Going Strong!

Hey guys :-) . Today the plant was sprayed again with neem oil. There weren't any more trips to be seen after the second application, but to be on the safe side, you did it again today get a cure. That's why the beautiful dark shimmer on the leaves :-). The ladies are developing well and have 1 week before I change the time to flowering. Normally I would let it get bigger and train because the tent only has 80x80 and 180 watts are available, so I will switch it over soon enough. This week it was poured twice with 0.8 l each time (for nutrients, see table above). Otherwise everything was cleaned and, as always, everything was checked. Have fun with the update and stay healthy 🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://gardenofgreenseedbank.com/candyland/ Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.4 MadeInGermany

Peyote Cookies coming on well. Haven't topped her as I'd like to see her natural structure before making adjustments for the second run. End of week 2 Flower, all Grow nutrients stopped and will just be flowering nutrients till around week 6 flower.

Even though the plant has started flowering, it can still grow a bit during the first few weeks. Mine grew by 10 cm, making it 52 cm in total. I’m adding fertilizer, and the plants look good so far. I’ve been trimming most of the smaller buds that are lower down, but given its size, I’ll leave it as it is for now and see how it turns out in the end.

@Patricia_Zamnesia 💪🏽💪🏽💪🏽

~ GG4 SHERBET FAST FLOWER by FastBuds ~ Well fam, here we go again with another epic strain from FastBuds Fast Flowering stable. After having such tremendous success growing their Gorilla Cookies Fast Flower outdoors last year, I've decided to run another of their fast flowering strains outdoors this year... GG4 Sherbet Fast Flower! The best description of this awesome cultivar comes directly from my friends at FastBuds which is as follows: "Bred from extremely potent and flavorful Gorilla Glue and Orange Sherbet genetics, GG4 Sherbet FF (Fast-Flowering) takes all the best traits to the next level, offering a high-yielding strain that can produce up to 600 g/m2 in a 7-week flowering time. This super resilient Indica-leaning hybrid thrives indoors and outdoors, and in all types of climates while producing mouth-watering sweet, fruity, spicy and earthy terps that translate into a delicious sugary hazelnut aroma. Expect an extremely relaxing and overall happy effect that’ll leave you with a huge smile from ear to ear. It’s the perfect strain for growers of all levels of experience seeking low-maintenance yet highly productive photoperiod varieties that deliver quality and quantity without extra effort. GG4 Sherbet FF grows chunky buds with long dark orange hairs and spade-shaped calyxes that get encrusted with trichomes by harvest time, giving them a gorgeous silvery-white appearance. This medium-sized photoperiod can reach up to 200 cm in height and yields up to 650 g/m2 while developing that typical hybrid structure. GG4 Sherbet FF grows with a stocky, bushy appearance, developing one sturdy main cola and fat side branches that support huge yields without much effort. This super-fast variety produces distinctive light-green buds with a high bud-to-leaf ratio, making your trimming sessions a breeze. It’s a top-notch resin producer that doesn’t need much maintenance and will thrive in almost every climate, rewarding growers of all levels with extremely flavorful resin that makes for outstanding hash end extracts." ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ The Setup: This is going to be an outdoor grow, but I have started the GG4 Sherbet Fast Flower indoors as our weather is still too cold to put her outside (nighttime temp's dipping regularly into the 30's℉). The plan is simple... let her grow inside under a 19/5 light schedule until the nighttime temperatures stay above the mid 40's℉, at which point she'll be moved outside and transplanted into the soil which I have already setup and inoculated with beneficial microbes, and then let the fun begin!🤪💚 ________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Weekly Updates: 5/9- Week Six for the FastBuds GG4 Sherbet Fast Flower and she is doing great! Our weather, so far, has been rather cool and damp. We've had showers on and off every 2-3 days which has taken care of my watering chores which I won't complain about. 5/11- I'm continuing to monitor the leaf damage and am keeping up with the Neem Oil applications to mitigate as much further damage as possible. I still have yet to put eyes on the culprits responsible for the damage to the leaves. Tomorrow I'm going to top dress the GG4 Sherbet for the first time since transplanting outside into her pre-amended soil mix. 5/13- Yesterday, I top dressed the GG4 Sherbet FF with 2 cups of Gaia Green 4-4-4 All Purpose and 1/2g of worm castings. After top dressing and working the amendments into the soil, I watered them in via garden hose with well water. 5/15- There's six weeks of veg for the FastBuds GG4 Sherbet Fast Flower and, with our weather finally cooperating, she's beginning to really take off! I can't wait to see what she'll do over the course of the next few weeks... should be exciting! Thank you for checking out my diary.

