Greetings, green enthusiasts! Welcome to another exhilarating update on the growth journey of my P.C.R.s. Buckle up, because things are about to get exciting! I'm thrilled to report that our P.C.R. plants are thriving in their third week of veg like never before. Their vibrant green hues are a sight to behold, and I can't help but marvel at their progress. But the real magic happened when I transplanted them into their final pots—the roots were an absolute spectacle, white and luscious, like something out of a dream. Aptus Holland, you've outdone yourself once again! Speaking of Aptus Holland, let's talk about the secret sauce behind this green revolution: the Aptus Holland products. My love affair with Aptus Holland runs deep, and it's all thanks to their innovative and effective plant nutrition solutions. When it comes to nurturing my girls, nothing else comes close. Let's start with the star of the show—the Aptus All-in-One pellets. These little powerhouses are packed with essential nutrients, providing a balanced diet for my plants and ensuring they have everything they need to thrive. Combined with the Mycor Mix powder, they create the perfect symbiosis, enhancing root development and nutrient uptake to levels that even I couldn't imagine. But that's not all—I've also added a touch of Aptus substrate buffer powder to the mix, ensuring that my super soil recipe is dialed in for maximum performance. With this concoction, I'm confident that magic is bound to happen in the days to come. Of course, I can't forget to mention the Aptus Holland Veg Mix, which continues to be my go-to solution for watering. With each application, I'm providing my plants with a carefully balanced blend of nutrients, setting the stage for vigorous growth and robust health. Now, let's talk numbers. My TDS is currently hovering around 370, while the pH remains a stable 5.8. And with temperatures holding steady at 21 degrees, the conditions couldn't be more perfect for our green darlings to flourish. Before I sign off, I want to extend heartfelt gratitude to Aptus Holland for their unwavering support as my main sponsor. Your products are the backbone of this operation, and I couldn't do it without you. A special shoutout to Art Genetix for bringing the incredible P.C.R. creation into existence, and to Grow Diaries and the entire community for your endless inspiration and camaraderie. Here's to another week of growth, discovery, and green magic! Stay tuned for more updates as we continue this green journey together. And as always, a heartfelt thank you to Aptus Holland for providing the nutrients that make it all possible. Here's to another week of growth, discovery, and endless possibilities! Genetics - P.C.R. @Art_Genetix_Team https://artgenetix.world/ Nutricion @aptusholland https://aptus-holland.com/ LED Power @Lumatek and @viparspectra As always thank you all for stopping by , for the love and for it all, i fell blessed to have you all with me for one more love journey Thank you Thank you Thank you , you guys are great and have been amazing , thank you for everything ! #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #dogdoctorofficial #growerslove
 With true love comes happiness , Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so ! Growers love to you all

Hi Like u all saw I deleted my mix max the finest diary, due problems and not being able to give each strain enough space and time on here !! So now Iam concentrating on just a few selected strains This diary is about the Trainwreck by Humboldt seeds Infos : Trainwreck is a mind-bending, potent sativa with effects that hit like a freight train. Mexican and Thai sativas were bred with Afghani indicas to produce this Northern California staple, passing on a sweet lemon and spicy pine aroma. Trainwreck begins its speedy hurtle through the mind with a surge of euphoria, awakening creativity and happiness. Migraines, pain, and arthritis are mowed down by Trainwreck’s high-THC content, and many patients also use it for relief of anxiety, ADD/ADHD, and PTSD. Trainwreck flowers tend to finish early when growing outdoors, while indoor gardens are ready for harvest after 8 weeks. I got the cutting at the 03.12.2019 due too stress due transport and time issues it stand in the shadow for almost 10 days and there wasn’t much growing going on ! The motherplant is about six month old and it was selected by a friend between 20 regular seeds !! This pheno Type it’s growing really bushy and doesn’t strech too much about a 1/3 of original starting highs When well trained and cut Day 1 - is 16.12.2019 and I count that after I took cuttings from the top and transplanted the freshly rootet plant from a 1/2L into a 4 L pod !! Day 2 - still boring stuff going on for about a week no water today Day 3 - no water Day 4 - no water Day 5 - no water Day 6 - no water

Flowering day 68 since clock change to 12/12 Hey guys :-) . It's so far, the lady is ripe and will be cut at the bottom end in the coming days and hung upside down in the drying tent :-) . Next week there will be an extra update on how they are hanging. Watering would be done twice this week with 1 l each (nutrients see table above) Otherwise it was checked and everything was cleaned. Have fun and stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this Nutrients at : https://greenbuzzliquids.com/en/shop/ With the discount code: Made_in_Germany you get a discount of 15% on all products from an order value of 100 euros. 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : Clearwater Seeds Water 💧 💧💧 Osmosis water mixed with normal water (24 hours stale that the chlorine evaporates) to 0.2 EC. Add Cal / Mag to 0.4 Ec Ph with Organic Ph - to 5.8 - 6.5 MadeInGermany

Day 40 ( 3 in flower) : These girls are amazing and deserve some star status for their growing ability. From the off these girls have given full attention to their growth , even the 2 girls that were suffering under the intensity of the lights ( moved to 24 "now.) It gave 2 of them tip curl like too much Nitrogen does so had me head scratching on the new Mega crop and dosages. Now figured they are continuing to outgrow most of the other strains running. They have been easy to work with on their lst and pin down and far extend the pots edges. #1 The living organic girl is loving her spot in the room and paying me back in her spurts of growth. # needs a little thinning out but happy .#3 Is a willing bush in the making but a good defol will open her up.

