decided to train and super-crop both clones. --- Bent the taller branches to lower the canopy. - [22:38]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Onwards Ensign! Day 03 - Wednesday - 10/15/25 - Total Days: 185 ----------------------------------- Daily Averages ~[+01:00] - Temps: 75.6°F [24.2°C] - Humidity: 63.8% - VPD: 1.09 kPa - Soil %: [48, 34, 35, 56, 44, 43, 44, 42] ----------------------------------- - [+01:33]: Quick night check on the 4x4 tent. --- Looking great in the dry tent! --- Also starting to smell dank! --- 7 more days until the big trim! - [+01:37]: Checked the 3x3 veg tent. --- Veg garden is healthy and growth is green! --- Both the Heady OG BX1 #1 and TKO mothers will need another topping soon. --- Clones in the propagation tray also look great and no need to water. --- The explant media is still looking sterile with no signs of contamination. --- Easy night in the garden. Nice! - [+01:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Okay! Tomorrow plan to dive back into tissue culture and prep more growth media. --- Onwards Ensign! Day 04 - Thursday - 10/16/25 - Total Days: 186 ----------------------------------- Daily Averages ~[+01:00] - Temps: 74.6°F [23.7°C] - Humidity: 62.2% - VPD: 1.11 kPa - Soil %: [46, 34, 35, 56, 43, 44, 44, 45] ----------------------------------- - [11:41]: Alright! Back to tissue culture and time to start another batch of establishment media! --- Took the plant growth regulators out of cold-storage (my fridge). ------ [ » SMOKES A BOWL « ] --- While the products warmed up, gathered the necessary supplies and setup my workstations. --- Currently, focusing on: ------ PHASE 01 » ESTABLISHMENT --------- » DAY 01 » MEDIA - PART 01 - Mixing the growth media --------- » DAY 01 » MEDIA - PART 02 - Sterilization of media and tools [via autoclaving] --- Going to use the same establishment formula, but doubling the yield. --- Also using RO water instead of distilled water. --- Here's the establishment media formula: --- [» VER.0001 |» INITIAL TROPICAL PLANTS FORMULA |» 0.5L (500mL) RO WATER «] ------ MS [Muashige & Skoog] --------- » 2.22g ------ Sucrose [Cane Sugar] --------- » 15g ------ BAP [6-Benzlaminopurine | Cytokinin] --------- » 0.5mg » 0.5mL » 500μL ------ IBA [Indole-3-Butyric Acid | Auxin] --------- » 0.05mg » 0.05mL » 50μL ------ AGAR [Jelling Agent] --------- » 3.5g ------ PH [Potential Hydrogen] --------- » 5.7 --- [» 1mg/mL CONCENTRATION ratio | 1mg = 1mL | 1mL = 1000μL «] - [12:59]: Time to prep the growth media! --- Made sure to turn OFF the lung-room's oscillating fan. --- Filled a measuring cup with 400mL of RO water. --- Dropped a clean magnetic mixing bar into the measuring cup. - [13:05]: Placed the measuring cup on the magnetic stirrer. --- Turned the unit ON and have the unit on LOW. - [13:11]: Using a precision scale (sensitive to 0.01g) along with a micro-pipette (1000μL capacity along with tips) and disposable (100mL) weigh boats, ------ Added 2.22g of MS [Muashige & Skoog] ------ Added 15g of Sucrose [Cane Sugar] ------ Added 500μL of BAP [6-Benzlaminopurine | Cytokinin] ------ Added 50μL of IBA [Indole-3-Butyric Acid | Auxin] ------ Added 3.5g of AGAR [Jelling Agent] ------ Then filled the measuring cup to the 500mL line before covering the top with plastic wrap. --- Alright! - [13:46]: Let the media mix on the magnetic stirrer for a bit. --- Have the unit set to LOW with a steady vortex. ------ [ » ~20 MINUTES LATER « ] - [14:09]: Okay! Time to pH the establishment media! --- Gently removed the bottom cap of the AC Infinity PH Meter PRO [AC-PHM7]. Placed the cap in the storage case for later. Covered the top with plastic wrap (KCL solution still inside the cap). --- Swirled the probe in a cup of RO water to rinse off the storage solution. --- Shook the excess water out and wiped the unit with a clean paper towel making sure all mineral buildup was removed (kept away from the very tip of the probe). --- Temporarily placed the meter back into the cup of RO water. --- Removed the plastic wrap of the establishment media. - [14:13]: Using the AC Infinity PH Meter PRO [AC-PHM7], --- Took the establishment media's initial pH reading. --- The pH was at 5.14 --- Using the micro pipette, ------ Added pH Up solution. --- Made sure to let the media solution mix for a bit before checking for pH readings. - [14:21]: Adjusted the media's pH until it stabilized to 5.58 --- For this batch, tried to go for a range of 5.4 to 5.8 pH. --- Also glad that I did not need to add PH down. - [14:43]: Poured the pH'd establishment media into separate polypropylene containers. --- Made sure to re-mix the media solution between container fillings. --- Ended up splitting the media solution into 12 containers. ------ 8x » 125mL Polypropylene Bottles [with screw cap lids] ------ 4x » 236mL (0.5pt) Glass Mason Jars [with polypropylene jamming lids] --- Have the lids of all vessels loosely on and set each aside for later autoclaving. --- Also filled 12 empty polypropylene bottles with RO water and placed the lids on loosely (4 bottles are re-used). - [14:58]: Prepped the tools for sterilization. --- Took a new 5.25in x 10in sterilization pouch and added the following tools: ------ 3x stainless steel forceps ------ 3x stainless steel scissors --- Before placing into the pouch, wiped each tool with a paper towel sprayed with 70% isopropyl alcohol. --- Sealed the bag and marked the date. --- Time to autoclave everything in the pressure cooker! --- Filled a Presto 23qt-Pressure Cooker with 12 cups of RO water. - [15:06]: Added a few aluminum foil balls to the bottom of the pressure cooker. --- Placed the first rack on top of the foil. --- Made sure the rack was level and stable resting above the water. --- Gently placed the containers of media into the pressure cooker and onto the first rack. Lids are still loose. --- Made sure to give space for each container and