Girls doing well but have run into some calcium deficiency which will hopefully be fixed soon.

D 24 Umtopfen: Topf: 4L airpot, Substrat: Canna terra+ + Kokos fasern (trocken), Wurm Humus: 166,69g+ 50g on top. Bat guano: 15ml. Hornmehl: 15ml. Urgestein mehl: 25ml. Garten kalk: 15ml. Mykorrhiza: 4,20g D24 0,7L Wasser mit Root juice 4ml. D28 Lampe ist auf 40% Leistung total 40W D 28 Die pflanze ist eher Fiming als Topping.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

04.04 F5 04.10 F12

Today harvest the top flower ago. Stopping use nutrition on weekend .

Temperatures this whole week: MAX - 25.8ºC MIN - 18.5º C MAX HUMIDITY - 90% MIN HUMIDITY: 44% Aprox Plants Height Sunday 26: Persian Pie 1 - 23cm Persian Pie 2 - 18cm Persian Pie 3 - 21,5cm Persian Pie 4 - 20,5cm All Gas OG - 14cm Irrigation: 21/3 - 2L (400ml each) of alkaline tap water (6.9 Ph, 127ppm) with 0.1 ml/L of silic boost, 1ml/L of Terra Leaves and 1ml/L of Atazyme. 24/3 - 2L (400ml each) of alkaline tap water (6.72Ph, 117ppm) with 0.1ml/L of silic boost and 0.2ml/L of Rootbastic. (last alkaline irrigation, last dose of rootbastic) -The stains seems to be drops of water from the humidificator, probably they're falling there when i move the deposit to refill it. Next week i should put the light closer, make apical pruning and pull in the tent a scrog web to put a limit to the plants height because they're starting to grow willowy and the tent is only 1.80m tall. I should also make an abundant irrigation to check earth's Ph and PPM again and get the A/C ready to be plugged to the tent because temperatures are rising up exponentially outside and some local growers are starting to have issueswith that. Cheers guys, have a great week and good luck with the outdoor season. Good luck with the harvest to those on the other side of the equator 🙌🙌

Sorry for the late post had a very busy period. The buds are great, good feedback from patients. First time I've been able to taste a fully organic bud and I can say I won't be going back!

This is my second time growing a Girl Scout Cookies autoflower! She turned out amazing! She showed signs of high potency, from early on in flower!

Día 48 . Ya se comienza a llenar la carpa de brotes 🌴, aún falta para pasar a floración 👽. . El led que uso es un TS1000 de Mars-Hydro 🛸🚀 a 17" o 40cm de las plantas. . ▪️Variedad: Big Devil F1 Fast Version ▪️Carpa: 80x80x160 ▪️Temperatura: 28°C ▪️Humedad: 65% ▪️PH agua: 6 ▪️NPK: 8-3-3 : 2ml/litro

Hey everyone! Welcome to our second grow journal! We're growing the same strains as in the first journal, but this time, we plan to only top the plants. They're in 18-liter pots, and you'll be able to follow their progress here as well. These plants are 20 days younger than the ones in the first grow, so it'll be interesting to compare their development. Stay tuned for updates!

have had some temperature problems with the change of the season but i think im dialed in now. this first run really has been trial and error. but so far the buds are swelling nicely. zkittles has more bud sites, and is taller and bushier. blue dream buds are already bigger than zkittles

week 5 when the thrips first showed themselves. I noticed them cuz some silver stains started appearing on some leaves. you can see them better under LED light. So, I sprayed Neem Oil all over the plant. I could notice that if you don't do it well, spraying and rubbing the leaves with a piece of cloth, the oil will also create some stains. 2nd topping. killed some thrips this week, they're cute and didn't seem to harm the plant seriously. Neem oil worked fine to control them. No other pests that I could notice + Defoliation + LST Flowermind (4ml + 0.5g)

A lot of trouble in this round. The problem with the heat contributed to them stretching, and then of course heat stress -.- one of them gave me 20g while the other one 124. I fed them all the same, they may have succumbed to other stress, who knows...

Day 49-56 (June 18th - 25th) (Day 51) Slurricanes and tuna are doing excellent despite their hail damaged leaves. I’m seeing good growth and decent dry downs. One of them looks like it’s been ravaged by pests but I still can’t find any evidence or signs of a pest. I’m suspecting caterpillars so I’m going to grab some BTK killer and also some all purpose insecticide sometime this week if I have time. The clones are all really lagging so they won’t be flipped until July 15th at least. I’m still not seeing normal growth on any of them yet. It’s still quite gnarled and twisted. (Day 52) The slurricane and tuna roots are starting to poke through the bottom of my pots. This is usually from underwatering, a thick soil or a hot root zone. I’m going to give everything except for the autos a good watering with some runoff. I am going to start incorporating 5mm sand into all of my soil mixes to help make the soil more loamy and give it some better drainage. I will also top dress every container and bed plant I have. (Day 53) I was at a local department store today and I was shocked to see pallets of Destiny dark matter soil selling for 60% off! I bought 10 bags for a hundred bucks. At any hydro store that would have costed me 250 at least. I might go grab more because it looks like no one is buying it for 60% off even. (Day 54) I’m going to be harvesting both of my indoor crops today and tomorrow or the next day I am going to be reusing the soil-coco blend for the slurricanes. I’m first going to till it, add a gallon of sea compost, a gallon of Destiny soil and a few cups of 5mm sand per container. I will use the excess for 5 gal pots. I will be amending with: 5 tablespoons of nature’s pride bloom 4 tablespoons of kelp meal 2 tablespoons of Gaia green AP 2 tablespoons of glacial rock dust 2 tablespoons of oyster shell flour 2 tablespoons of alfalfa meal 1 tablespoon of rock dust blend 1/4 teaspoon of langbeinite (Day 56) The autos are really making a comeback now. I was under the impression that I was working against the clock and they would start flowering after 3-5 weeks. I guess if they’re stunted from the cold they will delay flowering. I don’t see any pistils or signs of flowering yet so I don’t think they’ve officially started flowering yet I didn’t get a chance to do my transplant this week or pest control. I will get to it ASAP because my photos are drinking almost a liter a day

Day 79: Second Grow We've decided against using additional nutrients and have completely stopped fertilizing. The light cycle has been reduced to 11 hours, and we're already starting to give them cooler water. Starting Monday, I will be watering with ice water to stress the plants further, aiming for higher yield, more terpenes, and increased frost. The adjustment to the light cycle should also help the plants ripen evenly. The lights are hanging about 20 cm above the buds, which are getting thicker every day, soaking up the light intensely. In about a week, most of the plants should be ready for harvest. We plan to stop watering them for about 100 hours before harvest. During this period, I'll place ice cubes on the soil to shock the roots one last time. 48 hours before harvest, I will turn off the lights, giving the plants two days of darkness. After this period, the plants will be cut and hung to dry.

Another never ending vege week. I took clones and did more trellis work. The PH was up more than usual, they are still alive. I'm glad.

KRITIC AUTO by KANNABIA Week #11 Overall Week #8 Flower This week she just continues to look and smell amazing the colors she has as she's getting close to being done are just 👌 I can't say enough about what KANNABIA has done with the genetics they are working with from autoflowers to photosynthetic genetics they are producing some great stains personally I haven't grown a strain that I haven't enjoyed. Stay Growing!! Kannabia.com. KRITIC AUTO

Day11 Today I did some defoliating. It took me quite some time laying on my stomach😅 I also like a clean growing space as u can see😁