Everything good except a bit of salt buildup caused by a poor runoff over the last weeks. You can see this by looking at almost every tip of the leaves. Last week Amazon had some problems delivering new ferts, so from day 97 to 101 I gave just 1ml/L of Bud Factor X and no Bud Candy and Sensizym at all. On day 101 I made a new nutes mix, and it’s the one you can see on GD. No more Bud Factor X because it’s very expensive and I don’t think I’ll need it for just two more weeks. I used 1ml/L of Cal Mag Xtra because I ended my Connoisseur Bloom Part A too. Lol, it’s been a problem. I ordered some Sensi Bloom Coco A+B and another Bud Candy for the last two weeks by the way. Flowers are now enlarging since the first day I added Overdrive. Very very good, I like this Silver Haze.

I'm 90% sure this one is in her finishing week here. More amber trichromes are coming in daily. I don't wanna push her too far like last time because this strain turned pure amber and kinda lost some crystaly luster during the curing process. My light timer, grow tent, and light upgrades really made a big difference in bud development compared to my last run with Runtz basically leading to bigger more frostier buds. I'm dropping temps lower at night. I give it a couple liters a day to keep the top soil moist. Flushed water comes out 6.8 ish. My CO2 exhale bag was the biggest flop as it was expired and did not activate. I plan on 48 hours darkness before harvest starting today: the day of the eclipse. With the new moon we have a high pressure system that is peaking at night around 1028 dB. Water should be pushed up into the plant from roots. Coupled with lights out....all theoretical witch craft. I have a small 2x2 dark tent pictured which needs to have AC bring in cold fresh air from outside. If no fresh air added the tent gets humid and stagnant after a while. The darkness period is bringing out that strong and sweet 'medicinal' smell. If you ever tried weed labeled medicinal youd have an idea of what I mean. I guess thats the orange bud/chemo lineage traits. Before lights off the smell was more towards yeast fermentation smell and not soo sweet.

She seems to be recovering from the water log roots…after she dries out I will feed her some fish fertilizer…in case this is a nitrogen issue.

Looking good, besides the runt plant

Planta fácil de cultivar, de estatura media y producción importante de tricomas. Sus fragancias son dulces parecidas a la panela, canela, especias y un fondo un poco kush con notas a crema. Flores hermosas macizos, compactas y totalmente cubiertas de fragante resina, las tonalidades son hermosas, al moler se despliegan todos los aromas y ese tono violeta en el interior de las flores. Gracias a fastbuds una vez más por su confianza y a toda la gente que sigue el contenido. Buenos humos!

