Not particularly growing for speed or any dedicated reason this time round, just adding water and a abundande of positivity, gotta admit I do like the autoseed logo. Off we go again into the magical forest. (Arthrospira platensis) cultivated in high-alkaline, mineral-rich water in Kailua-Kona, Hawaii. It is renowned for having a higher nutrient content, including more carotenoids and essential fats, compared to other spirulina brands. It is frequently cited as a "complete food" due to its dense concentration of proteins, vitamins, minerals, and pigments. The nutritional profile of standard dried Spirulina platensis biomass. 3kg of spirulina powder has an approximate NPK ratio of 10-2-1 (or 10% Nitrogen, 2% Phosphorus, and 1% Potassium). Here is the breakdown of the nutrient content for 3kg of powder: Nitrogen (N): ~300g (10% of total mass) Phosphorus (P): ~60g (roughly 2% of total mass) Potassium (K): ~30g (roughly 1% of total mass) 3kg of spirulina powder is more than enough nitrogen for 4 cannabis plants in a 100-gallon pot—in fact, it is likely excessive and could cause severe nutrient burn or toxicity if not properly managed. Spirulina has a very high protein content (46% to 63% dry weight), which translates to a significant nitrogen source. It is often described as a 5-1-1 NPK fertilizer. 3kg (3000g) of powder in 100 gallons means 30g per gallon. General organic recommendations for potent amendments (like frass) are often around 1–3 tablespoons per gallon. 30g of powder is roughly 2-3 tablespoons, making this a very high-strength application rate if added all at once. Balance this with calcium/magnesium, and organic nutrient delivery in soil growing can lead to deficiencies in these nutrients. Not all of that 100 gallon is used, so figuring out precise measurements was ballpark, but there is about 10kg of engineered biochar in that pot. Mostly in the top half. Ideally, I'd have 25kg in a full active 100-gallon, but......... don't mess with something that is not broken. Roots do not utilize the entire 100 gallons. This has been a couple of years of trial and error, mostly error. The amount of cations you can add in grams depends entirely on the specific cation exchange capacity (CEC) of your biochar and the type of cation being added. Biochar CEC values vary widely, typically ranging from 4 to over 300cmolc/kg. Multiple Biochars in use, ranging upwards of 100cmolc/km. Different cations have different atomic weights Potassium K+ 39.1g/mol. Calcium Ca2+ 40.1g/mol. Magnesium Mg2+ 24.3g/mol. Nitrogen NH4+ 18.04g/mol. You get more nitrogen bang for your buck. It all depends on the CEC of the biochar you use, but brrrrrrrrrr roughly I'm looking at 500-600 grams worth of elemental cation exchange capacity, give or take. That's just for the biochar alone. Elemental cation exchange capacity refers to the ability of materials—such as soil, minerals, and biochar to adsorb, hold, and release positively charged ions Ca2+, Mg2+, K+, Na+, Al3+, and Zn2+. This capacity is a critical measure of nutrient availability in soil and energy density in electrochemical systems. Added calmag 2:1 to complement spirulina. Calcium (Ca): Approximately 150g - 300g of elemental calcium. Magnesium (Mg): Approximately 75g - 150g of elemental magnesium. So long as pH stays in 6.3 to 6.7 ish, the plant will unlock hydrogen. Plants will be able to cycle CEC for their own needs. Hydrogen ion pumping (proton pumping) is a fundamental mechanism used by plant roots to facilitate the cycling of nutrients within a medium's Cation Exchange Capacity (CEC), directly increasing nutrient availability. A growing medium's Cation Exchange Capacity (CEC) does not contribute directly to Electrical Conductivity (EC). CEC represents potential nutrient storage, while EC represents active nutrients in the water/soil solution. A medium with a high CEC can hold many nutrients, but if there is no water present, the EC will be low. 98% of plant nutrient uptake comes directly from the soil solution. 