She is tiny but strong.

Awesome ! Big hard and jucy headbud 🙌 the smell is very delicus !! I don’t know the genetic about this plant ‘cause i found it in some weed gifted to me but it’s incredible only 73 day from germination it ready to harvest 💪🏻💪🏻💪🏻🌲

983 gramme sec sur 10 clones en table a marée EBB and flow de 1 M2 sous une lampe a 750 watt ce qui donne 1,31 gramme par watt par mètre carré 😯👌😁

Update 2/4 (day 25): * Turned the lights back up to 100%. The top of the canopy is getting 50K Lux and they seem to be enjoying it. * Very small signs of flowering in the two smaller plants. Nothing yet from Pineapple Express #1, which was surprising since it's older. * Trimming bigger fan leaves to leave a clear top and bottom to the canopy. * Trying to train the colas to an even distribution and without getting too close to the sides. I am going all in on fewer-but-larger colas to try to boost the harvest. I hope I don't learn a hard lesson by not letting side branching to occur but want to give it a shot.

First feeding. Going well so far fist day of last. Lst going well.

The autos are all doing pretty good, some minor deficiencies from early on but seem to be doing much better now since I got the new bottle of calmag. They probly have another 3 weeks left I'm guessing they are fattening up nicely tho.

Flower time! Cant wait to see how she comes out. I have bent the main stem over a couple squares to keep her a little lower and spread out to be part of the scrog without topping her. Seems to have worked great and all the main colas are above the canopy but not too tall the are gonna block the surrounding ladies. She nice and snug in there making herself at home. I am expecting some nice and plump flowers from her as she looks very healthy. The root supplement and the folair feeding does seem to have helped a bit but i'm always cautious for placebo. The weights at the end will tell the tale. I already have quality down so I'm looking to raise the weight without sacrificing quality. I would also like to improve on my smell and flavors, have been steadily improving crop to crop and hopefully will keep that trend going for a long long time. Just a little better each crop with small techniques that add up to fat flowers!! She kinda blends in this week but the next week or two she will stand out again with her unique flowers

***Sponsored Grow*** = Medic || https://medicgrow.com || Grow = ***Sponsored Grow*** First ever CBG Grow, all thanks to @SeedStockersOfficial / @Seedstockers I asked them for some sample seeds ages ago and they responded in kind, they let me pick out a few strains and sent some others, this was one of the ones I picked. I also received from them their CBD 1:1 Silver Lime Haze Auto, CBD Northern Lights, Blackberry Gum Auto & O.G. Kush Auto, really looking forward to the sliver lime haze and blackberry gum. Might grow these ones out door this year for fun, autos seem to do better then photoperiods for me on my deck. As of now I've scarified the seed, soaked in water for 20hr then moved to paper towel till a 1mm or less tap root was showing then planted in the medium. This grow has a lot of new things happening. its my first attempt at indoor growing using dry crystal fertilizers, the brand of nutrients I'm using is Lotus. It was also a gift from another user @SanderanderS as shipping to them would have been astronomical for the stuff they wanted. I have noticed some weird things like boated packaging, off gassing and weird consistency with the nutrient line up so far and been in contact with the reps online about it, They said everything is normal and not to worry. Another new thing with this grow is how it will be grown under the new LED's by Medic Grow. They will be under two of their MINI-SUN 2 240W LED grow lights using their V1 Spectrum, Its good from seed to harvest. You can find more information about this in the foot note. Just going to wait for another week till It pops up and will update as follows. Watered in with PH of 6.2 and a EC of 0.6 or 340ppm 500 scale. This is part of a 5 part diary in conjunction with medic grow led lights, in the beginning it was to be 6 plants but one of the selected strains died out and its replacement/backup seeds also failed. Sorry to say Humboldt Seeds Raspberry Diesel was a big flop all 3 seed did not meet my expectations, really wanted to try that one it looked nice. Can add that to the failed 4 seeds of Seedsman Cookies Chill :( ***Sponsored Grow*** Official Website: https://medicgrow.com/ + https://seedstockers.com/ Facebook: https://www.facebook.com/medicgrowled + https://www.facebook.com/SeedstockersBarcelona/ Twitter: https://twitter.com/medicgrow + https://twitter.com/seedstockersb Instagram: https://www.instagram.com/medicgrow420/ + https://www.instagram.com/seedstockers_seeds YouTube: https://www.youtube.com/channel/UCNmiY4F9z94u-8eGj7R1CSQ + https://www.youtube.com/channel/UC4GiFYrIWeNtwtXjcivCmfw Growdiaries: https://growdiaries.com/grower/medicgrowled https://growdiaries.com/grow-lights/medic-grow + https://growdiaries.com/grower/seedstockersofficial https://growdiaries.com/seedbank/seedstockers

This week has been great ,today is day 78 and the girls are just doin their thang , 1 looks ready for flush already and the rest look about another longer then flush for them aswell,, can’t wait to see how these girls finish out over the next couple of weeks 😍!! Y’all keep them eyes peeled an stay tuned for next week! Peace love an positive vibes to y’all Cheers.💨💨💨

Week 6 and I'm back to normal nutrients regimen. Frost and size is increasing. No issues with anything and they're pretty much on cruise control 😎

Okay, that's it, I couldn't watch her suffering like that anymore. Her trichomes could've gone a little longer, but her overall appearance clearly was over it. The lack of aroma and stickiness from the trichomes worries me, but I do know how much she struggled during flower and that the nutrient availability in the soil was off, so I'm not really surprised. I've downgraded my expectations for the end result quite a bit over the last couple of weeks to a more realistic level considering the circumstances. For drying I'm trying something new - I've got a wine chiller, a poor man's Cannatrol, that I want to keep the temps low during drying in summer and for curing and storing in general. Curious to see what effect this has on the dried buds.

