2021 - 08 - 29 Wedding Glue Auto 1 - most fan leaf are starting to turn yellow , Hoping she finished up in a week to 2. - Day 55 since sprout - trichomes are mostly milky, not much amber on buds yet. Wedding Glue Auto 2 - Some of the buds and fan leaf with abit of sugar leaf are turning purple... - Day 50 since sprout - She's fattening up nicely image format : Wedding Glue Auto 1-4.1 day 27 Wedding Glue Auto 1( plant strain and tag number) , 4 (week), 1 (image number) , day 27 ( Date since sprout )

These girls are doing great.they are very healthy and growing vigorously,there are two distinct pheno types.the taller have alot more trics and they are about a foot taller.the small ones barely have any right now and are about 18 inches,I'm sure that will change pretty quick though.there isnt very much of a smell yet,probably a 2 out of ten right now.

Week 16 (16-6 t/m 22-6) 16-6 Temperatuur: 31 graden (licht aan) 23.4 graden (licht uit) Luchtvochtigheid: 50% (hoogste) 35% (laagste) De toppen zien er echt super goed uit, ze worden steeds wat vetter en ze zijn echt goed compact. Het ruikt ook echt heerlijk, als ik de top aanraak dan ruikt het echt super lekker zoet en mijn vingers plakken gelijk! Ik krijg gewoon water in mijn mond hahaha. Deze week geef ik haar al wat minder voeding, vanaf volgende week alleen afgezuurd water voor de flush. Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium en 0.25 gr PK+ PowderFeeding per liter. EC: 0.8 PH: 6.4 17-6 Temperatuur: 31.5 graden (licht aan) 23.8 graden (licht uit) Luchtvochtigheid: 52% (hoogste) 40% (laagste) Vandaag de lamp op 100% sterkte gezet. Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium PowderFeeding per liter. EC: 0.5 PH: 6.4 18-6 Temperatuur: 32.9 graden (licht aan) 23.8 graden (licht uit) Luchtvochtigheid: 62% (hoogste) 40% (laagste) Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium, 0.4 gr Hybrids en 0.5 gr PK+ PowderFeeding per liter. EC: 1.6 PH: 6.4 19-6 Temperatuur: 32.8 graden (licht aan) 24.5 graden (licht uit) Luchtvochtigheid: 59% (hoogste) 42% (laagste) Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium PowderFeeding per liter. EC: 0.6 PH: 6.5 20-6 Temperatuur: 32.4 graden (licht aan) 24.9 graden (licht uit) Luchtvochtigheid: 66% (hoogste) 42% (laagste) Ik weet het niet zeker maar ik denk dat ik een stuifmeel zakje zag en heb dat voor de zekerheid maar weggehaald. Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium en 0.25 gr PK+ PowderFeeding per liter. EC: 0.8 PH: 6.4 21-6 Temperatuur: 32.9 graden (licht aan) 24.7 graden (licht uit) Luchtvochtigheid: 71% (hoogste) 53% (laagste) Vandaag nog een stuifmeel zakje weggehaald. Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium PowderFeeding per liter. EC: 0.6 PH: 6.4 22-6 Temperatuur: 32 graden (licht aan) 23.1 graden (licht uit) Luchtvochtigheid: 76% (hoogste) 51% (laagste) Volgens het schema moet ze nu nog 1 weekje en dan kan ze geoogst worden, ik ben benieuwd! Watergift: 1100 ml, met 0.2 ml Silica Power per liter, 0.3 ml CalMag per liter en 0.8 gr Calcium en 0.25 gr PK+ PowderFeeding per liter. EC: 0.8 PH: 6.4

I had to top Cantaloupe haze and White widow as they were too close to the lights and I didn't want to loose any ppfd on my lowers. My GSC seems perfect for not topping. next time I would top those strains at least once. I have been flushing the CH and getting run off around 1200ppm which seems like the max she can take the others like it stronger so I have been flushing them every 2-3 days. CH is tough to keep happy but will continue to monitor. All plants are in week 4 of flower 12/12 from seed.

