what can I say she is growing like a beast now! They plants are loving the weekly foliar sprays. The blood, bone and kelp meal are doing a great job. Will do another magnesium sulphate next week.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

This week I did a compost tea which they really liked. I just watered them with water. Ph 6.3. They’re still growing slowly but I think l figured out why. I had a light coming off of my extension chord that was in the tent. I didn’t realize it till last night & thank goodness I did.

Brand new here and my apologies as I'm actually on Week 5F at this time and have had to scour my journal, notes and photo's to catch this diary up to the present. Next run is 8 auto's( 2 FastBuds & 2 Seedsman) with 4 different strains so STAY TUNED! ...The babies are doing well with the exception that I had two Power Plant beans that failed to pop, third time was the charm which puts it roughly a week behind it's sista's. GSD is just hanging out desperately waiting for the little one's to catch up so she can flower as she's been vegging for 50 days already (I had her on a 19/4 light schedule with a Auto run that just finished). Temp's and RH good to go, daily light water right now with seaweed extract added @ 1tbs/gl.

Cuttings are growing very fast, we added 2 more lights for a total of 800 W led grow light. On day 11 since have been put in the box we decided to switch to flow as we tought cuttings has reached the right dimension to fullfil the box. Still using only pH 6.5 water with no nutrients

So basically for some reason I wasn't able to add my next week which caused me to start this "duplicate first grow diary". Then I decided trying to split my diary into four separate ones to better catalogue my grow, and I'm just having a lot of issues getting everything straight. SO! I decided to continue posting full scope pictures of the whole setup, all four plant sets included. Will try to update more on everything tomorrow!

This lady is getting better every week All of her sisters are too Amazing genetics with amazing nutrients I hit flower by mistake cuz she's still in veg

Aug 6th - Jack gets fed today; 3 gallons first meal w/all bloom nutes. Aug 7th - Whats happened is I have taken 2 of the smaller Colas from the back of the plant, and brought them Forward and out the front, low. They are official branches but they were really limited in that location. So down in front they, or it may even look a little lollipopped - Foliar feed tonight @ sundown - Kelp Meal worked into the Compost at the surface - Pistils are present on all branches, its Flower 🌸 time Aug 8 - 4 branches took off the last couple days, and they were levelled for the last time Aug 9 - plant looks great today, pic attached - Foliar spray tomorrow if possible. Then only 2 more next week finishes the fulvic acid / epsom salt addition through the leaves before proper flowering begins week 3 - next meal posted above; w/ Molasses (tomorrow) BUT, rain is forecast for the next 2 days, so the meal can wait. I have put some fresh compost on the base of the plant… 10th - massaging a few of the branches, and what a smell of Lemon - evening, heading right into Flower, I defoliated lower Nodes up to 29” and any obvious shadow makers - super-cropped a couple smaller branches at the half-way mark on the cage. Front & Center. Now they will participate 11th - looks strong - last 2 cups of liquid phish poop added to the compost 12th - solid rain overnite, more predicted. Looking at Sun/Mon feeding (15th/16th) - last Foliar Spray this morning b4 the heat or rain kicks in… and b4 too far into flower. Foliar spray is now done.

Day 35, start of week 2 in flower, everything is going great so far✌️🏼🌱 Day 37 - All the ladies are looking great✌️🏼🌱 Day 38 - ladies got watered today, looking good & starting to put off a nice smell😍 Day 40 - Still have a lot of yellowing leaves towards the bottom of almost all the plants can’t figure out exactly what’s wrong😅😅

I completely changed the water and nutrients this week and added some Canna PK13/14. I have also added another MARS Hydro TW2000 light to the tent to try and get more light to the lower buds. Gave both plants a trim also for the same reason. I was concerned they were still not ready to harvest, but expert advice from FastBuds420 told me they probably needed a further 4 weeks. And I will give them an update and the information they requested. I think it's probably because I had the lights turned down to 25% during the veg stage and only at 50% for the early flowering stage; it was not till recently, about 3 weeks, I think the lights got turned up to 100%.

