This is the first day of week 4. I decided against any form or training or topping and decided to focus on watering and getting nutrients correct for this first grow. Being it’s a tent grow, I can essentially grow when I choose now anyway. Touching the plant leaves the soft but pleasant smell on your fingers that we all know and love. I’ve also drilled more holes in the bottom of the pot and do get some runoff when I water now. Considering switching to 12/12 in a weeks time.

For now I'm happy with the germination,Even the transplant in the organic coco hydro growth modules all right yesterday✌️🏿

Ran out of headroom in the tent, WW#2 is sitting at about ~49 Inches Tall with NL 1&2 close behind at around 42 inches. WW#1 is flowering nicely and sitting at 27 Inches, propper up on buckets and with added supplemental lighting which will also help the lower budsites on main colas on the other plants. Hope that WW2 doesnt stretch any further or i'll have to start training it a little to keep it away from the light but growth is definitely slowing at least.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Couple got a little burn I think maybe a little too much soil in there with the coco on top of the light feed? I got all the final pots filled up today they will go in 3 gal bags either today or tmm. It's a Greentree pro ultra coco/perlite mixed with some roots organic soil about 70/30.

Day 14 🤩 vegi is starting Piccas from this morning 8.30 and 17.00 after LST So today was the first time I had Terra, Generator & Root Stimulator, only ¼ of the specified amount of everything and of course Hy Pro Fertilizers, my fertilizer barrel is finally ready, is being supplied with oxygen and the fertilizer is in motion, which means that as I am lazy I make 50l and leave it in the corner 😉 but always ready to use... Then today was the first light LST, I will leave it alone for 4 days and then see where and how I pull it out, it doesn't really matter because I want to put up a net and lay it flat underneath The Frosted Agave from Original Sensible Seeds has also seen the light of day This grow is sponsored by: Zamnesia Seeds Hy-Pro Fertilizers Mars Hydro LED Start 26.4.024 Germination date 30.4 024 Germination 6v6 From Zamnesia Do Si Dos f1 Auto.2x Green Crack f1 Auto 2x Pineapple Express f1 Auto 2x Growing method L.S.T with the help of a scrog net..🙈 Light led 1x fc 4800 from mars hydro Exhaust air, I replaced the heavy pk 150 with my Mars Hydro 6 inch fan Filter Rhino pro 900m³ Inlet air 305 m³ with filter 160mm Soil: Atami Light Mix & 5 mm pumice stone & 16 mm expanded clay on the floor In 15 l. Factory pot Temperature inside: 26.50°C 79 Fahrenheit Outside temperature 21°C R/H 64% Light: 19/5 dimmed to 75% distance 70cm Roots 21.00°C Ph medium 7.0 Ph water 6.3 1.5 liters today Ec 0.8 Nutes Hy-Pro Fertilizers Terra ml to 1l Terra Veg & Bloom 0.6ml Roots Stimulator 2.0 ml Spraymix 5 ml Generator 0.3 ml Epic Boost 0 ml Enhancer by Greenhouse 0 g 😉🤜🤛 #marshydroreview #HyProFertilizers #zamnesia #originalsensibleseeds

Oh man I'm excited about this one now. I think I may try and fill this tent out with this just this one plant. Earth box and manual scrog on this Gelato. After getting her in there and realizing how easy she is to bend over and how easy it will be to get a monster plant with this set up, I'm super stoked. This will probably end up being a super long veg time but that's okay. This is exciting for me. Something different.

Whats up GD !!! ------------- GROWTH------------- Topped both plants, the bigger one, it is 3 days younger than the other, and seems to have faster development, more vigorous.. I think the reasons could be the pheno or the level of root development, i ll explain it : C#1 was in a yogurth as seen on photos and having all the sun. C#2 was in a wider recipient, lower in height, and in a room with poor light. I watered both plants at the same time, C#2 got curvy leaves when watered, i think maybe she likes near dry soil, or cant handle that level of humidity in the roots ... in my opinion she has less developed roots and the pot hadnt enought holes to let the air come in, the oposite of C#1, that seems to handle better humidity and is eating all she can, getting fluorescent green below sunlight. Both plants have same feeding and soil composition. ------------- IRRIGATION------------- Watered both ladies, around 0.2-0.3l each one with Deeper Underground. ------------- NUTRIENTS------------- Tall cheese C#1 : Deeper underground 1ml/l Short cheese C#2 : Deeper underground 1ml/l + Micro Vita (it only had Canna Pro soil ) --------------------------------------- Good vibes !! _12/04/2018_

This plant came from a random seed I found in a dispensary bag and since we have dozens and dozens of bags I don't know which one it came out of...so the strain is unknown. I’m not sure whether it’s a photoperiod or an autoflower, but I’m assuming it’s a photoperiod for now. I started it indoors in a tent, germinating the seed using the paper towel method before planting it in a small quart-sized pot. It’s been growing for a few weeks in Fox Farm Ocean Forest soil, but the leaves have just started to fade, so I’m preparing to transplant it into its final home—a 50-gallon pot outdoors now that it’s spring. The 50-gallon pot is filled mostly with standard potting soil, mixed with Fox Farm Ocean Forest and perlite. A few weeks after transplanting, I’ll begin feeding it a combination of liquid nutrients including Advanced Nutrients, Fox Farm, CalMag, and Mikrobs Microbial Superpack. I’ll also top-dress with worm castings and other amendments as needed. If the plant turns out to be male, I’ll harvest the pollen or any seeds it produces. If it’s female, I plan to grow it as large as possible—which is why I’ve chosen such a large pot and moved it outdoors.

Week 3 Running light at 40% Viparspectra DWC water system working normally Increased nutrient intake as week 1 Humidity lowered to 40%

2/15 NL: I seem to be in the final stretch of things! Lots of white pistils still, but they're starting to curl in. Going to keep her in the tent and recheck this weekend! Harvest window is approaching! Very excited for the extra tent space. 2/19 NL: Not quite ready for harvest. Soon...

Woche wird noch beschrieben ... in der nächste Woche

amazing growth this week from all 3 plants. The peanut butter cookies is recovering from the transplant. Both the auto cheese and auto cinderella jack have both started to show signs of flowering and have bushed out incredibly well. The pbc has had its first top done and has started growing 2 extra nodes. 07/08/2020 - training has been done on all the autos. They have recovered extremely well and seem to not be slowing down. Finally starting to see the type of flowers I have been striving for. After a semi unsuccessful first grow, this has been a huge relief. Both autos are now showing signs of going into flower so I will try to stop LST unless any unruly branches form. The peanut butter cookies has now started to fully recover from the transplant and topping. Will now allow this to flourish and grow as big as it wants as both autos should be done before its too big (hopefully)

I’m really just updating with photo dumps at this point lol but this white widow is proving to be f*cking massive and these buds are looking like they’re going to STACK!!! I defoliate here and there lightly and open the canopy to get some light further down. She fills a 2x2 out completely! My next run will consist of a few of these for sure!! Should be a great harvest!!

Week 9 for Lemon OG by SSSC Ive pulled her apart to see if she gets some equal growth all the way around her. Still trying to stay positive for her to grow more😂 Shes also going to be moved outdoors today likely into some shaded area as its been so hot outside lately. Lets hope her transition goes well.

Gone in to flower so started on some bloom nutes this week and also gave them a good defoliation

J’ai coupé et mis à sécher 1 des 3 . Après 3 bon rinçage j’attends que le substrat est bien séchée avant de couper les 2 autre.

Waiting for them to stretch after transplanting them