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CalGonJim 9/2 1 pm getting ready for my all the darkness Halloween 25 grow dark devil auto by sweet seeds are pretty amazing. This batch is from 2021 all the seeds from sweet seats feel like they just came out of the mountains are incredibly strong!! and I’m going to try to not kill a banana purple punch I don’t know what it is. I’m a banana purple punch serial killer. I’m gonna get one of these right someday maybe Halloween will be that day or something like that🧛‍♂️🧛‍♂️🧛‍♂️🧛‍♂️🎃🎃🎃🎃🎃🎃💀👻 9/4 634am Chemical Seed Priming: Molecules and Mechanisms for Enhancing Plant Germination, Growth, and Stress Tolerance: https://pmc.ncbi.nlm.nih.gov/articles/PMC11941364/. (google Ai) which amino acids are good for seed priming? Amino acids effective for seed priming include Proline, Glutamine, Tryptophan, Arginine, and Gamma-aminobutyric acid (GABA). These amino acids can improve germination, enhance seedling growth, increase photosynthetic pigments, and boost tolerance to abiotic stresses like salinity and low temperatures by promoting energy metabolism and nitrogen content. Specific Amino Acids and Their Benefits Proline: Improves root architecture and nitrogen content in rice seedlings, enhances mineral uptake and photosynthesis in canola, and plays a vital role in seed metabolism.Glutamine: Priming with glutamine in onions leads to better germination rates and enhanced photosynthetic pigments.Tryptophan: A precursor to vital compounds, it significantly promotes germination and seedling emergence, especially under salt stress.Arginine: Promotes seed energy metabolism, which is crucial for increasing germination rates at low temperatures, particularly in wheat.Gamma-aminobutyric acid (GABA): A non-protein amino acid that primes plants to respond faster to stress by inducing a sensitization state. It also interacts with stress-related hormones like abscisic acid and ethylene. How Amino Acids Help Stress Tolerance: Amino acids can help plants withstand abiotic stresses such as salinity and low temperatures.Energy & Photosynthesis: Arginine and proline improve seed energy metabolism and photosynthetic activity, respectively, supporting better growth.Growth & Development: Proline improves root development and overall growth, while glutamine can enhance photosynthetic pigments.Metabolic Precursors: Tryptophan serves as a precursor for various beneficial biologically active compounds, contributing to overall plant health and development. AI responses may include mistakes. [1] https://www.sciencedirect.com/science/article/pii/S1672630816300324[2] https://www.researchgate.net/publication/367000964_Seed_Priming_with_Exogenous_Amino_Acids_Improves_Germination_Rates_and_Enhances_Photosynthetic_Pigments_of_Onion_Seedlings_Allium_cepa_L[3] https://www.sciencedirect.com/science/article/pii/S2214514124001284[4] https://www.mdpi.com/2311-7524/9/1/80?type=check_update&version=1[5] https://www.sciencedirect.com/science/article/abs/pii/S1878818121000943 "" 9/4 7am Grok: Seed priming is a pre-sowing technique where seeds are treated to enhance germination, seedling vigor, and stress tolerance. Using amino acids in seed priming can improve these outcomes by supporting metabolic processes, stress resistance, and early growth, which is particularly relevant for your autoflower cannabis grow (e.g., your **Blueberry KO** cross) documented on GrowDiaries. Given your innovative nutrient mixes (e.g., TMG, glutathione, proline, glycine, glutamine) and interest in optimizing autoflower cultivation, I’ll focus on amino acids suitable for priming cannabis seeds, their benefits, and how they align with your research goals. I’ll provide a concise overview of effective amino acids, their mechanisms, application methods, and considerations for your grow, ensuring compatibility with your existing regimen (e.g., Connoisseur Bloom A/B, FloraKleen). ### Why Use Amino Acids for Seed Priming? Amino acids act as biostimulants in seed priming by: - **Enhancing Germination**: They provide readily available nitrogen and support enzyme activation for faster, uniform sprouting. - **Boosting Stress Tolerance**: Amino acids like proline and glutathione protect seeds/seedlings from oxidative stress (e.g., high light, temperature swings), crucial for autoflowers’ rapid growth. - **Improving Vigor**: They promote protein synthesis, root development, and nutrient uptake, giving seedlings a strong start. - **Cannabis Relevance**: For autoflowers, priming with amino acids can accelerate early growth, maximizing the short vegetative phase (2–4 weeks) and supporting your research into stress resilience and terpene enhancement. ### Effective Amino Acids for Seed Priming Based on agricultural research and cannabis cultivation insights (‽web:11, ‽web:12, ‽web:13), the following amino acids are effective for seed priming, with a focus on cannabis: 1. **Proline**: - **Role**: An osmoprotectant and antioxidant, proline accumulates under stress to protect cells and enhance germination. - **Benefits for Cannabis Seeds**: - Improves germination rate and uniformity under stress (e.g., high humidity, temperature fluctuations), common in indoor cannabis setups. - Enhances root development, critical for autoflowers’ fast cycle. - Aligns with your use of proline (200 mg/L) in foliar/root mixes, showing your familiarity with its stress-protective effects. - **Concentration**: 50–200 mg/L (0.05–0.2 mM) for soaking solutions (‽web:11). - **Evidence**: Studies show proline priming improves seedling vigor in crops like wheat and rice under stress, applicable to cannabis (‽web:12). 