| 📅 Week 9 | Days 57 - 63 | 💡 750w 📏25' | | ☔ 36%-47% | | 🌡️️🌞 74 - 75 | | 🌡️️🌑 73 - 74 | 01/04: I Did a complete reservoir swap on all the plants with an increased nutrient schedule. I should have done this sooner, but I feared the N toxicity. The PH on all the plants has been adjusted to 5.8 as usual. Smells are kicking in really nicely. Great White Mycorrhizae has been added in the reservoirs in place of the Hydroguard. 01/05: Topped off the focus plant only and PHed all the plants to 5.8. The temperatures and humidity has been nearly perfect for my environment. The buds are starting to swell nicely and the trichomes are filling up all over and starting to turn cloudy in some places. 01/06: Reservoirs on all of the plants were topped and and their PH's adjusted to 5.8. No changes have been made for humidity and temperatures. I removed some unhealthy leaves and adjusted plant positions just slightly. Fox tailing has begun to get severe on the focus plant unfortunately with the other two plants acting normal and temperatures around 77 its looking unavoidable and genetic. 01/07: I cleaned out the bottom of tent today from the build up of debris for a clean tent! The reservoirs were topped off and PHed to 6 to combat a dropping PH. If the PH's drop again we will look towards a reservoir swap tomorrow to avoid problems and get a fresh mix. 01/08: Thankfully the PH's have stabilized and aren't dropping drastically so I will not reservoir swap right now and will do it as schedules in a few days. I topped off all of the reservoirs back to normal and PHed them to 5.8. The smell has increased drastically over the week and its amazing right now. 01/09: Applied a small amount of foliar spray to give addition Calmag and keep humidity up today. I adjusted the PHs of all the plants to 5.8 after topping them off with tap water. I also adjusted the SCROG a slight bit and removed some old foliage. The RH levels are still in the acceptable rage for my setup so I will just refill the open containers and not add/remove any of them. 01/10: All of the reservoirs needed topped off and had their PHs adjusted to 5.8. I moved the air ducts around for better airflow. Temperatures and humidity have remained in the good zone for my environment and I look to keep it stable. I moved fans around in the tent to increase canopy airflow. _______________________________________________________________________ This week showed very good bud and trichome development. The addition of Great White Mycorrhizae has immediately increased bud development significantly. The timelapse plant unfortunately has genetic foxtailing on the majority of its buds decreasing overall bud structure vastly. The non-timelapse plants show little to no signs of foxtailing and have nice and chunky bud structures. These plants are maturing EXTREMELY fast over the last week and a half and its looking like harvest time will be much sooner than I anticipated given the lengthy veg time we had. Week 9 overall was pretty rough in terms of growth for our focus plant because the fox-tailing however there is only so much we can do and this week was fantastic for the other two plants so that's always nice! Feel free to leave comments/questions below or give me any feedback to improve my diaries. If you like this Diary follow along and check out my others as well 😎

glad to see her come down and hoping she will dry without mold, not the best looking buds just massive single kolas

Первое, что можно подметить - растение полностью восстановилось. >> Начато полноценное кормление на 2 неделю вегетации! >> Отсчитываем недели…хочется увидеть эту ZZ во всей красе

What is 1st week has gone pretty well after the germination it's got a little bite taken out of it from a tiny bug but other than that it settled in in the soil nicely had its 1st feed with 0.5 mil of expert nutrients grow A 0.5 of grow B 0.5 mil per litre of exponents master root with a ph of 6.5. As I said in the germination update I'm planning to grow this one throughout the whole year until October .

Una cepa muy fácil de cultivar, y muy agradecida de los cuidados y alimentos dados... En sus últimas 3 semanas engordaron muy bien llegando a formar muy lindos apicales centrales.

This is the last week of the Vegetation stage. At the beginning of the week they were trimmed to allow light to penetrate the canopy. Then at the end of the week they were fed a nutrient tea made from Gonzo Farms - GROW. On the last day the lights were set to a 12/12 schedule to induce flowering. Now, I will keep a close eye on them to spot the males. Once flowering begins, the males will grow "bananas". Then, those plants will be removed from the grow. 😜 Stay tuned for the Flowering stage!