🌱 Week 1 Update: Terpyz Mutant Mentha de Croco Journey 🌱 Hey Grow Fam! The Mentha de Croco sprouts are officially a week in, and they’re already showing off their unique personality, each one growing at its own pace. They’re rocking some funky vibes and loving life inside the dome! Here’s a full update on their progress, care, and setup for Week 1. Living Environment: The Humidity Dome Setup The seedlings are living in a humidity dome, where we’re keeping the relative humidity (RH) close to 100%. This level of RH mimics a tropical environment, creating the ideal conditions for delicate young plants that need extra hydration support as they begin to develop roots and leaves. Why High Humidity? • Encourages Strong Root Development: At nearly 100% RH, plants focus on root growth while pulling in moisture directly from the air, helping them establish themselves early on. • Protects from Stress: The high humidity reduces transpiration (water loss through leaves), which prevents drying and keeps seedlings hydrated. • Promotes Healthy Leaf Growth: With constant access to moisture, the leaves expand smoothly, giving each plant a strong start in the vegetative phase. Growth Status & Observations 🌱 These little mutants are showing some funky characteristics and growing at their own unique pace—a perfect reminder that no two plants are the same! Each seedling has sprouted its initial set of leaves, and I’m seeing great promise in their vigor and resilience. Key Observations: • Color: The seedlings are a healthy green, with some displaying slight variances in shade—typical of unique genetics like Mentha de Croco. • Structure: Leaves are developing steadily, with short internodes (the space between sets of leaves) that hint at nice, compact growth patterns as they continue. • Energy: Each plant seems to be finding its rhythm, which is a beautiful thing to watch! Some are taking their time, while others are diving right into rapid growth. Nutrients & Supplements 💧 For this first week, I’m keeping it light on nutrients, letting the seedlings draw what they need from the Root Riots and the remaining seed stores. However, I’ve continued using Aptus Holland’s Regulator and Startbooster, both at 1ml/L, to support foundational growth. Why These Nutrients? • Aptus Regulator: Adds silicon to fortify cell walls, giving the seedlings extra strength to handle environmental changes. Even under the dome, this helps build resilience. • Aptus Startbooster: Supplies essential microbes and root stimulators, setting up these little ones for explosive root growth once they’re ready to venture outside the dome. Lighting: FOG LED & Environmental Control 💡 I’ve kept the Future of Grow (FOG) 600W LED dimmed to around 200 PPFD to avoid overwhelming these tender seedlings with too much light. They’re getting just enough energy to support healthy photosynthesis without stressing them in their early stages. Lighting Benefits at Low Intensity: • Prevents Stretching: With the lower PPFD, plants stay compact and focused on root and leaf development rather than reaching for the light. • Supports Early Photosynthesis: The FOG LED’s balanced spectrum provides all the wavelengths these seedlings need to kick off photosynthesis and grow with vitality. Controlled through the TrolMaster Tent X, the lighting schedule is consistent and stable. With this controller, I can manage humidity, lighting, and temperature settings all from one place, ensuring the environment stays perfect for these little ones. Reflection & Tips for Fellow Growers 💡 Week 1 is about creating a stress-free environment for seedlings to adjust and establish themselves. Here’s a bit of wisdom for anyone embarking on a similar grow: 💧 Humidity & Hydration: High humidity is a seedling’s best friend. Keep your dome or grow area humid for strong, happy plants. 💡 Light Management: Keep lighting low to encourage compact growth and let those roots dig in. Adjust lighting gradually as they grow to avoid any shock. 🌱 Patience with Genetics: Each plant has its own rhythm, especially with unique genetics like these. Let them show you what they’re made of without pushing too fast. Shout Outs & Community Love 💚 Much love to Terpyz Genetics for these incredible seeds, Aptus Holland for the nutrients, Future of Grow for the stellar LED, and TrolMaster for the environmental control. Big shout out to everyone following this journey—it’s always a blast sharing with you all. Let’s keep the positive energy flowing and watch these seedlings turn into something amazing together! Stay tuned for Week 2 updates—more growth, more funk, and more growers’ love! Genetics - Mentha De Croco https://terpyz.eu/products/menta-de-croco-fern-type?_pos=1&_sid=e9237cbcb&_ss=r DogDoctorOfficial Discount Codes @ terpyz.eu - DOCTOR 15% off Nutrition - @aptusholland https://aptus-holland.com/ Led Power @ F.O.G. Future Of Grow https://www.thefuturofgrow.com/en/online-store/BLACK-SERIES-600-p489093171 Controls @ TrolMaster https://www.trolmaster.eu/tent-x As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciloved and i fell honored with you all in my life With true love comes happiness Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so Growers Love To you All 💚

I think she has one week delay and is in week two of flowering

Week 8. They got their First flush. Look at those beauties. Better pictures by the end of the week.

Eccoci qui... Tutto va per il meglio, questa settimana non ho applicato stress alla piccola, ma ho guardato l'evoluzione che ha avuto dando i nutrienti. Ora ha un odore davvero intenso ed uno strat di resina pauroso, vediamo come si riprende dalla defogliazione che ho fatto settimana scorsa. Seguiranno aggiornamenti, grazie a tutti per il supporto🔥🌲❤️

As altas temperaturas arruinaram a colheita, as plantas estão sem cheiro a WW#1 foi a mais resistente ao calor, ainda exala um aroma muito bom, porem as outras plantas sofreram mais, a C99xBB#1 perdeu totalmente o cheiro, e nasceram novos pistilos diminuindo a densidade das flores.

