2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

This week, Satori recovered from the stress after HST and is ready to go into flowering mode, but I am waiting for blueberries. The blueberry leaves are all cut off, but the flowers are intact, the vegetation is slow, I hope one more week 18-6 will be enough.

Vamos familia con la décima y última semana de floración de estás Cereal Milk de RoyalQueenSeeds. La temperatura está en 22 y la humedad actualmente está en 45%. La cantidad de agua cada 48h entre riegos no cambia, y por supuesto controlando el Ph entre riegos alrededor de 6.5 - 6.2. Ya se ven bien sanas estas 5 cereal milk, tienen un color y una salud espectacular, y ya están desprendiendo buenos aromas, vemos como han madurado las flores, ya estallaron. Ya llevamos 2 semanas solo con agua, les daré machete ya en que acabe secar la maceta. Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Hasta aquí todo, Buenos humos 💨💨

Week 16 Everybody is on Flower! End of August: daylight 13h20: 7h20/20h40. Day temperature max: 26°C - Night temperature : 17°C Sunny weather - 2/3 rainy days in 2 weeks Enriched the soil with seaweed powder and bat guano. Continue to add flowering nutrients: Grower Master I added some Calmag one time. Watering 2L/plant every 3 days (2 days would be better) With humidity growing and the relatives hot temperatures: the predators are attacking plants. Everything appears so fast: Aphids, caterpillars, leaf miner flies. Did a general defoliation before spray my mixture Garlic + laurel (12cl Garlic juice + 5cl laurel infusion + 1L distilled water: beware PH!!). I sprinkled diatomaceous earth on top of soil to protect and minimize the infestation. Brake Pad Breath: still deep green and bushy. Flowering since 4 weeks. The most advanced, pretty good flowers with long pistils are growing. Spared until now: the plant is victim of Aphids, miner leaf flies and caterpillars in a « reasonable way »….try to eradicate the problem without shocking the plant at this stage. Flash Back#2: around 2 weeks flowering, stretched a few centimeters more, well developed plants with lot of blooming sites. Relatively spared by pests, just some aphids. GMO X Zombie Kush: some difficulties to recover….just engaged the flowering process, not to bad if she resist to the second pest’s wave. Royal Moby: uncertain destiny….recovering good, sativa style: 155cm, just starting the flowering stage…super late! Harvested a pretty auto flower: White Russian by Serious Seeds. Well developed plant, a little bit long to be ready but resinous and meaty buds.

8ème semaine pour ses dames,flush des racines.

6.12.25 6th week flower started on 6.9. Just like my other 2 grows going right now this one got stunted really bad and started putting on flower way before recovery so here we have it another miniature plant, one of the smallest I ever grown maybe even seen! Oh well excited for next grow! Thank you for looking at grow I appreciate it!!!

SUMMARY: Nothing interesting this week...she's receiving 3.5-3.7L of late bloom nutes per day providing roughly 20-30% runoff each feed. She's still skinny and I'm thinking this one is going to be a low yielder all things considered. I do think that I was late introducing bloom nutes and this has hurt my final yield. DAY 79 ----------- Friday 12th March 18:00 I fed Jesus 3.2L of late bloom nutes which produced 620ml runoff (19%). [26°C/48%] DAY 80 ----------- Saturday 13th March 19:00 I fed Jesus 3.5L of late bloom nutes which produced 580ml runoff (16%). [26°C/48%] DAY 81 ----------- Sunday 14th March 11:00 I made 15L of late bloom nutes with rainwater and molasses. 5L at 5.8, 5L at 6.0, and 5L at 6.2. Sunday 14th March 18:30 I fed Jesus 3.7L of late bloom nutes which produced 840ml runoff (23%). [26°C/48%] DAY 82 ----------- Monday 15th March 11:00 I made 5L of late bloom nutes with rainwater and molasses at a pH of 6.3. Monday 15th March 18:00 I fed Jesus 3.7L of late bloom nutes which produced 960ml runoff (26%). [26°C/51%] DAY 83 ----------- Tuesday 16th March 18:00 I fed Jesus 3.5L of late bloom nutes which produced 1L runoff (28%). [26°C/51%] DAY 84 ----------- Wednesday 17th March 18:00 I made 15L of late bloom nutes with molasses and rainwater. 5L at 5.6, 5L at 5.8, and 5L at 6.0. I fed Jesus 3L of late bloom nutes which produced 420ml runoff (14%). [26°C/50%] DAY 85 ----------- Thursday 18th March 18:00 I fed Jesus 3.5L of late bloom nutes which produced 980ml runoff (28%). [26°C/50%]

End of week 5. Plants have grown a lot. Nice stretch last week. Nice bushy plants, make sure to do defoliation .these will take longer than the other FastBuds strains I have going at the same time. Lights and genetics working great

Start of week 6 of flower and the smell is incredible the buds are really starting to fatten up I had to tie a couple side branches to the main stem to keep from snapping can’t wait to see how big they get this week going to let them go for another week or 2 before I start the flush still shooting out white hairs (Update) One of the plants is starting to put out some purple colors same environment and strain different appearance (Update) the two biggest plants are maturing faster then the others trichromes are milky a little amber was going to start flush week 9 but I started these two already If you like leave a like 👍

Giorno 50...🌻..la ragazza va alla grande fatto il cambio di soluzione per la parte finale di questo ciclo... Tutto procede bene non vedo l'ora di raccogliere questi frutti...🌱🌻 Credo che la Critical dovrò raccoglierla prossimamente 💚...comunque controllerò con il microscopio lo stato delle cime e si vedrà...🔝 P.S. rispettando i tempi dati da RQS il raccolto dovrebbe arrivare il 20/05 (9 settimane) Giorno 54 aggiornamento foto 15/05/21 Ieri sera ho controllato lo stato di Tricomi con il microscopio elettronico ancora c'è una buona percentuale trasparente da lunedì controllerò giornalmente per raccogliere la maggior potenza💪🚀... Un abbraccio a tutti i visitatori.... 💚👊