kept them away from the sides of the pressure cooker. --- Placed a second pressure cooker rack on top of the containers of media. --- Placed the containers of RO water onto the second rack. Lids are still loose. --- Placed the third rack on top of the containers of water. - [15:16]: Lastly, placed the sterilization bag of tools on top of the third rack. --- Took the pressure cooker lid and made sure the release vent was clear of debris before placing it on. --- Sealed and secured the lid. --- Pressure vessel has been sealed! --- Alright! Time to sterilize! --- While keeping the vessel level and straight up, moved the sealed pressure cooker to the kitchen for heating. - [15:23]: Placed the pressure cooker onto a butane-gas stove. --- Turned the stove ON and set it to HIGH. ------ [ » ~20 MINUTES LATER « ] --- Once steam was venting out of the release vent, --- Placed the metal rocker onto the vent. --- Shortly afterwards, the pressure top-lock sprung up to indicate the vessel was under pressure. --- Then the pressure gauge started to rise. ------ [ » ~10 MINUTES LATER « ] --- At 15psi, the metal rocker started to rock! - [15:56]: Turned down the stove down to LOW. --- Set a timer for 15 minutes and began monitoring the pressure gauge. --- Maintained the 15psi by making minor adjustments to the stove. ------ [ » ~15 MINUTES LATER « ] - [16:12]: Turned the stove completely OFF and waited until the pressure cooker's top-lock dropped down which indicated that pressure levels were safe to open. ------ [ » ~40 MINUTES LATER « ] --- Once the pressure cooker was at safe levels (0psi) and the top-lock was down, - [16:48]: Moved the pressure cooker into the lung room. --- The pressure cooker was still quite hot so was careful to keep the vessel straight up and level during transport. --- Used towels for insulation. --- Rested the hot pressure cooker onto a stack of 5-gallon buckets covered with layers of towels and folded paper bags. --- Gently opened the pressure cooker and set the lid aside. - [16:57]: While wearing nylon gloves, sprayed my hands with 70% isopropyl alcohol. --- Gently removed the bag of sterile tools placed it onto a Pyrex dish. --- Removed the top rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle of sterile water before placing onto the Pyrex dish. --- Removed the middle rack and set it aside. --- Then one container at a time, --- Gently tightened and secured the lids of each bottle and jar of sterile media before placing onto the Pyrex dish. --- Throughout the entire process, kept the gloves on and sprayed my hands regularly with 70% isopropyl alcohol. --- Decided to store all the bottles in the Pyrex dish. - [17:16]: Have the dish resting on a shelf in the lung room. The jars of media are set right next to the Pyrex dish on a towel. --- To prevent dust, covered everything with towels. --- Done for now and will check on the growth media tomorrow. --- If all goes well, should be taking new explant cuttings in a couple days! - [17:39]: Night check on the 3x3 veg tent. --- Looking beautiful and healthy in the veg garden! --- All moms are getting bushy and getting pretty close to the grow light. --- Since I plan to take explant cuttings soon, not going to top or train quite yet. --- Clones also looking healthy and seem unaffected by the super-cropping. Nice! - [17:57]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 45% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 44% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [17:59]: Checked the 4x4 main tent. --- Ca-Caw!! I got Headies! --- Wow! Heady OG BX1 #1 is drying along beautifully and she is smelling dank! --- Also no signs of mold and RH levels look stable! --- 6 more days until the big trim! - [18:11]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Still gots more to do! --- Let’s go! Let’s grow! --- Onwards Ensign! Day 05 - Friday - 10/17/25 - Total Days: 187 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.0°F [24.4°C] - Humidity: 62.0% - VPD: 1.16 kPa - Soil %: [48, 34, 35, 55, 44, 44, 44, 42] ----------------------------------- - [22:57]: Night check on the 4x4 tent. --- Ca-Caw! Headies is smelling dank! --- RH levels are getting a bit dry, but are okay. --- Wow buds are frosty with trichomes! --- With 5 days until trim day, getting stoked! - [23:04]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 47% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 46% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:11]: Checked the 3x3 veg tent. --- Looking healthy in the veg garden! --- Mothers are getting bushy and tall! --- Super-cropped and trained the taller branches down to lower the canopy. --- Plan to take tissue culture cuttings tomorrow so glad there are plenty of tops to choose. Still deciding on which mother to preserve next. --- Clones in the propagation tray look healthy! --- The original Garlic Budder #2 clone needs watering. - [23:26]: Here is the starting initial watering range: ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Garlic Budder OG#2 » 258.