Flushed all the plants today. I water every 3rd day, so maybe two more flushings before harvest.

This has by far been the most stressful part of the grow. as I'm trying to increase yield as much as possible, and in doing so f£&ked around and found out the hard way. She has gone into what I would say is severe shock this on the 31/1/25 will be the 3rd day of shock hopefully she can pull through and start growing again 🙏 🤞

Week begins 8/12 LAST FEED 2 gallons 8/12 AFTER A RAINING NIGHT. 8/13 I've found what appears to be septoria on a couple plants. Looks like the weather will be OK tonight so I can apply organocide or something similar before dark. I've seen random pillar damage so this will be a two-for. I also should move the middle plant back and utilize my space more. Ph seems on point around 7.3. Higher in some places of the soil. I've noticed what looks like root issues on my blueberry. Winds were horrible yesterday and this plant doesnt hace a vertical trellis for support. Stem was pretty wobbly for a while but the plant seems to be growing fine. Either that or a jpn beetle munched the top of a bud. That's probably what happened. It was in the low 50's this morning. That's a 40 degree temperature swing in just a few days. This grow season has been the most challenging I've faced. Of course it had to happen when personal, family, and health concerns rear their head. Oh well. I can only do my best. 8/14 Went over at 5am to apply organocide to treat WPM or septoria as well as insecticide for pillars. I got there and one of my healthiest plants had been turning a little lime green now it was drooped over just like the larger GDP. This one is a 9lb kush though and they were doing good. Now it looks like it's dead over night. Maybe it's that liquid kool bloom. The soil seemed dry so I watered the plants. It MIGHT have picked ok a little. I'll have to check tonight. If not something is going on at night or in the rootzone and I really don't know what the fuck is going on. I will post updates and check later tonight. Damn. This has been a real challenging year. 8/15 That plant might be overwatered or not be draining correctly. At least I was able to get underneath it and the ground was dry. It was also hard packed dirt so elevating the bag may help. I'm going to wait a few days and see if things improve. This really sucks. I'm getting that defeated feeling. I need to get out of it though as I'm starting to see flower begin. The blueberry is probably the farthest along. I need to remember to take pictures of the newly developing flowers. Granted I've had numerous medical and other issues that have taken me off my game but losing two plants in one year is pretty shitty. I was able to fix things with COVID last year or the year before so I hope I can at least save this 9lb kush plant. If I lose this plant too it will be a substantial amount of cannabis lost. I need to rearrange my cage as well to make use of the extra space. Maybe I could've overwatered that plant that had been in the rain for a long time. I need to get my water routine down pat instead of doing the "lift the pot" method I have been doing. I went to several different commercial growers and got a bunch of crazy answers. Only time will tell. Last night I finished the organocide application on all the other plants except for the two babies and the drooped 9lb kush. Don't want to stress it further. I'll see how it goes. 8/16 Despite the soil drying out the newest disaster (my 9lb kush) is much worse with chlorosis killing leaves. Stalks and branches seem good. Soil smells musty and a little wetter than the rest. It was roofed to the ground slightly. NO water underneath. I stuck my hand in the bag from a bunch of angles. It's like the same thing that happened with the other one. I added to my surveillance. I also smell bleech and I hace an idea this might not be my doing at all. I can't be detailed but I'll find out one way or another. UPDATE. Went back over after the dentist and plants were dry as a fucking bone and droopy. I mixed up five gallons of phed water and watered everything. Even the 9lb kush that is pretty much dead. The soil was dry though. Everyone is saying overwater but I really doubt that. We had some massive rainstorms bur still. I need to review a game cam I have because unfortunately my cams went on the frits for a couple days (and the dogs went wild during that time around midnight) couple nights in a row. I fucking smell bleech. I'm a good dude and don't really have any enemies but we'll see what the game cam pulls up. 8/17 Of course we are getting a shit ton of rain. I watered yestetday because everything was dry as a bone I watched yesterday as my blueberry was almost horizontal due to the wind. I added several supports to every branch and a few more up the main stalk. That may be what has been happening. The wind blowing so hard it separates the stalk from the roots. My problems started after I took my windbreaker tarp down. I put it back up for this storm. Or maybe it's just root rot. I dont fucking know. I cut all the dead stuff off the 9lb kush thats pretty mucb dead. Its holding on though. I probably shouldnt have watered that but it seemed bone dry. Maybe further down was wet. I dont know. Extra cams are up. I didn't smell that chemical bleachy smell today so it's probably in my head. I have better peace of mind with the night cams up though.and the dogs though. I've had a visitor this year but he left very quickly. 8/18 We got a shit ton of rain last night. No damage though. I spent what time I had in the garden working on the 9lb kush that looked like it was dieing. I'm not giving up on this one so easy. It seems to want to hold on. Despite the severely overwatered medium (due to this storm) I cam see new growth that looks healthy. The branches also seem stronger. I'm cautiously optimistic. The other one straight up died. So I might be able to salvage this. I'll go over later today and check on it. I debated putting it inside for the storm bur I figured I'd let the rain leech out anything in the medium in case it's locked out or something. At least I've got a couple real healthy ones this year. That NYCSA is amazing. That's a strain I'll probably grow again. Resilient as all all. Vigorous and doesn't seem impacted by things like WPM or other fungal ok infections or diseases like some other strains. I got a great recommendation from my buddy to use pots all the same size. It makes things easier watering. I just used what I had this year. I had bigger pots so I wanted to fill them. That tarp being up I think greatly helped with the wind. It's down now so things cam dry out. Still raining bit not yard and no wind. I think that may have played a large role in killing the 7ft GDP. Wind ripping the stalk away from the roots. Or it could've been a million other things. I'll never know. I do know that I have a better mindset, a great set of resources and I'm learning something new every year. If I can br8ng this plant back to life I'm going to be very proud of myself.....and her. Oh and I'm going to buy new tiger bloom. I'll start beasty bloom (hesitant as it has caused lockout in the past) as it goes better with my other nutes than the liquid kool bloom. Plus I started getting portables around tge time I used the liquid kool bloom. That being said, after two weeks I can see buds where there weren't before so I don't know. Next year I'm using seeds and I'm doing things different. I'm STRONGLY considering rapacaps idea about adding a removable roof for flower. The more I think about it tge better it sounds. That and putting wheels on pallets (buddy suggestion) and I could attach my supports directly to the pallet. I've got a lot of work to do that's for sure. Plants look healthy but I needvto him them again with organocide to fight any fungal stuff or pillars that might be hiding.

