Buenas! Venimos notablemente, tratando de mantener un clima entre 50-60 de H y 24-27 de T. Subimos el Led a 80% y ya le estamos subiendo la dosis de ferti y aditivos. Esta semana ya comenzamos a notar el engorde, y la intensidad de los olores muy marcados. Fenotipo 5: La mas resinosa, internudos mas largos, olor a pomelo y las flores son mas chicas. Puede quedar violeta. Fenotipo 3: cogollo grande y compacto, internodo mas corto, terminando en punta. Olor a tropical, piña 🍍. Fenotipo 4: cogollo muy denso y resinoso, con olor a piña 🍍 tambien pero con un toque banana 🍌. Fenotipo 2: el cogollo super denso y grande, pura resina. Olor no tan fuerte, pero es citrico vomp una naranja 🍊

Week 3 for the strawberry gorilla 🦍 from our favorite autoflower company, of course fastbuds 420! All looks quite good at the moment. This week we gave bloom, root, silica, calmag amino, sugar shot, sticky fingers and bloom booster all from xpert nutrients!

So week 8 flowering, the plant in the back starts to get purple tints (night temperature drops to 12celcius. trichomes on both plants are cloudy (on top) at bottom they have clears left so i go another week and well see what happens. i give only tap water now dont mind the butterfree hes protecting my buds from sneeky neighbours

Hola, buenas a tod@s... Pasamos la séptima semana de esta Red cookie gelato... Buenísima, hermosa planta, punteros y cogollos pretos, tonalidades rosas, purpura, amarillo, luego fuera de la luz es más negra,oscura, es hermosa x donde la veas, mas alla de los signos en sus hojas, esta muy bien y el olor q tiene es espectacular, dulce, afrutado, terrible... Está hermosa... Espero verla ya en la semana final prevía al corte... De momento no hay problema, todo genial .. 👌🏻😎 buen fin de semana para tod@s y buenos humos... 🇦🇷🤝🏻🇪🇦 👍🏻

Growth has been great so far the main crown end up breaking .....see pic nxt week

Hi! I'm actually growing some White Walker Kush from DNA Genetics but I didn't have that option when selecting the strain so I just chose Martian Kush. Sorry about that. It's a seed I found in the weed from my last grow and I wanted to see if I could get sth out of it. Still not sure if it's male or female so wish me luck on this one. Looking good so far :) PS: Right now, I still have the plant in my living room, under a 50W plant bulb with uv, so no strong lights used right now to prevent it from growing too fast. I'll move it into the box and under the 400W as soon as my actual grow is done. Potsize is 65L. I kinda lost track of the weeks since I didn't really wanted to grow this plant so I'll just assume it's around week 9... but I may be so wrong... 👻

Well all these ladies had a major growth spurt. My lights are at the max height now so I hope they are done. Good news though the Gorilla zkittles and Red diesel are showing bud forming, so hopefully I can count on them to be done stretching now. Started them I defoliated pretty good on day 7 I started them on the rhino skin once I got something to keep it turning in my reservoir. I also top dressed 1/2 cup of compost mix and hit them with a light top feed of recharge. Looking forward to seeing these ladies pack on weight in the new few weeks.

I know she yielded less than optimally, but at least she made up for it with a really unique effect. Got like 40 more seeds of this so I'm likely going to do some pheno-hunting come spring with how well she performed. Love the taste and the look of the plant. Really wish I had knowledge of the breeder so I could recommend it. But that's the risk you take with bag seed. Not giving her 10/10 because she stunted for quite a while when I got her started in soil. So she may not respond well with higher stress root-conditions.

Chelsea is doing well. Resin is starting to show on her leaves. Some hairs are showing orange on bud sites. She’s officially taller than my first grow with Durban Poison. I added a bit of Golden Tree this round. Because why not? It’s golden tree!

Prima settimana di fioritura,tutto procede più che bene,,anche questi altri giorni si sta riprendendo ogni giorno di più. ..

cant be happier with the results tbh!!!

Just flip the light schedule to 12/12. Reverse osmosis watering ph 6.2

Just finished week 11, and the time has come! Two of the three ladies were harvested on Monday of this week, the last one is still holding on. Posted some pictures of my homemade odor controlled dry box, the thing works great! Hardly any odors escape when the box is closed up, running a humidifier and drying in the basement has given me a solid 55-65% RH and a comfortable 64-70 degrees fahrenheit for a beautiful slow dry. Once the last one is harvested I will Get her hung up and have updated posts And pictures on harvesting/drying and a final end product. Stay tuned!

Hello to all you growers!!😁 its fist week of blooming my plants.girls strech very nice.the space is full..im soo exited to see buds in the future.watering every second day .the girls are very thirsty🙂

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

08.06.25. Day 39 Moved to bigger tent and 2 100w lights. The lights are weaker but have have more coverage. Plants shot up in last week. Also lots for rain for pass week, so drop in day time temperature, which is good. I will have to do some defoliation later however I don't want to stress plants too much as one was high stressed trained accidentally (branches broke. See last week pics) Thanks for reading. Please leave a like.

What's up Growmies 🤟🏽 Welcome back to another week #11 overall and #3 flowering. She is doing well and continue to stack on buds, I continue to fed her the same schedule and dosage of nutes, it seems to be working well. Everything seems to be going well so far nothing new to report, until next week my friends Happy growing and may the grow goddesses bless you all with a bountiful harvest!

28/04/2021 (Day 43) Se realizo un nuevo cambio de solución, las plantas ya están tirando pelos y los leds están al 80%, se observo un gran crecimiento radicular y las paredes del tallo son increíblemente gruesas y duras. 03/05/2021 (Day 48) Las plantas vienen con un crecimiento optimo pero la flora se está tardando demasiado en hacerse presente, se estima que es debido al estrés que tuvo en semana 3 de vege produjo un retraso de algunos días. Esta semana se incorpora Safe Roots al sistema con la finalidad de que deje la solución lo mas limpia posible. También se realizaron bastantes defoliaciones ya que no existía penetracion de luz teniendo en cuenta el tamaño que están ocupando (85% del espacio de cultivo a 30/35 cm de altura) 05/05/2021 (Day 50) Se realizo un nuevo cambio de solución, esta vez utilizando safe roots para limpiar todo el sistema hidroponico. 600w de Citizen a 30cm en 160x80 y la plantas parecieran que si les doy mas luz van a usarla, es increíble la tolerancia a la alta intensidad de ppfd que tienen, gracias a esto logramos subsanar esa semana de raíces marrones. La temperatura bajo drásticamente y nos dejó el indoor en unos 22 grados, donde todo marcha perfecto.