SPREADSHEET: Jack's vs. Advanced: https://docs.google.com/spreadsheets/d/1HtHnZOxMHtBK5DFQjpBun2lbhDxYVhI7lJaq4W6xbyk/edit?usp=sharing 10/5 - Day 44: Things are good. Trellis netting is working and I'm spreading the canopy pretty well. We'll see what happens! I would recommended against the actual net I purchased, this shit is bulky, big and too stretchy.

She is plumped, ready for flush. Will start to feed plain ph water this week to deplete remaining nutes.

Hello everyone! Things are growing great! The scent is becoming more like weed and less like trash. Every time I check in on her she seems to have grown. Since I harvested my other girl last night, my six shooter has the whole tent to herself. Thats 3 fans, 2 × galaxy hydro 300 w leds, and an increase in airflow and drop in humidity. One week from today she will be much bigger. I have been feeding her every other watering. Got some nute burn but she seems to like the heavy feeds. I may go water,water, and then feed until harvest . I'm guessing between 2-4 weeks left. I can't wait to grow this strain again!

i let her roots build up on the first couple weeks after germination in a 1/2 gallon pot. 250ppm.

1/12: impatiently waiting for lemon auto to finish up. Still not sure what the northern lights is trying to do, but..the little buds are slowly growing. S l o w l y... 1/14: things are getting out of hand in here. Northern lights is bushy AF again but the budlets appear to be increasing in size. Lemon is getting closer every day. Looks like I've got milky trichomes and the pistils are darkening. Still a bit early, but I'd be lying if I wasn't anxious for the space.. 1/15: I've accepted that I have at least a week ish left on Lemon auto,but she's getting there! I did some rather risky defoliation on northern lights and holy shit, she's got potential. I'm hoping she's just a slow grower and those buds will really fatten up! Perhaps lemon is just a really fast growing plant? I've also decided that since space is limited, my best bet is to probably rotate the plants every other day to allow all bid sites an opportunity for direct light. I'm reigning myself in. Once lemon auto is harvested I need to NOT immediately plant another. I've got some mephosito seeds I want to get going, but I've learned my lesson. This tent fits two 5 gallon mature plants... Uncomfortably. Three is too many 😂 1/17: wondering if northern lights has is it her to fatten up. Gave her a good flush &feed. Popcorn buds or not, I'm invested (#forscience). Lemon auto is still finishing up. Can't get good enough pics with phone to see trichomes. Clearly, I have a minimum of a week left, but I got a digital microscope so I can keep track! Should be here for next update.

Hello growmies 👩‍🌾👨‍🌾🌲🌲, 👋 Let's go for the flowering, stretch Started, hope can stay under control, we can start to see the first pistils, Plant look happy. Weather start to be really cold, and worst next weeks, we'll see how turning. 💪 Keep Working on the canope, removing leaves and under buds site. 🕑 Now 12/1 💧 Give water each 2/3 day And vaporise plant with water + Plagron Roots (1ml/l) 1.5 l Water + Roots + Bloom + Zym + Sugar Royal (1 + 3 + 1 + 1 ml/l) PH @6 💡Mars Hydro - FC 3000 50% 43 cm. Mars Hydro Fan kit Setting 5 Have a good week and see you next week 👋 Thanks community for follow, likes, comments, always a pleasure 👩‍🌾👨‍🌾❤️🌲 Mars Hydro - Smart FC3000 300W Samsung LM301B LED Grow Light💡💡 https://www.mars-hydro.com/fc-3000-samsung-lm301b-led-grow-light Mars Hydro - 6 Inch Inline Fan And Carbon Filter Combo With Thermostat Controller 💨💨 https://www.mars-hydro.com/6-inch-inline-duct-fan-and-carbon-filter-combo-with-thermostat-controller Anesia Seeds - High Mars 36% THC🌲🌲 https://anesiaseeds.com/product/high-mars/

3/11/2023 Week 4- Day 1 of Veg (Day 38 overall)| As a precaution I changed out the water in the system today at the 1 week mark to try and make sure I am giving the most to the roots to ensure a healthy successful grow. Gallons into the system = 36 Gal Mammoth Silica =.5 Mil/Gal = 18 Mil Root Drip = 1 Mil/Gal = 36 Mil Cal Mag = .5 Mil/Gal = 18Mil FloraMicro = 4.2 Mil/Gal = 151 Mil FloraGrow = 3.8 Mil/Gal = 137 Mil FloraBloom = 3.0 Mil/Gal = 108 Mil ORCA = .5 Mil/Gal = 18 Mil I checked my lighting My target is 300-350 for this week. I will bump it up to 400 on Week 5. PPFD = 351 Tent Conditions when I opened the tent I was at 59% humidity however I had the doors open for an hour while I changed the water out and re-mixed Nutes so it was at 48 when I closed the doors but I have a Firebird controller which is set for 60 to bring it back up. Water Temp on Change out = 69.4 PPM= 514 PH= 5.93 3/12/2023 Week 4- Day 2 of Veg (Day 39 overall) Everything is going good so I decided to de-foliate some. 3/13/2023 Week 4- Day 3 of Veg (Day 40 overall) Humidity at 61% Tent Temp at 78 Water Temp at 75.3 PPM=574 PH 5.89 Happy, Happy... I topped 2 nodes on Plant #3 and 1 Node on Plant #2. Everything looks dialed in (Finally). I will be changing out the water this weekend and as long as everything looks good I will go back to only changing out the water once every two weeks. 3/14/2023 Week 4- Day 4 of Veg (Day 41 overall) Not much to report.. I defoliated a little bit today and took a couple tops off each. 3/15/2023 Week 4- Day 5 of Veg (Day 42 overall) Nothing to much to report. Took a few leaves today and a couple of Nodes topped on each of the plants. Everything seems to be dialed in for now. 3/16/2023 Week 4- Day 6 of Veg (Day 43 overall) Took a few leaves today and a couple of Nodes topped on each of the plants. 3/17/2023 Week 4- Day 7 of Veg (Day 44 overall) Last Day of Week 4. Water change tomorrow and it looks like I will be back to only having to chance the water every 2 weeks. Here are the end of the week Conditions. #2 10 1/2 Tall 20 Wide #3 11 Tall 21 Wide Water Temp= 74.8 Tent Temp= 78.3 Humidity = 61 PPM = 586 PH 5.80