We are at 21 days from these girls breaking the surface and they are making progress. I noticed at the beginning of the week that they were a little overdosed on nitrogen so I added 2 gallons of tap water to the reservoir and adjusted the ph to 6. They are still looking a little heavy on N but their growth rate is going great so I think it should balance out by the end of next week. I dont plan on doing a water change until I flower them. I left the plants alone most of the week. Tied most of them down to spread them out and defoliated a few lower leaves on day 20. The branches on most of these plants are thick and solid already. They don't budge easily. Bananasicle is looking pretty funky with uneven branch and node growth but we are just going to push forward. Still pumping nutrients and beaming light 24 hours a day. I'm thinking day 28 we will switch them to flower and see if any of these ladies can bring some❄️🔥💨

We harvested one little bud this week. Now we have realised that it was probably premature, but the pistils were orange so we just decided to. We are drying it outdoors in a plastic box with a fan attached to a little solar panel. We will harvest more next week, but right now it is not looking good in terms of the harvest.

Day 14 After a week from the bottom feed The pots feels light again My finger was dry So i think its time for they first halve dose feed 23c, Ppm 700, ph 6,25 Gave each a dose of 100ml water Day 16 Last night I saw roots so today I transplanted Wich didn't really went as plant because something (Hopefully only soil) dropped right before I placed "Jane" into her new home :( Besides that it went okay Gave them a good dose of water Now wait till it dries and then they get their first full feed dose Day 20 This night they got they first full feed 500ml each 4ml grow A&B 4ml b52, bud candy and voodoo With a low ph to bring down the ph in soil Wich is a lil high I qeuss 22,7c 1000ppm 5,8 ph Day 21 Today we did some LST Now they lay, next up is sit and then we roll So far they are cooperating Day 22 All are now bend and secured and doing well :D Day 23 Soil was dry gave them some calmag 1ml/l each 700ml 6.10ph

Pics are from 1/18-1/24 Girls are still behaving really well!! My “fan leaf” OCD has been kept in check, and am proud of myself!! The only other annoying thing, is that, one plant is almost twice as long, as the other 3!!

flower week 8, 2nd week of flush and my 15th week of crappy sleep due to this VIVOSUN 4"high cfm fan. I would just get rid of it as it does not owe me anything (2 full grows) but I am planning on gifting my old complete set up to someone I know that's in need. and shit.... Spider Farmer never even got back to me....so my next grow will include my first HLG to supplement the Spider.... no contest this month due to...... well I gotta move. I don't think id be winning any contest but on the off chance I would.... address change. lol HAPPY FRIDAY

This plant has a solid stench of pure ripe lemons. So much aroma and color in every bud, purples, to dark green, to lime green, to solid frost. This one definitely a fun strain to watch grow and tend too for the last few months. She’s definitely a hungry girl, seemed to be the only strain out of the three that accepted and thrived with full feed nutrients. Would definitely recommend this Strain to someone For a nice uplifting day smoke, also seems to do me good for some pain and stress relief.

Okay growmies here we are at the end of our PURPLE LEMONADE by FAST BUDS. It was such a pleasure to grow, i learned so so so much this run that will help me through the rest of my adventures. The beginning was tough as i am new i over watered and did not ph the water and what i mistake. But its okay i learned. She bounced back like nothing and produced these HUGE, THICK, DENSE BUDS!i remember the panic i had week 2-3 and wanted to scrap all of this and start over.... thank the weed gods i didnt because look at this masterpiece. I always said in the passed weeks comments i hope my ugly duckling becomes a beautiful queen. And here she is. She was SOOO HUNGRY AND THIRSTY! Over 5L a day guys..... and not a SINGLE drop would come out of the bottom.... over a all a pleasure and amazing grow. I will for sure grow again and again. I would love to Try this out door or in a 10L pot can probably get this beast to be the size of a 5x5 easily! She responded so well to LST and topping... slowed it down but it was so worth it guys lmao.... like honestly OVER HALF A POUND!!!!!! GROWMIES! I LOVE LOVE LOVE YOU ALL SO SO MUCH! Thank you for all the continued support and likes and comments and help. Id be no where with this with out all of you. And thank you to the FAST BUDS team and to you heather! Grow diaries thank you for the plat form. I love you guys. All of you. All my sponsors. Thank you. Until Next time growmies! Stay positive, stay safe, stay high.

