Love the night shots! Calmag is working. Plants are hungry!

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My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.

Out of the 4 girls I’ve transplanted two today, both white widows, I’ll do the other two Santa Marta haze’s in a weeks time when they have developed better roots, looking good, starting to flower. Bit of a slow start as seedlings due to being in hot soil, but we are well past that and cruising nicely now. 🤙🐞🙌

harvest soon day 83 - turned off the light

Esta semana bajo la temperatura muchísimo y estoy teniendo 12 grados durante la noche, de todas maneras parece que va a ser una floración muy rendidora. 30/05 se riega con 1,5 litros de agua de osmosis + 1ml/l de calmag + 0,5 gr/l de trico+. Se riega con poca agua debido al frío que está haciendo y con la intención que se seque un poco el fondo de la maceta 03/05 se riega con 2 litros de agua de osmosis inversa + calmag + monster bloom Ec:0,9

Hey now, this week went according to plan. I transplanted her to a 12.5 gallon container on Monday, and she started showing love for her new environment within 24 hours. I am still spreading out the secondaries, and will top them here in the next day or two, then there will be one set of topping left within 48-72 hours after that as she is starting to grow like a weed now that she has a nice root setup. Super excited to compare the bud growth and density with the same genetics I ran last time in a 5.4 gallon container compared to the 12.5. I hope everyone has a great halloween with more treats than tricks, unless you prefer the latter, be safe and blaze on!!!

GSC BY KANNABIA WEEK #18 March 15th-22nd Week #8 Flower This week she is looking good. Buds continue to gain weight with a tight bud structure that is covered with trichomes.

These 2 Six Shooter have really settled in now. Massive amounts of growth if you look back at last weeks diary. Both also just starting to show signs of flower. No deficiency in either which is a great way to start their first week of flower. Along with my Pinapple these will get all the under growth chopped out next week. The light i used to veg them kept the internodal space very short but it's also meant they are mega bushy and theres a lot of undergrowth that's not receiving any light and taking energy from the healthier tops. Very thirsty plants. They have taken 6 litres every 48 hours and I believe they will most likely need that every 24 hours once deep into flower. I'm excited to see just how good a strain this is........ so much so that I've germinated another 6 seeds and will be doing a full Six Shooter scrog when this grow is complete. Added 2 more photos at day 45. Starting to really fill the net out. Undergrowth has been chopped away. I'll keep spreading them out untill I run out of empty squares in the net. After that I'm at thier mercy. Loving this frow so far.

I grow in pure coco with organic nutrients

Chopped on day 77; and dried for 7 days. Plant 1: 34.8 grams dry Plant 2: 40.8 grams dry Total dry weight: 75.6 grams (2.70 Ounces) The plant is super frosty and smells very sweet. It's now in a grove bag and curing. Can't wait to do a smoke report in about a month. Hope you enjoy two song variations I made with a slideshow of the grow

Info: Unfortunately, I had to find out that my account is used for fake pages in social media. I am only active here on growdiaries. I am not on facebook instagram twitter etc All accounts except this one are fake. Have fun with the update. Flowering day 23 since time change to 12/12 h. Hey everyone 😀. It gets more beautiful from week to week :-). The buds are starting to develop well. They also get thirstier and thirstier. Another 1 g of GHSC Bio Bloom per liter of coconut was added. Otherwise, the week was poured twice with 0.8 l each time and the tent was cleaned. Have fun with the update. Stay healthy 🙏🏻 You can buy this Strain at : https://originalsensible.com/original-sensible-seeds-zkittlez~20503 Type: Zkittlez ☝️🏼 Genetics: Afghan Kush Indica x Grandaddy Purple x Grapefruit hybrid 👍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Bloom Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205W 💡💡☝️🏼 Soil : Canna Coco Professional + ☝️🏼 Fertilizer: Green House Powder Feeding ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.5 - 5.8 .

