Week10: 500-400ml 18hrs Light! (RastaJeff) Loud loud loud

Vorblüten. Insgesamt wirken sie gesund, mit kräftig grünen Blättern und ohne Anzeichen von Mangel oder Stress. Die Beleuchtung läuft nun im 12/12-Zyklus. Der Abstand zur Lampe wird regelmäßig kontrolliert und angepasst, um Verbrennungen zu vermeiden. Die Temperaturen liegen tagsüber bei etwa 26 - 28 Grad, nachts bei 18–20 Grad. Die Luftfeuchtigkeit pendelt sich bei etwa 45 bis 50 Prozent ein. Die Pflanzen werden alle zwei bis drei Tage nach Bedarf gegossen. Diese Woche gab es die erste Gabe Blütedünger mit reduziertem Stickstoffanteil und mehr Phosphor und Kalium. EC- und pH-Werte liegen im optimalen Bereich. Ein Schädlingscheck verlief ohne Befund. Alles läuft reibungslos.

Die Woche verging wie im Fluge. Purple Punch Auto glitzert in den Morgenstunden wunderschön und verströmt einen sehr angenehmen Duft. Momentan duftet diese hier von unseren vier Pflänzchen am stärksten. Die Blüten schwellen nach einem krassen Stretch an. Das intensive LST und das Entfernen störender Blätter hat dazu geführt, dass die Blüten sehr gleichmäßig Sonnenlicht bekommen und später in der Blüte die Luft gut zirkulieren kann. Zwei Tage der Woche standen die Pflanzen unter der Markise, es hat über Stunden heftig geregnet. Nachdem ich meine Grashüpfer letzte Woche umgesiedelt habe, der Fraß ging mir zu weit, sehen die nachwachsenden Pistillen wieder gut aus. Vorher wurden die von den Hüpfern gerne angeknabbert. Das Gießen läuft wie gewohnt nach Gefühl. An heißen Sonnentagen bis zu 1,5 Liter. Soweit die News von unserem Balkon, die nächsten Wochen werden vergehen und dann ist die erste Saison als Weed Gärtner auch schon wieder beendet.

Perfect conditions this week , a touch too wet as some perlite is turning green but I’m repotting in a week so shouldn’t be an issue . Keeping heat mat on in off cycle to maintain temps and humidity. These girls are off to a flyer

My Sticky Rice plant is still quite small and hasn't shown a ton of growth yet. It's definitely the runt of the litter, but I'm hoping it'll pick up soon and fill in some of that extra space in the tent. Fingers crossed! Okay, This past week has been absolutely fantastic! The weather has been incredible, and I've been able to keep the windows open almost the entire time. My plants are thriving in the humidity, and the VPD has been perfect. Everything just feels so balanced and in check.

Added co2 to tent, added molasses and big bloom to nutrients for pyramid purples. Looking to veg For one more week. Reminder my diary is from seedling so veg is more like 6-7 weeks instead of 8. STAY TUNED. Youtube search “ nugg man95” need ideas for videos!!

3/16: Rain let up for half the day, but more tonight...damnit... They are due for a watering tomorrow, but with the high RH, maybe not... 3/17: They weren't ready for water yet, so I slept for a few bonus hours! (rare) Both of these plants continue to pack on the pistils with no signs of slowing down...I thought surely the shortie would be ready to flush this week, but she's not even close to ready yet.😕 It rained for most for the day....again... 3/18: I watered them with about 1/2 gallon each including terpinator, signal, sweet & sticky, cal-mag, armor si, endoboost, humic acid, and a little cha ching. It rained all night again..and most of the day today. I took some photos with the qb's turned off and a 135w 5500k CFL on.. 3/19: It rained all night and several times again today...ffs....I've started building an Ark in my free time 😁 3/20: Rained again on and off today..RH still too high... 3/21: I took the shortie out of the closet today to inspect her trichomes. I took about 2-dozen close-ups and zoomed in really close on my PC and couldn't find a single amber anywhere...and prolly only about 30% cloudy at this point.. I went ahead and took some cuttings from her while I had her out. Hopefully I can get at least one to survive and grow outdoors this season. I also took some cuttings from the FFT#6 (the other shortie), and I started a couple of other FFT's (5 & 7) which I'll train out as horizontally as possible to match the shortie's heights. 3/22: Rained again last night several times...and again today for a couple hours...ridiculous. Woke up with RH at 65%!!! Been chatting with other FFT growers and they all seem to be losing a lot of produce to botrytis...I'm starting to freak out..😳

I have a few strains in my tent at the moment but as it's my first diary I will be sticking with the one strain which is sweet deep grapefruit by dinafem seeds. Into week 8 we go, last week before flipping to 12/12, plant looks healthy and seems to be thriving using remo nutrients at the exact dosage recommended, only issue is new growth seems to be a little skinny an twisted at first but then sorts itself out any ideas why this could be? Humidity has been a little low past week but as I'm flipping soon I'm not to bothered by that. Give plant a little defoliation last week doesn't look like I touched her thhe way she bounced back lol. So far so good happy with the progress she's making :)