The pictures speak for themselves. All is going fine and fastly. If this continues by the end of August this fantastic bush will be ready to be crop. Resin production is started. Next week bloom nutes will be added.

Day 78🌱🙂🍊 This lady is doing her great job, Buda are increasing rapidly 🌱 difference between top flower and bottom is evident. For this reason I’m quite sure I’m going to cut in two times, around 85/87 days I’ll cut the high part of biggest bud and around day 95 the rest of the plant. Day 80🌱🌱🌱🙂🍊 I’m going to go up to 90/95 days, smell now is really strong 👃🌱🤣 I keep giving some water when is really dry because some parts of the plant still needs it. Day 81 🌱🌱🌱🌱🙂🍊 Hi all fellows, today a big defoliation cleaning to improve light in the bottom Part of the plant before harvest. I tought to harvest in like 10 days but as I have some water yesterday I noticed that she’s been quite not drinking anymore. I’ll give another 2/3 days before harvest. I will be perfectly in 85 days from seeding. Smell is crazy now, I can smell cotton, orange, chocolate, pinus.. can’t wait to try it. Let’s see! ANY SUGGESTION IS REALLY WELCOME 🌱🌱🌱🌱🌱🌱🌱🏆🏆🏆🏆😊😊😊😊

Se levanto de pana , si alguien me puede dar consejos de como regar mejor , se agradece ajshsj Pd: El video es un tomate pera con lst