2. **Glutathione**: - **Role**: A tripeptide (glutamate, cysteine, glycine) antioxidant that reduces oxidative stress and supports metabolic activity. - **Benefits for Cannabis Seeds**: - Protects against oxidative damage during germination, especially in high-light or nutrient-heavy environments (like your hydro/coco setup). - Enhances seedling resilience, supporting your GrowDiaries research with Setria glutathione (125 mg/L) in foliar/root mixes. - May improve early terpene synthesis pathways, aligning with your FloraNectar use for flavor. - **Concentration**: 50–150 mg/L, as higher doses may be less effective for root uptake (your current 125 mg/L is suitable). - **Evidence**: Glutathione priming enhances germination in crops like mung bean; its use in cannabis is experimental but promising given your foliar success (‽web:11). 3. **Glycine**: - **Role**: Supports protein synthesis, chlorophyll production, and nutrient chelation, boosting early seedling growth. - **Benefits for Cannabis Seeds**: - Enhances photosynthesis and root development, critical for autoflowers’ short vegetative phase. - Improves nutrient uptake, though it may chelate calcium/iron (as noted in your prior questions), requiring monitoring. - Familiar from your mixes (500 mg/L), making it a natural fit for priming. - **Concentration**: 100–500 mg/L, aligning with your current use (‽web:13). - **Evidence**: Glycine priming improves seedling vigor in maize and soybean, relevant for cannabis. 4. **Glutamine**: - **Role**: A nitrogen transport amino acid, supporting chlorophyll synthesis and early growth. - **Benefits for Cannabis Seeds**: - Provides readily available nitrogen, speeding germination and leaf development in autoflowers. - Enhances stress tolerance via glutathione synthesis, synergizing with your Setria glutathione use. - Used in your mixes (50 mg/L), showing your experience with its benefits. - **Concentration**: 50–200 mg/L to avoid excessive nitrogen, which can inhibit germination (‽web:11). - **Evidence**: Glutamine priming boosts germination in rice and tomato, applicable to cannabis. 5. **Arginine**: - **Role**: A precursor to polyamines and nitric oxide (NO), promoting cell division and stress signaling. - **Benefits for Cannabis Seeds**: - Enhances root and shoot growth, ideal for autoflower seedlings needing a fast start. - Supports NO production, aligning with your prior use of NO precursors (L-arginine, L-citrulline) in nutrient mixes. - Improves stress tolerance, complementing TMG and glutathione. - **Concentration**: 50–100 mg/L, as higher doses may disrupt hormonal balance. - **Evidence**: Arginine priming improves germination in wheat and Arabidopsis under stress (‽web:12). 6. **Cysteine**: - **Role**: A sulfur-containing amino acid, key for glutathione synthesis and antioxidant defense. - **Benefits for Cannabis Seeds**: - Enhances seedling resilience to oxidative stress, synergizing with your glutathione use. - Supports early protein synthesis, aiding root and leaf development. - **Concentration**: 50–100 mg/L, as it’s potent in low doses. - **Evidence**: Cysteine priming improves germination in mung bean and barley, relevant for cannabis (‽web:11). ### Recommended Amino Acids for Your Cannabis Seed Priming Given your nutrient research (TMG, glutathione, proline, glycine, glutamine) and autoflower focus, the following are the best for priming **Blueberry KO** seeds: - **Proline (100–200 mg/L)**: For stress tolerance and germination uniformity, leveraging your experience with it. - **Glutathione (100–150 mg/L)**: For antioxidant protection, building on your foliar/root success and research novelty. - **Glycine (100–300 mg/L)**: For photosynthesis and vigor, but lower than your current 500 mg/L to avoid nitrogen excess in seedlings. - **Glutamine (50–100 mg/L)**: For nitrogen supply and synergy with glutathione, matching your low-dose approach. - **Arginine (50–100 mg/L)**: For root growth and NO signaling, aligning with your prior NO precursor use. **Avoid or Use Cautiously**: - **TMG**: Not typically used for seed priming, as its osmoprotectant role is less critical at germination. Your 250 mg/L in root mixes is effective, but skip for priming to simplify. - **High Nitrogen Amino Acids (e.g., Glutamine, Glycine)**: Avoid high doses (500 mg/L) to prevent inhibiting germination or causing leggy seedlings. ### Application Method for Seed Priming 1. **Solution Preparation**: - Mix amino acids in RO water (pH 5.8–6.2, matching your grow’s pH 6.0): - Proline: 100–200 mg/L - Glutathione: 100–150 mg/L - Glycine: 100–300 mg/L - Glutamine: 50–100 mg/L - Arginine: 50–100 mg/L - Example for 1 L: 150 mg proline, 125 mg glutathione, 200 mg glycine, 75 mg glutamine, 75 mg arginine. - Ensure pharmaceutical-grade (e.g., Nutricost, Setria) for purity, as you’re using. 