Week 4’s in the box and these plants are lightning quick outta veg. We’ve confirmed the presence of at least 3 males and 1 guaranteed female so far. They’ve all started to hit vegetative maturity in full stride so it won’t be long until we look to flip. A couple have been topped once more for the sake of additional colas and if we get lucky, the remaining females may be suitable for a scrog. Either way, can’t be much happier about how well they’re growing at this stage. Here’s hoping it remains so through flower🤞. ***Notes: strongest plant to this week is first confirmed female. Prob a prime candidate for cuts and back pollination with the F1 pollen dump.***

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Hi Grower! Complete 37 days Flower 08 days (7 nights) Today she shows her Gender! And im happy to can sure now that we have a girl. She is a little bit bitchy to grow, i think i got a big strech in the next days... Budspencer420 for 20% on www.bionovanutrients.com and ~5% on MarsHydro Viparspectra

It’s now showing pistils pretty well, and I got some critical peyote from Barney farms In a napkin right now There is a little burnt tip on fan leaves I think too much nitrogen since it’s in the big pot and the leaves are dark green. But looks good happy I picked the internodes during preflower too

Took some clones & defoliated/lollipopped I started building a veg tent and went with a 2x2 AC Infiniti equipped with the 4inch cloud line duct system was a boring grow week.. ready for flower

Beginning to get into her stride, and rather larger than the 20:1 CBD auto plant growing alongside her.

Plantitas sanitas...

Another week almost over, the girls are beautiful and smell great. Apparently everything is under control, I'm really looking forward to the results. Happy growing! 🌱🌱🌱

we had to go indoors everybody I don't know why its so fucking hot here in california evry fucking summer but the great indoors will do I guess with some cold ass a.c.

Took some clones 1 week before flipping to flower but forgot to take pictures. The video of the clones are from oct 1st. The Turnt smelled like chocolate cover strawberries but now are smelling of garlic and pepper with a bit of undertone of strawyberries. The Diesel smelled like lemon pinesol but now smells of citrus mixed with gas. The Banana Clipz smells of berries mixed with skunk. Paradiso smells of citrus mixed with fruit. Lung Buster smelled lightly sweet at first but now smells like berries mixed with a undertone of garlic,funk, and gas . Spilt Paint smells of berries mixed with diesel and funk.

03.08.21 Moin Growmies hab mir in der letzten Woche Gedanken gemacht zu meinem Vorhaben mit den Tomatenringen und mir ist aufgefallen das die Ladys nicht Grad gleichmäßig wachsen ,das liegt sicherlich daran das ich Depp wieder schlauer sein wollte und ein paar Seitenäste dran gelassen habe sprich beim ersten topping hätte ich alles entfernen sollen bis auf die 2 Triebe von der 3. Nodie und ich hab die 2 nodie mit stehen lassen weil ich dachte ich bin schlauer 🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬🤬ich könnte mich selber schlagen für die Dummheit naja Ergebniss des ganzen ist ungleichmäßiges Wachstum somit kann ich die Sache mit den Tomaten Ringen vergessen.......... Hab jetzt aber mein System noch einmal überarbeitet andere Topferhöhung ( 2x alte Bilderrahmen aus Holz auf Paletten Balken geschraubt und mit Hühner Draht bespannt das ganze auf Steine gestellt damit ich ne Wanne darunter stellen kann für abtropfwasser) den Rahmen vom scrogg netzt wieder raus gesammelt und neu bespannt ( Rahmen 90x90 cm aus nem alten Pavillon Gestell zurecht gesägt und mit Zaun bespannt die Abstände zwischen den einzelnen Rauten beträgt 6 cm sollte ausreichend Platz für die Triebe sein) habe das ganze gestern auf einer Höhe von 16 cm über dem Topfrand eingebaut mit Kabelbindern an den Zeltstangen befestigt somit hängt es an seiner Position fest und kann nicht von den Pflanzen hoch gedrückt werden wenn sie in die Blüte geht. Also is nun doch wieder ein sqrogg draus geworden aber den Ladys geht es insgesamt gut sie wachsen und gedeihen und wirken insgesamt gesund in den nächsten Wochen werde ich nun die Ladys in das netzt weben und Mal sehen was sie von scrogging Alla Milchschnitte halten. Ach ja die purps aus dem Garten sind anscheinend direkt nach dem umtopfen in den Stretch zur Blüte gegangen war ein bisschen spät dran fürs raus setzten aber lässt sich jetzt nicht ändern Mal sehen was draus wird,vll ja ein Gummibärchen Baum 😏😉 hab den outdoor Ladys gestern nen kleinen Regenschutz gebaut, is nicht der schönste aber er sollte seinen Zweck erfüllen damit der Topf Mal abtrocknen kann das Wetter ist seit fast 14 Tagen hart wechselhaft und der Topf noch nicht 1x richtig trocken gewesen das war's eigendlich was bis heut passiert ist cu Growmies und euch noch viel Erfolg........