Welcome: thanks for dropping by. Be sure to drop a like so I know u dropped by and I can then check out your grows. Or not, I'll leave that down to you but either way good luck with your grows, stay safe. 28/4/22 Have started these on the voodoo/La Calvera. These versions are from the ppl who hold the pattens and make a lot of ANs formulas. They have just improved upon most of the AN line up but they kept these the same. As they are tried and tested. You can check these out on led-grower.eu/terra-power/ and read up on them for yourself. Going to be running this one differently. As I can only use Terra Power Nutrients that match what AN products I have to make it fair, as it's a comparison grow I won't be using the 50 billion microbes per 1ml enzyme and a few other things. Have to make it as fair as I can. Everything will be the same, (only thing that will be different is the feed charts and how some of terra Power Nutrients can be applied as a foiler spray, I'm going to be following AN/TP grow charts to the letter to make it as fair as possible with decent results). Pot size, transplant/feed/light time Will all be the same. Hopefully soon you'll be able to click on them and look em up when they join GD in the next wk or so. As soon as both plants start to show leafs I will then apply nutrients in a very small dose. Like .5ml/l to start off with and of course I'll use the same amount of nutrients ml/ml. Hopefully the PPM won't go too high in TP as if it is I'll have to use less TP to match the PPM or get it close but if its not off by too much that it won't hurt the plant I'll be running with it. Maybe I'll add the nutrients solo when they start to feed. Both plants have been given 100ml of voodoo/La Calavera 300ppm 6ph 20c temp. 100ml and will apply again in 2 days. And when leafs start to show I'll start them on a weekly base nutrient feed but for waters in between I'll most likely be adding enzymes and root stims on every feed as they have very little EC and won't cause nutrient/tip burn. This is 1 of 6 so far that has been planted. 50% of the strains I have doubled up on, for this 1 though there'll be dif ph/ppm and that which I will add here. Advanced nutrients grow: everything I did above was repeated. PPM was 280 PH was 5.7 temp was 20c. When leafs show I will apply base nutes. I've laid a damp towel down around the pots on the ground to try and bring the RH up to 70+. (Don't fully wet the towel as it won't dry fast enough to make an impact on RH.) 30/4/22 Veg started on both ladies. VD1, both look very nice and just 38hrs in the ground they are both up. When I checked last night they weren't up but I had a feeling some would pop while I was sleeping, so I decided to turn on the grow light. I know it doesn't need to be on until they are up and showing the 2lil fan leafs but hey, 65% it is set at and 45cm away. RH is holding at 67% wet towel is just about dry will be wetting it again. Will also give both girls a feed after they come out of their rest period. As they are all so new I have turned the light on a 7/7 meaning every hrs it comes on and off. Will hold off on nutrients until I see both plants holding out hands. Will introduce some enzymes later today also. 1ml/L of Piranha and tarantula for both of them with voodoo. Both nutrient brands are the same, it's the same formula. Decided to make up the mix. Each custom terra P nutrient has a corresponding Adv Nutrient underneath. I'm going exactly of each of their grow charts and using as guide lines. Adv Nutrients says to use Tarantula&Piranha in weeks 1-2 of grow but terra Powers versions say to use these on weeks 3-4 So that's why I'm going to do. Ive used ml/ml in each nutrient. Adv Nutrients PH was 6.4ph and the EC was .94 TERRA POWERS nutrients were more stable and more potent PH 6.0 EC 1.19. So it looks like the PH balanced nutrients aren't controlled by the amount of ml you use as when you use 2ml/L of both grow A+B of adv nutrients the PH is in the range of 6.2. Water used was 6.7 our tap water has gone up must be because we coming into summer and the ppm jumped up from 78 to 105ppm. I use a charcoal filter for all my water doesn't change PH but lowers PPM from 78 to 105. I'll be using this 1L mix throughout it's 1st wk on every feed. As it's such a low dose I have no concern about salt build up. When I up the ml/L I'll use 1L of mix feed of each nutrient. So if the dose is 1ml/L they'll get that total amount in 1 week and as they get bigger and will be able to take 1l at a time they'll only be getting 1 feed of nutrients and water/enzyme feeds and root stims with no EC/SALT. 2/5/22 Both plants doing well. Fed again today. 100ml both plants their own nutrient mix. Both held the same EC but AN PH went up to 6.6 from 6.4 (still fine, and reasonable for a 3 day old mix) and terra power no change. 4/5/22 Made new mixes for both plants adjusted AN PH to 6, the same as Terra Power. (Needs no PH-+ ).. to make it as fair as I can, I've added a biobizz calmag agent to both mixes. 2ml/L was added to all 8mixes. 400ppm 2ml/L PH was 6.4 had to use PH - To bring it to 5.8. Didn't have any affected on the PH of terra power or the adjusted AN. I am guessing I only need a PH - now is cause I'm using so little of the PH perfect solution which is normally 6 but when mixed normally AN holds 6.2-3...Terra Power holds on 6 on everything so far. 6-7 nutrients all 6 PH. La Sirena is sooooo potent. Decided to add La Calavera aka voodoo juice. So, since I don't have a lux/ppfd meter. I use this to know exactly where it's most powerful. It really helped. As I had 4 plants is 12000 where I had space where the light was hitting 18500, I know the strength is off, but it gives me a guide as to where to place my plants it is going to help so much. 6/5/22 So, it's begun. I've created a negative pressure environment, 1st time, let's see if it makes an impact. On wk 3 onwards I'll be running the intake extraction for 5mins every 30mins and I'll have 2 co2 bags up. If the RH becomes a problem or temp I'll put the extraction/intake back on 100% of the time. The extraction fan/intake is set at a ratio of 2 to 1. (100% extraction 50% intake) making a negative pressure environment, best way to show you have done this, the walls in your tent should bow in lightly. In the beginning of flower in going to seal my 150x150 and I'll add 2 co2 bags into it and seal it up with the dehuimifier set at 45% 12/12 and it'll be superhot and I'll then put the photos into a negative pressure environment that'll be 8-10c lower and will have 45-50% RH, hopefully this will bring any terps out, the autos will get a 6hr rest in a negative pressure environment also this will bring the temp down by 8-10c and giving me a sudden drop in temp, let's see what happens. Same Day. Decided to brace AdvNutrient plant so she grows up right, she started to bend, had bout a 30degree bend. Simply braced her with a clamp to a stick a few cm away from the tap root with stem inbetween, should keep the ADVNUTE plant up right. Terra Power plant growing straight and healthy, she was only started on the root stim yday and no enzymes yet and she's not too far behind. I can see TP take the lead in the late veg-pre flower stage. Idk. But there's 2 major nutrients ADV plant is getting was 3 all week and then I added La Calavera (ANs voodoo juice) last night. Going to be running another AN/TP grow this time I'm not only going to match Nute for nute but the same time and ML/ML will be given also. Check out CBD CA for results. This will be the last update for this week. Thanks for reading gottagrowsometime

💩Holy Crap We Are Back💩 Well i had so much fun the last round , that we are going at it again 😁 So super happy to be trying this one out , can't wait😝 ...i have never done a purple before so here we go ..👍 Seed soaked for 24 then placed in a tub with paper towels , once it cracked and rooted , placed into its main pot 👌 👉I WISH GD HAD DISCORD SERVER SO GROWMIES COULD HAVE A PLACE TO HELP AND TALK THINGS RELATED TO GROWING .....👈 👉SO I CREATED ONE ALL YOU NEED IS TO JOIN THE GROWDIARIES DISCORD SERVER !!!!!!!!!!!👈 LINK IS 👉 https://discord.gg/zQmTHkbejs AND SEE HOW IT PLAYS OUT !!!!!!!

