Lots of progress this week. Harvested my oldest purple trainwreck plant and moved the white widow into the ScrOG net in her place. Hoping for a big yield from this younger plant. The lollipopped older plant is starting to swell, I’m thinking she has about 3-4 weeks left. I harvested the mother plant on day 70 and she had a nice smooth smoke that was great for the day. Here’s to hoping for a good yield. Happy growing! 👩‍🌾🏼🌱

Удалось избавиться от жёлтых пятен, АК-47 здорово вытянулся - 27 см. Едем дальше😉

Nice increase in height this week with many beautiful new growth nodes. If i was going to Top, I'd do it here soon, but this gals gonna be mostly au natural. Still getting just the cfl bulbs for now while she's small so I can watch her closely. Already some amazing big fan leaves on her. Humboldt Seed Organization has this Blue Dream CBD labeled at a 1:1 ratio. Which can hopefully bring in a thc and cbd percentage of 10. I'll be on the lookout for smells of berries, pepper and citrus. Happy growing everyone. ✌️

End of week 4 , huge difference each week. Buds started fast, strong purple genetics showing instantly. Can't wait to see what happens. So far everyone is healthy and happy

happy with plants at this stage. cant wait to see them bulk up. they are starting to smell a little now the sour lemon tart is alot more pungent than critical orange punch at this stage.

Empieza la sexta semana. Esta debía ser la última. Pero todavía quedan unos días más, así que me voy a extender a 7 semanas. Se pueden ver algunos cogollos grandes y voluminosos. Algunos otros no tanto, pero sin tanta diferencia. Hice una solución bien cargada para aprovechar la última semana de engorde. Espero llegar cómodo a la cosecha y sacar buenos resultados. Ya fumé algunas hojas con la pipa de agua, y el sabor y potencia se empiezan a notar. Seguiré actualizando cómo se termina esto. Día 38. Todo sigue muy bien. Las flores siguen engordando y las hojas llenas de tricomas. Creo que a pesar de algunos detalles, el resultado va a ser interesante. Ya es la cuenta regresiva y de no mediar problemas habrá una cosecha abundante. Por ahora se prende intermitentemente el ventilador a la noche, por lo que no se ven problemas de humedad. Pero ante la duda en cualquier momento coy a dejar prendidos los extractores toda la noche. Día 40. Estoy viendo que unos pocos tricomas recién se empiezan a poner blancos. Por lo cual puede ser que incluso se alargue una semana más de lo que pensaba, llegando a 8. Hoy llegó el overdrive de advanced, pero no se si usarlo en esta planta, ya que depende de la maduración de los tricomas si empiezo el lavado en tres días, o no. El engorde es muy notorio en estos días, así que ya estoy bastante conforme. Aún así voy a ver si es necesaria una semana más de nutrición o no.

The progress is real! On the 14th, all seeds had germinated. The difference of 4 days is, for me, unreal. I will keep you guys updated. See you soon

14.10.2022 56 days from seedling. This beauty hasn't stretched anymore but she looks amazing. Slight increase in flowering nutes. She is consuming more amount of water in irrigation. The plant is a little dark green so I took a few more days to fertilize. The buds are gaining weight at a good pace, I can already see the size of some trichomes by eye. Good weekend to everyone. Happy growing 💚🌱🙏

D73 - not long left, bit of yellowing up but not too much worrying as I'm flushing them with pHd rainwater. Both looking like good yielders! ❤️💛💚

Las 4 nenas del scrog iniciaron con el ciclo 12/12 el día 05/11/2021: están siendo alimentadas con advanced nutrients(dosis indicadas en la foto de la tabla) -micro -blom -grow -b52 -rhino skin -big bue -bud ignitor -bud candy -sensi cal mag xtra Las 2 nenas en macetas de 30LT 18/6: por el momento están siendo alimentadas con advanced nutrients(dosis indicadas en la foto de la tabla) -micro -grow -blom -b52

