Start of week 4 of flower! Off to a troubling start, as the light switched on over 1 hour ealiere than scheduled. I took a spare timer, and set it up to continue it's 12/12 schedule, but now moved one hour back. I cross my fingers they forgive me for this error.🙏 👽👉31/10 The day after watering and the day my timer messed up my 12/12 schedule. But hope it brings no problems. I defoliated lightly and only removed most under the SCROG line. Even a couple of underdevoloped branches that stretched below SCROG-net got cut. 👽👉 1/11 Been having a battle with humidity after watering, but upped my air circulation to help. 👽👉 2/11 Waterday* Upped my nutes on BioHeaven, BioGrow and BioBloom. 👽👉 3/11 They seem to be responding good to the upped nutrients. 👽👉 4/11 Humidity under control. 👽👉 5/11 The VPD on point, has really speed up the trichome production. 👽👉 6/11 Waterday* Stayed on the same schedule, as last watering. They seem very satisfied and no signs of mistreatment.

All good. After 2 weeks of 12/12 girls showing nice bloom. I still train and defoliate lightly. After this week I will do stronger defoliation and let them bulk up.

Week 8 flower Average VPD of 1.14 Pheno 3-4 are smaller plants grown with AC infinity watering bases and Build A Soil in 10 gallon pots

Day 68-16/02/22 I’ve harvested 6 of the 25 so far they are drying some more to come down over the next days all buds looking juicy and crystals everywhere!!!

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

I have not been able to post for a week because I broke my tent and had to order new one. The plants sat in the dark for a week as a result. Now they have been back in my new tent for 5 days. I started today with growth nutrients with an ec of 0.7 and PH 6.2 and in a few days I will top the plants.

TH 1Q2025 - Week 8 - Flower 5 
(ON Haze X Original Haze) X Northern Lights #2 “Todd’s Haze” Objective - 8 Female Plants, Topped ONCE @ Flip, 12” when topped - Modified Sea of Green Seeds Wet: 1139PM, 28.2.2025 Germinated: 2.3.2025 Flip: 21.3.2025 Harvest: 77 Days, DATE: 6.6.2025 _________________________________________ __ Fri Apr 25, 2025 TH 1Q25 36:F:5:1 Cleaned Emitters - Replaced Pump Cleaned Manifold Filter = TWICE Replaced IRRIGATION Pump with mini-sump Photosynthesis plus produces enough biofilm to disqualify from running in the reservoir. - Remediate: Apply half diluted to watering zone, every other day. Include PCAL 1660 for Add’l Calcium and Phosphorous - [x] CEASED PHOTOSYNTHESIS PLUS IN RESERVOIR - The BIO film is ‘orange/pink,’ PSP is the SUSPECT. - [x] Harvest Dehu - [x] Refresh Reservoir - 2 Gallons - [x] EC: 2.3, 2.4 - [x] Primer A & B: [ 31, ml] - [x] Silica Skin GEN 3: [ 16, ml] - [x] SLF-100: [ 10, ml] - [x] 1900: Measure Runoff - [x] Amount: [ 1250 , ml] - [x] EC: [ 2.3, mS/cm] __ Sat Apr 26, 2025 TH 1Q25 37:F:5:2 - [x] Replace Main Feed (1/2 Silcone tubing) with 3/8” Black Chemical Resistant - [x] Refresh Reservoir - 2 Gallons - [x] EC: 2.3, 2.4 - [x] Primer A & B: [ 31, ml] - [x] Silica Skin GEN 3: [ 16, ml] - [x] SLF-100: [ 10, ml] - [x] 1900: Measure Runoff - [x] Amount: [ 950, ml] - [x] EC: [ 2.4, mS/cm] __ Sun Apr 27, 2025 TH 1Q25 38:F:5:3 - [x] Lower Defoliation in Preparation for Intra-Canopy Lighting Install - [x] Harvest Dehu: 3.5 Gallons (None Yesterday) - [x] Refresh Reservoir - 3 Gallons - [x] EC: 2.4 - [x] Primer A & B: [ 48.8, ml] - [x] Silica Skin GEN 3: [ 24.4, ml] - [x] SLF-100: [ 15, ml] RUNOFF: [ 950, ml, 2.4/5, mS/cm]  __ Mon Apr 28, 2025 TH 1Q25 39:F:5:4 Observations - @ 2.4 EC is making for DENSE GREEN in the leaves. We DON’T lack nitrogen. ;-} - Some Nitrogen Curling on NL2 Dominant (Pheno #2) - Will REDUCE EC if doesn’t abate today . . . - We have some White filmy scum forming on top surface of res water. Suspect Silica. Will clean out res TUESDAY and restart w/Out Silica Skin. If we’re clear FRIDAY - Start Re-adding until and unless white scum forms … Reducing EC: 2.3 # Will reduce Day by Day to 2.1 - [x] Harvest Dehu: 2.5 Gallons Runoff - [x] EC: [ TBD, mS/cm] - [x] Amt: [ 950, ml] __ Tue Apr 29, 2025 TH 1Q25 40:F:5:5 - [x] Install Intra-canopy Light 50% Dimmer Note: After H2O2 yesterday, and 1 cup (in about 2 gals) today - the amount of scum is REDUCED SIGNIFICANTLY. I filtered with hand strainer until there was no more film or particularate (there wasn’t a lot) - The overall appearance is better. Have refreshed with 3 Gallons and Primer A&B Only (and SLF-100). We’ll monitor. If it stays clean, we’ll test again with Silica Skin Gen 3. __ Wed Apr 30, 2025 TH 1Q25 41:F:5:6  Refresh Reservoir - [x] Amount: [ 2, Gal] - [x] Primer A&B: [ 32, ml] - [x] SLF-100: [ 10, ml] Runoff Amount: [ 2, gal] EC: [ 2.9, mS/cm] R&R Reservoir (Rinse components w/ 45% H2O2) - [x] Disconnect MAIN FEED Line - [x] Disconnect, Remove, and Clean PUMPS - [x] Flush Chiller - [x] Clean Reservoir Reassembly - [x] Reinstall components NOTE: We have a SMALL amt of white slate like precipitate - Most Likely Silica … __ Thu May 1, 2025 TH 1Q25 42:F:5:7 Mix 1 Liter of CalPhos for HAND APPLICATION Tonight CAL50K, 1 ml yields .5 EC (250 ppm)/Liter, or .125 EC per Gal ~16 ml’s/gal - ~2.1 EC - [ ] Mix and ApplyPCAL 1660 & CAL50K - [ ] Photosynthesis Plus - [ ] Quillaja 60 - [ ] Apply ~ 120 ml/plant - [x] CAL50K to EC: 2.1 (4 ml/qt) - [x] For 2.1 EC: [ 16, g] Cal50K *** RESERVOIR EMPTY!!! *** After REDUCING Per Event flow in Half - we STILL Emptied the reservoir … SINCE we ONLY HAD 2 GALLONS, We’re good (Catchment is 2 Gal) Last night I cleaned the manifold filter ~ 7PM (Start Time) and reduced to 18 minutes TOTAL time (9 Events, 2 minutes/event) Runoff Amount: [ 7600, ml] # We Emptied the Res Overnight, at 3 min/event - dropped to TWO ~2300 EC: 2.9 Refresh Reservoir: 2 Gal (Reclaimed DEHU) - [x] SLF-100: [ 10, ml] - [x] Primer A&B: [ 32, ml]