7/28 1220AM MONDAY.... Bubbles are looking ok, I need to trim them back to 4 colas, I am really stressing them with small space limitations, and temps at 80 to 81 7/29 Tuesday 5:45 AM💦💦 6:15 AM water with methylene blue two pills of nitric boost amino acid, five drops of Quick Sorb and I added bud factor X today and a quarter mL per liter of rhino silica💦💦💦. 1030 AM I don’t know what I’m gonna do. It turns out the bubbles plants are terrible. The stems are all very weak and they’re growing bushy at the top. I didn’t get them separated so all of the side branches grew up really long it’s completely useless. It is absolute this plant. It will be thrown in the garbage. Can you fucking believe I have to throw another entire plant in the fucking garbage😡😡😡😡. TREVOR plant cut down to 2 colas only, very top heavy not liking my grow space, had 2 more than I wanted so it's all fucked.... will remove shitty small colas and get maybe 2 good colas per plant, not expecting much. 1219pm....HOLY SHIT I FUCKED THESE.....TOOK OFF SOME COLAS.....NOT ENOUGH SPACE AND I PUSHED THEM TOO FAR.....I CAN FIX IT 7/30 1215am found a GREAT science link::: ""https://www.frontiersin.org/research-topics/8125/cannabis-genomics-breeding-and-production/magazine"". 345am PROLINE WILL BE HERE TODAY.ALL RESEARCH INDICATES PROLINE WILL PROTECT FROM HEAT STRESS!!!! 11:30 AM they are good again they are recovering from heavy defoliation half of the plant was taken off of one of them. I pushed it a little far but today they are recovering from heat and lack of air and lack of space the leaves are reaching up and they are not folding under heat stress. They look good today. Fast recovery heavy amino yesterday.👍👍👍👍🚨🚨🚨🚨 🚨🚨🚨🚨🚨🚨🚨🚨 GOT THE AMINOS AND HERE IS THE GROK RESULT OF MY MIX.......🚨🚨🚨🚨🚨🚨🚨🚨 The effects of applying essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine to cannabis plants can be significant, as these compounds act as biostimulants, influencing growth, stress resilience, and secondary metabolite production. The available research, primarily from studies on amino acids in plants, including cannabis, provides insights into their roles, though specific data on this exact combination is limited. Below, I summarize the effects of each compound on cannabis plants, drawing from relevant studies, and infer potential synergistic effects based on their known roles in plant physiology. Note that TMG (betaine) has less direct research in cannabis, so some effects are extrapolated from other plants. ### 1. Essential Amino Acids (EAAs) EAAs (e.g., leucine, lysine, methionine, etc.) are critical building blocks for proteins and play roles in plant metabolism and stress response. In cannabis: - **Growth and Development**: EAAs enhance protein synthesis, supporting shoot and root growth. Foliar or soil application of EAAs (e.g., 150–300 mg/L) increases leaf chlorophyll, shoot biomass, and root development in various plants, likely applicable to cannabis.[](https://www.nature.com/articles/s41598-022-21273-6) - **Nutrient Uptake**: EAAs like methionine and lysine improve nutrient absorption (e.g., nitrogen, iron) by acting as chelators, potentially enhancing cannabis nutrient efficiency in hydroponic or soil systems.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) - **Stress Mitigation**: EAAs, particularly methionine, support antioxidant production (e.g., glutathione), reducing oxidative stress from drought, salinity, or high light, common in cannabis cultivation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.00928/full)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cannabinoid/Terpene Production**: Limited evidence suggests EAAs may influence secondary metabolism. For example, methionine could enhance sulfur-containing compounds, potentially affecting terpene profiles in cannabis.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) **Application Notes**: EAAs are often applied via foliar spray (150–300 mg/L) or soil drench. Overuse may lead to nitrogen imbalance, so moderation is key.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### 2. Proline Proline is a non-essential amino acid that accumulates in plants under stress and serves multiple functions: - **Stress Tolerance**: Proline acts as an osmolyte, stabilizing cell membranes and scavenging reactive oxygen species (ROS) during drought, salinity, or temperature stress. In cannabis, exogenous proline (e.g., 50–100 mM) may enhance tolerance to environmental stressors, improving resilience in outdoor or controlled environments.[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW)[](https://pmc.ncbi.nlm.nih.gov/articles/PMC3548871/) - **Limited Growth Effect**: Proline alone is a poor nitrogen source for growth. Studies on Arabidopsis show it has a neutral or inhibitory effect on growth when used as the sole nitrogen source, suggesting it’s better for stress response than biomass accumulation.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Cannabis-Specific**: Proline helps cannabis maintain water balance, potentially improving resistance to extreme temperatures, which is critical for outdoor grows.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) **Application Notes**: Foliar or root application (50–100 mg/L) is effective for stress mitigation. High doses may inhibit growth if not balanced with other nutrients.