Hacemos domingo 4 mayo te aireado y echamos 22 gr de top dress

"Chugging" along this week. Weather is hot, girls are loving the all time drip system. Forgot that my nutes and hot sun dont mix well and created a bad case of algae. Will monitor more often and maybe increase the drip flow

7/20 Went over this morning to plants soaking wet and heavy rain. Went back around 4pm and plants actually look really good. The rain has stopped for a bit and the girls looked great. Apart from some septoria leaves I needed to defoliate on the first plant I treated. I also noticed a few others on other plants so it's spreading albeit slowly. I treated the three in the middle with their second dose of Plant Doctor at 3tsp/1gal. One gallon administered to each plant via root drench. I think I may treat the other plant showing signs with a diy Dr. Zymes using citric acid as the active ingredient. That way I Gould start treatment right away without having to rely fully on plant doctor. Plus it would kill any pests. Ive seen damage but the birds do a number on them. I dont want to spray my plants with a bunch of chemicals if I don't have too. I shouldve replaced the pallets and cleaned the cage better. I think I did a good job sanitizing as I have no wpm. Just this septoria I probably got when the lady mowing the lawn cut all tjis fucking grass and blew it in my pen, throwing bird seed and I'm sure tons of disease into my grow bags. I literally had to harvest a bunch if fucking sunflowers. It looked like my plants had been mulched. Just with small disease carrying shrubs. At some point some of this stuff has to be on purpose. You CAN'T be that stupid. A couple kushes in the back look hungry. I think I may need to up my feed. I've written to much. I'll keep this updated. Thanks if you made it this far. 7/21 I guess it rained last night. Oh well. It was blue skies for hours before dark. It must've rained late/early morning. I'm sure the app of plant doctor will be fine. Like I said I'm considering using citric acid as a foliar spray to help with the septoria and clean up any pests. I don't have enough pests to warrant spraying. I just don't want to put all my eggs in the plant doctor basket. Today is overcast and windy. The wind has really assisted in spreading this virus. I remove anything I see infected immediately and since these plants are reveges they have plenty of leaves. Plants are really stretching. I know I talk about the septoria a lot but realistically if someone looked through my garden they'd have a hard time spotting it. I don't want to exaggerate the problem but I don't like dealing with ANY fungus or molds. 7/22 It was 55° this morning when I went over. I defoliated some septoria leaves and a few that were fading or damaged. I saw almost a whole leaf eaten so it's probably a good idea to move forward with citric acid. I can add that to the plants I've already treated with plant doctor. This is the first year it's been so noticeable. It's the reveg 10th planet that started this off. Oh well. I've dealt with MUCH worse. Plants LOOK good (other than septoria leaves (on bottom of plant). Today is water day for me but it's been raining and it's a lot cooler today. Bags still had weight to them. I want them to have a proper wet dry cycle. Temps top out at 75° so I'll monitor during the day and if something NEEDS WATER I'll give it to it. I'm suprised I have ZERO WPM. The septoria is much more identifiable but pm is usual present in these conditions bur I don't have a spot of it. I may do some defoliation today. I also need to add supports for flowering but one thing at a time.

Week 18 (26-5 to 1-6) 26-5 Temps: 19.5 to 24 degrees Humidity: 44% to 58% Watering: Both 1000 ml. EC: 1.3 27-5 Temps: 19.8 to 25.9 degrees Humidity: 48% to 58% Watering: Both 1000 ml. EC: 0.3 28-5 Temps: 20.4 to 25.9 degrees Humidity: 49% to 60% Light set from 75% to 70% strength. 29-5 Temps: 20.5 to 26.2 degrees Humidity: 52% to 59% Watering: Both 1000 ml. EC: 0.3 30-5 Temps: 21.8 to 26 degrees Humidity: 54% to 62% Watering #1: 1000 ml. #2: 750 ml. EC: 0.3 Light set from 70% to 60% strength. 31-5 Temps: 22.1 to 25.9 degrees Humidity: 52% to 60% Watering: Both 1000 ml. EC: 0.0 1-6 Temps: 20.5 to 24.9 degrees Humidity: 49% to 61% Watering: Both 1000 ml. EC: 0.0 Will harvest next week!

They all doin pretty good, leaves are a little too yellow for my liking at this point I should have added some grow to the mix but they will be fine I think. This one is another one of the shorter ones with fat buds. It has a huge main cola and is maturing at a more rapid clip than everything else except the purple one. REMEMBER , IF YOUR SHOPPING FOR GEAR YOU CAN USE THE CODE “BANGDANG” FOR 10% OFF YOUR ENTIRE PURCHASE FROM ANY OF THE FOLLOWING COMPANIES. @greenbuzzliquids @rainscience_growbags @gorilla_grow_tent @growlightscience.led NEW* @Rocbudinc Seeds on his website * *****Gorilla grow tent discounts extend to all companies affiliated with grow strong industries which include..***** @super.closet Lotus Nutrients Kind LED grow lights

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

I'm a little late with update, I was very busy. In grow room it is all under control. the buds are filling up. Strech has stoped. There is 18 days since flower Smeel is pretty strong, i just need to set up carbon filter. Next update will be in a fev days. ✌️🤘💚💜