Wow week 8 and Pineapple Express#2 Fem seems to be flipping to flower.Our Elixir CBD Auto is in full flower and continues to give off these peppermint vibes, this is definitely a medical strain and even though shes so small i have learnt alot from being sure to keep your Autos in full sun at all times during the vegetative stage ro ensure maximum yeild.From what ive gathered is that the strain itself isnt a big yeilder and due to the fact that she has not had enough sunlight we will end up with the small head you see here.The Good news is i still have one seed of hers left and next time i will be planting her will be in season😁.Our Hindu Kush Auto continues to flower beautifully and her smell? Nothing short of incredible😍..Our Purple Kush CBD Auto has impressed me most of all, she seems to have reacted so well to the training and the nutes as well as the minimal sunlight conditions.She stands at about 17 cm in length and her side stalks are about 10 cm in length..Our Girlscout Cookies Auto has exploded and is overtaking her grow bag rapidly, hopefully her training and folage removal has worked in allowing her to grow even larger now...Our Blue Cheese and Granddaddy Black are growing up quick and with all our strains having now received some light folage removal i expect they will use that extra energy to really bust out.

Ordered a scale the other day so should have some more numbers tomorrow! Not going to sample anything until I get the scale, then I'll update with some more details.

***DIVINE SEEDS ***** *****OPIUM***** Sponsored grow Week 4 Germination April 6. Week 1 water only Week 2 water only Week 3 added recharge and* TPS1 increasing ppm to 570. Week 4 added calmag, did LST and defoliation. Before & after pics. Increase ppm to 685 This week 4/28-5/4 consisted of plain ph 6.6-6.9 water with recharge, calmag and TPS1. 4/24 updated my ACInfinity app and started using the advanced programming settings for early vegetation. This is keeping humidity in 70% with temps in 80. What I don't like is using advanced programming, the humidifier is at level6 . This spews moisture directly on my plants, into the drain tray and floor. 5/1 I did some LST and defoliation. Before and after pics above. Until the plants are big enough to go outside, I will be using my AC Infinity tent and equipment. Once outside, I will be watering with well water and recharge and any additional fertilizers or amendments will be determined at that time. Thank you @DivineSeeds Thanks for the likes and comments, I appreciate all the plant love💚. Have fun & love what you grow 💚 Sending love and light 💫 💫Natrona 💫 DIVINE SEEDS ***OPIUM*** Harvest:1000 g/m2 Divine Seeds developed Opium as a new champion strain, unprecedently powerful and loaded with unbelievable quantities of resin. Certainly, no average genetic base could be behind such brilliant parameters! Several Afghani Indica landraces, recognized as most potent and resinous, were bred together with a mighty Brazilian Sativa which Opium inherits its bright berry taste from. Then a great deal of work followed aiming for the highest THC level and endurability as well as a branchy structure that would provide maximal yields. Now we can proudly title Opium the best Indica you could dream of growing! Indoors these plants mostly stay mid-height (1.5 m), reaching up to 2.0 m out of doors. Depending on your height limitations, Opium flourishes universally in grow boxes, balconies, green houses and terraces. Due to its Afghani parentage, this strain can stand hot weather if provided enough water and some shadow. Responds well to any training techniques: ScroG and SoG, LST, topping and FIM, supercropping. The start of blooming is marked with a rush of growth, when plants stretch almost 1.4x. Expect heavy colas that often require additional supports. Whilst ripening, Opium gives off a pungent earthy stench with fruity and berry undertones, also a bit of pine. Ready for outdoor harvesting in October. The stoning psychedelic impact of Opium is like being hypnotized (unless you’ve had too much, then it feels more like a blackout). Stone-lovers will appreciate every minute with Opium: its spicy taste immerses your mind into daydreaming, while the body rests flat. Recreation with this psychedelic Indica means silent tripping from one insight to another. Mighty enough to kill chronical pains, Opium is also medically efficient against insomnia, nausea, appetite disorders, muscle spasms, headaches, irritation, fatigue, panic attacks, epilepsy. Best consumed at night time, the effects keep going for up to 4 hours. Equipment and nutrients Pots: 5gallon Air pots Soil Fox Farm Happy Frog Amended with worm castings, dolomite lime and mychorihiza Recharge Seeds provided by Divine Seeds Divine Seeds breeding company The link to Opium Feminized Seeds Opium - Divine Seeds breeding company The link to Opium Auto Seeds Auto Opium - Divine Seeds breeding company ================================= Equipment: AC Infinity CLOUDLAB 844 – Advance Grow Tent 48”x48”x80” CONTROLLER 69 PRO – Grow Tent Controller CLOUDLINE LITE 6 - Inline Fan 6" IONBOARD S44 – LED Grow Light Board 400W CLOUDRAY S6 – Oscillating Circulation Clip Fan Carbon Filter 6” 4

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The two larger (and older) CBD Cracks are looking very good, the younger one needs to kick it's butt into gear! We have a heat wave going through right now so hopefully they'll like it😎 I went out to water early this morning so they got a little lime water. I was hoping for a little more growth this week but hopefully the lime will do it's trick and help them along. They have plenty of nutes in the soil to use up and I don't want to mess with them too much early on. And yes, I did put tomato cages in their bags because my evil, spiteful cat was trying to dig in there and take a 💩 and I really don't need that...