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full)[](https://www.researchgate.net/publication/349966775_THE_ROLE_AND_IMPORTANCE_OF_AMINO_ACIDS_WITHIN_PLANTS_A_REVIEW) ### 3. Trimethylglycine (TMG, Betaine) TMG is less studied in cannabis but known as an osmoprotectant and methyl donor in other plants: - **Stress Protection**: TMG stabilizes proteins and membranes under salinity, drought, or temperature stress, similar to proline. It may protect cannabis plants in harsh conditions, maintaining photosynthetic efficiency.[](https://www.mdpi.com/2311-7524/9/7/807) - **Methylation Support**: TMG donates methyl groups, potentially enhancing secondary metabolite synthesis (e.g., cannabinoids, terpenes), though direct evidence in cannabis is lacking. Its role in methylation could support metabolic pathways linked to EAAs and glutamine. - **Growth Effects**: Limited data suggest TMG may improve chlorophyll content and photosynthesis, indirectly supporting biomass in cannabis. **Application Notes**: TMG is typically applied at 0.5–2 g/L via foliar spray. Its effects in cannabis are inferred from other crops, so careful experimentation is needed. ### 4. Glutamine Glutamine is a conditionally essential amino acid central to nitrogen metabolism: - **Growth Promotion**: Glutamine (10–1000 mg/L) significantly enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and hibiscus, likely benefiting cannabis vegetative growth. In cannabis, it may support lush foliage and robust rooting in hydroponics.[](https://www.scielo.br/j/oh/a/GZxMgqXb9wm7bsCTv4WwPdc/?lang=en)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html)[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html?lang=en) - **Nitrogen Assimilation**: As a primary nitrogen acceptor, glutamine improves nitrogen use efficiency via the glutamine synthetase/glutamate synthase cycle, enhancing nutrient uptake in cannabis.[](https://academic.oup.com/jxb/article/58/9/2339/544408)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Stress Response**: Glutamine supports proline synthesis, boosting stress tolerance. It also contributes to chlorophyll and protein synthesis, improving photosynthesis and yield potential in cannabis.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) - **Secondary Metabolism**: Glutamine is a precursor to glutamate, which influences GABA, arginine, and proline synthesis, potentially affecting cannabinoid or terpene production indirectly.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2019.01743/full) **Application Notes**: Foliar sprays (250–1000 mg/L) or root application twice weekly are effective. Glutamine at 250 mg/L combined with other amino acids (e.g., glycine) shows synergistic growth effects.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) ### Synergistic Effects on Cannabis - **Growth and Yield**: The combination of EAAs, glutamine, and proline may enhance vegetative growth and biomass. Glutamine and EAAs drive protein synthesis and nitrogen uptake, while proline supports tissue integrity under stress. TMG likely amplifies these effects by improving cellular hydration and methylation, potentially increasing leaf area and bud quality.[](https://www.mdpi.com/2311-7524/8/12/1110)[](https://www.nature.com/articles/s41598-022-21273-6) - **Stress Resilience**: Proline and TMG act as osmoprotectants, while EAAs and glutamine bolster antioxidant systems, collectively improving cannabis resilience to drought, salinity, or temperature extremes common in cultivation.[](https://www.mdpi.com/2311-7524/9/7/807) - **Secondary Metabolites**: Glutamine and TMG may enhance cannabinoid and terpene production by supporting metabolic pathways (e.g., methylation, nitrogen metabolism). EAAs like methionine could influence sulfur-based terpenes. However, direct evidence for cannabinoid enhancement is sparse.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Nutrient Efficiency**: Glutamine and EAAs improve nitrogen and micronutrient uptake, while TMG may enhance nutrient transport, optimizing cannabis growth in nutrient-limited systems like hydroponics.[](https://mycsainc.com/newsletter/en/2023/03/03/amino-acids-and-their-function-in-plants/) ### Potential Risks and Considerations - **Inhibition at High Doses**: High concentrations of proline or certain EAAs (e.g., lysine, valine) can inhibit growth if used as sole nitrogen sources. Balanced mixtures mitigate this, as glutamine can antagonize inhibitory effects of proline or other amino acids.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Nitrogen Overload**: Excessive EAAs or glutamine may lead to nitrogen toxicity, causing leaf burn or stunted growth in cannabis. Monitor plant response and maintain balanced nutrient solutions.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **TMG Metabolism**: TMG may increase trimethylamine production in the soil microbiome, potentially affecting cannabis root health if not managed. Healthy soil microflora can mitigate this.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) - **Application Timing**: Foliar sprays (150–500 mg/L for EAAs, glutamine, proline; 0.5–2 g/L for TMG) during vegetative growth or early flowering maximize benefits. Avoid overuse during late flowering to prevent nutrient imbalances affecting bud quality. ### Cannabis-Specific Insights - **Hydroponics and Soil**: In hydroponic systems, EAAs and glutamine ensure efficient nitrogen delivery, while proline and TMG support stress resistance in fluctuating pH or nutrient conditions. In soil, maintaining microflora is crucial, as it naturally supplies amino acids; supplementation should be moderate.