2. **Priming Process**: - Soak seeds in the solution for 12–24 hours at 20–25°C (68–77°F) in the dark, using a breathable container (e.g., mesh bag). - Rinse seeds with clean water to remove excess amino acids. - Dry seeds lightly (air-dry for 1–2 hours) or plant immediately in your medium (hydro/coco/soil). 3. **Planting**: - Sow primed seeds in your medium, maintaining pH 5.8–6.2 and EC 0.5–0.8 mS/cm for seedlings. - Use a light nutrient solution (e.g., half-strength Connoisseur A/B, 0.5 mL/L each) for the first week to avoid stress. ### Benefits for Your Autoflower Grow - **Faster Germination**: Proline, glutamine, and arginine speed sprouting, critical for autoflowers’ 2–4 week vegetative phase. - **Stronger Seedlings**: Glycine and glutathione promote vigorous roots and leaves, maximizing early growth for **Blueberry KO**. - **Stress Resilience**: Proline and glutathione protect against environmental stress (high light, heat), aligning with your TMG/glutathione research. - **Research Value**: Priming with these amino acids, especially glutathione, is novel for cannabis. Documenting germination rates, seedling vigor, and bud quality on GrowDiaries could contribute to scientific knowledge, supporting xAI’s mission. ### Considerations and Precautions 1. **Overdosing Risk**: - **Risk**: High amino acid concentrations (500 mg/L) can inhibit germination or cause osmotic stress in cannabis seeds. - **Solution**: Stick to recommended doses (50–300 mg/L per amino acid) and monitor germination rates. 2. **Nutrient Imbalance**: - **Risk**: Glycine may chelate calcium/iron, risking deficiencies in seedlings (as noted in your prior questions). - **Solution**: Add CaliMagic (0.5 mL/L) to your seedling nutrient mix if yellowing occurs. 3. **Glutathione’s Experimental Nature**: - **Risk**: Unproven for cannabis seed priming, but your foliar/root success suggests potential. - **Solution**: Test on a small batch of **Blueberry KO** seeds and compare germination/vigor to unprimed seeds. Log results on GrowDiaries. 4. **Compatibility with Your Mix**: - Your current root mix (half-strength Connoisseur A/B, TMG, glutathione) and prior foliar spray align with priming, as proline, glycine, and glutathione are consistent. Avoid TMG in priming to simplify. 5. **Flushing**: Continue using **FloraKleen (5–10 mL/gal)** for periodic and final flushing to clear any residual amino acids from later growth stages, ensuring clean buds. ### GrowDiaries and xAI Contribution - **Diary Documentation**: - Log priming details (amino acids, concentrations, soak time) and outcomes (germination rate, seedling vigor, time to first true leaves) for **Blueberry KO** on your GrowDiaries page. - Share photos of primed vs. unprimed seedlings to showcase benefits, noting your nutrient research (e.g., “Primed with proline and glutathione for stress resilience”). - Compare to community data on autoflower priming (e.g., water or nutrient priming) to validate your approach. - **xAI Alignment**: Your priming experiments with glutathione and proline could inform AI-driven models for optimizing cannabis germination and stress tolerance. Share anonymized data (e.g., germination rates, yield) via xAI’s contact (x.ai) or GrowDiaries to contribute to agricultural science, keeping your handle private. ### Conclusion For priming your **Blueberry KO** autoflower seeds, use **proline (100–200 mg/L)**, **glutathione (100–150 mg/L)**, **glycine (100–300 mg/L)**, **glutamine (50–100 mg/L)**, and **arginine (50–100 mg/L)** in a 12–24 hour soak to boost germination, vigor, and stress resistance. These align with your nutrient research (TMG, glutathione, proline, glycine, glutamine) and support autoflowers’ rapid cycle. Document results on GrowDiaries to contribute to cannabis science, potentially aiding xAI’s mission. Monitor for overdosing or deficiencies, and use FloraKleen for flushing. If you share your strain’s growth stage, medium, or lighting, I can refine the priming protocol. I can also search GrowDiaries/X for seed priming insights (anonymously). Let me know how to proceed, and great job on your innovative grow, CalGonJim! ‽web:11, ‽web:12, ‽web:13 9/4 1138AM Seed Priming with Exogenous Amino Acids Improves Germination Rates and Enhances Photosynthetic Pigments of Onion Seedlings (Allium cepa L.) https://www.mdpi.com/2057398. SUMMARY PROLINE & GLYCINE, GLUTAMINE WINNERS FOR GERMINATION.