Happy 1 Month To My Girls (Inserts Celebration Emojis) I’m Very Proud Of The 1st Month’s Progress These Girls Has Really Exceeded My Expectations. I’m Very Proud Of The Of The Purple Power If You Go Back To Week 1 You Will See That She Started Off The Slowest But She Is Now The Tallest In The Pack Standing At 21 Inches (that’s without adding the few cm I covered with soil) She Has Also Shown Signs Of Purple In Her Bud Development (Favourite Color) Can’t Wait To See Them, You Go Girl! I’m Also Proud Of The Gorilla Glu (12 inches) Stardawg (17 inches) And Girls Scout Cookies (16 inches) Development, They Are My Favorite Buds Here In The UK. They Are Very Bushy & Branchy And Their Stems Are Very Thick. They Have The 3 Main Pillars To Accommodate BIG Buds. Happy Growing Everyone. #OwlGang #AGrowingEmpire

Préfloraison pour la lime bomb de chez aficionados French connection planter tardivement seed test ! J’adore

D57 nearly 2 month since germination also my first autos which are nearly growing at 12-12 only since the beginning. Quite happy so far if i consider they share one pot and have not so much light. D60 It’s going alright 👍 Next to it the buds are developing pretty well. I love it D63 End of the week for those little ladies

Day 32 flower update Emerald Harvest nutes everything looks great...they're fattening up this week...they're all 60-70 day flower times as recommended by the breeders

Not a lot to update the seedlings have came up out the soil at this point they are still to small to tell any visible differences. I started all the seeds from all 10 packs and and I put 26 of them in soil I now have about 20 of them left I still have atleast one left if every strain. I just kept the 20 plants that all started off at the same time it’s so much easier when there is not some plants behind from the rip. In a few days I will pick out the best one from each of the 10 strains and toss the rest so sad there is a plant count here.

Минус одна растиха, и сразу в боксе как-то пусто 😏 Цикл для медицины продолжается, поэтому окончательные выводы будем делать позже. Хотя уже сейчас очевидно, что между харвестами разных сортов будет не меньше месяца. upd 08.11. добавил фото высушенных шишек, получилось даже больше, чем я планировал Спасибо, что заглянули, и будьте здоровы! 🙏

week one of flower looks cool so far no problems with the feeding just used CAL MAG and Mammoth p in the watering the media is holding the water for at least 4 days. going to use the auto flower as a gage for the flowering bump if it needs one.

Finally the cold front has arrived and temps dropped considerably into comfortable levels 22-26 which are perfect for flowering. You can have high temps if you have enough fans blowing at the plant buzt the increčased perspiration can kill your plants through a nutrient burn as they have to take up more water from the res.