9/15 12AM MONDAY!!! THEY ARE ALL DOING GREAT!!! VERY HEAVY AMINOS YESTERDAY, NO DAMAGE. 9/15 11:24PM. In plant cells, glutathione S-transferases (GSTs) act as transporters, facilitating the movement of anthocyanins from the endoplasmic reticulum (ER) to the vacuole for storage, though their exact mechanism is still being studied. The synthesis of glutathione (GSH) itself occurs in the cytosol of mammalian cells through two ATP-requiring enzymatic steps, while anthocyanins are synthesized via a separate phenylpropanoid pathway 9/15 11:42PM glutathione metabolism glutathione S-transferases plantsThinkingKicking off 5 searchesLooking at 71 sitesPutting it all together In plants, glutathione S-transferases (GSTs) are a diverse family of enzymes integral to glutathione (GSH) metabolism, primarily functioning in detoxification and stress response. The core of this process involves GSTs catalyzing the conjugation of the tripeptide GSH with various toxic compounds, or xenobiotics, making them less harmful and easier to sequester or transport. Glutathione (GSH) metabolism in plants GSH metabolism is a tightly regulated process that maintains cellular redox balance and protects against stress. Biosynthesis: GSH is synthesized in two adenosine triphosphate (ATP)-dependent steps:(gamma )-Glutamylcysteine synthetase (GSH1) joins L-glutamate and L-cysteine to form (gamma )-glutamylcysteine. In Arabidopsis, this enzyme is located exclusively in the chloroplasts.Glutathione synthetase (GSH2) adds glycine to (gamma )-glutamylcysteine to produce GSH. This enzyme is found in both the cytosol and the chloroplasts.Oxidation and reduction: GSH exists in two forms: a reduced monomer (GSH) and an oxidized disulfide dimer (GSSG).Oxidation: In the process of scavenging reactive oxygen species (ROS), GSH is oxidized to GSSG.Reduction: Glutathione reductase (GR) uses NADPH to reduce GSSG back into active GSH, which is essential for maintaining a high GSH-to-GSSG ratio inside the cell.Degradation: Glutathione can be degraded in the cytosol, apoplast, and vacuoles by (gamma )-glutamyltransferases (GGTs) and other peptidases, allowing for recycling of its amino acid components.Transport and compartmentalization: While synthesis occurs in the chloroplasts and cytosol, transporters move GSH and its conjugates throughout the cell, including to the mitochondria, peroxisomes, and vacuoles. Functions of glutathione S-transferases (GSTs) GSTs are a large, ancient gene family with diverse roles that extend beyond detoxification. The primary functions involving GSH are: Detoxification of xenobiotics and heavy metals: GSTs catalyze the conjugation of the thiol group of GSH to a wide range of electrophilic and hydrophobic substrates, including herbicides, pesticides, and heavy metals. The resulting GSH-conjugates are then recognized by ATP-binding cassette (ABC) transporters and sequestered in the vacuole, reducing their cellular toxicity.Protection from oxidative stress: Stress conditions like drought, salinity, and high light increase levels of ROS, which cause lipid peroxidation. 🚨Some GST isoforms have glutathione peroxidase (GPOX) activity, using GSH to reduce toxic lipid hydroperoxides to less damaging alcohols, thereby protecting cell membranes.Defense against pathogens: During pathogen attacks, GST activity increases to detoxify microbial toxins and secondary defense-related compounds. This helps to bolster the plant's resistance response.Metabolism of endogenous compounds: GSTs play roles beyond immediate stress response by participating in the normal metabolic processes of plants. Examples include:Hormone transport: Some GSTs act as binding proteins (ligandins) that transport plant hormones like auxins within the cell.Metabolite transport: Specific GSTs facilitate the transport of important compounds like flavonoids and anthocyanin pigments into the vacuole.Tyrosine degradation: The zeta GST class (GSTZ) catalyzes a critical step in the breakdown of the amino acid tyrosine.Redox signaling: By influencing the GSH/GSSG ratio and interacting with plant hormones, GSTs can regulate cellular signaling pathways that control growth and stress response. 🚨GSTs and plant stress response The expression of many GST genes is highly inducible by various environmental and endogenous cues, making them key players in a plant's ability to adapt to adverse conditions. Abiotic stress: Drought, salinity, heavy metals, and extreme temperatures all trigger increased GST expression, which helps plants manage resulting oxidative damage. Overexpression of specific GST genes has been shown to enhance stress tolerance in transgenic plants.Herbicide resistance: The ability of crops and weeds to detoxify herbicides through GST-catalyzed conjugation is a well-studied example of GSTs in stress adaptation. Herbicide safeners, chemical agents that protect crops from herbicide injury, work by boosting the activity of these GST enzymes.Developmental processes: Studies have linked the expression of certain GST genes to specific stages of plant development, such as fruit ripening, root and stem development, and flower formation. "" 9/16 12:32am AI Overview In plants, phenylalanine is the precursor for anthocyanin synthesis, a process that involves a multistep pathway in which glutathione (GSH) plays a critical role in the transport of the final pigment to the cell vacuole. Phenylalanine initiates anthocyanin synthesis The biosynthesis of anthocyanins begins with the amino acid phenylalanine, which is produced via the shikimate pathway. The conversion process, known as the phenylpropanoid pathway, involves a series of enzymatic reactions: 1. Phenylalanine ammonia-lyase (PAL): This enzyme catalyzes the first committed step of the pathway, converting L-phenylalanine into trans-cinnamic acid. 2. Downstream enzymatic reactions: A series of additional enzymes, such as chalcone synthase and chalcone isomerase, convert the intermediate compounds into unstable anthocyanidins. 