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/)[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **Cultivar Variability**: Cannabis cultivars differ in nutrient demands and stress responses. Sativa-dominant strains may benefit more from proline and TMG for stress tolerance, while indica strains may respond better to EAAs and glutamine for dense bud growth. Testing is needed. - **Secondary Metabolites**: While amino acids influence secondary metabolism, direct effects on THC or CBD levels are unconfirmed. Glutamine and TMG may indirectly enhance terpene diversity via improved photosynthesis and methylation.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) ### Practical Recommendations "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨 - **Dosing**: Apply EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) via foliar spray or root drench twice weekly during vegetative growth and early flowering. - **Combination**: Use a balanced mix (e.g., 250 mg/L EAAs + 250 mg/L glutamine + 50 mg/L proline + 500 mg/L TMG) to avoid inhibition. Test on a small batch first.[](https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2023.1281495/full) - **Monitoring**: Check for signs of nitrogen toxicity (dark green leaves, clawing) or stress relief (improved vigor, greener leaves). Adjust doses based on plant response. "🚨🚨🚨🚨"🚨🚨🚨🚨"🚨🚨🚨🚨. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to enhance uptake.[](https://www.degruyterbrill.com/document/doi/10.1515/opag-2019-0016/html) - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient lockout. In soil, ensure healthy microbial activity to complement amino acid supplementation.[](https://cannabisindustryinstitute.com/news/amino-acids-what-they-mean-to-your-cannabis-grow/) ### Evidence Gaps - **Specific Combination**: No studies directly address EAAs, proline, TMG, and glutamine together in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid Impact**: Limited data on how these compounds affect THC/CBD levels. More research is needed on secondary metabolite pathways.[](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9008891/) - **TMG in Cannabis**: TMG’s effects are extrapolated from other crops. Cannabis-specific trials are needed to confirm benefits. ### Conclusion Applying EAAs, proline, TMG, and glutamine to cannabis plants can enhance growth, stress tolerance, and nutrient uptake, with potential benefits for yield and secondary metabolite production. EAAs and glutamine drive biomass and nitrogen efficiency, proline and TMG bolster stress resilience, and their combination may optimize cannabis health in challenging environments. However, careful dosing and monitoring are crucial to avoid inhibition or toxicity. For best results, test small-scale applications and consult with a horticulturist familiar with cannabis cultivation. If you want more details on application methods, specific cultivars, or further searches for cannabis-related studies, let me know!""🚨🚨🚨🚨 🚨🚨 7/31 125am!!!!! Adding L-glycine to the combination of essential amino acids (EAAs), proline, trimethylglycine (TMG), and glutamine can further influence the effects on cannabis plants, as glycine is a non-essential amino acid with unique roles in plant physiology. Below, I outline the potential effects of L-glycine alone and in combination with the previously mentioned compounds, focusing on their impact on cannabis growth, stress response, and secondary metabolite production. Since specific studies on this exact combination in cannabis are lacking, I’ll integrate findings from plant science and infer synergistic effects based on known mechanisms. I’ll keep it concise and tailored to cannabis cultivation. ### Effects of L-Glycine on Cannabis Plants L-Glycine is a simple amino acid involved in protein synthesis, stress response, and metabolic pathways: - **Growth Promotion**: Glycine (50–500 mg/L) enhances shoot elongation, root growth, and chlorophyll content in plants like lettuce and rice, likely benefiting cannabis vegetative growth. It serves as a nitrogen source and supports protein synthesis, complementing EAAs and glutamine. - **Stress Tolerance**: Glycine acts as a precursor to glutathione, a key antioxidant, reducing oxidative stress from drought, salinity, or high light. In cannabis, it may improve resilience in challenging environments (e.g., outdoor grows or high-intensity indoor setups). - **Secondary Metabolism**: Glycine is a precursor to purines and chlorophyll, potentially enhancing photosynthesis and energy metabolism. It may indirectly influence cannabinoid and terpene production by supporting metabolic pathways, though direct evidence in cannabis is limited. - **Nutrient Uptake**: Glycine chelates micronutrients (e.g., iron, zinc), improving their availability, which is critical for cannabis in hydroponic or nutrient-poor soil systems. **Application Notes**: Foliar sprays or root drenches (100–500 mg/L) are effective, often applied twice weekly during vegetative growth or early flowering. ### Synergistic Effects with EAAs, Proline, TMG, and Glutamine Adding L-glycine to the mix may enhance the effects on cannabis plants in the following ways: 1. **Enhanced Growth and Biomass**: - **Glycine + EAAs/Glutamine**: Glycine complements EAAs and glutamine in protein synthesis and nitrogen metabolism. Studies show glycine (250 mg/L) combined with glutamine enhances shoot and root growth in plants, likely amplifying cannabis vegetative vigor and bud development. - **Synergy**: Glycine’s role in chlorophyll synthesis boosts photosynthesis, supporting the energy demands of EAA-driven protein synthesis and glutamine’s nitrogen assimilation, potentially increasing leaf area and yield. 