Redy to harwest????????????? 😅

June 7 - We ordered some new lights last week, they are 240w kingbrite samsung lm310h with uv/ir, 3000k, and meanwell drivers. We setup the new room and moved the girls into there. After a bit of LST and a watering (with nutrients) at roughly 7ph, they were ready to go under the 2 new lights and the same SF-1000 we have been using in this grow. The new room is a 12 ft enclosed trailer. I moved everything from the small tent into this. I put clear poly on the walls, floor and ceiling. I put poly on the shelf I am going to be keeping in there as well. I bought 50ft of 6mm mylar and lined the floors, roof and sides with it. I plan to get some reflective tape to seal everything and to cover the wood. I did not get much of a chance to watch temp's today as they it was later in the evening after all was said and done. The inline fan blowing in air from outside. June 8 - 9 AM i checked the plants, they were at 25.2 and 50% RH. Late through the day I had checked and we had gotten up to around 32 degrees. So I moved the inline fan to the closer vent, hooked up to that, and had the air blowing out of the trailer with the inline fan sucking it from above the lights. I then added oscillating fan and had it blowing air on the left side of the room so it would somewhat circulate once I closed the doors. I then checked at about 9pm and we were at 22 degrees. Definitely noticed a decent amount of growth already. I have the lights on a 22/2 cycle as I was worried about the heat at night time. June 9 - Some great growth from the girls, still having problems controlling the temps in the afternoon. I decided to prop the door open a bit to have a constant breeze throughout the day while I am at work. Decided to do some more LST and also a bit of defoliation. I took about 25% of the leaf's that were blocking the new growth as it was getting a bit bunched up. I was then told the leaf's are almost solar panels for them. So from now on I will be trying to just do some tucking unless needed. The leaf's I removed were most of the damaged leaf's, I am not to sure if that makes much of a difference. I gave them a watering with only water as there is a potential I am getting a bit of a nutrient buildup along with the PH problem. Or potentially the reason for the ph problem is nutrient buildup. After watering with A PH of 7 I got some run off and tested it. The smaller plant gave me a PH of about 5.5 where the bigger one is around 5.0. June 10 - Plants are looking happy and showing tons of growth. Seems to be trying to stretch outwards. Not a ton of sign of PH issues showing so potentially getting it under control. Still a bit of damage to previous leaf's but it is what it is! The last few days I have been leaving the door open a bit in order to keep the temps down. I decided to test something and turn the lights off (automatically) at 11AM and back on at 5PM so light schedule has now changed to 18/6 and it seems I may have figured out the issue. We haven't had lots of sun the last couple days so it hasn't been to hard and I have yet to know if it truly fixed the heat issue for now. (I will be looking into a ac unit as well since it typically gets to around 30-35 around here. June 11 - Pulled some of the branches back down and added a few more LST spots. Seems we have a good amount of growth from the smaller plant out of the 2 topped spots. Unfortunately it looks like I fucked up on the bigger plant and only one of the nodes seems to have new growth. I will continue to monitor that but I think I cut the node to low and also to soon. Other then that, the girls are doing great. They seem to be absolutely loving these new lights. I gave them some nutrient water today as well. roughly 3L each. They seem to be A hell of A lot more thirsty under these new lights. June 12 - The girls are doing great , they are still just doing growing away. Lots of progress everyday. I am going to be getting a go-pro so I can set-up a time-lapse for the rest of this grow. I received my new inline fan, I got A ac infinity CLOUDLINE T4 with the temperature humidity controller. I am going to be having one fan pushing fresh air in and one fan pulling out the hot air. I will be doing that tomorrow since I have to work today. June 13 - I ordered another 50ft of mylar and that showed up today. I have decided to remove the shelf and add another 3+ feet to the grow space. So today I installed more poly, mylar, the ac infinity fan. I have it set-up to pump in air if it gets to warm. I am thinking of switching it to the output that way if it gets to hot or to humid I can have that air pulled out of the trailer. Right now my other inline fan is the outtake and I just have it set on full. Overall I think everything is set-up a bit better and more accessible. I will be putting my 2x2 tent in there at the left of the doors so I can have a veg room when these 2 are in flower. I plan to have 4 in veg and 4 in flower for the next grow. Still waiting on JOTI seeds, ordered 3 weeks ago and still have not been shipped. I will be getting those going the moment they arrive. The girls seem to be happy still, did a bit more LST to try to keep everything even, mainly I'm just pulling down on the spots I already have tie wire on.

Всем привет 96 день жизни и ровно 5 недель как перевел на цветение. Все по плану. Полил ( 960ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; B-52 - 2мл/л; Влажность 28% - 38%; температура 20c - 27c 15 - й день цвета, Полил ( 896ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; Big Bud Coco - 2мл/л; Влажность 23% - 33%; температура 21c - 29c 16 - й день цвета, Полил ( 1113ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; B-52 - 2мл/л; General Hydroponics CALiMAGic - 1мл/л; Влажность 23% - 32%; температура 21c - 29c 17 - й день цвета, Полил ( 866ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; Big Bud Coco - 2мл/л; Bud Candy - 2мл/л; Влажность 27% - 36%; температура 21c - 29c 18 - й день цвета, Полил ( 737ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; Nirvana - 2мл/л; Влажность 27% - 36%; температура 22c - 29c 19 - й день цвета, Полил ( 700ppm )-3л Sensi Bloom Coco A - 2мл/л; Sensi Bloom Coco B - 2мл/л; General Hydroponics Kool Bloom -2мл/л; B-52 - 2мл/л; Влажность 26% - 35%; температура 22c - 29c 20 - й день цвета, Полил ( 678ppm )-3л General Hydroponics Kool Bloom -2мл/л; Big Bud Coco - 2мл/л; Cal-mag Xtra - 2мл/л; Sensizym -2мл/л; Влажность 26% - 34%; температура 21c - 28c 21 - й день цвета

- Day 63 of Flower Light reduction to 50% - Day 65 of Flower Last time Watering - Day 67 of Flower Lights out for 3 days - day 70 of Flower Harvest Trichomes are about 15% amber and 80% cloudy with 5% clear Temperatures for drying gonna be 19°C and RH around 55%. She is looking beautiful and her Smell is so intensive you cannot unsmell it. Its Loud! Has Some Fruity (Plum or Tangie like), Soapy and Floral Notes to it. I cant wait for the smoke.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Week 3: 2 are thriving 10" and 7", 1 slightly smaller 3", 1 quite small but healthy green and 1 sickly. light is now at full so I will wait while they grow into the light but may lower it depending on how they take the light. I will slowly bring it to 3'. Started the calmag and increasing nutes.