good friends ... Good evening everyone! I was really shocked by the rarity of these sprouts! hard as diamonds and cold as ice! we will proceed with the usual drying in the dark with dry trim

2024 August 24: The first days of flowering. She is taller than me and looking really nice, can't wait for the buds!

Video is of Mystery Cookies #1 and #2, with one of their mates, Captains Cake 04/10/2018 Getting ready for a seed run hope to get 1500,or so for a pheno hunt.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Topped and installed the scrog net this week, they took a bit to recover from topping and also defoliated 48 hours after. Changed out the nutrient solution and upped the nutes. I was hoping for more growth at this point but overall I think I'm only about a week behind. Being my first grow, I didnt know what to expect.

Jolie petit buisson 1,08 il resteras petit et touffu il débute les pistils blancs seulement !

last week

​🌿 Journal de Culture : Cherry Cola (Auto) ​Date : 25/03/2026 Âge : 62 jours (Semaine 9) Phase : Maturation (Gonflement et sénescence précoce) ​📊 Paramètres de mon espace ​Température : 24,5°C (Jour) / 20°C (Nuit). ​Humidité : 50%. ​Éclairage : Pure LED 240W réglée à 75% d'intensité, maintenue à 75 cm de distance. ​💧 Nutrition & Arrosage ​J'ai ajusté le dosage pour accompagner la fin de floraison : ​Apport : 4 ml de PK 5-8 (BioTabs). ​Le phosphore et le potassium sont essentiels maintenant pour durcir les têtes et maximiser le poids final. ​📏 Suivi des Plantes & Évolution ​Plante #1 (Celle aux pistils orangés) ​Taille : 41 cm (+1 cm depuis le 18/03). ​État : Elle est la plus avancée dans son cycle. Presque tous ses pistils sont devenus orangés/bruns, ce qui est un signe clair que la plante approche de la fin de sa production de nouvelles calices. Ses feuilles commencent à montrer des signes de sénescence (jaunissement naturel), ce qui signifie qu'elle puise ses dernières réserves pour finir les fleurs. ​Plante #2 (La Petite) ​Taille : 33 cm (+3 cm depuis le 18/03). ​État : Bien que plus compacte, elle est extrêmement dense. Ses pistils sont encore majoritairement blancs et longs, ce qui indique qu'elle a encore un peu de temps pour gonfler par rapport à sa voisine. ​🔍 Mes observations ​Le filet de SCROG est maintenant totalement rempli. La production de résine est impressionnante, les têtes sont littéralement givrées et la couche de trichomes descend jusque sur les grandes feuilles. ​L'odeur sucrée de bonbon devient plus complexe et lourde. La Plante #1 semble prête à entamer sa phase de rinçage très bientôt, tandis que la Plante #2 profite encore pleinement du PK pour épaissir sa structure. On sent que la récolte approche à grands pas .

Semana 3 de crecimiento y parece que todo va un poco mejor. Las plantas tenían demasiada luz porque los leds estaban a muy poca distancia,. Creo que una de las plantas se llegó a quemar,¿Alguien lo puede confirmar? es la primera vez que lo veo. Aunque parece que poco a poco se va recuperando. Desde que las trasplanté sólo he regado un par de veces. Ahora he aumentado la distancia de la luz y espero que crezcan más fuertes. A partir de ahora empezaré con Top Veg. Por cierto! La AK que tenía el caparazón de la semilla pegado, ahí sigue. Y creo que es la que mejor va! Saludos y buenos humos!