Going into week 8 she is looking great. I just added some cha ching and it looks like she doubled in frostyness over night. Its been a little longer than I thought since I last took some close up pics but I still think it shows a difference,

fast growing auto, overloaded on nutes with this one but she still produced beautiful buds

Hey everyone :-). The last plants were also placed in the bloom chamber kammer. Everyone has made great progress this week 🙏🏻. There is not much to say about this week, I think videos and pictures say more like words 🙈😎. I wish you all a good start into the week :-) Stay healthy and let it grow 🙏🏻👍

checking under my jewelers loop daily like it’s nobody’s business! All cloudy still not much is changed but I’m being patient. I did clip a couple branches off of the white runts because it seemed very dry so I took them off and trimmed them I may give them a toke later this evening. Today is the last day I’m going to water I think I’m gonna have my lights on for two more days and then do 48 of darkness I’m already one day until week 10 of flowering and I should be done any day now! rh able for following my grow!

I think she is a beautiful strain, but man is it a pain in the ass to trim here and there, and to LST. Her stems were always very fragile no matter what i did, not to break but to bend. Stayed short, and grew bushy, I couldn't get them to strengthen up. But overall I would say its pretty to look at and grows densely and might be worth a try.

Hi guys how are you ? welcome back to our enchanted gardens! We are ready and extremely excited to see these California pearls blossom in my home 😆😋 And you??? Want to know how it will end? What will be the color of their gems, their perfume... their production?... well... stay updated and follow our diaries Happy growing everyone

Chopped yesterday at day 67. 8/23/2024. Also chopped seed plant

I pour the beet by Hand. Every two days like 5%-10% of the total soil amount. Today (12.5.) is 28.Day of flower (55-70 Days total) Lamps are on 600w the top of the flowers have 600-800ppfd Vpd is 1.2-1.3

WEEK 10 - 25/9/2021 - 1/10/2021 30/9/2021 - No major issues these past week. Plant 1,2 and 3 responding really well to scrogging. The original purpose of the net was to support the buds actually. But since the net is in place, I thought it would make more sense  to use the opportunity to bring the 3 plants to the same level as plant 4. Despite that, plant 4 still has trouble catching up and plant 1-3 just stretched like crazy. Plant 1-3 have started flowering and stretching. Not plant 4 though. No signs of stigmas yet. Weird genetics. Been having issues since germination. Major defoliation done for all plants. Sucker branches removed and LST readjusted. Complete res change done. 30 gallons of water mixed with the following: Liquid Silicon - 1ml/gal - 30ml Cal-Mag - 2.5ml/gal - 75ml Micro - 3.5ml/gal - 105ml Gro - 2.5ml/gal - 75ml Bloom - 3.5ml/gal - 105ml Hydroguard - 2ml/gal - 60ml pH - 6.12 EC - 1.21 mS/cm T - 24.5

420 Fastbuds FBT2301 Week 3 Merry Christmas Grow fam. Week 3 for these beautiful plants. They seem to be progressing well with minimal issues. I did up the feeding to 1000ml every other day and so far they seem to be OK. All in all Happy Growing

Week 9 day 1 video pheno. Gonna chop this week Week 9 day 7 well chop this weekend so on week 10 Day 1-2. Just waiting on a light to reveg one. Everything is magic

clones from the seed grown plants. This is a F1 Fast strain. Photoperiod. I topped these plants that were autos. I see that topping these girls triggered something left over from the auto genetics...I have had autos on more than 1 occasion immediately start flowering. I am sure this would have had it not been a photoperiod. I have 10 clones of this and I will not be topping it until absolutely necessary. Had i not been down to 1 tent and a grow light I would have moved on with flowering those cute little things...