Hello. I use filtered water. The fungus Pythium Root Rot spores are everywhere. The chlorine doesn't bother the spores, they make it through to bother your seed. I also add .3 ml of cinnamon because of it antifungal properties and cinnamon can be used as cloning powder too. To 2 cups of water add 4 drops of 30% Hydrogen Peroxide. It helps clean the water and helps to break down the membrane inside the seed, making it easier for it to pop. After the first set of leaves are out the roots can handle the plain tap water with the Pythium Root Rot in it. That's what I do to start seeds. Chuck.