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:40]: Took the saturated weight after watering, ------ Garlic Budder OG#2 » [000.0]g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [23:45]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 10/18/25 - Total Days: 188 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.8°F [24.9°C] - Humidity: 60.3% - VPD: 1.25 kPa - Soil %: [47, 34, 35, 55, 44, 44, 44, 47] ----------------------------------- - [15:19]: Decided to decrease the VPD level a bit. --- Within the AC Infinity App, --- Changed the schedule name from 'Flower [12/12]' to 'Drying [0/24].' Oops. --- For the Main Tent [4x4] controller, --- Adjusted the 'Drying [0/24]' Schedule for the exhaust fan. ------ Decreased the VPD LOW trigger value from 1.5kPa to 1.4kPa --- Adjusted the 'Drying [0/24]' Schedule for the humidifier. ------ Decreased the VPD target value from 1.5kPa to 1.4kPa --- This should increase the relative humidity a bit and will maintain a 60-65% range - [16:59]: Evening check on the 3x3 veg tent. --- Wow the veg garden is raging with healthy growth! --- Even with the lower veg temps, growth has been perky and bushy! --- The TKO mother has been looking a bit on the purple-side, but I plan to increase the temps back after trim day. - [17:23]: Okay! Time to continue the tissue culture journey! --- Checked on the sterilized media, water, and tools. --- Ooo yea! The growth media in the glass jars have solidified nicely! --- The media in the bottles also look great! Found only one that did not gel properly. --- Hmm. The sterile water bottles are not looking sterile. Unlike the first batch (using distilled water), each RO bottle had tiny white particles floating in the water. --- I think this was due to the water source. The RO water used was from the plant reservoir. For the next sterilization, will try using water straight from the tap. Will discard the entire batch and try again later. - [17:34]: Anyways! Time to cut and sterilize another batch of explant cuttings. --- Using the similar protocols as last time, but with a few changes. --- Instead of distilled, using reverse-osmosis (RO) water. --- Also simplifying the workflow and will not be using the sterile water. --- Only taking cuttings from one cultivar. --- Today, attempting to preserve Heady OG BX1 #1 --- Unlike the last attempt, going to be cutting apical nodes and will leave all leaf growth on. --- Last attempt was more for monsteras and not cannabis. - [17:35]: Made sure the lung room's oscillating fan was OFF during the entire process. --- Put on a fresh pair of nitrile gloves. --- Okay! Let's grow Ensign! - [17:47]: Filled a glass beaker with 180mL of RO water. --- Using a micro-pipette, ------ Added 20mL of Clorox bleach --- Mixed the solution. - [17:52]: Poured the bleach solution into a glass mason jar. ------ Added 1 drop of Dawn dish soap. --- Dropped a magnetic mixing rod into the jar before setting it onto the magnetic stirrer. --- Turned the unit ON and set it to a low setting. --- Placed a polypropylene lid loosely on. - [18:02]: Rinsed and filled the glass beaker with 70% isopropyl alcohol. --- Filled another glass beaker with RO water before adding a drop of Dawn dish soap. Mixed the solution. --- Okay! Time to take the cuttings! --- Using a clean pair of trimmers, - [18:09]: Gently cut two clone cuttings from the Heady OG BX1 #1 mother. --- Stripped each cutting down to 2-inch apical nodes making sure to leave about 0.5 inch of stem growth for planting. ------ Prepared a total of 2 nodal cuttings. --- Dropped the nodal cuttings into the cup of soapy water. --- Swirled the cuttings in the soapy water for a bit. --- Opened the jar containing the 10% bleach solution. --- Using clean tweezers, --- Took each cutting out of the RO water and carefully submerged it into the beaker of 70% isopropyl alcohol for about 30 seconds. --- Then transferred the cutting from the isopropyl alcohol straight into the jar of bleach solution. --- Once both cuttings were in the jar, sealed the top with the polypropylene lid. - [18:23]: Flipped and set the entire bleach jar upside down onto the magnetic stirrer. --- Started using the magnetic stirrer. --- But the cuttings were getting hit by the bar so ended up turning the unit OFF. --- Instead, made sure to swirled the bottle every few minutes to agitate the cuttings. --- For a proper sterilization, the cuttings should be agitated in the hypochlorite (bleach) solution for at least 20 minutes. --- While waiting for the explants to sterilize, prepped the still air box. - [18:39]: Unfolded and placed a Safloga still-air box onto my desk. --- Thoroughly misted the inside of the air box with 70% isopropyl alcohol making sure to coat the entire chamber. --- Then isopropyl misted and placed the following items inside of the still air box, ------ x1 » Jar of Establishment Media ------ x1 » Bottle of Establishment Media ------ x1 » Glass-Bead Sterilizer ------ x1 » Roll of Plastic Wrap [pre-cut] ------ x1 » Sterilization Bag Containing: --------- x3 » Stainless Steel Forceps --------- x3 » Stainless Steel Scissors --- Made sure to fully coat the outside of the tool bag and each of the containers with 70% alcohol. --- The bag had a torn corner (Oops), so made sure to spray the inside with a few mists of isopropyl alcohol. --- For the glass-bead sterilizer, ------ Wiped the entire unit with isopropyl alcohol. ------ Before filling the unit with the included beads, misted the heating chamber with a spray of isopropyl alcohol. ------ Misted the glass beads again with a spray of isopropyl alcohol. --- Set the cleaned glass-bead sterilizer into the still air box. - [19:02]: Still air box fully prepped for explants! --- Okay! Explant nodal sterilization complete! --- While still wearing gloves, --- Misted the outside of the explant jar with 70% isopropyl alcohol before placing it into the still-air box. --- Turned the glass-bead sterilizer ON. --- Made sure to re-seal the chamber. - [19:05]: Okay! Finally time to start the tissue culture transplant! --- Let's go Ensign! --- Sprayed my gloved hands with 70% isopropyl alcohol (also put on a pair of cloth arm sleeves), --- Placed my hands through the arm holes and into the still air box to begin the process. --- Started by laying out my initial workspace: ------ » Far Left Quadrant » Jar of Sterile Establishment Media ------ » Far Center » Bottle of Sterile Establishment Media ------ » Far Right Quadrant » Glass-Bead Sterilizer ------ » Mid Center » Bleach Jar with Explant Cuttings [Main Workspace] ------ » Near Left Quadrant » Empty [Trash Area] ------ » Near Right Quadrant » Bag of Sterile Tools & Roll of Plastic Wrap --- Opened the bag of tools and placed the forceps and scissors into the pre-heated