2% of nutrient uptake is directly from soil particles. Nutrients held on exchange sites (CEC) are not directly measured by EC until they are released into the soil solution through exchange with other ions (e.g., hydrogen ions from roots or fertilizer). Plants source the H+ ions (protons) used in cation exchange primarily by exuding carbon sugars into the rhizosphere, then microorganisms perform root respiration, which reacts with soil water to form carbonic acid H2CO3 that dissociates into H+ and bicarbonate. Roots also actively pump out H+ ions directly to acidify the rhizosphere, plants trade hydrogen produced through metabolism for essential nutrients held by the soil. In plant cells, the H+ (protons) used in proton pumping across the plasma membrane come primarily from the cytoplasm (cytosol). The protons are derived from internal cellular sources, including metabolic processes that release H+, and the regulation of intracellular pH homeostasis. Distilled water is highly useful in managing the electrical conductivity (EC) of a growing medium by allowing for precise control over nutrient concentration and assisting in the mobilization of nutrients from the cation exchange capacity (CEC) into the active, plant-available nutrient solution. Just add water & positive energy. Potential hydrogen. pH. + - , potential charge balance for hydrogen. So long as there is adequate oxygen in the soil and air and water moves at a decent interval, its practically impossible for the pH to skew in a soil that is taking in oxygen and releasing CO2 and nitrogen, I say practically, but meh, even with plenty of oxygen, the process of converting ammonium NH4+ to nitrate NO3- by bacteria releases hydrogen ions H+, which directly causes acidification (lowering pH). While water movement is necessary, consistent water moving through the soil can leach away basic cations Ca2+, Mg2+, K+, Na+, leaving behind acidic hydrogen and aluminum ions, causing pH to drop, especially in humid regions. High levels of CO2 in the soil (from intense respiration) dissolve in soil water to create carbonic acid, H2CO3, which lowers the pH. (barometric pumping removes that possibility) The pH in a cannabis cultivation environment, particularly in hydroponic or soilless setups, will often naturally drift towards acidic (lower pH) over time. Understand how to know when to lime the pH back up. Complex but critical. Once I understood this it helped ease the pain of understanding pH. Base saturation directly reflects the percentage of CEC occupied by base cations. Generally, as base saturation increases, soil pH increases because fewer acidic cations, H+ and Al3,+ are present on the soil colloid surfaces. At a pH of 7 or higher, the soil colloids are saturated with base cations, resulting in a base saturation of 90+%. While many sources include Sodium Na+ as a base cation, some calculations focused specifically on plant nutrients (like in some nutrient management contexts) may focus solely on the "Big 3": Ca2+, Mg2+, and K+. However, in soil science and CEC (Cation Exchange Capacity) measurements, sodium is usually included in the definition of total base cations, making it the "Big 4" base cations. At a pH around 5.5, soils often have a 45–55% base saturation, while a soil with a pH between 6.5 and 6.8 generally has an 80–90% base saturation. Base saturation is considered a reliable, albeit complex, indicator of soil fertility and the degree of soil weathering, acting as a crucial metric in soil classification and management. Once you take full control of the pH, so long as there is oxygen, and one keeps tabs on base saturation levels. Its near impossible for anything to go wrong. All you are essentially doing is adding water and waiting for any sign of deficiency. Not so much growing as eliminating the possibilities of things going sideways. Can't over or under water, can't skew pH, can't go anaerobic. Everything runs in optimal cycles, watering every 2 to 3 days or as required, depending on the rate of combined ET. A little warmer, a little drier, but never short on water. All the nutrients are already there in the medium, we still focus on the water, just not adding salts, but to mobilize nutrients held in the Cation Exchange Capacity (CEC) and move them into the active soil solution for plant uptake, the best supplements to add to water are fulvic acid, humic acid, or seaweed/kelp extract. Liquid solutions with high concentrations of essential trace elements. Distilled water has no alkalinity or minerals to help buffer or release nutrients. Adding these supplements turns the "empty" water into an active agent that forces nutrients off the CEC colloids into the solution for plant uptake. When to water? When Electrical Conductivity of soil gets low. No water, no conductivity.

