Tag 63 Blüte die 10. Woche beginnt und die Damen sind fast soweit aber für es sind immer noch zuviele Trichome klar und die ersten werden erst Bernsteinfarben. Würde ich WPFF also Waschen und Liverosin pressen wäre jetzt oder in paar Tagen Ernte. So wie es aussieht dauert es also noch ca 10 Tage. Meiner Erfahrung nach sind jetzt die entscheidenden Tage wo noch viele sekundäre Stoffe wie Terpene und Cannabinoide geildet werden. Der Duft ist Fantastisch bei jedem öffnen des Zeltes. Da der Sommer mit der Hitze eine Pause macht kann ich niedrigere Temp. halten und schone noch die ganzen flüchtigen Terpene. Also ich habe wirklich viel Glück mit dem Wetter gerade. Die letzten Tage habe ich angefangen nur noch mit Wasser zu gießen, angepasst mit Canna CalMag auf (EC 0,5)(PH 6,0-6,2). Nur der mineralisch gedüngten Frozen Tropicana Cherry mische ich noch 2ml/L Canna Flush ins Gießwasser um Salze rauszuspülen. Den Drain halte ich gering. Es reicht mir wenn ich nur 5% drain habe (der EC ist da bei 1,4 und PH 6,1). Besser könnte ich es mir nicht wünschen. Canna Terra hat wieder einmal mehr als perfekt funktioniert. Die Ladies trinken auch noch viel auch das zeigt mir das Sie noch wollen und es noch zu früh wäre sie zu ernten. Jeder grower kennt das ja am Ende trinken sie nur noch sehr langsam und sagen dir ja Buchstäblich das es soweit ist. Ich finde es sehr wichtig die Pflanzen zu beobachten und nicht irgendeinem Plan stur zu folgen. Da wir in paar Tagen in Urlaub fahren muss ich wohl die Ernte und das aufhängen der Pflanze meinem alten Growbro anvertrauen genauso die Vorzucht des neuen grows. Ich hoffe das die Damen noch nicht trocken sind wenn ich wieder da bin. Aber da will ich hier keinen zulabern. Ihr könnt euch ja vorstellen wie das ist erade in den letzten Tagen nicht da zu sein. Zum Glück kann ich mich da auf meinen Kumpel verlassen er hat auch schon viele Jahre Erfahrung und ja meinen Grow Style ja von mir gelernt und fast 1 zu 1 übernommen.. Ich hoffe die Fotos und meine ersten Videos gefallen euch. Sagt mir gerne ob ich weiterhin Videos aufnehmen soll oder interessiert euch das weniger. Ich wünsche euch allen gesunde und glückliche Pflanzen und ein schnes Wochenende

Hey y'all - this is the exciting one for me, this year - I'm submitting the buds from these plants to the 4Plants Cannabis Cup in November, wish me luck! I'm in the amateur medical category, growing one of my favourite strains, it's exciting! Hope you enjoy the video, I'm just a doofy guy growing some dope, hoping it's, um, dope! Thanks for looking, let me know if you have any questions or comments 🙏

** HARVEST DAY FOR PHENO2: 09-27-2022 ** Pictures are from weeks 11~17. Flowering was a breeze, the girls were pretty much on cruise control, I only had to water and feed them every week or so. Notes of spicy orange, really pungent smell. Both are very similar in that regard, one being more spicy and the other more fruity.

Day 12 of organic grow and all is well. Seemed to have dialed in the conditions and able to keep good air movement, CO2 levels, and consistent temperatures/humidity with the ThruWall Closet Fan (Model TW108 ), and the Holmes Twin Exhaust/Intake Fan (Model HAWF-2041). Humidity is maintained with a Lovoit 6L Ultrasonic Programmable Cool Mist Humidifier (Model# LV600HH). Added a SCROG screen (just a cargo net) and bought the Sample Pack of organic nutrients from Nectar for the Gods (just pay shipping and they will send you the sample nutrients - check out the website). People say their line of nutrients make the buds smell and taste like no other! I have always been an organic Earth Juice nutrient guy, so I am going to experiment with combining the two. Lights are on 24/0 and she has tight internodal spacing with good vertical growth. Room that closet is in is beginning to smell skunky at times, and like diesel at other times. Will begin light organic nutrients with next watering. If you made it this far, please check out my other autoflower grows. Day 13 - Watered today with very low strength nutrients added. - Zannabis7

Day in and exit fan 15 min off 15 min on Van is full on Night in and exit fan 30 min off 15 min on Van is off Lst tranings return Day 35 Short Flowering 2gr/1L 1Liter Per Pot Day 40 Short Flowering 2gr/1L Booster 1gr/1L 1Liter Per Pot

hi friends farm, did you have a good Easter? we made a decent harvest that left me quite satisfied even if I had to collect a little earlier because of my absence at home but this did not ruin the work 🤪🤪🤪🤪😎😎

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Had to move plant to a bigger PC I believe the dimensions where approx. 18"x 22"x 9"

Einfach eine super Woche Ich bin einfach wieder komplett überzeugt davon autoflower indoor anzubauen, dank fastbuds gibt's so geile Sorten. Ich will am ende alle mal gegrowed haben

Day 16-27/12/21 doing well!!! Day 20-31/12/21 plant is looking good!!!! Waiting to move half of them to another tent!!!!

