2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Bien pues ya tenemos en marcha el 2º seguimiento del perfil... Son 6 white truffles, 6 Apple&Bazzokies de GBSTRAINS y 5 GG4 de GBSEEDS que se empezaron a germinar el 15 de mayo por la tarde sobre las 19:00 Todas nutridas con BOOM nutrients jejeje Todas las semanas iremos viendo los cambios y la evolución, los riegos y las flores que vendrán :) El 16 de mayo del 23 sobre las 07:00 de la mañana pase las semillas del vaso con agua, a los tapers con servilleta. Ahí estuvieron 24 horas mas haciendo un total de 36 horas germinando para después, pasarlas a las macetas de 1 litro. 22/05/23 actualizado, meto unas fotos mas de como van las semillas después de 7 días desde que se empezaron a germinar en el vaso de agua. A día 22/05/23 llevamos 7 días desde que se empezaron a germinar. ACTUALIZACION DE ESTADO: 30/05/23 Subo unas fotos después de 8 días. Llevamos 15 días desde que se empezó a germinar las semillas en vaso de agua. A partir de aquí, empiezo a contar semanas de crecimiento. Trasplantaremos a maceta definitivas de 7L dentro de una semana o 2.

_____📅 Week 8 | 📅 Day 50 - 56 | 17.06 - 23.06 ____________________________________ 🌼🌸🌺Pre-flower🌼🌸🌺 23.06.25 | Day 56🌞💧 🔸This week I wanted to stress my sweetys as little as possible, so only a few photos from the tent. They are standing perfectly straight and I didn't want to break or snap anything off. They are doing great. 🔸I tried out my additional light this week and connected it to my controller. As soon as the flowering starts I will switch it on. I'll have to see how much distance I use this time, the lamps were too close to the plants during the last grow “Runtz” and that didn't look nice. 🔸Next week I will start giving her Big Bud as soon as the first buds appear. 🔸Poison Envy: Would say the stretch is not over yet, very slight hairs on the nodes can be seen...it's definitely all ladies :D She is now over 35 Cm so in a week she will be 10 CM stretch, let's see when she is ready. _________________________________________________________________________________________ current conditions: 🌡️🔆= 24° 🌡️🌜= 18-19° 💨 Hum. = 65% 🔦 PPFD = 600 umol 12/12 🔦⌚DLI = ~ 25 _________________________________________________________________________________________ equipment to use: 💡 Light: 2 x Sanlight Evo 4- 120 on 60% ⛺ 120 x120 x 180 🍯 Pots: 18 liter pot 🌱 Soil: Bio-Bizz light mix 💊 Nutrients: Advanced Nutrients 💧 Water: Tap Water 0,5 EC _________________________________________________________________________________________