glass-bead sterilizer. --- Set the bag in the trash area (near left quadrant). --- Loosened the lids of both containers of sterile media but did not remove the lids. The lids were resting on top and could easily be lifted. --- Also took lid off of the bleach jar and gently set it on the table. --- Took the jar of sterile media and set it in the center of my workspace (to the right of the bleach jar). --- Using my left hand, took a set of scissors from the glass-bead sterilizer and gently cut the bottom of the first cutting. --- Once the bottom cut was made, placed the scissors back into the glass-bead sterilizer. --- Using my left hand, lifted the lid off of the jar of sterile media and inserted the trimmed end of the cutting straight into the growth media (using my right hand). --- Covered the jar with the lid and placed the forceps back into the glass-bead sterilizer. --- Tighten the lid of the jar and set it aside. ------ [ » REPEATED THE ABOVE PROCESS FOR THE BOTTLE OF GROWTH MEDIA « ] --- Once the last cutting was transferred from the bleach jar into the sterile media, --- Turned the glass-bead sterilizer OFF. --- Cleaned up my workplace and placed the scrap stem tips and bleach jar out of the way (near left quadrant). - [19:28]: Wrapped the lid of each explant container with plastic wrap before setting it in the center of the workspace. --- Explant transplant completed! - [19:59]: Using a label printer (Brother DT410), made a set of labels for each container: ------ Heady OG BX1 #1 | 04.24.25 | Photo | Reg ------ Formula: v0001 | ExBatch: 0002 | Lot: 01 | Pod: 01 ------ Establishment Media | Sealed On: 10/18/25 --- Opened the still air box. --- Then one container at a time, took an explant out and wrapped the lid with a label. --- Set the labeled containers onto the propagation tray (in the 3x3 veg tent). - [20:07]: Done! ------ The explants are around 37 μmol/m²/s which gives a DLI of 2.40 mol/m²/d under an 18/6 light cycle. --- Will check daily for contamination. --- Ca-Caw!! Ahh yea! --- Heady OG BX1 is now officially my first true attempt at cannabis tissue culture! --- Using this new workflow, I may try reusing the TKO media. Do plan to take new cuttings soon. May even try using the liquid media. --- Shout out to microclone and Bill Graham! His 'Tissueponics' video contains tips and tricks of the trade that have straight up changed the way I think of DIY cannabis tissue culture. --- Plan to further refine my process and will continue to refine the workflow with each attempt. - [20:15]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 54% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 34% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 43% - [CH05 BL » GR7 - TKO] ------ 48% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 42% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 45% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:56]: Checked the 4x4 tent. --- Ca-Caw! Looking great my ladies! --- Humidity levels are still a bit on the dry side, but still okay. - [21:08]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Wow what a day! --- Oh yea, 4-more days until trim day!! Ca-Caw! Let’s go! Day 07 - Sunday - 10/19/25 - Total Days: 189 ----------------------------------- Daily Averages ~[+01:00] - Temps: 76.7°F [24.8°C] - Humidity: 59.4% - VPD: 1.27 kPa - Soil %: [48, 35, 35, 55, 44, 43, 44, 42] ----------------------------------- - [07:08]: Quick morning checked on the 3x3 veg tent. --- The veg garden is healthy and perky! --- All moms and clones are healthy and raging with growth! --- Both Heady OG BX1 #1 explants also look alive and green! - [19:26]: Night check on the 4x4 tent. --- Ca-Caw! We got MENDO DOPE in the trunk! --- Wow terps are getting loud and buds are drying along nicely! --- Looking frosty and coated in trichomes! --- Three more days until trim day!! - [19:33]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 46% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 35% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 43% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 47% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [19:35]: Checked the 3x3 veg tent. --- Vegging along beautifully in the garden! --- Mothers are getting taller and probably could train, but will wait until tomorrow. --- Clones and explants also look healthy and green! --- Gorilla Goat #2 needs watering! - [19:44]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was last watered 5 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 244.9g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [20:04]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 345.6g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Placed her back into the tent. - [21:41]: With a few days before the big trim, decided to sample a few buds! --- Cut and trimmed branch for sampling and wow she’s got terps! --- Buds are dense and have a gassy diesel funk! Ca-Caw!! Wow! --- Without even curing, pretty sure she’s the one! --- Plan to grow out another cycle (in clone of the same pheno), but I think I found my female for my first breeding project! - [22:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Ahh yea! Three more days until the big trim! --- Also next week will be the last for this log. --- But already stoked for the next! --- Planning on popping more seeds and continuing the pheno hunt for another female! --- Since I lost TKO this harvest cycle, plan to grow a clone cutting of the same pheno (#2). --- As for seeds, popping more regulars and going to attempt to grow some of Mr. Grow-It’s Chillout OG! --- Got a pack of F3s and am stoked to give them a try! --- Also going to sprout some beans of Dead Ops OG from Zaza Genetics! --- Chillout OG, Dead Ops OG, and another round of TKO! Let’s go! Let’s grow! --- Hunters of the dank! Ca-Caw! --- Anyways! --- Onwards to next week! [END OF WEEK 26]

