🐩👤Sirius Black👤🐩 by 🌍🌱WeedSeedsExpress🌱🌍 👤 10.5 ... 👤 11.5 ... 👤 12.5 .... 👤 13.5 ... 👤 14.5 ... 👤 15.5 this week only simple photos, waiting for new big updates. yeah!!! 👤 16.5 ... 👤 17.5 Last days also for her finally, I can't wait to smoke it !!!! __________________________________________ ❓ Are you new to the world of the cultivation and don't know where to safely buy your seeds? 😮From Weedseedsexpress you order the highest quality marijuana seeds possible. Orders are processed professionally, with a personal approach and with respect for both your privacy and your needs! Use the code "LUCKY5FEM" to get an extra 5% discount on your purchases! ✅http://bit.ly/Weedseedsexpress _________________________________________________________________ 👀 Are you looking for a good lamp to start with? 👀 🌞Viparspectra has something more than the others, take a look at their site. ✅ http://bit.ly/pro-seriesproductspro-series-p1500 ⏩Use "GDVIP" for an extra discount, or "DREAMIT" for an extra 5% discount💯 👀Search for it on Amazon ✅http://bit.ly/ViparSpectraXS1000Amazon ⏩ Use the code - lt10gdvip - for an extra 10% discount 👀 Watch my ViparSpectra XS1000 unboxing on YouTube, leave a like and write to the channel 🦄 ✅http://bit.ly/UnboxingViparSpectraDreamIT _____________________________________________________________________________________________________________ 📷🥇 Follow the best photos on Instagram 🥇📷 https://www.instagram.com/dreamit420/ 🔻🔻Leave a comment with your opinions if you pass by here🔻🔻 🤟🤗💚Thanks and Enjoy growth 💚🤗🤟

Que pasa familia, vamos actualizar la cuarta semana de floración de estas Royal gorilla de RoyalQueenSeeds. Tienen buen color y ya van progresando esas flores, encontré algunos plátanos pero a estas alturas es ir retirándolos y con suerte se podrá aprovechar todo y estará rico pero seguro se folla alguna parte. Máximas de 28 y mínimas de 25 ahora viene el calor y ando jodido en humedad ronda el 50%. Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Hasta aquí es todo , espero que lo disfrutéis, buenos humos 💨💨

Was a good learning experience. I'm not going to get near the weight I expected from these, and though the bud turned out really tasty, I don't think I'll grow it again. I'm not sure I would recommend to a newbie. It was in some awesome living soil, but some deficiencies crept up in flower, and I wasn't impressed with the roots upon post mortem exam. On second look, even the photo on msnl's site shows some spots that looks like deficiencies. Definitely some grower errors here, but all of my ongoing grows faced the same challenges and these just seemed less adaptable. Anyhow, I've been graced with some clean meds for now, and I'm a happy growin hooman. 👽 I also probably won't do any more autoflowers moving forward. Just not my style. There are some great peeps doing outstanding work with autos though. Best to them and all! :) D82 - 12.12 - cut the second auto cheese yesterday. Let her go as long as I was comfortable. Think her run in with the gnats resulted in the the deficiencies. Awfully covered in trichs, so it should make for tasty smoke after dried, trimmed and cured.. Shall update

Bang, Bang!

Day 50 1/3/25 pistils turn amber buds get thicker guess 2 more weeks from now until harvest D54 1/7 feeded @ph6.5 and 1.2 EC with Greenhouse Short Flower

Thanks again to Humboldt Seed Company for AMAZING genetics. I can’t wait to grow more of her.

17.11. Day 43 Blüte 8 Hallo. Sie ist jetzt die Größte im Zelt. 20.11. HST in diesem Fall habe ich ihre Makkaroni dicken Äste und Stamm zusammen gedrückt bis es Kracht und splittert. 21.11. Invasion der Thirpse Teil 4.. Die Antwort: Arnold Pyrolitenegger. 22.11. noch 51 Tage Blüte...(oh shit) 23.11. Pyrolytenegger. UND was ne Genetik. Der Stamm ist dick wie eine Maispflanze.

Start of week 3. Feeding a little more consistently. 1gal of the above every 3 days.