after 20 weeks. The weather is wet and moldy, pests and diseases, so I took my scissors to harvest the plants I hope the weather can be better, but I can finally harvest mangoes, too long. Trees take a lot of time to VEG, with experience learning through social networks (Tea brewed) The varieties yielded much higher than normal, this is my best harvest. 2kg of fresh flower

Esa familia, a que pintan fenómeno estas skunks?. Es que Zambeza hizo un gran trabajo con esta variedad, es una cepa que es bastante rápida en floración, crece muy bien en su etapa de crecimiento, y sobretodo es aparte de típica, muy sencilla de cultivar. De mis favoritas hasta ahora para indoor, como siempre, controlamos el ph y la humedad (siempre dentro de los parámetros correctos establecidos). La semana que viene nos vemos con más avances , un saludo.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Welcome to my Pablø Ęscøbar diary. In this diary: Seeds: sponsored by Ðivine Șeeds Media: Promix HP Nutrients: Advanced Nutrients, Diablo Nutrients, Gaia Green Power Bloom. Light and Weather: Şun☀️and Mother Earth.🌎 ___________________________ Feeding: Tue 10Sep: 4L water not pH'd Wed 11Sep: 4L water not pH'd Thu 12Sep: 4L nutrients pH'd 6.5 Fri 13Sep: 4L water not pH'd Sat 14Sep: 8L water not pH'd Sun 15Sep: 8L water not pH'd Mon 16Sep: 8L water not pH'd ___________________________ *please note that watering are from the top.....since i smashed the saucers with the weed wacker*🤦🏻‍♂️ ______________________________ Amazing weather throughout the week!☀️Sunny 28°C everyday😎 ______________________________ Buds are rock solid almost a ping-pong ball size and she reeks really bad😍 Almost there! ______________________________ Thanks for stopping by, likes and comments are appreciated!👊🏻😎 Keep on growin! Keep on tokin!!! 😙💨💨💨💨💨

Week 9 commence flushing. This lady is looking great, super frosty, sticky and got a sweet aroma. Removed some dying leaves and now we wait for chop in about 6-7 days.

Number 2 pheno might herm on me so I have to watch but I have limited plants and I really want to keep these genetics so I need atleast one female. Number 3 the mutant stopped growing before I topped, or maybe not stopped but it threw out pistils at the apical meristem and not producing leaves so I topped to see if I could promote side growth but it hasn't worked; I'm going to keep her around just for the fun of it. 4 is my hope of producing female and a good clone.

Nice week, plants got higher 15-20cm which is nice, also many new budlets appeared and thats even nicer 😊

Belle couleur gorilla sherbet#1 sens très fort.

Ladies and gentlemen cultivators. It begins with the rinsing of our roots to remove all excess nutrients from the gems. Happy and abundant harvesters for everyone.

Hey Guys! Super excited this week! I've really been wanting to try growing outdoors, so I built a cheap hoop house! Got most of this stuff for the frame from Lowes under 300$. All the soil products I got from GrowGreenMI. Some really cool people out there. One thing I'm super worried about is drainage. Right now those holes are about 4 feet deep and they've got about 4 inches of water in them already. What's been cautioned is that, eventually, about 2 months in these plants roots will grow and reach the bottom and cause root rot to form. One of the biggest things I was trying to be wary of was causing root rot. This was also the biggest precautionary measure I took when mixing the soil. Adding the extra perlite, coco, and clay pebbles. Best advice right now is to build the soil up on the holes another 16 to 18 inches and possible stick a PVC pipe down to the lowest drainage point of the hole to allow some of that natural occurring water to evaporate. Any advice anyone has on it is welcomed! Making a compost tea for the soil outdoors, will probably put 2 cups in each RDWC bucket as well and let that do it's magic for a day or so before nutrient change. Raised the bed about 14 inches as well! All the seeds sank! Off to a good start! Lol

Got these girls started using the Vulcan Mind Meld technique! 👍👍