I have been super excited with everything up to this point . I have made all my preparations that I think need to be made and did some trial runs on my environment. I starting preparing around the 15th of January and my set up was complete around the 19th of February. I spaced my purchases out based on budget constraints. I wanted a cheap and efficient grow area. Some thing I paid more for because through all the research I did I felt quality was more important. If you are as excited as I was, you get pretty anxious when awaiting order status when you first order your seeds. You should remember you are ordering from an online seed bank not amazon. Most work Monday - Friday and are in a complete different time zone. I ordered my seeds from seed supreme Seedbank. I placed my order on 31 January and mailed money as directed. This was the scariest part for me especially when I didn’t hear anything back. I finally received a email on the 12th if February saying my invoice was paid and then subsequently mailed out on the 15th and I received on the 20th. One thing I would like to note that recommend to anyone mail cash is to make sure you spend the extra cash and track your payment. I’m currently germinating all five GSC I received I placed them in water for the first 24 hours and then a paper towel for approximately 40 Hours. I’ll add links to most of the items I purchased below. Some items I purchased through offer up and let it go app to save money. VIVOSUN 48" X48" X 80" Hydroponic... https://www.amazon.com/dp/B07GNBCVPZ?ref=ppx_pop_mob_ap_share LED Grow Light 1000W Full... https://www.amazon.com/dp/B074MBP32M?ref=ppx_pop_mob_ap_share AcuRite 00613 Humidity Monitor... https://www.amazon.com/dp/B0013BKDO8?ref=ppx_pop_mob_ap_share Hydrofarm Grow!T Premium Coco... https://www.amazon.com/dp/B01DA4PZI4?ref=ppx_pop_mob_ap_share iPower LGTESTSOIL 3 in 1 Soil... https://www.amazon.com/dp/B075LRY5M7?ref=ppx_pop_mob_ap_share Unco Industries (WWSB15LB) Wiggle... https://www.amazon.com/dp/B00062KQ42?ref=ppx_pop_mob_ap_share General Hydroponics pH Control Kit https://www.amazon.com/dp/B000BNKWZY?ref=ppx_pop_mob_ap_share VIVOSUN Air Filtration Kit: 4... https://www.amazon.com/dp/B06XFRNPR8?ref=ppx_pop_mob_ap_share VIVOSUN pH & TDS Meter Combo, 0.05ph High Accuracy Pen Type pH Meter & +/- 2% Readout Accuracy 3-in-1 TDS EC Temperature Meter https://www.amazon.com/dp/B06XKMH86J/ref=cm_sw_r_cp_api_i_2xyCCbK4MKNN4 Botanicare CAL-MAG Plus Plant Supplement 2-0-0 Formula, 1 Quart https://www.amazon.com/dp/B004JKBMRW/ref=cm_sw_r_cp_api_i_LyyCCbY8SMFTK Century 24 Hour Plug-in Mechanical Timer Grounded https://www.amazon.com/dp/B00MVFF59S/ref=cm_sw_r_cp_api_i_ozyCCbHG8Q2PB Day 1 - 2/23/19: I planted my seeds today about a fingernail length down with tap root pointed and gave a light watering with plain tap water at a PH of 5.8. Day 2 - 2/24/19: Nothing new to report no activity on the surface, misted watering at 5.8 PH. Day 3 - 2/25/19: Plant number 1 which had the longest tap root broke the surface I started a time lapse on it to cover the first moments of birth 😭 daddy is already so proud lmao. I have another light watering today with my humidity domes on, the intent is to have the plants roots work to get the required water and hopefully promote root growth. I also added 1ml of Rapid start and diluted into 1 gal. Day 4 - 2/26/19: Checked the status this morning prior to work and two more have reached the surface only two more remaining which I suspect will reach by the time I make it home from work. Misted this morning pot was looking pretty dry with the exception of the humidity domes, which I removed on all the sprouted seedling. I intend on shifting to a 20 on 4 off cycle and starting my other fans. Day 5 - 2/27/19: All five plants have broke the surface. I did a normal watering this am still utilizing the dome to avoid watering on top of the plants. I lowered the light again some of the early sprouters we stretching a little more than I wanted and appeared leggy to me. Day 6 - 2/28/19: Nothing really to report today. Excited for the name reveal tomorrow. I did a light watering. I played around with my ppm meter and ec I definitely plan on using in conjunction with my first feeding this weekend. Day 7 - 3/1/19: Was watering this morning normal levels. I also mixed a new feed to be ready for them tomorrow. While I was watering # 5 fell over I accidentally tipped it with the spout. I grabbed some left over soul to help prop it up hopefully she is ok.

Time to cut this one down to dry

Hey everyone :-) This week it smells more and more sweet and fruity in the whole room 😍. There is actually not much to report, everyone is now under 12/12 :-). This is the last grow that comes in the entire diary. From now on, each plant will be added to the diary 👍. I wish you all a lot of fun watching, stay healthy 🙏🏻 and let it grow 😎👌

Start of 10th week and i decided to flush given almost 45L of clean water & been carefull with temperature of water! the laddies are looking well faded, and i hope to get a super clean medicine :) made some trim also on fan leaves to have better light penetration on lower canopy! Buds are looking dense, frosty & smelly!

@18/06 Trying to get under control the raising EC. Tomorrow I will made the res change for the final stage. EC: 1.7 PH: 6.1 @19/06 New res: 11L with 3ml/L of Flash Clean (a.k.a. Flora Kleen) Putting the plant in the new res I saw some little bugs in the container. Hope this is not a problem. I'm going to make the flush until 7-10 days for final ripening. Tomorrow I will add to the res about 2ml/L of Ripen until the end. Today parameters are: EC: 268 PH: 6,2 👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍 Lesson Learned: AVOID THE BUGS WITH THE YELLOW TREES 👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍👍 @20/06 Added 40ml of Final Part (Ripen). This res wil run until the end, before the final flush. EC: 1550 PH: 5,45 @21/06 Added 2l with 7ml of ripen and PH+. EC: 1524 PH: 5,8 @22/06 Changed res only ripen and PH+ EC: 1250 PH: 6,4 @23/06 Added only fresh water and balancing PH EC: 1354 PH: 6,27 @24/06 Added 1l with 4ml of ripen. She seems quite close to the finish EC: 1290 PH: 6,01 @25/06 Added 2l of fresh water as PH is lowed and EC is raised. EC: 1226 PH: 5,67 @25/06 h230:00 Ready for the next week, maybe the last one ? Let's see.. Good Luck to myself

