Day 36 (1/11/21) Nutes: Veg A: 3.83 g/gal Veg B: 2.58 g/gal PPM: 1050 Water temp: 59°F pH: 5.7 Day 37 (1/12/21) Nutes: Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1150 Water temp: 59°F pH: 5.7 Day 38 (1/13/21) Nutes: Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1150 Water temp: 60°F pH: 5.7 Day 39 (1/14/21) Nutes: Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1150 Water temp: 62°F pH: 5.7 Day 40 (1/15/21) Nutes: Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1150 Water temp: 62°F pH: 5.7 Day 41 (1/16/21) Started running a RO:Tap water mix as recommended by Cropsalt FAQ instead of ph adjuster. PPM comes out about 20ppm higher but that’s no problem. Nutes mix: RO:TAP = 15:1 or 120oz:8oz Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1350 Water temp: 62°F pH: 5.8 Day 42 (1/17/21) Starting flower tonight, 12 hours of darkness tonight and for the rest of the grow. Nutes mix: RO:TAP = 15:1 or 120oz:8oz Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1350 Water temp: 60°F pH: 5.8

Clones, week 2. Transplanted into 5 gallon self watering buckets

Nice

Trimmed up the bottoms of the plants after the pics were taken, just forgot to take more. Buds are really starting to pop up everywhere. Currently in NY, and my girlfriend is watching the ladies for a week, so I will try to get her to send me some updated pics. These are from ~5 days ago, and there has been substantial bud bulling since then. They are drinking nutes and water like crazy and get topped off on both every single day lol.

start of fourth week.

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Week 19 and it happens to be a rainy week with on and off cloud coverage which was actually beneficial to my plant but the humidity has been up due to the fact I also use a swamp cooler and if it's gonna be this humid for another couple days then I'm worried about mold developing on the buds so i think I'll break out the AC to get rid of some of that moisture in the air and maybe alternate between the two. The AC hasn't been used once the whole grow cycle so far but living in AZ we get random storms from the monsoon season not to mention tons of dust, high winds, flash flooding which could be used to flush my plant because it needs it now if rain builds up around and gets under the green house, j/k lol. I've been thinking about buying lady bugs to get rid of all the white flies, any experience anybody, would that work? After writing the above, I could hear the wind picking up outside and remembered that a storm was coming so I ran outside to prepare and it punched it's way through Phoenix with extremely high velocity hurricane type wind, gusting up to whatever it takes to bring large trees down or make a wooden shed door fly off with a wall of dust to the sky and then finally finishing with heavy rain. I was outside the entire storm from beginning to end, well at least until the wind died down and it was just light showers by then. Even with having my greenhouse tied down I had to hold onto it for dear life or it would have taken off like in the wizard of OZ. Honest to god I was almost pulled off the ground or several times more onto my greenhouse when the tsunami type wind would hit, pounding me and the greenhouse from behind having to pull down while leaning back with all my weight and strength.The whole time I'm screaming for my brother who was inside to come out and hold the greenhouse so I could tie it down better because it was just coming apart trying trying to fly away or be ripped apart as storm hit us I was holding on for Amy's Green Drop's dear life. It was pretty insane and wish I had video of me doing it but just have a video a friend sent of his backyard getting hit so you can imagine that wind hitting my greenhouse. The after math was bad 😭 the wall of the greenhouse was getting hit so hard by the wind making it press inward towards plant it snapped off in the middle one of the lower branches making the two buds on the end half hanging there from a 90 degree angle from branch so I knew there was no way to support it back in place enough to heal itself so I clipped it off and started drying it. One other next to it got bent up pretty bad so I'm trying to LST it back to place. The storm also killed my swamp cooler somehow, I guess it's because it has a lot of electronic components on top that was soaked from the wind the rain came from the side, I know, you'd think a swamp cooler could get wet. Now I'm stuck using only my A/C and a humidifier to control moisture and temp and I'm worried it's gonna be pretty hard only using AC in such a small greenhouse as it takes time to find the right ratio of intake and outtake air but can only open greenhouse to mix in so much hot dry air, so starting it was getting down into the high 60's, and I have to figure something out because I can't let it get that cold in there for the last few weeks. I'm almost ready to call it quits If I can't find an equilibrium with AC and humidifier, and if there are anymore storms on the way. Next time I'm getting a bigger sturdier greenhouse that goes into the ground and has hard clear walls and roof, other than the recent storm and excessive sunlight finding the right shading it's been very beneficial doing the indoor/outdoor combo grow and would recommend it as you can live in any weathered extreme hot/cold climate and still grow outside,