3. Glycosylation: Glycosyltransferase enzymes stabilize the anthocyanidins by adding sugar molecules, creating the final, stable anthocyanin pigments. 4. Glutathione is crucial for anthocyanin transport After synthesis, glutathione is essential for transporting anthocyanins for storage. * Glutathione S-transferases (GSTs): These enzymes bind to the newly formed anthocyanins in the cytoplasm. This binding is a key step that facilitates the transport of the anthocyanin-GST complex across the tonoplast (the vacuolar membrane) and into the vacuole. * Vacuolar storage: Once inside the vacuole, the anthocyanins accumulate and provide color to plant parts, such as flowers, fruits, and leaves. * Catalytic role: Recent research has also found that certain GSTs have a catalytic role in anthocyanin synthesis. They can catalyze a dehydration step to form specific anthocyanidins, significantly boosting production in engineered systems. * The process in summary The synthesis and accumulation of anthocyanins involve a coordinated process of multiple steps: 1. Production from phenylalanine: The amino acid phenylalanine enters the phenylpropanoid pathway, where enzymes convert it into the basic flavonoid structures. 2. Formation of anthocyanidins: Further enzymatic modifications produce the colored but unstable anthocyanidins. 3. Stabilization and transport with glutathione: These anthocyanidins are stabilized by glycosylation and then bound by GSTs (which use glutathione). This binding enables their transport into the vacuole for storage and visible pigmentation. 9/15 12:46AM https://pmc.ncbi.nlm.nih.gov/articles/PMC7238016/ 9/16 1:30AM BKO is looking great!!! Conclusion The “butter frosting” resin on Cookie Apple F1, healthy yellow-green fusiform, and Blueberry KO’s pigmented cotyledons show your anthocyanin-glutathione-phenylalanine strategy is working—phenylalanine drives synthesis, glutathione ensures transport. Tweak amino acids to 100–150 mg/L to reduce tip burn. 9/16 3:34am 9/16 4:31AM Anthocyanin glutathione synthesis phenylalanine proline tmg powder relating current project: * Phenylalanine is a precursor: Phenylalanine is an amino acid and the starting point for the phenylpropanoid pathway in plants. * Anthocyanin synthesis: This pathway creates various secondary metabolites, including the flavonoid pigments known as anthocyanins, which give plants their red, purple, and blue colors. * Pathway activation: Multiple enzymes, such as phenylalanine ammonia-lyase (PAL), catalyze the conversion of phenylalanine into the building blocks for anthocyanin. * Anthocyanin and glutathione synthesis * Glutathione S-transferase (GST): This enzyme is crucial for synthesizing anthocyanins in plants. It transports anthocyanins into the cell's vacuole for storage. * Glutathione (GSH) production: Anthocyanins can promote glutathione synthesis in certain cells. For instance, the anthocyanin cyanidin-3-O-β-glucoside (C3G) has been shown to increase the expression of glutamate-cysteine ligase (Gclc), a key enzyme in GSH synthesis. * Antioxidant effect: By upregulating GSH production, anthocyanins contribute to the antioxidant defense system, protecting cells from oxidative stress. * Proline and glutathione synthesis * Shared precursor: In some organisms, the synthesis pathways for proline and glutathione share a precursor molecule called γ-glutamyl phosphate. * Alternative pathway: Research on bacteria and yeast has revealed an alternative, trace pathway where γ-glutamyl phosphate from the proline synthesis pathway can be diverted to produce the glutathione precursor γ-glutamylcysteine. * Oxidative stress response: Studies in mouse oocytes show that supplementing with proline upregulates genes related to glutathione synthesis (Gclc and Gclm), increases glutathione levels, and reduces oxidative stress. * TMG powder, methylation, and homocysteine * Methyl donor: Trimethylglycine (TMG), or betaine, is a potent methyl donor, meaning it provides methyl groups needed for various biochemical processes in the body, including the methylation cycle. * Homocysteine regulation: One of TMG's primary functions is to convert the amino acid homocysteine into methionine. This helps regulate homocysteine levels, which is important for cardiovascular health. * TMG and proline interaction: In plants and some organisms, TMG and proline act as compatible solutes or osmoprotectants, helping cells stabilize against osmotic stress like drought or salinity. However, in human biology, TMG mainly functions through methylation, while proline is involved in different metabolic and antioxidant roles. * How they all relate The listed components are connected through several overlapping metabolic and regulatory pathways: * Anthocyanin synthesis starts with phenylalanine. * Anthocyanins can promote glutathione synthesis via upregulation of key enzymes like Gclc. * Glutathione synthesis can be influenced by the