2 x black apple hitchcock 1 x black hell 1 x choccolatto To be put into a dwc 4 pot homemade 60+ltr each pot. Also. 3 x kukulukan 1 x Indian kush To be vegging while the others bloom ready for next run. After 8+ weeks of veg

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hi liebe Community and Welcome Back! 🌿💚 Willkommen zur fünften vergangenen Blütewoche! Hier im Bio Tabs NL Grow, erreichen wir aktuell eine höhe von 139cm. Und eine richtig krasse Breite von 69cm. Deswegen, habe ich nun die Seitentriebe aufgrund von Platzgründen etwas hochgebunden. Insgesamt zeigt sich hier in diesem Grow eine stärkere Blütenausbildung, als bei der anderen Pflanze. Phenotypisch? Wir werden es noch sehen. Die Pflanze hat ihren Stretch beendet und konzentriert sich nun völlig auf die Blütenausbildung. Beide Pflanzen sind jetzt tatsächlich gleich hoch. Es zeichnet sich ein durchgängis schönes und gesundes Wachstum ab. Die Trichomen und Stigmen, bilden sich nun rasant aus. Das Supercropping bietet der Pflanze eine zusätzliche Stabilität. Das Aroma hat bei dieser Pflanze einen stärkeren süsslichen Zitrustouch. Diese Woche gab es einen Bio Tabs PK Booster Kompost Tee! Und die Lichteinstellung habe ich für die finale Blütephase nun auch hochgedreht. Die Umgebungsgegebenheiten sind aktuell gut: ————— 🌞 Temp: 26 🌚 Temp: 18°C bis 19°C 💨 RH: 56% VPD: 1,10kPa 💡ppfd: 830 mpm ————— Viele Grüße 👋

Day 57: Girls looking good and healthy. Got the HLG turned up 100% pumping 500 watt to em! We shall see. Still only given around 500ppm nutes. Day 58: Got mostly cloudy with just a couple amber it looks like. I'm thinking I need to start flushing. Albeit, only on one plant. I will wait a few days more until I make a decision. Day 59: Still looking beautiful! Buds are getting thicker! Decided I will give MOAB till week 10 and check the tric's at that time. Prob start flushing at that time. Only time will tell. Day 60: Girls looking good. 👊 Not gonna lie, getting nervous as I do not want to cut em too soon or too late. This is a tough spot. The one has a ton of red hairs. It looked as if there was just a couple of amber that I found the other day. I was looking on the outside of the buds, also. So I know the inside is where I need to look. Just not wanting to jack with them too much. I will check again at end of week. Day 61: Busy. No updates. Day 62: These girls are starting to smell amazing!! I don't see any noticeable growth here lately. I'm hoping they start to bulk even more though as I think I am still seeing some clear trichomes. Day 63: Welp, all is well and no new updates. I been feeding pretty heavy so I will back off and just water tomorrow.

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They gettin fat

This seed was massive and germinated like a dream. Good signs 🤙 Thanks for checking in 💯

Coming along

She looks extremely healthy and happy let's see how she performs at the end,she's 100% organic grown without any chemicals from bottles. Starting to show the first pistils on August 24th

End of day 48 flower and girls are super frosted. Took a sample from day 42 flower of White Widow and it was one of the best I EVER smoked with a head punch right after the smoke leaves your mouth. It was incredible and can’t imagine how she’s gonna be after harvest. Might harvest half early and half late to get the best of both worlds. Still feeding them nutes and will slowly drop down as the days count down towards harvest. Will also be turning down the daylight time from 12/12 to 11/13 to 10/14 to 9/15 to 8/16 and then 72 hrs of darkness. I plan on getting the most resin out of her as possible. End with a split stem and ice cold water.

Welcome to Green House Seeds Company Cup 🏆. Day 62 since time change to 12 / 12h The last week before the harvest has come 🎊. Hey everyone 🤗. The time has come. The last days and hours have struck for this great lady 😃. In the coming days she will move to the darkroom and spend another 48 hours there in complete darkness 😋. Of course, there will be an update before the big update after fermentation 👌. In the last update there is again a detailed description of the individual strain. I'm really looking forward to finally being able to try the strain 😄. It looks beautiful and smells extremely tasty 😍. It is a genetic perfection 👍 all shoots have grown up, it looks very symmetrical and has simply grown perfectly :-). I am also very enthusiastic about Green House fertilizers 😀👍 . @Greenhouse: Thank you so much for these genetics 😍👍. She will get a place in the mother's closet. I wish you all a lot of fun with the last update before the harvest, stay healthy 🙏🏻, and let it grow 🌱🍀 You can buy this Strain and Nutrients at : https://greenhouseseeds.nl/ ☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼☝️🏼 Green House Seeds Company Cup 🏆 Type: Wonder Pie ☝️🏼 Genetics: Wedding Cake x OG Kush 👍😍 Vega lamp: 2 x Todogrow Led Quantum Board 100 W 💡 Flower Lamp : 2 x Todogrow Led Cxb 3590 COB 3500 K 205 W 💡💡☝️🏼 Earth: Canna Bio ☝️🏼 Fertilizer: Bio Grow Feeding ( GHSC ) , Enhancer ( GHSC ) , Bio Bloom ( GHSC) ☝️🏼🌱 Water: Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 6.0

These have been on water there entire lifes I'm I am a new grower and learned the hard way have now got a full parcel of green buzz liquids on the way

4/18/25 They started a new week now so to say🙂. They are all looking wonderful, between 11 and 12 cm tall. 😍🍅🍅💚 Grow babbbbiieess😍🍅💚 4/21/25 So I transplanted them today in a soil mix with 70% tomatoes soil (store bought) and 30% old soil with perlite. Also on top of the soil i put some volcanic stone. They got their spot on the balcony now and my "little balcony garden" concept is starting to take shape. Almost done but all the plants must now just grow😊🌱💚 They got watered with plain water PHed down to about PH 6.🍅🍅❤️💚🌱 4/23/25 They are all super happy and bathing in the sun. They look strong, lush and growing. My balcony garden concept is coming slowly true. 😁😍 Grow babieees growwwwwww💚🍅😍😍

Overall I enjoyed this grow and learned a lot about living soil. Going for a slow dry, trim, then at least a one month cure. Hopefully another six days and I’ll update with a dry yield. My next batch is already cooking but with 5 gallon pots. 03/25 - Day 7 Drying B & C are done drying and have been in jars for a day now with RH ~60%. Total 3oz from B & C. Total 1.5oz from A & D

This week I start using atami bloombastic recommended by local professionals grower who always grow best quality 👌 So he advice me to use bloombastic last 4week off flower start with .5 ml per litre then increase to ml and he said last week before chop only feed her with bloombastic and water nothing else then flush for 3 or 4 days I will do that and let you guys know the final result Day 61 and orange sherbat seems start accelerate on fatten up hope she produce big nugs coz one orange sherbat is stooned and bud stay very very small (I don't take pic off her either)but can see it in grow questions

On the final few days of a 10 day flush. Will be chopping down this week to harvest and hang dry. Really excited to try this one. The smells in the room are unreal.

Deep Forest Auto is still trucking along. She is still bulking and the ph has bee. Stable all week. She should be on her way to finish soon. Thank you again Doctor's Choice, Medic Grow, and Gen1:11. 🤜🏻🤛🏻🌱❄️

Can’t get the humidity down but it’s within vpd so hope that’s okay