Pow Pow Pow what can i say, i mean even the frogs here were hypnotized with this monstrosity size Mama she is all one can ask and so much more, wen harvesting these cutie pie beast Lady i felt the need to break her in small peaces , or smaller that the them baseball bats collas hihihi OMG them colas OMG . She rested for 2 weeks in the rack with temps and RH at 60/60% and she was ready for trimming, trimming her was super easy since her frutes are compact and dense , end up with great trim that i will do extractions asap, have the felling she will give me great returns <3 <3 <3 A true pleasure and delight all the way from seed to harvest , now will came the big and final test and with it the smoke review hehehe, thank you SSSC for the opportunity and for everything and also big shout out to GD without the platform nothing of this wold have been possible <3 <3 <3 l As always thank you all for stopping by, for the love and for it all , this journey of mine wold just not be the same without you guys, the love and support is very much appreciated and i fell honored and blessed with you all in my life <3 <3 <3 #aptus #aptusplanttech #aptusgang #aptusfamily #aptustrueplantscience #inbalancewithnature #trueplantscience #sssc #monstergirl #dogdoctorofficial #growerslove 
 With true love comes happiness <3 <3 <3 Always believe in your self and always do things expecting nothing and with an open heart , be a giver and the universe will give back to you in ways you could not even imagine so <3 <3 <3 Friendly reminder all you see here is pure research and for educational purposes only <3 <3 <3 Growers Love To you All <3 <3 <3 Strain info : About Auto Purple OG Punch cannabis seeds To create Auto Purple OG Punch we crossed our feminized Purple Punch with an autoflower OG Kush. The original Purple Punch is a cross between Larry OG and Grandaddy Purple, illustrious and stable indica dominant THC-rich heavyweights! The total grow cycle from autoflower seed to harvest is a short 9 weeks. You will be rewarded with a large yield of sticky resinous buds, up to 600g/m2 indoors. If you’re looking to grow a high quality indica auto made with cannabis cup winning genetics you will love Auto Purple OG Punch. THC content is always up-to/around 20%. Auto Purple OG Punch effects are intense, euphoric and relaxed. She also contains around 1% CDB which contributes to the very smooth, chilled smoke. Expect a mouthwatering blend of sweet tasting candy, dark fruit and hints of spice. A real connoisseur cocktail with powerful effects! She normally reaches around 1m tall. But in optimized conditions with LED grow lights you can see occasional monster plants up to 2m tall with XXL yields. Auto Purple OG Punch is easy, potent and heavy yielding indoors. She also delivers well outdoors - such as the balcony or garden. Auto Purple OG Punch is a perfect, powerful strain for a quiet relaxed day. The calming indica body effect is very satisfying and de-stressing