proline synthesis pathway, as they share an intermediate in some contexts. * TMG powder supports the methylation cycle, which helps regulate homocysteine levels. While TMG and proline serve similar protective roles in some organisms, their primary human metabolic functions differ, with TMG focusing on methylation and proline having distinct roles in antioxidant response and metabolism 9/17 217am Die Hard Christmas Grow 9/18, 11:34 AM. I ordered some square saucers that were cartoonishly too small but they fit inside the AC infinity germination kit and they fit with the Bud Cups perfectly really nice so it’s not a total loss. 9/18 11:45AM mix. Foliar Spray, the rest of the mix ec 0.46 Mixed up Aminos first and separate and use 16 oz for foliar spray. Then mixed up: Root: 1 mL/L Connoisseur A & B GROW, .2 mL/L CaliMagic, .2 mL/L Purpinator. Setria Glutathione: 150 mg/L(Brand: Emerald 250mg capsule.) TMG: 150 mg/L = (Brand Nutricost) Phenylalanine: 150 mg/L (Brand Nutricost) Proline: 150 mg/L (Brand Nutricost). 9/18 228PM AI Overview Glutathione influences plant colors by regulating the accumulation of pigmented compounds, primarily anthocyanins. The tripeptide accomplishes this through its role in transporting pigments within plant cells and in protecting against environmental stresses like UV radiation that can cause oxidative damage. Transporting pigments into plant cell vacuoles Glutathione works with a class of enzymes called Glutathione S-transferases (GSTs) to transport pigments like anthocyanins into the vacuole for storage. Anthocyanin transport: In plants with pigmented tissues, such as purple grapes or red flowers, glutathione-conjugated pigments are transported by GSTs across the tonoplast membrane into the vacuole. This process is crucial for the stable accumulation of pigments. Genetic manipulation: Research shows that manipulating specific GST genes can alter a plant's pigmentation. For instance, silencing a particular GST gene in purple grape hyacinths caused their petal color to shift to a lighter shade of purple. Similarly, defective GST genes in carnations resulted in pale-colored flowers. Protecting against UV light and stress Glutathione helps regulate plant pigmentation in response to environmental factors, especially UV-B radiation. Activating flavonoid production: When plants are exposed to UV light, a surge in glutathione triggers the expression of genes involved in producing flavonoids. Flavonoids, including anthocyanins, can act as protective sunscreens for the plant, and their increased synthesis and accumulation can alter visible coloration. Balancing oxidative stress: Intense UV-B radiation increases reactive oxygen species (ROS) in plants, which can cause oxidative damage. Glutathione is a master antioxidant that helps detoxify these ROS, preventing cellular damage that can affect a plant's pigment-producing mechanisms. Indirectly influencing plant colors By regulating cellular redox status and interacting with other molecules, glutathione also affects pigment expression in more indirect ways. The xanthophyll cycle: As part of a plant's antioxidant system, glutathione helps maintain the reduced state of other protective antioxidants like tocopherol and zeaxanthin. Zeaxanthin is a carotenoid pigment involved in the xanthophyll cycle, which helps dissipate excess light energy. Redox signaling: The balance between reduced glutathione (GSH) and oxidized glutathione (GSSG) is a key cellular signal for stress response. A shift in this ratio during environmental stress can influence the production of secondary metabolites like pigments, allowing the plant to adapt. " 9/19 1:41AM AI Overview The key difference is that anthocyanins are the sugar-containing form (glycosides) of pigments, while anthocyanidins are the sugar-free form (aglycones). Anthocyanidins are the foundational molecules, and when a sugar molecule attaches to them, they become anthocyanins, which are more stable and water-soluble, making them the forms found naturally in plants, such as berries and purple vegetables. Anthocyanidin (Aglycone) Structure: The basic, sugar-free molecule of the anthocyanin structure. Location: Not found freely in nature but is the core component that is then glycosylated. Properties: Color changes with pH, being visible in acidic conditions but colorless in basic conditions. Examples: Cyanidin, delphinidin, pelargonidin, peonidin, petunidin, and malvidin. Anthocyanin (Glycoside) Structure: Consists of an anthocyanidin linked to one or more sugar molecules. Location: Found in the vacuoles of plant cells. Properties: Water-soluble and are the pigments responsible for the red, purple, and blue colors in plants. Function: The sugar attached provides stability, allowing for accumulation in plants and providing antioxidant properties. Examples: Cyanidin-3-glucoside and other derivatives like acylated anthocyanins. " 9/19 2:43AM I also saw a good mans immediate accent into Heaven, that perspective matters too. no less angry about it though. 