We have day 61 today, next update harvest 💪 --------------------- 👇 Week 8 (Pictures are from day 57+58 after 12/12, Trichomes pictures from day 60) - Just watering 1l / plant with calmag for the last 2 days. - PPFD at canopy height is approximately 750, with VPD around 1.3 - Harvest is around the corrner --------------------- Happy growing, and thank you for checking out my report! Your support means a lot to me! 🙏 --------------------- I appreciate every like and comment 👍👊😃

Lindo crecimiento 3 semana de floracion debo comentar que tienen muy poco olor creo que se fue perdiendo porque estoy seguro en la semana 5 vegetacion era un olor muy fuerte no se si es uno de los fertilizantes que bajo el olor comienzo a usar PK de B.A.C

Germinated 2 seeds had 50% success [still hoping the other pops up] chocolope #1 doing very well. They are in a 2'×2'×4' grow tent which they will spend rest of their lives as I'm trying my hands at cannabonsai. [Pretty calming] Lights are lowered close to dome to reduce stretch. Let me know what u think

Another week! 😬 A little defoliation does not kill anyone 😑

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And this is how we successfully open week 9 - After fermenting a dose of PK compost tea at the end of last week, I poured it extensively. As always, he did them very well. They also need significantly more water again. I have now also increased the humidity a bit, because there is currently drier indoor air in the room of the cultivation area. Let's see how long I leave my Sweet Seeds ladies. I'll decide that at short notice. Sour Jealousy will then be able to enjoy all the space to bloom at her disposal. Both Sweet Seeds now only get purified and aerated water and only enriched with enzymes. It is now time to signal to both to use up their reserves. After all, their genetically pre-programmed life cycle is significantly shorter than that of sativa-dominant strains - for example, Sour Jealousy from Fast Buds. She is only now really starting to flower, while the indica dominant strains are moving towards the glorious end. I have also already been able to positively determine how practical and thought through to the end 2 Sanlight panels really make sense. I am not primarily concerned with more power through the lighting, but also and above all, I am concerned with the homogeneous illumination at 11 degrees inclined positioning of the solar panels. And to have the opportunity to meet the different growing conditions individually by adjusting the heights of both panels. Of course, I will be able to strive for much higher performance in the large Homebox Ambient with CO 2 and both lamps, but area coverage is the be-all and end-all for me. Lamps currently operate at 60% each. That's definitely enough. Because 2 x EVO 4 - 80 means to me: Precision in the form of light - a tool that you learn to use. Everything before didn't convince and satisfy me nearly as impressively as Sanlight. Also the advantage of being able to operate your lighting system from the outside, via Bluetooth, yes that is really very important, practical and innovative. It makes things so much easier... Sweet Seeds - brand new: „Jealousy Z XL“ I can’t wait to give it a try next run… Super noble presentation, in my opinion. There are many reasons for an emotional attachment to a product. But one stands out in particular: the desire to design, personalize, individualize something or - in other words - the desire to own something unique and feel special as a result. Scientists have found that our buying behavior is shaped by emotions. A person does not buy a product, but an emotional feeling. Many thanks to Sweet Seeds! 🙏 I also feel the feeling that Dark Devil gives me - as a noble high. I associate the turn with a noble, very effective and multifaceted indica dominant weed from Sweet Seeds. Creative work only works in the 1st part of the turn - haha, because then the coma phase begins with subsequent binge eating! But even during sports I was in the vaporizer with Dark Devil and it supported me mightily during training. Maybe it depends on the activity at the time of consumption, haha I find it very fruity in taste and smell. Very sweet terpene spectrum. And not very top-heavy. A wonderful body high. Worries and fears - as if blown away… Some here on growdiaries describe meticulously exactly how they give what, when and how much of it to the plant, etc. I prefer to keep a diary for my passion in a different way. With passion and feeling. I feel my plants. And talk to them. They also listen to chilled music. The Kobe beef in Japan is even massaged to classical music. ;-)) I think such information is only useful for a run to those who do it exactly the same way, under the same conditions with almost the same phenotype. That is almost impossible. And not necessary either. I have to learn to read plants. Their signs are pointing. Become sensitive to it. If I know why and how something happens, then I also know how to keep something like this alive. And what it takes. And why. So let's see what we have to do today. In any case, Lady Jealousy is being driven to the hairdresser today. Yes, we're doing beauty and wellness spa today, haha. She's so busy with branching, I'll give her a helping hand. She has developed incredibly. And has an unusually long breath. Comparative: She looks like a farm animal to me. In other words, what it was bred for. Very strong and healthy adult. Then bloom efficiently and productively. And that's the fascinating thing for me. The breeder creates all parameters here to perfection. Even though I don't particularly like the marketing of Fast Buds anymore, I have to admit without envy: "I've never experienced that before." The phenotypes are absolute warriors, from another planet. Marketing is debatable. No problem. But I can only marvel at these plants and their quality. Unfortunately, too much turnover often spoils the core vision of a successful company. …PK is on the way! Addendum about the one, Lady Dark Devil 😈, which had to be harvested too early: And yet: Dark Devil gives me a deep inner satisfaction in my existence. Nothing upsets me. Nothing can disturb me, because I am one, with myself and my environment. I can only feel gratitude for that. It gives me this broad, generous sense of inner peace. For me, inner peace means balance, equanimity, even-mindedness, harmony, and stability. And that's exactly what a noble special effect means to me. When a weed variety can develop medicinal qualities. When THC simply has a more complex effect on my organism, than just the usual power high. And if, a plant that hasn't even had the opportunity to reach its full potential, can do that, it has passed all the requirements of a cannabis variation, with flying colors. And on top of that, she seems incredibly graceful and elegantly feminine to me. It's just something very special: She's a Sweet Seed! Oh I see, I'm writing a real diary here... 😜 But I love that. I love grow diaries. Look at the brave girl. She forgives me for topping, training, defoliating and in the end: that I have to kill her. But I know then for sure, that she knows, that I really loved her. And the fascinating homogeneity. Totally instructive. It behaves like a photoperiod phenotype, unbelievable but true! on 14.09.2024 I moved her to the grow room with the others. So it's almost 9 weeks. I'm curious to see if she holds out. But I think so. It seems incredibly energetic and relatively relaxed to me. I'm mentally very busy with her right now. Every morning I comb through her leaf picture. Make impressions of the smallest changes. I currently have Pk boosters or tea with her every third day, alternating. And in between only purified water with enzymes. Dark Devil and Big Devil I let slowly starve and then die of thirst. They consumed the last booster 4 days ago. She (Sour Jealousy) currently swallows 2.5 liters per day. That's really a lot. And in the morning she wants to have something again. I think the lamps tickle out their full potential. I meticulously defoliated her and now she stands happily and despite all the exertions, like a star in the sky. My star. 🌟 Sour Jealousy is really crazy, I can say that. And the coolest highlight will be: Lady Jealousy will soon be able to enjoy the whole place in the sun for the "grande finale". The smell of the two Sweet Seeds totally upsets me! I would like to bite into it. Its killing me softly. Very few leaves have a few dark small spots or dots at the tips of the leaves. (Sour Jealousy) Many thanks to BerrySweetHighhat. I think the same. Will probably be calcium. She gets this from the Bio PK Booster. I keep calm and don’t panic, it’s organic. ;-)) Sour Jealousy: Today I cleaned out small shoots again & defoliated them from below up. I think there were still too many young little shoots. Now everything should be distributed quite well. We'll see. Tomorrow I will probably harvest both Sweet Seeds. Hello everybody! Today it's Dark and Big Devil's attack. It's about time. Lady Jealousy will be allowed to use the full 0.64 sqm of cultivation area for herself from today. Take care & bee 🐝 positive 🐝… to bee continued.