2. **Improved Stress Resilience**: - **Glycine + Proline/TMG**: Glycine and proline both contribute to glutathione production, enhancing antioxidant defenses. TMG’s osmoprotectant properties further stabilize cells under stress. Together, they may improve cannabis tolerance to drought, heat, or salinity, maintaining growth in adverse conditions. - **Cannabis-Specific**: This combination could be particularly beneficial for outdoor cannabis or indoor setups with inconsistent temperature/humidity, reducing leaf wilting or nutrient stress. 3. **Secondary Metabolite Production**: - **Glycine + Glutamine/TMG**: Glycine and glutamine are precursors to metabolic pathways (e.g., GABA, purines) that may influence cannabinoid and terpene synthesis. TMG’s methyl donation could enhance these pathways by supporting enzyme activity. While direct evidence is sparse, this stack may increase terpene diversity or cannabinoid yield in cannabis buds. - **EAAs Contribution**: Methionine (an EAA) and glycine may support sulfur-containing compounds, potentially enriching terpene profiles. 4. **Nutrient Efficiency**: - **Glycine + EAAs/Glutamine**: Glycine’s chelating properties enhance micronutrient uptake, complementing EAAs and glutamine’s role in nitrogen assimilation. This could optimize nutrient use in hydroponic cannabis systems, reducing nutrient lockout. - **TMG Synergy**: TMG’s role in cellular hydration may improve nutrient transport, amplifying glycine’s effects on micronutrient availability. ### Potential Risks and Considerations - **Inhibition at High Doses**: High glycine doses (1000 mg/L) may inhibit growth by disrupting nitrogen balance, especially if combined with EAAs or glutamine. Studies on Arabidopsis show glycine can be toxic as a sole nitrogen source, so balanced mixtures are crucial. - **Nitrogen Overload**: Adding glycine to EAAs and glutamine increases nitrogen input, risking toxicity (e.g., leaf burn, stunted growth) in cannabis. Monitor for dark green leaves or clawing and adjust doses. - **Competition for Absorption**: Glycine, EAAs, and glutamine may compete for root or foliar uptake, reducing efficacy. Split applications (e.g., glycine in the morning, EAAs/glutamine post-light cycle) can help. - **TMG Interaction**: Glycine and TMG both influence methylation pathways, but excessive methyl donors could disrupt metabolic balance in sensitive plants. Stick to moderate doses (e.g., 100–500 mg/L glycine, 0.5–1 g/L TMG). - **Cultivar Variability**: Cannabis strains (e.g., sativa vs. indica) differ in nutrient and stress responses. Glycine’s benefits may be more pronounced in stress-prone sativas or nutrient-sensitive hybrids. ### Practical Recommendations - **Dosing**: Combine glycine (100–500 mg/L), EAAs (150–300 mg/L), glutamine (250–500 mg/L), proline (50–100 mg/L), and TMG (0.5–1 g/L) in a balanced foliar spray or root drench. Apply twice weekly during vegetative growth and early flowering. - **Application**: Use foliar sprays for rapid uptake during vegetative stages or stress events. Root drenches suit hydroponic systems. Test on a small batch to assess plant response. - **Monitoring**: Watch for nitrogen toxicity (dark, curled leaves) or stress relief (greener, more vigorous plants). Adjust concentrations based on cultivar and growth stage. - **Synergy with Nutrients**: Pair with balanced NPK fertilizers and micronutrients (e.g., iron, calcium) to maximize glycine’s chelating benefits. Ensure adequate magnesium for chlorophyll synthesis. - **Cultivation Context**: In hydroponics, use lower doses to avoid nutrient imbalances. In soil, maintain healthy microbial activity, as microbes naturally produce glycine and other amino acids. ### Evidence Gaps - **Specific Combination**: No studies address glycine combined with EAAs, proline, TMG, and glutamine in cannabis. Effects are inferred from individual amino acid studies and general plant research. - **Cannabinoid/Terpene Impact**: While glycine may support secondary metabolism, direct effects on THC/CBD or terpene levels are unconfirmed. Cannabis-specific trials are needed. - **TMG and Glycine**: Their combined effects on methylation in cannabis are speculative, as most TMG research focuses on other crops. ### Conclusion Adding L-glycine to EAAs, proline, TMG, and glutamine likely enhances cannabis growth, stress tolerance, and nutrient efficiency by supporting protein synthesis, antioxidant production, and micronutrient uptake. The combination may improve vegetative vigor, bud quality, and resilience to environmental stressors, with potential (but unconfirmed) benefits for cannabinoid and terpene production. Careful dosing and monitoring are essential to avoid nitrogen toxicity or absorption competition. Start with low doses, test on a small scale, and consult a cannabis cultivation expert for tailored advice. If you want me to search for more studies, focus on a specific aspect (e.g., glycine’s role in terpene synthesis), or provide application protocols for a particular cannabis strain, let me know!"""

