Today I moved two plants to their final pot (3.5 gal). Each of the plants has 2 nicely formed nodes. The root structure looked very strong. They were started in Pro-Mix HP and transplanted into the same medium. I just sprayed PH balanced water on the top of the soil to help them acclimate to the new pot. I still have 2 plants that have stopped growing so I am going to leave them in the seedling chamber till they get a little farther along.
You should start lst when the plant has 3 to 4 nodes if about don’t lst a overwatered/underwatered plant you want to train healthy plant especially autos can set them back happy growing
Everything here is looking good friend. I would suggest trying to get the lights closer to your plants & maximize the PAR they are getting for as much growth as possible. If you do not have them already get yourself a PAR meter & thermal temp gun to read the plant tops. It will really help you get the most out of your lights. Please feel free to follow my profile & never hesitate to ask me if you have any questions. Happy growing!