don't worry I'm still here and the grow is going well so far

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Post tropical storm Ophilia.

Still looking smaller than usual but I think it could be down to the genetics I’m not sure, I saw a little brownish/orange burn spot on the tips of Grapey Walter #2, it dosent look like nute burn but not to sure it’s an issue at the moment. Did the first bit of LST of these girls tonight and hopefully they will respond well and start bushing out letting the dident branches catch up.

My first Grow with random cuttings. I found growdiaries after the Haverest.

Huge signs of progress this week! The smell has come in strong as well as the nice fat crystal flowers starting to plump up, Definitely a tall growing strain but all signs are pointing towards the yield being of a good quality! Have ordered a USB loupe that will be arriving tomorrow as the current is too hard for photos!

Not much to report this week so far. Slightly defoliated above the Scrog to allow more light penetration for the lower branches. Plants starting to stretch abit now but nothing to bad.

day 38 stopped giving PK, carbs. only humic, folvic fishsh!t in watering. lifted terps video sticky icky video day 39 just humic and folvic into water. day42 bridal party gained a lot of weight, started to lean over blackberry. I feel like I could chop today.. slight chance might meet remo :)

Day 50: 150ml water per day, 18/6 light schedule, approx 50% humidity, fertilization on Monday, Wednesday and Friday happens with BioBizz family and on Tuesday, Thursday and Saturday with bat guano and Epsom salt. They like the new Marshydro 600W. Their height are different between 15 and 45 cm. Day 55: This tent is crazy :D All of them okay, especially Critical Orange Punch is a monster above all. Sour Stomper is cute, and the rest of them just keep flowering. Hopefully their buds will be nice thick :)

Legend Timestamp: 📅 EC - pH: ⚗️ Temp - Hum: 🌡️ Water: 🌊 Food: 🍗 pH Correction: 💧 Actions: 💼 Thoughts: 🧠 Events: 🚀 Media: 🎬 D: DAY, G: GERMINATION, V: VEGETATIVE, B: BLOOMING, R: RIPENING, D: DRYING, C: CURING ______________ 📅 D64/B27 - 18/06/24 ⚗️ EC: 1.0 pH: 5.2 🌡️ T: 26°C H: 80% 🌊 FLUSH 🍗 💧 💼 FLUSH first day 🧠 🚀 🎬 1 TL video ______________ 📅 D65/B28 - 19/06/24 ⚗️ EC: 0.2 pH: 7 🌡️ T: 26°C H: 80% 🌊 FLUSH 🍗 💧 💼 FLUSH second day 🧠 🚀 🎬 1 TL video ______________ 📅 D66/B29 - 20/06/24 ⚗️ EC: 1 pH: 6.2 🌡️ T: 26°C H: 80% 🌊 15 L 🍗 CalMag - Bloom A-B - Bud Candy - B52 - Overdrive 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D67/B30 - 21/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D68/B31 - 22/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 15 L 🍗 CalMag - Bloom A-B - Bud Candy - B52 - Overdrive 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D69/B32 - 23/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D70/B33 - 24/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video

Y nos fuimos a floración!! 🍀👨‍🌾🏻

1/4 - start of week 5, the broken branch isn't looking good. I installed the 5 arm light which is my biggest light for the veg compartment. 500ppfd right now, drop it 1 inch and I'm just a bit over 600 at canopy level. I'm hoping to get her to recover and ill bend the 2 remaining branches towards each other to fill the gap for a 3 arm lady, still planning to top but now will get 6, 12, and 24 tops...hopefully 1/5 - slight training day, I bound the 3 branches so that they were more uniform and evenly spaced out in the pot. I'll let her take shape a few days with the restraints and the. Top her maybe Friday. 1/7 - she got her topping. I'm leaving 2 nodes on each branch for a total of 12 tops right now. She will get one more topping before its all over for a total of 24 tops, probably 2 weeks out on that. Feeding in the next day or 2. Update: dropped the fan speed to 2 for temp and humidity testing. 1/9 - RAW Feeding tonight, she is starting to recover well from the topping, I now have 15 or so tops with one of the 3 arms splitting into 7 tops rather than 4. I think I will leave her this way to veg out to the height I need without further topping. I’m planning on training the shoots out to the sides so many more tops may develop as new nodes come through. I’m just really hoping she doesn’t start to flower on me early!! 2L distilled water 3g Grow 2g Kelp 0.57g Cal/Mag 2g Microbes Grow Stage 0.63g Amino Acids 0.14g Enzymes 6.0ph 66.9*F 1060ppm Also found a new way to mix my RAW nutrients without having them stick to the bottom...small landscaping pond pump! It gets the water moving nicely and I just drop the dry nutes in, mixes it for me and I can easily fine tune PH and test PPM without having to shake and take samples! Huge win. 1/10 - Another week in the books. Did some fan leaf tucking so the tops had direct light. I'm still trying to grow her sideways so I'm thinking a solid 3 or 4 more weeks of veg. Things should speed up now though. I dont think I will be topping again but we shall see. Topping again would guarantee 30 tops which according to my math is a bit too many for my grow space.