2 days b4 harvest

Giorno 50 - la carenza di Fosforo e Potassio é sempre più evidente sulle foglie di “Caramel” che ha una crescita rallentata dei fiori; al contrario “Cream” sta crescendo bene e i fiori sono decisamente più grossi, non mostra carenze per il momento Giorno 51 - niente da segnalare, procede bene Giorno 52 - la resina inizia a vedersi bene sulle foglie, i fiori stanno gonfiando Giorno 53 - ho fertilizzato solo “CREAM” perché “CARAMEL” ha carenze ed é inutile mettere ancora fertilizzante, ho dato solo acqua. Invece a “CREAM” ho dato 1 litro di fert (1ml bio grow, 1,5 bio bloom e 1,5 top max mischiati in 1,5L d’acqua. L’altro mezzo litro rimasto l’ho buttato perché era troppa) Giorno 54 - sembra abbia reagito bene alla fertilizzazione “CREAM”, invece “CARAMEL” continua ad avere le foglie sempre più gialle, ma prosegue bene Giorno 55 - nulla da segnalare, l’odore é molto forte, si percepisce anche fuori dalla grow box Giorno 56 - i fiori si stanno gonfiando bene, ho dato circa 750ml di acqua a testa e defogliato la parte bassa dove c’erano tante foglie morte e secche. Ho iniziato il flush delle radici, spero di tagliare tra una quindicina di giorni

ve repotted into her final 15l auto pot. The procedure I used is as follows. Items needed.. Clay pebbles Hydro coco 60/40 clay pebbles/Coco. Silicium Flash Startrex Mycrotrex Bactrex And of course Bio tablets X2. 15l of hydro coco was mixed with 25g of Silicium Flash and 25g of Startrex. Mixed up well to form the main substrate. I filled the autopots to a depth of 5cm with clay pebbles and then layered up the amended substrate making a nice hole for my 300ml nursery pots. Into this hole I sprinkled in 5g of Mycrotrex and then dropped the seedling in. This then gets 2 biotabs pushed 5-8cm into the substrate and 1l of water mixed with 5ml of Bactrex I need to top feed the autopots for a couple of weeks so the root system can establish before I transfer to my flower room and the gravity feeding trays..🤘🏼👍🏼 I'm keeping temps at a constant 25c and lights are on 24hrs a day. Rh is sitting around 65%.it too low hence the domes but then it goes too high. I'm going to run a couple of days with the 65% but vpd over 1 is a bit high for so early.. we shall see. I'm more than happy with the purple punch. Considering when she was a sprout she grew out of her Coco plug and I found her flat on the floor...She is doing great. I'm a little concerned she is getting too much light. The others are all happy but punchy looks like she is a bit stumpy compared to the others. I've moved her to a side position in the tent so she gets a little less direct photons..