Hi all the happy people here in GrowDiaries. This is my second cultivation ever and it will be fun to try a bigger space than my closet grow. First, I'm just going to say I'm done with the construction of my new growroom. I put some pictures on the construction here in week one. The room is 2.14 meters by 1.7 meters and has a ceiling height of 2 meters. It provides a floor area of ​​3.6 square meters. I use a 54 Watt Lightwawe T5 for germination and 2 Pcs 400 Watt HPS lamps. I have a channel fan that replaces the room air about 40 times an hour to get a comfortable theme in the room, the air enters a fresh air intake from the outside. The air is purified through a carbon filter to then leave the room to the rest of the basement. Then I use that heat to heat the rest of the basement. I will use 8 pcs 15 liter Autopots to grow with and a 100 liter water tank that supplies the pots of water and nutrition. I will grow completely organically in soil and will watercure my buds to get the best possible medicine for me. But there are no cultivation rooms to be displayed here, so I continue with what is most important. Today I have put my seeds in my moisture dome and hope the seeds have germinated within a few days. I am very excited to see how the new growroom will work and how this Blue cheese Auto from Royal queen seeds will turn out. Blue Cheese automatic cannabis seeds are a cross of Blueberry Automatic and Cheese Automatic that has been perfected over five years of breeding and careful selection. The result is a feminized, autoflowering strain with superior yield, flavor, and effects to either of its distinguished parent strains. THC: 16% CBD: Low Yield Indoor : 325 - 375 gr/m2 Yield Outdoor: 60 - 110 gr/plant Height Indoor: 40 - 70 cm Height Outdoor: 60 - 110 cm Flowering: 7 - 8 weeks Harvest month: 9-10 weeks after planting Genetic Background: Blueberry x Cheese x Ruderalis Type: Sa 35% In 40% Ru 25% Effect: Cerebral and uplifting Climate: Mild .............................................................................................................................................................................................................................................................................................................................................. Update 2017-08-15. All 4 seeds have germinated and planted in small pots inside the humidity dome. I'm so glad it worked so well and now it's just hoping they'll start growing and become 4 big healthy girls :) ............................................................................................................................................................................................................................................................................................................................................... Update 2017-08-16. I have mixed my own soil today. Its 40% sieved peat harrow H2-H4 0-30 mm, 45% sieved peat harrow H4-H6 0-30 mm. 5% sand and 10% of compost soil. And i use 15% of perilite and mix it all together. ................................................................................................................................................................................................................................................................................................................................................ Update 2017-08.20. Nr1 and 2 grow and thrive. Number 3 just got rid of the seedpod and is folding out the sheets. Number 4 is a bit shy and can only be seen if you look carefully into the soil. ......................................................................................................................................................................................................................................................................................................................................... Update 2017-08-21. Nr 1 and 2 is doing well. Nr3 is trying to catch up and Nr4 is still shy. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-23. Transplanted 2 girls from humidity dome and the small germinating pots to 15 liter autopots. The other 2 girls have to wait a little more before transplant to the autopots. ................................................................................................................................................................................................................................................................................................................................................................................ 2017-08-27. The girls have settled in to there new home of the 15 liters autopot after a little transplant chock. Now the real week 1 starts for me and the girls. -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-28. New pic and movies. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-28. Nr3 is transplanted in 15 liter autopot. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-29. New movie of the girls. Its no water for 3 days now for Nr1 and 2 so the roots develop more and match the plant above the soil. The temp controlled fan is awesome, its easy to set what temp you like to have in the room. Right now its 28 celcius. And humidity is 56%. ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 2017-08-29. Hello to you who read my diary, I just want to say that I am pleased that you have chosen to check in with me and in my diary. I just want to say that I do this for myself and for a steady flow of my medicine. Everything you read and see in my diary is 100% honest and I will never distort or beautify anything here. I document my crops so that I can learn from my mistakes and also to look back at those different crops. I try to update with pictures every day and with text if something special has happened in the garden. This is my strainhunt for the best medicine and the beginning of my journey with cannabis and the cultivation of it. ----------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-30. Cleaned the room this morning, just vacuuming and cleaning with chlorine solution. Im testing the fan to control temp and humidity, it works great. Added some pics with measurements and movie. Everything is looking great right now. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-08-31. New pics and videos. --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-01. New pics and videos. Gave each of the girls with 2 ml Alg-A-Mic, 4ml Bio-Grow, 4ml Bio-Heaven and 10 ml Formulex mixed in 2 liters of water. I hope they like it :) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- 2017-09-02. New pics and the girls liked the water and nutrients yesterday. ------------------------------------------------------------------------------------------------------------- 2017-09-03. New videos of the girls this morning. Nr1 and 3 looks great and nr 2 is looking a little funny, maby a little stressed. Kl. 16.00. Did some defoliation on the girls. Added video. Kl.. 23.00. New videos, defoliation and leaftucking. ----------------------------------------------------------------------------------------------------------------- 2017-09-04. Kl. 11.00. Week 3 starts. Trying to LST the girls and defoliate/leaftuck. New picks. KL 23.00. New video. -------------------------------------------------------------------------------------------------------------- 2017-09-05. KL 08.00. Cleaned the room this morning, just vacuuming and cleaning with chlorine solution. KL 12.00. The girls got 2 liters of water each with nutes in it, added a video. ---------------------------------------------------------------------------------------------------------------- 2017-09-06. KL 08.00. The girls woke up after their beauty sleep, the leaves are always a bit down after 6 h of darkness, but they will stretch now when the HPS lamp starts. Everything looks good and I keep trying to tuck the leaves so they get the maximum amount of light where needed. Added pics and video. The Nr 3 girl is still a little bit behind the other 2 girls and all the 3 girls look like there 3 different pheno of the same plant. KL 23.30. Did some LST. ---------------------------------------------------------------------------------------------------------------------- 2017-09-07. Day 21 from sprouting, Nr3 is 17 days from sprouting. I have never tested PH in my grows, when growing organic in soil iv learned that it adapt PH by it self. But im curious by nature so i had to test. Kl 10.00 Tested PH in the soil, it was 6.9 and tested my tap water and it was 6.8. Added a video of the girls, its 10 H after defoliation, LST and leaftucking. I think its looking good. Kl 22.30. New pics.

se acerca la ultimas semanas, esta semana se dejo de aplicar fertilizante con nitrogeno. se ve una translocacion sana hasta el momento. se espera que en las proximas semanas incremente el nivel de tricomas. Queda poco!!! Pd: pido disculpas a aquellos que siguen de cerca este diario, no pude actualizar a tiempo en las ultimas semanas, espero poder actuaizar de forma oportuna las ultimas semanas.

