Day 63/64 CPK. Starting week 10 just watered pH in 6.35 pH runoff 6.5, defoliated also. Day 66 CPK. Fed nutes today. It's getting hard to control the pH probably salt retention. The pH in was 5.9 and the runoff pH was 6.7 so she is ready for a little flush on the next watering Day 69 CPK. Water only today, mini flush to a half gallon runoff. End week 10. 3-9-22

Day 11. Week 2. Hello. I'm a little late with the report. I see growth, but slow. I think the soil was of poor quality. I added fertilizer to the water, after a day I saw improvements. You may need to water less. On the second video on April 15th. Share your experience. I will be glad.

Update: Day 1 of week 11 - Thursday 29th October I am soooooo fucking pissed! I don’t know whether watering the girls from the bottom has made the girls more thirsty or just that they are generally more thirsty due to needing more Nutes during flower but one of the ladies has wilted quite terribly. The other girls seem ok, slightly more thirsty and the leaves slightly droop a little more than usual at feeding time but one girl has defo been through the thirst wars.. She’s bounced back after a good water but the buds looks shrivelled and feeling very sorry for them selves. I’m not sure she will be as good a smoke now if she makes it at all.. Other than that moved the LED up slightly more to keep a good distance between the light and the girls.. I really haven’t got anymore space now so fingers crossed this all works! Super vex this has happened in the final weeks. UPDATE: Day 2 of week 11 - Friday 30th Oct The wilted lady has made a great recovery but the buds still seem a little dishevelled. But considering what’s happened to her I’m happy so far.. Let’s see how she goes🤞🏼 UPDATE: Day 3 of week 11 - Saturday 31st Oct All looking good and buds seem to be fattening up a little now ❤️ Nutes in as normal and happy the wilted lady has made a good recovery.. UPDATE: Day 5 of week 11 - Monday 2nd Nov Nutes in and all looking good.. They don’t seem to be stretching much now and I’m hoping that means the buds will fatten up nicely from here on in.. UPDATE: Day 7 of week 11 - Wednesday 4th Nov Nutes in and another week done.. Come on ladies it’s time to fatten up for me please 🤞🏼🙏🏼 Happy Halloween everyone 🎃 👻

Day 30 Wed PH 5.7 EC 0.6 DLI 18h PPFD Water 24c Day 31 Thu Add Ph Up PH 5.3 - 5.9 EC 0.6 DLI 18h PPFD Water 21 Day 32 Fri Add water Ph Up PH 5.5 - 5.7 EC 0.6. - 0.6 DLI 18h PPFD Water 22c

girl, from the beginning she was watered with water from the tap, the value of which is almost 350ppm. and a lot of lime, which prevents me from growing it bigger, but I'm happy with the result :) it's a small pot and only 50 w :) the flowers look very beautiful and sticky :) super ​

D85/F41 - 24/06/23 - She's almost ready, I think I'll start the flush soon D86/F42 - 25/06/23 - Temp is still too high, I'm trying to refresh the environment with air conditioning D87/F43 - 26/06/23 - First Thricomes Video. I'm going to start the flush today and I'll arwest next WE D88/F44 - 27/06/23 - Flushing D89/F45 - 28/06/23 - Flushing D90/F46 - 29/06/23 - Flushing D91/F47 - 30/06/23 - She's ready. Tomorrow I'll cut her

Last leg of the race for some of these girls! Excited to harvest and trim and cure them. Everything went fairly smoothly this week, looks like some serious burn on the one, but she is towards the end anyways. I started flushing some of them a week ago, and the one furthest along about 2 weeks ago. I’m glad because I’ll save on nutrients as a bonus! I’m excited for my next run, I think things have gone really well with this first grow despite all of the complications. Anyways I should have some harvest pictures next week, thanks for following my grow!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Deep Forrest auto is growing great. She is producing more pistils, and starting to stretch. I have been switching her feed to the blooming nutrition. She has been growing really strong. Everything is ready to go for another week. Thank you Doctor's Choice, Gen1:11, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Harvest day, I want to thank you for joining me until the last day of cultivation

💫The week went great. After the first stress, growth seemed to slow down, but it worked out, the plant began to expand. I water it every 3 days. I continue to bend the stem along the axis of the pot. I bend the branch that will pass through the fastest. 💚I started giving supplements late. Only on the 33rd day I gave him fish and everything else. 🍀 Week showed good growth, but it could have been better, let's see what happens with the additives. I continue to twist the stem in a circle (I don't know where to twist it). And I also cut off the leaves a little bit, about once a week, as they block the light (a friend says not to overdo it with defoliation, let's see what happens).🍀

This week i‘m forcing her to bloom. More nutrients and more ‚Sanlight‘ haha. Training with the clippers didn‘t really impress her, so she’ll be fine with the new setting. :)

I think I ran into a slight nitrogen toxicity as the leaves are dark green and have a slight tip burn. I think I will change my calmag to use one that has lower nitrogen. Still looking good

This is the continuation of Everyone in the pool. I got locked out of that Diary. So we will continue here. I will add everything slowly, but we will move on. Things have changed.... surprised, I'm not. It's time to move forward for summer. So I don't really know how this will work. But it will work out. So everything looks super happy. Even Groot... *Citrus Farmer girls are going crazy. One has the stalk of a weight lifter. Its huge literally huge. Her sister has some beautiful branching. *Sour Fire Kush is also going crazy. Another body builder also. Her branches could support a house. *Strawberry Lemonade is flowering nicely... she is kind of hiding from me. *Fire OG clones have been moved to flower. *Tahoe Kush is still adjusting to being transplanted and moved to flower. 3 more days and they will start to grow. Sometimes it takes about a week to adjust. They were root bound. I am going to be pissed later that I didn't take clones. *Slurricane mother really needs some trimming, or I just need to chop the shit out of her. She is huge. But I need to keep her and my Fire OG mom on the back burner. They are on the life long veg. So I will be adding my summer trees. I think.... Fuck it everyone is going to be here. *Dominion Skunk is up. Need a girl to Love. I sprouted 4 so I pray we have a beautiful girl. And shhh I'm going to put *Blue Monkey down tonight. I will try to sprout 2 of these Girls. They are feminized. One will be a tree. But dont tell, it's bad luck to talk about them until they are up. So I will soak for 24 hours, and then into root riots. On a heat mat in a seed starter dome. So let's continue where we left off.

This week, I gave my plants more fertilizer to test if they show any signs of burning. Now, I'm stressed out! I think I should give myself some seaweed to relieve the stress 😂 The girls show signs of calcium deficiency. I sprayed a small amount of calcium and magnesium on them to help them recover faster. I’m not sure why the new leaves are very pale. Could they be lacking iron? I think the high soil moisture might be causing issues with iron absorption, but I’m not completely sure. I’ll wait and see what happens this week. Considering that I use regular water, I expected them not to have a calcium deficiency.

The girls are doing fine this week. They are packing on some weight and I'm starting to get a sweet, coffee smell from some of them. I'm noticing at least three different phenotypes, which was expected with F1 seeds. That said, all but one gave a good stretch, and each of them is showing great bud development.

Well everything will be done come Sunday I did stick another runtz in 48 hours darkness she was begging to go stink my seeet smelling girl she was the others Friday I’ll switch the whole tent off for48 hours and that’s that will be starting another grow up after I clean everything up stay tuned for harvest photos and weights happy growing