Siam’s Smile

1. The workbench and all gears were cleaned with water. Then sprayed and wiped with 99% isopropyl alcohol to sterile. 2. A 400ml of 0.5% H2O2 solution was made by diluting 4ml of 50% food grade H2O2 with 396ml of Reverse Osmosis (RO) water. 3. Seed was submerged into 15ml of 0.5% H2O2 solution in a corresponding glass jar for 12 hours to soften its shell and prevent it from bacterial attack. 4. A Root Riot cube was also submerged into 0.5% H2O2, but in separate container. It was submerged for 12 hours to sterile. ===== 12 HOURS LATER (12/13/23 11:16 PM) ===== 5. Germinating temperature is recorded at 21.7c. RH is irrelevant since a humidity dome will be used. 6. Root Riot cube is picked out off the H2O2 bath and drained off excess liquid by shaking (!!!NEVER SQUEEZE THE CUBE!!!). 7. Seed is sowed ~0.5cm into the opening hole of Root Riot cube. 8. The cube is placed into the tray and labeled along with two other seeds. 9. Humidity dome is put on with all ventilation holes closed. ===== 72 HOURS LATER (12/16/23 10:56 AM) ===== 10. Seed reoriented itself. Good sign. Expect a sprout within 24 hours from now. ===== 86 HOURS LATER (12/17/23 01:20 AM) ===== 11. Young sprout is supplied with 120 µmol/m2/s of light from two T5 LEDs under 24/0 schedule. ===== 95 HOURS LATER (12/17/23 10:35 AM) ===== 12. Humidity dome is opened 10%.