IVE MADE MORE VIDEOS BUT THEY JUST DON'T SEEM TO UPLOAD AND IT'S FAR TO SLOW TO DO IT FROM HOME 6/27 Made last week a five day week to get back on track. It's still overcast and rainy. It's not raining a lot bit it's consistent. Despite the weather the plants are doing phenomenal. I'll update later. It's 1pm. It's been raining consistently since 11. Just a sprinkle but it's steady. I'm going to begun uploading the weeks weather on my diary. I may start a new diary for the plants I light depped as they are flowering pretty good. Rain stopped and it's just overcast for now. I looked at some videos and did a comparison of videos one week ago and videos today and HOLY SHIT! WHAT A DIFFERENCE. Especially the light depped 10th planet. Well everything but that was the most significant difference. I'm astonished at the health and growth despite the crummy weather. Continued to rain. Just got harder. Plants are taking it but it's flooding underneath the pallets a little but it will be fine. The light dep however has me concerned. The 10th planet is looking spectacular. The bigger purple punch I'd looking good too. The smaller one though looks to have a pollen sack coming off one of the branches. Considering its not on the otherside I assume it's not just a swollen calyx. I don't mind chucking it especially if that means I don't hurt my other girls so I want to make sure. I sent videos to a few other growers and I'll add a question on here. Those three plants have been isolated from the rest for a few days due to rain. I have the suspect isolated alone until I can confirm. It sucks cause the light Depp was going good and the6ve all got little flowers. 6/28 Well that fucking sucks. ALL THREE plants I tried to light depp hermed on me. I could see male flowers. Luckily I had been keeping a really good eye on them and it was preflowers mostly. At least I caught it. One or two stamines on each plant. Would've been really easy to miss. Only one had STARTED to elongate into a stem so I think I caught it early enough. Plus since all this rain they've been kept in a different location then my big girls. Glad I did that now. Boy the roots looked good on those plants. I just grabbed the stalk and lifted and it came right out of the pot. I held it there admiring it for a minute. This sucks. At least the real plants are doing good. As far as I know. No male preflowers that's for sure. I've got some feedback from other growers and the videos are a little blurry but I had found a light leak and I'm certain these plants hermed. I know I could've tried to save them but I didn't want to risk it. I compared what I was seeing with Google photos and other websites. Aside from the larger ball with its stem, there were also several little bumps besides developed calyxes that were weaving into little buds. Trust me that I wouldn't cut down my plants if I wasn't 110% sure. I might've been able to "save them" but to me it's just not worth the risk. 6/29 I was second guessing myself pretty hard last night due to some responses I got on my light dep and messages I got from other growets. Made my anxiety horrible but I looked on several video's I'd taken again and I know what I saw. I felt better after that. This was after I researched and waited THREE days until I saw the ball on the stem and the groupings of small nubs under a fresh yellow flower. These plants were flowering good and it sucks to lose them. One MAY have been ok but one was a runt and had all the characteristics of a true hermaphrodite. They were only in 3's and I couldn't risk my harvest for an experiment. Still sucks. Oh well. Sun is starting to come out. Plants seem to be doing fantastic. I have one spot on a leaf that looks like a pillar munched on a leaf so I'll probably get the bt out soon as I have a dry day that I can apply it. I'll have to check the weather. I need to start a nute regiment but the plants aren't telling me they need anything yet. 6/30 I fucked up dates or dodnt do it yesterday or it didnt save right so I'm leaving this blank today is the 1st. 7/1 I have still only watered s couple times and I haven't had to feed. This week I'm going to start nutes. I had some external ersonal situations that have kept me from my plants. I'm hoping to get back on track. I noticed some pillar damage so I'll need to dig out the BT. This morning I saw this giant ground hog by my cage. Hated too but had to get rid of him. Of course some of the blowback landed on the leaves of one of my plants. I tried to clean it as best I could. Better than that fat bastard eating everything in one night. I broke a branch either falling around it or bulling through when I was pissed or I LST it the wrong way and the wind broke it against the tomato cage. Nice big branch too on top. I tried to fix it with duct tape but we'll see. The plants need me to spend sometime with them. I need to clean them up. Apply bt and give them their first feeding. I'll update as I go. They don't seem nutrient deficient by any means but I don't think it would hurt to start the nutes. 7/2 Bags were lighter today and if it wasn't going to rain tonight and tomorrow I'd he watering. Plants look great so soil isn't depleted yet I guess. They're growing rather rapidly. The branch I broke didnt make it. Had an idea it wouldn't but I had to try. I waited on the BT on account of the rain. I may go back over and change my mind and water with silica or a mild nute solution or maybe apply the BT. Depends what time I get back. I have some work I need to do over there. There's a few that I need to clean up the bottoms on. Pest damage is minor and limited to one or two plants and a leaf or two only. 7/3 More rain. It was supposed to rain this morning too but it didn't. We got .33in yesterday and through last night so I thought that was ok. Looking back on my previous diaries I'm doing things significantly different than before. I had used a lot more nutes earlier on. This morning I mixed two gallons of 2tsp of big bloom and fed it to the 9 plants in smart pots leaving the container plants as they have much more water in them. Looking back at other diaries I previously had, WPM and septoria by this time not to mention a shit ton of other pests I was fighting by this time. Since I poisoned where the cagexwas multiple times and sprayed the cage before it was moved I luckily don't have that problem yet knock on wood. I'm planning to apply BT tonight to deal with the moth larvae if there are any. I'm looking at plants around this area and im seeimg SOME septoria and pm on raspberry bushes and burdock so it is around. I made sure my cage is not by any other vegetation this year and is sitting on asphalt with the bags on raised pallets. Good thing I did or I guarantee they'd be flooded by now. I've been seeing multiple complaints from maine growers online (AND THEY HAVE HEALTHY PLANTS!) saying this is the worst year ever. Maybe they need a dose of fusarium oxysporum to keep them humble. This is maine. If you don't like the weather just wait five minutes. Meanwhile I'll be doing my sun dance hoping for sun. "Hard to grow cannabis with no sunlight" said another grower on my forum.