Ha llegado el momento de cambiar la duración del periodo diurno de nuestro cultivo para inducir la floración. Pasamos a 12h de luz y 12h de oscuridad. Las cuatro luminarias están conectadas al 50% de su potencia máxima. También realizamos una poda baja, para que la planta concentre su energía en la canopia, al mismo tiempo que facilitamos los riegos. Seguimos con la fertilización JUJU Royal by BioBizz, siguiendo su tabla de dosificaciones.

After some research I decided to go for Advanced Nutrients for all amendments. Starting slow and building towards flower, then adding some Bud Candy. These plants really took off lol, shit got out of hand and I let them run a little, I'm definitely a habitual defoliator, I cant stop clipping when I really should just let them be, anyway I moved the tent out of the Closet space as its a friendlier time of year so they don't need the extra protection, kinda why I let them go, Season 1 needed to be smaller in height as they would have suffered outside the tent. Still topped and administered some LST, tried some clips out, but they were far too small and ended up snapping a stem. Would like to give them a go again but will research a bit more next time and not just go for Amazon crap. So far in terms of holding things down I really like the early pegs in the soil approach. I also tried some trellis netting this time, definitely like it and will be more prepared next time. Had to bail for an Easter break so a buddy came through and watered in nuets for me. SKOG was noticeably more Sativa-esque, thinner, stretchier. Runtz with the fatter leaves.

All plants have been chopped down now. Will switch over to harvest on here once these last plants finish drying. Then I can get a full harvest weight of everything. 2 weeks of curing for the first harvest. It is a very fruity smoke. Very sativa high but also a heavy body high. No sleepies or dry mouth. Really enjoying the strain. If I had to guess, I'd say we should be over 2 pounds.

I

Hooked up my carbon filter today. I'm only using it to scrub the air on a timer. Decided to poke a few holes in the ducting to help move scrubbed air around my plant. Added the other fan back in to keep air circulating. Since I don't have fresh air coming in I plan on using a supplement for co2. What are you guys using? It's only a small 2×2×6 tent with one plant and running a 150w HP's light.

Day 30 Feb. 18th- I feel like everything is going great -all plants are now in final 7 gallon containers..have used a lot of soil! -I have been tying the plants down as much as possible -March 2nd will be 42 days and I think that is when I will switch to 12/12 -Day 35 Feb. 24th- I need trellis netting soon I retie branches constantly for inner growth HULKBERRY and GREEN GELATO are growing similar...LSD and ROYAL GORILLA are growing similar! -Very interesting!! * I have color coated the plants like I should have a couple weeks ago -GREEN GELATO has all green pipe cleaners now...HULKBERRY has all yellow pipe cleaners. very long branches and small inner growth LSD is in all purple pipe cleaners. tying it down has done wonders! it has awesome inner growth! Now the ROYAL GORILLA in all orange pipe cleaners! This plant is a pain in the ass lol. The inner growth and spacing is insanely close!!! so many shoots all tightly together. I have tied down anything I can. Unsure how it will work in budding next week

Smell So Good 😊

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Hi Growmies, She did not take well to the ec of 2.0 that I intended to feed her, so I changed the reservoir again midweek and went down to 1.6, after feeding her canna flush with RO water for 24 hours. She seems very forgiving, as she didn’t even get stunted by the lockout. 4 of the 8 shoots went through scrog and were super cropped underneath the screen. The others should catch up any day now and will be trained in a similar fashion. Rootball looks also amazing. I will experiment with continuously feeding her orca every 3 days, as it feels like the mycorrhizal inoculants seem to go down after 3 days and root growth slows down. Let’s see if it’ll work. Here is the code that’ll give you 20% in Zamnesia‘s online store, just input ZAMMIGD2023 at checkout. Thanks for stepping by and until next week!