Wow she's frosty!!!??!?!! Did I mention that before? 😁 😂 Trichomes going cloudy now.

Welcome to week 4 of my Powerplant XL by Zambeza Seeds Disaster happened last week, a bunch of thrips attacked the plant, and the weather was very hard, so near to now growth progression. Just waiting for recovery, let's keep our fingers crossed.

Day 172 21/10/24 Monday I had 1.5 Litres of feed left, and she was top dry this morning so she finished that off today. Picture update 😎💚 I'll get one later with lights off 🙌 Day 174 23/10/24 Wednesday De-chlorinated tap water pH 6 only today. Finally update pictures and videos 📸 💚 She is developing awsome colours, and buds are now starting to stack weight 💪🙌 Day 177 26/10/24 Saturday De-chlorinated tap water pH 6 only today, noticed a few tips burning. She is stacking !! This week she has developed a really floral aroma is sweet and tantalising like a perfume almost. She is glistening with trichomes, and buds are taking the dark 🌑 colour with purple/pink calyx tips. Super duper sticky!!

Well she looks a bit better than last week!! Still chugging along. 6.5cm vertical growth this week.

These buds are absolutely awesome, very frosty solid dense nugs👍 have been flushing this week as there ready to harvest . Orange pistills cloudy trichomes with some amber's & the clusters have closed up . Will give them 48 hours of darkness before harvest.

Day 106. Plants are okeish , A got last watering, think Saturday is last day for her. Will try to push plant C for 7-8 days after. Plant B looked almost revenging, didn't liked standing in the shade for sure ... Will feed him at least twice. Happy Growing !!!