6/17 and 9/18 Were extremely hot days. Today to reached 98 with temps still currently in the 90s as I right this at 7:22pm. Watered morning came back to discovery the bags had dried out. I watered again and fed; 3tsp big bloom, 1 calmagic, 1 grow big, 1/2 microbrew, 1 tsp unsulphered molasses. 1tsp kelp me kelp you. Watered fed and lightly watered again. 6/23 Plants seem fine post BT treatment. Found a few dead moth larvae. Plants look great and I haven't seen any negative effects from BT. Weather gas continued into the 90s. I had been watering twice daily. After examining the medium I decided to not water today as I had hit them pretty hard the day before. Fed and watered same schedule except I switched out microbrew for kangoroots on 6/23. I was going to apply second application of BT but on examination I found Aphids on my smallest mendo breath. This plant is a mendo breath reveg and I've been worried about pests due to its density. I put out two aphid traps (I doubt it will do much since the are only nymphs) and sprayed with MiteX by bromide. Cottonseed, clove and garlic oil with sodium bicarb and a few others. If this doesnt work they are all getting captain Jack's.

I came back after vacation, the girl is ready for cutting, the rainy weather has started, so there is a threat of rot, you can say I managed to find just a few small pits in time and removed them and threw them away, the big and stiff flowers look fantastic:) but after a long trip I was tired, so I didn't take quality photos :( no everything else, I am very satisfied with the result I got:) its smell already lets me know that it will be good:) a lot of sugar leaves from which I will make bubble hash:) I will update the weight when it dries.

DE: In der fünften Blütewoche zeigen beide Last Dance-Phänotypen ein beeindruckendes Tempo. Der erste Phänotyp präsentiert sich extrem dank, funky und gassig – die Buds sind nun dicht, schwer und beginnen sich sichtbar zu verhärten. Die Harzproduktion explodiert regelrecht, die Trichome sind überall sichtbar und verleihen den Colas ein fast silbernes Glitzern. Der Duft ist intensiv, tief und dominant – ein schwerer Mix aus Gas, Funk und erdiger Süße. Der zweite Phänotyp bleibt weiterhin etwas kompakter, doch seine Blüten sind perfekt ausbalanciert und extrem harzreich. Das Terpenprofil erinnert nun stark an einen fruchtigen Cocktail – süß, cremig und mit einer tropischen Note, die sich von Woche zu Woche weiter entfaltet. Insgesamt eine sehr starke fünfte Blütewoche: beide Pflanzen erreichen jetzt ihre volle Ausdruckskraft. Die Terpene sind außergewöhnlich komplex und einzigartig – jede Pflanze entwickelt ihr ganz eigenes Signature-Aroma. EN: In the fifth week of flowering, both Last Dance phenotypes are showing explosive progress. The first phenotype is extremely dank, funky, and gassy – buds are dense, heavy, and starting to harden noticeably. Resin production has gone into overdrive, with trichomes covering every surface and giving the colas a silvery shine. The aroma is deep, rich, and dominant – a heavy mix of gas, funk, and earthy sweetness. The second phenotype remains more compact but perfectly balanced, with buds that are equally resinous. Its terpene profile now leans clearly toward a fruity cocktail – sweet, creamy, and tropical, becoming more intense each day. Overall, week five marks a major milestone: both plants express their full character, with extremely complex and unique terpenes that make each phenotype stand out in its own way.

Day 28 week 4 of flower plants looking good I dropped a 3L water bottle on a string which snapped a whole branch from the bottom 😳 on right plant 😭

Good day every one, The flowering is going on just perfect. New branches began to actively gain flowers. For additional stimulation of new flowers, I installed a household extra LED lamp. no problem with the PH at all , no problem with the PPM : thanks to Hesi.