Day 58 - Adding Big Bud Coco to the nute mix, seems that it's doing its job 😁 (Yes, I need a bigger closet 😅) Keep on growing 🙏😋 Day 63 - Haf some problems with my EC pen the last days, it wasn't clean so I overfeed a little bit but I noticed almost immediately so I've cleaned it and re-done the nute solution. Just a few tips of Gelato's leaves were burnt, nothing to worry about. I think 4 weeks from now and the Purple Lemonade will be ready for harvest, so other 5 days of Big Bud and then I'll switch to Overdrive for the final boost before ripen and flush 🙏

Good yield, strong tasty weed

Vienen creciendo muy bien. Se nota la diferencia de tamaño entre las que están en 30L y las que están en 50(tienen la tierra por la mitad en maceta de 100. Espero en unos días poder transplantarlas también

Hello everyone week 5 of flower has passed for this Vanilla Latte auto 🍼 For the feeding schedule i stopped feeding Power Roots and Pure Zym and started feeding Green Sensation 0,5/l Mars hydro FC-E6500 75% have a great day and wish you all happy growing 😎👨‍🌾🏻

Trichomes are milky but the pistils have too many white ones still. Flowers have slowed down in their growth. Another week to go maybe.

Starting to ease up on the nutrients with week 7 coming to an end. Starting week 8 plants will only be fed ph water and molasses until plants finish.

Day 35: This week I was busy again with work (60hours this week) + Christmass days with family + friends(4days every evening). So you can gues, i asked my friend again to take care of them. Well he did kind off, but he forgot to adjust the lights and dance the plants arround(shuffle them, sorting by height). What happend? They stretched out because of the heat and he gave them to much water everyday, he tried to do his best this time but he did the imposible, he overwaterd cocos soil. As you can see the leafs dropped downward indicating overwatering or pests. In my case, luckly, the first one. Luckly easy to fix. Also spotted some stress in stems due stretching, something i cannot fix. Look at week 4, pictures of day 28, thats my work after I fixed it for days and now look at this week.... For f**k sake lol. Because of this stretching it looks really crowded now instead of a beautifull equal cantopy. if you look at picture one to the plants in the right corner, those are the heights I want them to be. There are still some plants that fell behind and barely recoverd since last time. For the new week I will have to make a choice to eighter keep them and hope they still grow some while flowering or I will need to get rid of them in exchange the others will have more space. I might thoose the last option. Thats it for this week, see y'all in the next.

Weekly update. Sorry it was a late update this week I was out of town (Bizcon2024). Wow have they grown, I'd say doubling in size. I've upped the watering to 1000ml daily, this week I'll do a major defoliation and lollipoping. All in all Happy Growing

Flipped to flowering on these girls as the screens are now almost full across the 4x8 tent and we will still need to train them along the screens for the next 2-3 weeks. So working for a packed tent this time hopefully. Plants have been exploding in growth daily. Water was changed out and new water added with 3/4 Remo Flowering lineup and UC Roots at full strength. Smell is starting to get noticeable when you open the tent and is very pleasant.

I’m very impressed with my first clone attempt doing good so far I will have to start some type of training not sure which way to go I will appreciate any Suggestions thanks

Flowering nicely now. Not drinking a ton of water but I attribute that to lower temps lately.

She responding to nutrients nicely.

N1 is big and super frosty already. N2 much slower

Only running 1 Thin Mint x Cookies + Cream its front left in the tent, great internodal spacing came highly recommended. Switching to Bloom nutrients, two part Cropsalt. Irrigation frequency will be adjusted as flower development drastically increases over the next two weeks.

Final week at week 11 of flower. I think I could have let her go a little Longer but she’s probably around 10-12% amber. Looking and smelling great!! Chopped, hung, dried, trimmed and cured. Bud tastes great and is potent. Not going to be a couchlock for the everyday stoner but definitely a great strong sativa type high. Packed on a lot of weight and was happy with the outcome.

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""