Some browning from start but cleared up in the next week

Aroma is extremely strong, pistols are maturing, bud sites are becoming more dense, anthocyanins doing their job on this pheno, fan leaves began yellowing due to slight heat stress. Approaching Harvest

Kannabia Run – Flowering Day 7 🌸 It’s been 7 days since we flipped to 12/12, and the stretch is already happening! 🌿✨ The plants are growing fast, and we can already see the first pistils showing — a beautiful sign that flowering has officially begun. 🌸💚 Everything looks healthy and strong so far. The Monkey Grease in particular are showing a lot of potential — these ladies clearly want to grow big! 💪🔥 Some good LST will definitely help shape them and maximize their yield. Excited to see how they evolve over the next weeks! 🚀.

Welcome Back!💚 Die zweite Blütewoche ist ins Land gezogen und die Pistilen an den Blütenansätzen entwickeln sich langsam aber ständig Der Stretch hat nochmal knappe 8 cm an Wuchshöhe mitgebracht. Die Blattentwicklung war nochmal sehr rasant, so dass ich die Sonnensegel nochmal stark ausgedünnt habe. Die Werte im Zelt sind optimal auf die Blüte eingestellt. ——————— 🌞 Temp: 24°C 🌚 Temp: 20 °C 💨 RH: 56% VPD: 0,97 kPa 😎PPFD: 830 mqm ——————— Stay Tuned! 💚

This was the last week of veg. I will switch to bloom nutrients from advanced nutrients. So far so good. I lowered the ec by 0.3 a few days, because I got a slight tip burn. In my previous grow I had to deal with nutrient lockout, I am trying to avoid that this time. I measure the values of the water tank 2 times a day, In particular the ph values keep rising daily. I will defoliate when they are done stretching , around week 2 or 3

Ok so I'm a couple of days later with posting, though the images are of last Friday. They went full into flowering. At the same time, 3 of the 4 have some weird growth going on. Deeply ridged leaves, a bit springy plants. I raised the lights cause I also saw white points and other leaf burn. I have to get used to the nitro-fueled energy of these thoroughbreds and the 'on the edge' style of growing that autos deliver, as a mostly photoperiod grower, this is both exiting as well as a bit out of control in the style of a rollercoaster, once you get on - you have to do the ride. All in all amazed with the vigor, though clearly, the XLs outperformed the Forbiddens in growth. I have so many conditions going on, however, that all will be too close to call for now and in the long run my results will have been tainted by my experimenting and finding all optimal with these rocket-style plants. I'm amazed at this growth as Jack must have been when popping his magic beans. Still, they keep developing and power through with bud formation. I have to watch these lights, the SP 3000 by Mars Hydro is so powerful, that also getting this light optimal is a whole learning curve, but it does deliver a punch. I think I overdid the amendments at the start with the blood meal and that's why I see ridging so I'm careful with the Nitrogen and give them lots of fresh water with just some topmax in between. They seem to hold out but its on the edge. As far as bending, after the initial bend, I saw many branches develop that are nicely outward, tucked some leaves but I'm happy with the shape of the plant and will let the center bud develop. Take care and see you next week! Thanks for visiting!