9/19 10AM Heavy Amino spray 250mg each in 1L of Setria Glutathione and Phenylalanine 9/19 10:10PM The sons and daughters of americas real terror organization carried out the last horror show, and the one before that and before that. It's not a foreign country, it's always the anti white anti human black sheets and badges that did this. 9/19 10:43PM AI Overview Phenylalanine and glutathione contribute to plant colors through different biochemical pathways . Phenylalanine is a precursor for the pigments themselves, primarily anthocyanins, while glutathione is involved in the transport and stabilization of these pigments within the plant cell. Phenylalanine: The pigment precursor The source of aromatic compounds: Phenylalanine is an aromatic amino acid and the starting compound for the phenylpropanoid pathway in plants. This pathway produces a vast number of secondary metabolites, including anthocyanins, which give many plants their red, purple, and blue colors. Color enhancement: Research has shown that increasing the amount of available phenylalanine can lead to more intense red coloration in some fruits, such as mangoes and apples. Pathway stimulation: Phenylalanine ammonia-lyase (PAL) is a key enzyme in this pathway that converts phenylalanine into precursors for anthocyanin biosynthesis. An increase in phenylalanine levels stimulates this entire process. Glutathione: The pigment transporter Anthocyanin transport: After anthocyanin pigments are synthesized in the cell's cytoplasm, they must be transported into the central vacuole for storage. Glutathione S-transferases (GSTs) are a family of enzymes that facilitate this process. Color intensity: A functional GST is essential for proper anthocyanin accumulation. Mutations in GST genes can result in a significant decrease in color intensity, as seen in the pale or white flowers of certain gentian mutants. Mechanism of action: The GST binds to the anthocyanin pigment and moves it from the cytoplasm into the vacuole. If the GST is defective, the pigment cannot be transported correctly, leading to a loss of coloration. Summary of interactions The roles of phenylalanine and glutathione are distinct but cooperative in producing plant colors: Phenylalanine provides the essential building blocks for creating the colored compounds (anthocyanins). Glutathione, with the help of GSTs, ensures these colored compounds are correctly moved to their storage location (the vacuole). This cooperative system explains why mutations or deficiencies in either process can lead to reduced or altered coloration in plants." 9/19 10:51PM AI Overview. Trimethylglycine (TMG) supports the production of glutathione, a vital antioxidant . However, there is no evidence to suggest a direct relationship between these supplements, the amino acid phenylalanine, and the colors of plants. The concepts are linked indirectly via complex biological processes. Trimethylglycine (TMG) and glutathione TMG and glutathione are connected through the body's methylation cycle. TMG as a methyl donor: TMG plays a critical role in the methylation process by donating a methyl group, which helps convert the harmful amino acid homocysteine back into methionine. Support for glutathione production: This methylation cycle, which is supported by TMG, is essential for producing glutathione. Glutathione is a powerful antioxidant that protects against cellular damage and is crucial for detoxification. Supplementation considerations: TMG supplements are sometimes taken alongside other supplements to support health, though consultation with a healthcare provider is recommended. Phenylalanine and the methylation cycle Phenylalanine is an essential amino acid, but its role is distinct from the TMG-glutathione process. Essential nutrient: Phenylalanine is a key nutrient for cellular metabolism. Potential interactions: A separate medical study on experimental hyperphenylalaninemia (abnormally high phenylalanine levels) in chicks observed a decrease in other amino acids in the brain, including those involved in the glutathione pathway. This demonstrates how excessive levels of one amino acid can potentially influence others, though this does not represent a typical interaction. Plant colors The connection between the supplements and plant colors is purely conceptual, as the colors are determined by completely different biological processes. Anthocyanins: The colors of many plants, including red, purple, and blue flowers, come from pigments called anthocyanins. Glutathione in plants: While plants contain glutathione as an antioxidant to combat stress, it influences color by regulating the transport and accumulation of anthocyanin pigments, not by being a pigment itself. TMG and phenylalanine in plants: Plants contain TMG, which functions as an osmoprotectant (protecting against osmotic stress). They also contain phenylalanine, but these substances do not directly determine the plant's visible color." 9/19 11:21PM. !!!!!! this was pointless and im dumber for having read it. !!! Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level: https://pmc.ncbi.nlm.nih.gov/articles/PMC6938384/ 9/20 11:08 AM the seedlings and the four autos are doing just great. The amino spray with phenylene and glutathione really had nice effects no burning nice solid growth even seedlings from basil lavender various lettuce all are perfect.🚨🚨🚨👍👍👍👍👍 9/21 2AM I AM BECOME ANTHOCYANID!!! ITS WORKING AND ON A SEEDLING I SEE THE GELATO COLLORS IN BLUEBERRY KO AND THE LEAF SHAPE OF BUBBLES BLUEBERRY,!!!

Ticking along ok the white widows are starting to thicken up I think another 2 weeks and they will be ready to go. GSC #1 is looking like it’s getting close just by looking at the pistils but I haven’t looked at the trichomes yet. The GSC#2 and #3 are just starting to fatten up and still producing a lot of pistils so I think they will be another 3 weeks.