Hello growers day 28 with the crystal candy and the red poison what can I say these girls have taken off in 28 days the red poison the tallest out the 4 is at 78cm and the crystal candy the tallest out the 4 is 65cm these sweetseeds genetic are banging iv grow a lot of autos and theses are by far the biggest iv got some really nice red/purple colour on the red poisons all the girls are doing very well no bad things to report if you’re looking to grow autos go to sweet seeds you won’t be disappointed big up @sweetseeds until next week be safe and happy growing ✌️

🍬🌈🍭

Gracias al equipo de AnesiaSeeds, Marshydro, XpertNutrients y Trolmaster sin ellos esto no sería posible. 💐🍁 Oracle Octane: Oracle Octane es una cepa altamente potente y con un alto contenido de THC elaborada a partir de una Blackberry Octane seleccionada, excepcionalmente rica en THC y aromática, y de la pesada Blackberry Oracle, inédita, de nuestro propio acervo genético. El desarrollo de esta carismática e impresionante variedad abarcó 8 generaciones y un enorme esfuerzo de cría. La variedad tiene un sabor intenso e inolvidable que recuerda a bayas dulces y limones frescos. Las plantas a partir de semillas crecen increíblemente hermosas y sus hojas y cogollos a menudo desarrollan tonos de rojo, morado intenso e incluso casi negro. Esta cepa vigorosa y ligeramente índica dominante tiene un tiempo de floración de unas 9 semanas, produce hojas grandes y alcanza una altura de unos 120-130 cm en el interior. 🌻🚀 Consigue aqui tus semillas: https://anesiaseeds.com/product/oracle-octane/ 💡TS-3000 + TS-1000: se usaran dos de las lámparas de la serie TS de Marshydro, para cubrir todas las necesidades de las plantas durante el ciclo de cultivo, uso las dos lámparas en floracion para llegar a toda la carpa de 1.50 x 1.50 x 1.80. https://marshydro.eu/products/mars-hydro-ts-3000-led-grow-light/ 🏠 : Marshydro 1.50 x 1.50 x 1.80, carpa 100% estanca con ventanas laterales para llegar a todos los lugares durante el grow https://marshydro.eu/products/diy-150x150x200cm-grow-tent-kit 🌬️💨 Marshydro 6inch + filtro carbon para evitar olores indeseables. https://marshydro.eu/products/ifresh-smart-6inch-filter-kits/ 💻 Trolmaster Tent-X TCS-1 como controlador de luz, optimiza tu cultivo con la última tecnología del mercado, desde donde puedes controlar todos los parametros. https://www.trolmaster.com/Products/Details/TCS-1 🍣🍦🌴 Xpert Nutrients es una empresa especializada en la producción y comercialización de fertilizantes líquidos y tierras, que garantizan excelentes cosechas y un crecimiento activo para sus plantas durante todas las fases de cultivo. Consigue aqui tus Nutrientes: https://xpertnutrients.com/es/shop/ 📆 Semana 4: Aparecieron un monton de erizos esta semana, el temporal ha hecho algo de mella en la carpa al bajar las temperaturas aunque todo sigue correcto. Continuamos con las dosis de nutrientes recomendadas por el fabricante.

Half Journey - WEST COAST OG X GELATO 41 AUTO @ GreenHouse seeds The new ones are promoseeds (they are in small pots, to take advantage of space and have some harvest between new generations (pineapple+apple * something*)

21.02.24 BT 32 Mahlzeit Buddy's 🙂 Den Lady's geht es Wunderbar Sie haben alle beide auf gehört sich zu Strecken. Jetzt geht es langsam los und die Buds Plustern sich auf. Jeden Tag bis Dienstag werden noch da und da ein paar Blätter entfernt aber ohne Sie zu stressen max 2 pro Pflanze am Tag. Es sind auch nur die Blätter die Bud's verdecken Heute gab es wieder lecker Futter 😋 Hesi Blüh Complex 5ml auf 1L Wasser , Hesi Booster 2ml auf 1L Wasser ,Hesi Phosphor + 0,4ml auf 1L Wasser und Hesi SuperVit. Alles zusammen ergab ein Super Nährstoff Trunk der Sie jetzt erst mal für 4 Tage versorgt . Skunk #1 riecht sehr stark aber das war klar und immer sehr sehr fein. Der Typische Skunk Geruch wird von einer schönen Zitronen 🍋 note abgerundet. Tangerine Sugar riecht sehr süß und fruchtig. Tricome bilden alle beide sehr gut, der Nodien Abstand der Skunk #1 ist definitiv kleiner somit gehe ich stark davon aus das sich fast ein dicker kolben zeigen wird und das an allen Haupt und Seitentriebe. Tangerine Sugar wird auch schöne Bud's zum Vorschein bringen aber halt keine durch gehende kolben 🙂 Ihre Triebe sind definitiv nicht so kräftig gewachsen wie die von der großen Schwester aber verstecken brauch Sie sich auch nicht. Mögen all eure Lady's Gesund und brächtig Wachsen und euch mit schmackhaften Stuff versorgen 😉 Danke für vorbei schauen bis zum nächsten Update✌️😊

Love to watch here growing,she need a new pot and LST,I didn't have much Time but will be done in the next days.