This plant was an absolute dream to. Grow, no issues at all with health of plant, just struggled to tame her as she was a a thick strong climber 💪💪. I'm so impressed with how this turned out, I could have flushed for another 3 days to make it 12days but I needed the space. After The 2 days of dark I was recommended I can definitely say it has vastly boosted her trichomes and the smell when I opened the tent blew my head away 😍😋 Aiming for a 10-14 day hang dry, The 10-12 weeks curing in jars with humidity packs. I will update with a dry bud weight and smoke test 👌 Bud wet weight is just 500g+ Final bud dry weight is 298g. Of juicy resinous purple and orange buds 😍 smells like sherbert and hang dry smoke test revealed a candy sherbert, no banana yet but hoping like the grow it matures with cure process now. Hits eyes straight away, sandy bottom kids, giggles, munchies, fun time with Mrs, bed. Full cycle with this one very happy over here 😉😁💚 FAT BANANA AUTO FROM ROYAL QUEEN SEEDS, big thank you to the team, and the breaders. What a pheno I received. I appreciate you all

E arrivata alla fine. È ora di taglia

Here we are at the harvest of one of the masterpieces of the season, Ghost Train Haze Automatic by Zamnesia. ** We remind everyone that we have grown two plants per strain, one without the aid of any technique and the other instead worked with the techniques described below. Technique used // The plant was worked with the MAINLINING technique, particularly successful in this case because the plant with a wide internodal distance put a lot of body until it swelled like a pig. Better with the techniques or normal? It is a plant with a high distance between internodes, the Mainling was truly perfect. This time I am strong for the main lined plant. You can watch plant and I believe it will think the same This is another confirmation that MAINLINING on AUTOFLOWERING in my opinion is only indicated in plants with medium-high internodal distance. This is a real monster not usual for soil in 11 lt pot. Flower quality // The flowers are impressive, the classic haze shape is very long, some are really hard and heavy, I'm not crazy about the taste but by definition the pure haze taste is not among my super favorites but I love it anyway. Check the article in your language // https://www.zamnesia.io/it/guida-growing-cannabis/333-lavaggio-delle-ceme Trichomes // Here we have an absolute majority of milky and cloudy trichomes, still some transparent and some amber, it is not super ripe but for me it is fine. Fertilizers // I am very happy with how I managed to unload the plant which at a certain point before going to flush was fed for a few days only with additives and then went to flush and we are discolored enough. This is a yellow that satisfies me to go to crop and vaporize a delicious plant with no residue of nutrients inside. All Plagron fertilizers and additives in the green / 100% organic version have fully satisfied me. Choose your soil according to your style and calculate the sheet on the website www.plagron.com The fertilizers instead are found in convenient all-inclusive packages on the Zamnesia website. Try a seed of this variety belive in Zammi i superlove this strain.. ---- https://www.zamnesia.io/en/12511-zamnesia-seeds-ghost-train-haze-auto.html Zamnesia Description // From the day her cotyledons emerge from the substrate, Ghost Train Haze Auto will surprise you with her rapid growth and robustness. When she starts to flower, this short sativa will develop long and compact buds with pungent scents of spice, citrus and fuel. Music and frequencies // For about 4/6 hours a day my plants listen to 432 Hz frequencies made especially for their well-being and sometimes a bit of black music to give them those vibrations that only music can give. www.radionula.com All the best that mother nature has to offer is on ---- www.zamnesia.com THE FLOWERS ARE MONSTROUSLY LARGE. HOLY MARY.

Flowering day 37 since time change to 12/12 h. Hey guys :-) The buds have developed incredibly nicely this week. The smell is getting more and more extreme :-) . The whole plant itself looks perfect 👍. The growth has slowly stopped as the full energy goes into the production of the buds. This week it was watered 3 times with 1.2 l each (nutrients see table above) They were individually inspected and checked for their health. Osmosis water for the coming week was refilled in a 100 l tank 👍. Have fun reading the diary and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.barneysfarm.com/dr-grinspoon-5 Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Drying time