This week I noticed a good and healthy growth. I decided to Top one of the plants and on another one aI applied the “main line”. On the third plant from the next week I will try an “LST”. The temperature on the grow box is under control and umidity is good. The only problem we found was on the “Tangie n.2” that is developing quite slowly and showed up some yellow leafs. 20/03 we apply LTS on tangie 3 and 4.

Here are week two looking lovely

Flipped to Flower this week. First day of 12-12 light cycle was 10/04/21 Did some defoliating half way though the week. Kept tucking taller shoots under the scrog net. Also installed a dehumidifier near the end of the week becuase I couldn't get the humidity to go below 65%.

Week 9: Navigating the Path to Harvest! Greetings once again, fellow cultivators! As we journey deeper into the heart of our botanical adventure, Week 9 brings us closer to the long-awaited harvest of our beloved Epsilon F1. In this week's installment, we dive into the intricacies of nutrient management and the final stages of flower development. Our Epsilon F1 stands tall and proud, a testament to the care and dedication lavished upon her throughout her growth journey. With each passing week, her beauty becomes more radiant, her buds more plump and resinous. It's a sight to behold, a testament to the magic of nature and the art of cultivation. Ah, but this week brings a significant shift in our approach to feeding. We've made the bold decision to cut all additional nutrients, relying solely on Fluvic Blast and Enzym+, both trusted companions from the Aptus Holland lineup. Why, you ask? Because at this stage of the game, our focus shifts from nutrient uptake to nutrient utilization. Fluvic Blast, with its rich blend of organic acids and trace minerals, acts as a catalyst for nutrient absorption, ensuring that our Epsilon F1 can make the most of the resources available to her. Enzym+, on the other hand, plays a crucial role in breaking down organic matter, releasing valuable nutrients that might otherwise remain locked away in the soil. Together, these two powerhouses work in harmony to optimize nutrient availability and promote healthy, vigorous growth in the final weeks before harvest. As we approach the culmination of our journey, let us not forget the lessons learned along the way. From the delicate balance of light and nutrients to the art of patience and perseverance, each day in the grow room has been a lesson in humility and gratitude. We owe a debt of gratitude to Royal Queen Seeds for providing us with the genetic masterpiece that is the Epsilon F1, and to Aptus Holland for crafting nutrients that have nourished our plant from seedling to harvest. And so, as we bid adieu to Week 9 and prepare for the final stretch, let us take a moment to marvel at the beauty of our Epsilon F1 and the miracle of nature's creation. May her harvest be abundant, her buds potent, and her legacy enduring. Until next time, dear friends, happy growing! #EpsilonF1HarvestJourney #Week9FinalStretch #BotanicalBeauty #RoyalQueenSeedsGenetics #AptusHollandNourishment Genetics -Epsilon F1 @rqs_esp @royalqueenseedssp @rqsglobal Food - @aptusholland @aptus_world @aptus_es @aptus_portugal @aptusbrasil @aptusplanttechaus @aptus_thailand @aptusplanttechnz @aptususa_official LED @lumatekeu Controls - @trolmaster.eu @trolmaster.agro @trolmaster.support As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life With true love comes happiness , Always believe in your self and always do things expecting nothing and with an open heart , be a giver the universe will give back to you in ways you could not even imagine so #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #rqs #ApoloMission #MoonHarvestAdventure #playwithlego #lego #legotime #legovideo #tothemoonandback More info and complete updates from all my adventures can be found Link in the profile description Friendly reminder all you see here is pure research and for educational purposes only Growers Love To you All

She took of very quick , loves to Be bent and played with doesn’t need much water, very bushy, buds densed up very quickly beautiful tasty smoke , recommended to everyone , please buy this strain over at @royalqueenseedsqpss

Started slow but now is going well💚

Decided to add another two a week later Just to complicate things a bit 😂. This will run beside diary 1. Germination was fast. this will be first RQS grow experience. never had a bad seed from PEV yet. alone.

She is growing amazing! Buds are sticky af and the small is really interesting, love it! Hope to hatvest her in 1-2 weeks

She is exactly 3 weeks old, its already time for her to start some trainings ^^ let’s see how she will react. humidity still remains as a problem