August 10, 2019/Day 64/1 of Week 10: Last week, I watered minimal and had very little runoff. I have been watering every two days at a PH of 6.5. Day 65: Watered with 3L at a PH of 6.4. Runoff PH 7.01. At 9 PM, light moved to 15" and A/C regulating tent temps to 24.7/76.5F to 25.3C/77.5F. Day 68: Watered with about 2.5L at a PH of 6.43. Did not measure for runoff. Light still at 15" Day 69: Moved light to 17" above canopy End of Week 10 notes: Stop getting hair in my buds. lol Cheers to all the growers that wear hair nets, lab coats and gloves! I'm glad to see that she has very few amber trichome tips. She is mostly clear and starting to go cloudy. I want more time to beef up. Will be interesting to see how fast the trichomes continue to change and when her white pistil growth slows. 😁

Well we are going to week 8 and this is the first of the many weeks of flowering that has started .. The girl is very stinky and she smells almost citrus like .. The male flower is a great healthy looking baby 👶 I'm excited to get some pollen off of her . Keep your eyes on this one

Cut and hung, only the best buds kept, beginning dry cycle. Into jars after 11 days dry, beginning cure.

Das wars. :) Habe nun geerntet und die Pflanzen 8 Tage in der Hazelbox getrocknet. Ich habe mit den Titan Samen am 5. Oktober (Seeding) angefangen und bin praktisch am 1. Januar mit Trocknung fertig. Das ist wirklich super schnell. Die Pflanzen hatten zum Schluss ca. 60-65 cm Höhe. Die Pflanzen hatten richtig, richtig viele Blätter, was man auch beim Schneiden nochmal gemerkt hat. ;) Ich habe getrocknet 176 Gramm aus den 3 Pflanzen insgesamt. Die Buds sind richtig fest und klebrig. Es riecht sehr süßlich (bisschen in eine Bubble Gum Richtung) und die Buds glitzern sehr stark. Eine tolle Genetik. Für diesen Grow habe ich wenig Zeit und Arbeit reingesteckt. Die Erde ist vorgedüngt, das Bewässerungssystem funktioniert, die Box macht die Programme automatisch. Ich musste also nur maximal 2 mal die Woche den Tank auffüllen und ein bisschen extra gießen. Kaum Arbeit, aber ein echt solides Ergebnis in der kurzen Zeit auf so kleinen Pflanzen 

AUTO BLACK OPIUM - Another amazing week - Loads of trichomes, colours are darkening and pistols are turning amber. The smell is ridiculous - The buds are sticky and I cant wait to smoke some. AUTO GIRLS SCOUT COOKIES - She just keeps on growing larger and larger - did defoliation on her for the second time during pre flower stretch and it looks like I might have to do another. AUTO SWEET REBEL - Removed this plant from the diary as it grew incredibly small but using it as a comparison to show off the Black Opium colours.

First week of flower is over. She handled the light change and nutrient change without any issues. last bit of LST was completed and last major defoliation had also been done. From now on I will only defoliate when leaves are covering other shoots. She was already smelling strong before, but it keeps on getting stronger and stronger. Definitely not a stealthy strain in the smell department.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Pineapple Express leaves are all losing color at this point, she’s getting close. Not sure how much longer to let this one go... she’s on day 57. Online recommended harvest date says day 55-65. Trichomes appear cloudy to me already but she’s shooting new white pistils everyday. The bud that was pollinated appears to be the most done. Its sugar leaves are beginning to yellow. The other buds seem a few weeks behind this one, I’m still trying to wait so the seeds fully develop. I don’t want to chop her too soon and not have fully developed seeds. The other buds I am trying tic heck trich’s on. Berry white is chugging along still, it’s still not as far along as the others but appears to be more frosty even though she’s a small plant. Looking forward to seeing how she develops further... I suspect the buds on that plant to be very tight. Thanks for reading and happy growing! 👩‍🌾🏼 🌱