Grow Your Own! Ripper seeds Is my favorite seedbank and amazing strain from limited edition seeds! Sunset Sherbet x Purple Punch its best one strain...

Well folks we chopped the Last of the Strawberry Blast 😁 The Strawberry Banana 🍌 Strawberry Blast Series 🍓 🍓 🍓 🍓 was a real blast , ive now done 5 Strawberry Cultivars and I'm extremely happy with all the results 😀 Killer Stuff 😀 I want to thank all my growmies for stopping in and showing your support 🙏 it's been much appreciated 🙏 Well my friends that's it for me for awhile😌 So until next time my friends 👉 Happy Growing and of course 🙂 Happy Smoking ........ Later Growmies 👊👊 be well 👌 FC4800 from MarsHydro Lights being readjusted and chart updated .........I've added a RU45 to the mix www.marshydro.ca 👉I am using Agrogardens for nutrients for my grows and welcome anyone to give them a try .👈 👉 www.agrogardens.com 👈 Agrogardens Cal MAG Agrogardens Grow A B Agrogardens Bloom A B Agrogardens Bud Booster Agrogardens PK13/14 I GOT MULTIPLE DIARIES ON THE GO 😱 please check them out 😎 👉THANKS FOR TAKING THE TIME TO GO OVER MY DIARIES 👈 Would you like to hang with the growdiary community 👉 https://discord.gg/gr4cHGDpdb 👈