Legend Timestamp: 📅 EC - pH: ⚗️ Temp - Hum: 🌡️ Water: 🌊 Food: 🍗 pH Correction: 💧 Actions: 💼 Thoughts: 🧠 Events: 🚀 Media: 🎬 D: DAY, G: GERMINATION, V: VEGETATIVE, B: BLOOMING, R: RIPENING, D: DRYING, C: CURING ______________ 📅 D64/B27 - 18/06/24 ⚗️ EC: 1.0 pH: 5.2 🌡️ T: 26°C H: 80% 🌊 FLUSH 🍗 💧 💼 FLUSH first day 🧠 🚀 🎬 1 TL video ______________ 📅 D65/B28 - 19/06/24 ⚗️ EC: 0.2 pH: 7 🌡️ T: 26°C H: 80% 🌊 FLUSH 🍗 💧 💼 FLUSH second day 🧠 🚀 🎬 1 TL video ______________ 📅 D66/B29 - 20/06/24 ⚗️ EC: 1 pH: 6.2 🌡️ T: 26°C H: 80% 🌊 15 L 🍗 CalMag - Bloom A-B - Bud Candy - B52 - Overdrive 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D67/B30 - 21/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D68/B31 - 22/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 15 L 🍗 CalMag - Bloom A-B - Bud Candy - B52 - Overdrive 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D69/B32 - 23/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video ______________ 📅 D70/B33 - 24/06/24 ⚗️ EC: 1 pH: 6 🌡️ T: 26°C H: 70% 🌊 🍗 💧 💼 🧠 🚀 🎬 1 TL video

Week 12, Day 83 from seed and 3rd day of flower.....The 5 day of this week officially marked the start of the flowering season!! So I flushed both of these bad boys with ph'd water for two days and then today I switched them to 12/12. I gave them their first dose of bloom nutrients and then lollipoped them as you can see, pixs . Grateful no issues to report for the past 3 weeks have been trouble-free!! I'm going to leave the MH bulb in for an additional 2 weeks to prevent any stretching before switching to the HPS. But so far they are still loving their environment. I'm also in the process of keeping clones of fat banana and hulkberry while i try this new strain of Peanut Butter Tree.

At the 5 week point we are still a way off what they should be due to their waiting for me to get them settled but today they look good and better than they have all week. The megacrop is working the magic now and I am getting them into their NFT later today so I expect them to fly.

These girls are progressing absolutely beautifully. I'm excited to see as it looks like there might be a purple pheno as I've never had that one grow before. No issues and the flowers are getting bigger daily. I did a defoliation and lollipopped up to maybe the 2nd-3rd node. All in all Happy Growing.

Day 42 to 49 We are getting closer to an end. Is the second time I’m growing purple genetics and I can say that somehow look to be faster to finish their life cycle; I think the next week will be the beginning of flushing, so I will just give water at PH 6.5, without any fertilizers, till the end which I believe will be around day 65. Let me know what’s your experience with purple genetics and of course if you have some tips! Cheers 🍻

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Recien comenzando a tirar muchos pistilos indicando que comienza la floracion!

Strain: Kannabia Seeds – Apple & Bananas Grow Type: Indoor Pot Size: 3 Gallon Pots Medium: Peat Moss & Perlite Nutrient Line: Athena Week 6 Update The Apple & Bananas girls have really shot up and are looking beautiful right now. All three are showing some nice healthy growth, but what I'm loving most is the shape they've developed from the training. Opening up those branches earlier definitely paid off. The canopies are spreading out nicely and they're developing the kind of structure I was hoping for. With plenty more veg time ahead, I'm going to keep working with that shape and let them continue filling out. There are no deficiencies or issues to report, so I'll stick with my regular Athena feeding routine. I may also look at transplanting them into bigger pots soon as they continue putting on size. So far, these girls are giving me exactly what I want. Let's keep them growing. Feeding Volume: 3L per plant Athena Grow: 45ml/gal Athena Core: 25ml/gal pH: 6.2 PPM: ~700–800 ppm Frequency: As needed Indoor Climate Conditions (Vegetative) 🌡️ Temperature: 20–25°C 💧 Humidity: 60–65% 📈 VPD: ~0.8–1.0 kPa See yal nex week!

Had a panic attack this week!!! Found some little fast moving crawlers on my mulch. Most were on the leaves I had trimmed off during defoliation. I come to find out they are a type of mite that eat the decaying foliage and were likely already within the soil itself. Luckily no problem at all. Lots of new growth and they all seemed to respond well to the little LST and HST I’ve used (topping and fimming). Almost daily I come in and press the tops out or down to help spread the canopy before trellising. The purple queen is truly the most impressive lady in the garden at this point. The Citradelic Sunset is a close second. Both plants are expressing 11 finger fan leaves and have very thick branches. I’ve had to raise my lights to 60” and everything has responded very well. They received about 4 cups each of compost tea this week. I backed off my watering schedule to help with the soil saturation.

The buds are spread everywhere and they are nice and frosty in next 2 weeks she will get fat.