2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

For sure i will keep this plant in stock. Very different and potent head high with great looking buds and strong flavor.

ya se ven las flores, riego cada tres días , y corto las hojas que están caídas solo .último día de la semana 9 ya se aprecian las flores

420 Fastbuds Gelato Auto Week 4 Hello fellow growmie's this week has been awesome for growth. I would say the two girls probably grown 6in in the past week and recovered very well from the mild defoliation I did a week prior. I still only feed just 2 liters of well water daily and they both show no signs of any nute deficiencies. It still has been super hot with Temps in the high 90's but over all keeping plenty of air movement seems to be working good. All in all Happy Growing.

Stacking time!

Den 63 od přepnutí.

Day 49. I seem to have misplaced some of the photos I had so I will be uploading them when I find them. My carbon filter decided to fall off and land on the front right plant which caused it to lose a main branch. Some slight issues with some lower leaves as you can see at the end of the video . Still full blast on bio canna range and molasses. They will be switched to 12/12 today.

floweeer start day 104 change fertilizer from nettle to chicken manure, from my free run chicken yard

Hello guys! So it’s the end of week 2 of flowering and all is going well. Been watering every 2 days and, as you can see, we have white pistils and small buds in every plant Not much to say, the ladys are green, stretching and growing buds as it should Yesterday i’ve defoliated one of the critical + 2.0 clone and next week I’m going to defoliate the rest of the plants. Just wanted to test when it is better to do the defoliation so it’s kind of an experiment Tell me what do you think of how this is going

Everything is movin along some will be coming down this week a few need a little longer. I'll get some pics up a little later today I have to retak these pics tmm they got deleted Retook the pics on friday so they are a couple days late but wasnt much different than 2 days before

Steady away for her. Cut nutes down a touch getting some burn on her. Hasn't had any sumo boost either. She's doing good though, hopefully see her fatten up. Done better than I thought as it is anyway 😁

Semana tranquila, lo de siempre en estas últimas semanas , ir atando apicales como buenamente se puede para que no toquen el techo del invernadero. el resto son fotos de lo que le fui añadiendo al invernadero para favorecer la ventilación y la renovación del aire. De esta forma me puedo permitir cerrar la puerta hasta abajo y que quede todo más protegido.

Day 15 Mon PH 5.8 EC 0.6 PPM 300 DLI 19h PPFD Water 20 Day 16 Tue PH 5.8 EC 0.6 PPM 300 DLI 19h PPFD Water 20 Day 17 PH 5.8 - EC 0.6 - PPM 300 - DLI 19h PPFD Water 20 Day 18 Thu Add A&B 2.5 mll PH 5.9 - 5.7 EC 0.6 - 0.8 PPM 300 - 400 DLI 19h PPFD Water 20 Day 19 Fri PH 5.7 EC 0.8 PPM 400 DLI 19h PPFD Water 20 Day 20 Sat Add Ph Up PH 5.4 - 5.8 EC 0.8 PPM 410 - 420 DLI 19h PPFD Water 22 c Day 21 Sun PH 5.9 EC 0.8 PPM 420 DLI 19h PPFD Water 21c

Hey there growers!! Sorry for skipping flower so bad.. here are some videos of the ninth and tenth week. No excuses, but someone stole from me back then and I didn't feel like making videos for a couple of weeks. But the experience went on, the female plant reversed and it's pollinating itself with my help like you see in the videos. Sorry again for the Spanish audio 😅

Think these will be done by end of week 8 possibly, adding UV + IR for terpes and pushing for an earlier finish! 🍋

Guys had some hard weeks imagine all cookies seeds becomes ladyboys chopped all. Lost about 9 weeks. My whole hope finish him perfectly only 3 weeks left 💀💀

Week 8 begins for LSD and Green Crack. These ladies are getting close to the end, there was a lot of yellowing leaves on each plant, so they were removed. Not much else to say. Thanks for stopping by growfessors 👽🌳💚

Helloo, planties are roughtly 3 week into vegetative growth. Into second week our cat snucked into the tent and being the motivated gardener she is, dug out quick one and speedy chille, we put them both back into place gave planties some water, and with time they recovered, speedy chille got most stress, but being photoperiod, recovered slowly aswell, still the shortest and very loved. :) so everythings good Royal kush has the strongest smell of them all, few leafs we defoliated we grind in our hard and smell a deep green canna scent. Northen lights is the leader in hight since begining When planties grew 5 tops we topped them, began doing LST and a bit of defoliation, bended stems away from the lamp and taking off leaf that blocks light reaching tops. The stems hardened and remind me now more of a tree

Week went again very uneventful. The plant does what she is supposed to be doing (gaining weight) :). Still lots of new pistils appearing overnight, I am checking the Trichomes daily, however, everything remains milky, with only very little % of amber trichomes. Once amber colours start increasing I will start flushing.

Eternity Grow Cup 2025 with Plagron and Zamnesia 🌱 Runtz - Zamnesia Seeds It’s now week 11 since germination and week 7 of flowering, and our beauty is thriving! She’s loving the nutrients from Plagron, which has given her everything she needs to grow into a strong, healthy plant. We decided to take a small bud from the middle branch to try it out, and wow—it's covered in trichomes, glistening like frost. Plagron's nutrients have truly made a difference, enhancing both the size and resin production of the buds. The result speaks for itself—dense, aromatic, and full of potency. We can't wait to taste the magic! 🏆🏆🏆🔥🔥🔥