I tied her down had to be real careful cause the stalk is so thick it’s crazy you cant really see much in the photos cause she’s so bushy she loves the nutrients been watering about every 3 days getting real close to flowering I think until next time happy growing

This is the whole flowering cyclus posted in one week.

Very explosive growth this week! Things progressing very well.

Finally got some videos up (I think)! One shows the canopy, the other gives an idea of the training under the screen. Shes finally showing signs of female and pistils are popping out like crazy. Decided to not tuck the final days of the stretch so the buds won’t start in the screen and start right above it, seems like I’d be easier come harvest time

Day 43. Girls will get heavy training later today. They grow ... and because of that i had to move 3 Kushes to other tent and i will make separate diary for theirs flowering time. 3 Auto Blue Tooth by Real Gorilla Seeds took theirs place. After todays training i will give them 4-5 days to recover and will send them to flower hopefully on sunday after 24 hours of darkness. Skunks grow bigger plants, than Cheeses, despite theirs quickest germination and obvious lead in first 3 weeks or so ... Day 46. 3 days left to flower, finally made heavy training, way too busy for LST grows... but result is good, girls hopefully will show off in bloom ! Day 47. Girls loved training, 2 more days, 24 hours darkness and tuesday latest flowering starts. Got full feed of Biobizz, feed charts updated. Next feed = water + silica. Happy Growing !!!

First week of flower in the books💯👊🏻

She fell over between transplant so its obvious she’s had some trauma. Will keep a close eye but I’m sure the will do just fine. Goal is to maintain temps and humidity at 80/60 this week. Was moved under 95W QB 3k

Buenas buenas!, cómo ven en esta 3ra semana de etapa de gestacion de esta critical esta bastante bien ( a mi parecer) , las veo crecer sanas y sin problemas, D.P.M. El transplante les ah sentado muy bien, las dejaré una semana más y luego el cambio... El alimento de la mano de la gente de advanced nutrients, eh comprado este kit q para mí está bien, ya les contaré y veremos los resultados juntos. De momento mis niñas están muy bien y promenten ser las verdaderas! 😎🔥 Buen fin de semana para tod@s, Buenos humos para tod@s.. 👍🏻

Max 3 Pflanzen

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Looks good, no problems... She´s doin fine 👍👍

Aug 9: Mimosa Shot is doing fine. She was slower starting than her wagon partner Sunday Punch EV, but she is liking the warmer weather. Got UV led lights from AC Infinity going today. These are designed for indoor so I can only put them out on nice weather days. Did this last year and seems to work. The UV lights make Edmonton in the fall feel more like California in the summer, or at least that’s the idea. UV is critical for trichome production so it’s a good way to boost THC percentage. Aug 14: rainy the last few days. Mimosa Shot likes to be a bit warmer. It was slower getting going in the spring, and now it seems to be suffering from a P deficiency. Reddish petioles, and a blueish tint to the leaves. She has received Power Bloom and barley just like the other plants, but still seemed to be lacking something. Ed Rosenthal’s Grower’s Handbook says P deficiency can be due to cold weather. Decided to try and dissolve 0.5 tsp of Power Bloom in 1 L of warm water to use as a high P foliar spray. As always, used a bit of Dr. Bronner’s Peppermint Soap as a surfactant, and apple cider vinegar to adjust pH. Power Bloom mostly dissolved but with some undissolved. Unfortunately I don’t have a working TDS or EC meter. Not sure how strong it was so just sprayed a bit on the fan leaves.

It wars too soon high lvl of Light, next time i need to chill with the 100%.

Almost ready to harvest, 7 weeks in flowering, lets see how many more days left. What do you think?

Growing nice so far. Still only fed with RO water . Next feed will be with 1-2ml of calmag, Touch of molasses to 4l water. Just to top up whats left in the soil. 600 w MH Tent temp is 21-23 degrees Celsius Humidity is 39-43% Ladies are loving it. 2 left plants are of peyote cookies