🗓️ 9° WEEK FLO // DAY 57-63 (from switch) // DAY 134-140 (from dry seed) 🌸 Below is a focus on the last four days of cultivation, my harvest routine: 🌸 [60° FLO] ⚡- Light: 30 cm / 250 watt; ⌛- Schedule: 12/12; 🌡️- 23° C - 65% RH average; 📑- PH 5.8 - EC 0.4; 🍔- Ripen is done after 10 day. Flush begins; 💧- 9° DWC change, 20 liters tap water in DWCs; 🌱- Phenotype #1 is showing spots on leaves, not a big issue at this point. Looking at the trichomes, phenotype #2 is ripening faster. [61° FLO] ⚡- Light: 30 cm / 250 watt; ⌛- Schedule: 12/12; 🌡️- 23° C - 65% RH average; 📑- PH 5.9 - EC 0.4; 💧- 10° DWC change, 10 liters fresh tap water in DWCs. 🌱- I cut a small branch from the #1, very frosty and shining! [62° FLO] ⌛- Schedule: 0/24; 🌙- Light off until Harvest; 🌡️- 18° C - 65% RH average; 📑- PH 5.8 - EC 0.4; 💧- 3° day flush. [63° FLO] - Harvest day ⌛- Schedule: 0/24; 🌙- 48 hours in dark done; 🌡️- 18° C - 65% RH average; 📑- PH 5.8 - EC 0.4; 💧- 4 days without nutrients in DWCs. I removed water 20 hours before the chop; 🌸- Trichomes: Phenotype #1: Milky 90% - Amber 10% / Phenotype #2: Milky 85% - Amber 15%. ✅ HARVEST - After 4 days flush and 2 days in the dark, I cut both plants on DAY 63 FLO; - Wet trim, removed fan leaves. 🔜- We are at the end and I am organizing my next two diaries, I really like this initial phase of organization and planning for the next four or five months. The "modus operandi" will be the same adopted in this cultivation, I will start with two very fast autoflowering plants and in the meantime I will give the two photoperiodic plants a long vegetative growth. I still have to decide on the strains, but I already have an idea. As far as the nutritional regime is concerned, there will be big changes, for the first time I will be using beneficial bacteria in my DWCs and anyone who has any advice on using them in this setup feel free to write to me and share some thoughts. 🆕- On week 15 I got the TrolMaster TCS-1 by @TrolMaster_Europe and I started to track my grow box data. I will add these info once I add the harvest week!

Really fun plant to grow out I got just over a quarter pound dry, I really dig the taste of this lady like a pastry of some kind with a cherry finish. Over all excellent finished product very pleased about it.

Sep 7: what a great plant. Totally love how it’s going. Flowers keep getting fatter and heavier and she’ll be done before the end of September. Sep 8: there a tree root sticking up a bit in my yard which means the plant can be tilted toward the late afternoon sun. This is helpful because the sun is getting really low to the horizon already and we’re down to 13 h of daylight. Plus now direct sun is done in my yard by about 6 pm. Indirect light is okay but you really want direct sun at all times. Videos shows use of 730 nm far red light at dusk. Just a few seconds is all it takes to set the plant into dark mode two hours faster. It’s a bloom booster because you get two bonus hours of darkness or a 26 h day. Very effective but don’t miss a night and you have to adjust the timing each night. When starting this on July 20 Civil Twilight, as listed on timeanddate.com for my location, was at 9:45 and today it was at 8:40 pm. The red light at dusk is a highly recommended trick if you have daily access to your outdoor plants. Also works indoors, of course, and on autos too. Also I think it helps ‘stabilize’ the plant by emphatically putting it into dark mode, and therefore less susceptible to stray light to cause a hermie. Have not had hermie problems in four seasons of using the red light so that is my only ‘proof’ but it is true so far, even with taking flash pics before using the red light. Sep 9: getting heavier and now more branches are weighed down and slumping against the scrog net. Nice problem to have, I know, and without the scrog net some of the branches would be breaking off now. She’s now officially too big and too top heavy to keep moving around the yard in pursuit of direct sun. Sun is much lower to horizon now and direct sun hours are dropping too. Sep 10: getting close to done and getting very top heavy. Awesome. Sep 12: raining today. Has been cool last few nights and some purple colour is just starting to show. I don’t want to overdo fertilizer near the end, but I decided she is showing K deficiency. I’m out of malted barley but I still have potassium silicate, so I used that and some cal-mag. I’m avoiding kelp and molasses or using more P at this late stage because that can make the dried bud hard to burn and that really sucks. So, potassium silicate and cal-mag it is. The potassium silicate raises the pH and it takes about the same volume of vinegar to get the pH down. A good does of acetate is likely good for the bugs in the soil too. Then a squirt of Dr Bronners soap as a surfactant and that also supplies some biodegradable carbon for the bugs in the soil. Seems to work overall and is cheap. Apologies for the large number of pics, but she’s so photogenic. 😎 #seedsman420growoff #seedsmanseeds