12-20 Watered both about a gallon. Plain ph. Shoulda maybe added cal/mag . But next time. 3x seemed to burn a little, then some cal/mag issues. 3x is living up to her crosses. She smells like straight gas/fuel. Scc just seems happy. Just took some pictures of that weirdo on her. 😂 Well my first Polyploid, its pretty neat. She is also an oxymoron, she has a sweet stink. 😂 😁😻🤘 12-22 1 gallon Scc only. 5ml cal/mag, 1 ml drops, 1/2 tsp recharge. 12-23 Wanted to double check myself. Ppm 500ish. On 3x. Gave cal/mag 5ml, drops 1ml, and top dressed 1 tbs buildaflower. Might not be enough but we shall see. Happy Holidays 😁🤘 12-24 Watered Scc 3 liters. 1/8th tsp bloom booster, 5ml cal/mag, 1 ml drops. 3x is Hungry AF. May run into some trouble here. 😂 12-26 Scc got ph6.5 bloom booster, cal/mag 5ml and drops 1 ml. 3x is so hungry, I found some tiger bloom I had, idk if its any good. 😂 Gave a bloom booster and some tiger bloom. Was at 700 ppm, then I got scared and diluted it to 550 ppm. Also has some cal/mag and drops. Recharge. Gotta watch her close. 🤞

In week 8 iv come to the sad realization that nearly all of the plants are at different stages of development. Dispite all the seeds being germinated at the same time. So iv had to take them off the wilma systems and am feeding them manually and there all on different diets. Which is an absolute headache especially as there is 16 plants to tend too. I think iv bit off abit more than I can chew for my first grow I maybe should have started with a couple of plants. I think the large wilma setups are designed for clones really so you know they are all going to progress and eat the same amount at once. It's not for autos . There all looking healthy though:)

This week they've stretched a lot , there using a lot more feed now .

Week 3 Great Pistil growth looking forward to day 21 defoliation This one looks really good love the stretch it’s not getting to lanky I’m seeing really fast growth The New. Check out my other Dairies (Gelato Cake& Jungle Verde) Nutrients (floraflex) are in Grams/gal* Light: HLG Follow my IG @therealterpio for more updates & content!

Been a joy too grow this tester , been healthy from start too finnish , green buzz nutrients kept this plant healthy and productive , this plant is slowly begening too ripen , so next week the flush will begin on her , I will hopefully get another 2 weeks out of her yet , so a nice two week flush ,

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Day 21: the next watering will be with the full fert cycle starting week 4. Plants are bouncing back from lst. Day 23: leaf tucking, defoliation and more lst. Day 25: Very hot week, hitting 29°C but the plants are still growing fine