Giorno 1 bene le ragazze si sono riprese bene dal trapianto e stanno sviluppando bene le radici e iniziano a bere più acqua. Al termine di questa settimana verrà pulita bene Giorno 3 LST Giorno 4 leggera defogliazione sotto, annaffiata con 1L di acqua Giorno 7 defogliazione e lollipopping

Hello everyone A fun week again. Plant is showing nice development. I really try to focus on exposing the future budsites. Last year I didn't tuck away the fanleaves enough. This grow I'm tying them all down as much as possible. I find it hard to place them right so every 2 days I do some adjustments. The evolution I have right now is exactly the same as last year so I gotta have faith in the next 2 weeks that she will grow big. Previous grow I had about 60% of the possible canopy. With the right techniques I'm sure I can reach about 80% canopy coverage. I know I'm not the best LST student but I'm trying 😄 Any advice is welcome I'm still giving water with a bit of nutrients but I'm gonna add plain 6,5 PH water from now on. Every second watering during the week plain water. A good rain now would also be very welcome 😄 So far so good Hope everyone is okay See u next week Happy growing 🙏

Flower Week 4 Day 28 to 34 - 4/15 to 4/21 Seeing some purple on the leave and they are really pointing up now... They both look extremely healthy this week. I cant really say enough about the autopot system, the once a week effort is a game changer for me. Feed this week was an auto pot reservoir feed now at 750ppm total before add-ins. I used 750ppm of Bloom concentrate mix (recipe week 5). However, I also added 1ml/gal of CaliMagic (General Hydroponics 1-0-0) and ph balance this week was for 5.8 where Io plan to maintain it until harvest. Next week nutes will decrease in Bloom mix again. The signs of trichomes, pistols, and calyx are all about 3 weeks from harvest now, so feed will be 400ppm before add-ins for with 5.8ph before feeding.