Bottomed the plants on day 22 Over three weeks into flower and they’re still stretching but starting to slow down nice pistils forming I like the way she is stacking up

Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grows First, I'm just going to say I'm done with the construction of my new growroom. I put some pictures on the construction here in week one. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable theme in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 8 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. I am very excited to see how the new growroom will work and how this CBD Fast Eddy plant from Royal queen seeds will turn out. CBD Fast Eddy Automatic is combining Cheese x Juanita la Lagrimosa x Ruderalis, Fast Eddy is a rapid growing, flavourful, and CBD-rich auto strain that goes from seed to harvest in 8-9 weeks, produce generous yields, and induce a clear, mellow, and most importantly, functional high. THC: 9% CBD: High Yield Indoor : 400 - 450 gr/m2 Yield Outdoor: 80 - 130 gr/plant Height Indoor: 60 - 100 cm Height Outdoor: 80 - 120 cm Flowering: 6 - 7 weeks Harvest month: 8-9 weeks after sprouting Genetic Background: Cheese x Juanita la Lagrimosa x Ruderalis Type: Sa 50% In 40% Ru 10% Effect: Clear, Painrelief Climate: Mild ----------------------------------------------------------------------- 2017-09-25. Week 5 starts. Added a video of the girls. Gave the girls water and nutrients. The beautiful girl is 41 cm high and have grown 5 cm in 7 days. I flushed the girl yesterday with 40 liters of tap water. Im lowering the humidity in the room with a dehumidifier and try to have 50% air humidity. She looks great and starting to bud up. ---------------------------------------------------------------------- 2017-09-29. Installation of the New light rail in the grow room. Its Advanced star light V 5.0. Added a video of it.

START OF WEEK 6 DAY 35 I am not sponsored by any company, simply wanted to try out something new this grow. NUTRIENTS USED THIS WEEK Front Row AG Part A 4.2g per gallon Front Row AG Part B 2.8g per gallon Front Row AG Phoszyme .4g per gallon Front Row AG SI .5ml per gallon Front Row AG Triologic 1ml per gallon Front Row AG Clean Up .2g per gallon Another great week after the transplant to final pots. Every visible node was topped on all the plants. Some of the large fan leaves connected to the main stem were removed. Custom drip rings were made out of some 1/2 inch tube that I drilled some holes in. Each drip ring has its own shutoff due to different substrate being used. I was getting rid of some leftover stuff from past grows. This is not ideal, but it’s what I had to work with and cost no 💰 this go around. I’m hoping for a great stretch from these Mandarin Cookies. The net will probably go up at the end of the week followed by one final week before flipping the light cycle to 12/12 for flower production. I really need the height of the Cookies to catch up to the Super Lemon Haze that are growing like a maniac! I’m gonna take clones at some point…maybe. Thanks for stopping by and we’ll see you next week.

She's growing well. Maybe 1 more week in this pot then I intend to repot into a 20L airpot.

Really exploding the first week of flower. I lollypop the bottom before bud.

Let’s go day 72 from seed!!! Another great week it’s been , 2 Ogreberrys an brucy b been flushing for a week, an the 3 others will continue to get there normal feed of nutrients, they are just not quite ready so they will start getting flushed around next week!! Sweet sweet smell of Grapes And gooey frosty nugs is all I can really say, photos do no justice, some of the craziest fruitiest smelling buds I’ve ever had, Real High quality meds !! Hope you all enjoy an have an amazing productive day as well as a great week!! Peace love an positive vibes to all y’all Cheers 😶‍🌫️💨💨💨💨💨🤙🏻If there’s any questions, please ask!! 💚🙏🏻💯

well, she is already showing signs of flowering, it's a pity that she didn't get to spend another week in vegetation :) Well, that's okay, she looks healthy and beautiful to my eyes:) which makes me happy :) good luck with the growing :)

Alles prima

I am now flushing her with plain Brita filtered water PH'd with lime juice to lower her PH. I initially first flushed her with 42L of water and now I am flushing her once a day for 8 days and then I will put her in for darkness for 3 days and then I will harvest her and dry her for 9 days and then cure her for 28-48 days.

Bomb plant bro, you should grow this, its better than the Tropicana cookies

Week 3 flower before and after defol