2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

BEGIN WEEK 8 - Day 50 - 12/11 - Light Water. Day 3 into flowering. Observe red stems. Day 51 - 12/12 - Day 4 into flowering. Red streaks on stem disappearing slowly. Plain Water about a cup. Day 52 - 12/13 - Day 5 into flowering. No Water. Day 53 - 12/14 - Day 6 into flowering. 1.8ml Grow + 1.2 Calmag fed. Red Streaks appear to be stopped growing, looking good progress. Trimmed Fan leaves to expose canopy. Minor adjustment to ties. Day 54 - 12/15 - Day 7 - 1 Week in flowering. Light plain water. About 2 cups. Growth like wild, nice dense green canopy formed. Trimmed lower growth, fan leavs, chopped off weak tops from bottom. Adjusted ties, spaced out the main tops. Day 55 - 12/16 - Day 8 into flowering. Light plain water. Day 56 - 12/17 - Day 9 into flowering. 2ml + 1.2ml Ferts. Plant ajustment and red streaks recovered really good. Overall looking healthy to my rookie vision. END WEEK 8.

Hi all👨‍🌾👋 Welcome to my another week update Hope everyone doing well 🧑‍🌾🤤 Week 3 Feb 16 - Feb 22 It was a very easy week. Both baby girls are growing steady but not in rapid speed yet. 2 waterings of 500 ml each on Feb 17th and Feb 22nd alongside with foliar feedings(100ml water and fish mix) both looking healthy and happy. LST is coming next 😁🧑‍🌾✨🍀 Wishing you all a wonderful week✨🍀 Much appreciate all your likes, follows and comments. 🙏💚❤️💜 Peace and love brothers and sisters 👨‍🌾✌️💚 Links https://2fast4buds.com/seeds/TROPICANA-COOKIES-AUTO https://www.biobizz.com/ https://fishheadfarms.com/

Day 71👋🌱 I started flushing some days befor. I think I’ve got 20 days left but this lady eat a lot of feeding this last weeks so I give some more flushing. Buds looks full of all What they needs also if now they need to get fat and fat. :) let’s see what’s happen. Giving ph controlled water at 6.2/6.5, I check TDS to water owerflowing from pot, bringing it to 50ppm from 400. Now I’ll start checking trichomes every three days, I’d like a 60% white and 40% amber Day 72👋🌱🌱 Hi all growers, how is going? 😊 I'm at my first real flush for this Orange, i gave 10 lt of ph controled water and was checking ppm from water coming out. i gave water unitl it reach 400 ppm, my water is 200 ppm, i hope i'll bring it to 50 ppm for next flush in 3 days. i think bud are going to bloom nice this last days befgore harvest, i can feel it :D Day 74👋🌱🌱🌱🌱 Soil is already quiet dry, smell is increasing rapidly. Today I’ll give another e 1 lt of water and Sunday I’ll do another flush. I think will go up to 90 days. Bud will get fatter and fatter🏆. Maybe I could have fertilise two times more, but anyway I’d not a big problem, I’ve been feeding them a lot since the beginning so a more long flush won’t be a problem at all. Can’t wait to the harvest. Day 77 🌱🌱🌱🌱🌱🌱🌱 Just posting some trichome pictures, waiting to some changement before publishing some new pic of the lady. Keep washing it with ph controleed water that looks always cleaner, i think i'll be cutting the most high bud some days before the rest of the plant. Can't wait anymore Let’se go! ❤️🌱 Any suggestion is really welcome :)😋🌱🌱

🌻 It already starts to smell very well. ✌️🎃Thank you for checking my cultivation.

And here we are at the harvest of our second Jet Fuel, a beauty with a jaunty look, flowers with large calyxes, gassy smell is Jet Fuel by Zamnesia. ** Remember that for all the strains we have at least two plants, one left to grow straight and one worked with the techniques. This is the plant left to grow without the use of techniques to see the other go to the previous week. The trichomes said cut me: it is a sativa predominance of leather we want to preserve the energizing effect. So guys not too much amber on the sativas I recommend otherwise you transform them into indicas. I see enough at least for my tastes and with respect to the strain, always evaluate not only based on your tastes based on the strain. Don't let the amber rule influence you too much we like to talk about harvest window and therefore we have mostly milky / coudy, some transparent and a good amount of amber as well. Technique used // This plant was left to grow free and beautiful with some cleaning at the bottom and some leaves but at the right time. Better with techniques or normal? This time we have two phenotypes similar in appearance and different in terpene profile, the plant left to grow straight seems to be a little better but they are really very similar. Flower quality // From one to 10 stars 9. The plant has resined very well on all sides, it is quite mature, I am satisfied with the color and I harvest it. Buds washed and cleaned as Lukas explains very well on the Zamnesia website, go and read and study the topics in depth, often it is necessary not to act superficially guys! Check the article in your language // https://www.zamnesia.io/it/guida-growing-cannabis/333-lavaggio-delle-ceme Fertilizers // I am very happy with how I managed to unload the plant that at a certain point before going to flush was fed for a few days only with additives and then went to flush and we are very very well discolored, I predict a very tasty flower, grown organic and flushed successfully, the flavor and taste will be epic. All the fertilizers and additives of Plagron in the green / 100% organic version have fully satisfied me. Choose your soil based on your style and calculate the sheet on the website www.plagron.com - Brother if you are stubborn use additives listen every now and then....and then come and tell me. Fertilizers on the other hand are found in convenient all-inclusive packages on the Zamnesia website. Great strain, I found a citrus/sour pheno and a very gassy one ---- https://www.zamnesia.io/en/11258-zamnesia-jet-fuel-semi.html Zamnesia Description // Jet Fuel (also known as G6, Jet Fuel OG and G6 Kush) is a modern hybrid rich in THC and terpenes with a diesel aroma. Originally created in Colorado by crossing Diesel with Aspen OG, this 60% sativa-dominant hybrid is a must-have for any smoker who enjoys an uplifting and energizing high. Now, thanks to the hard work of our breeders, the original Jet Fuel is even easier to grow at home. Order your seeds, plant them in soil and enjoy their rapid growth. After just 9-10 weeks of flowering, you can expect a generous harvest of top-shelf sativa buds! Jet Fuel produces large buds that contain high levels of terpenes and up to 22% THC. True to its name, Jet Fuel has a pungent diesel aroma, with similar flavors. Trained noses and palates will also detect hints of earth, citrus, and pine. Thanks to its potent THC content, Jet Fuel can deliver a very powerful effect after just one hit, making it perfect for social settings, creativity, and daytime use. After an initial burst of energy that increases focus, Jet Fuel's effects tend to settle, leaving users in a dreamy, positive state. Music and Frequencies // For about 4-6 hours a day, my plants listen to 432Hz frequencies that are specifically designed for their well-being, and sometimes a bit of black music to give them that buzz that only music can provide. www.radionula.com All the best that mother nature has to offer is at ---- www.zamnesia.com

Green Cure CBD Auto is making good progress. Her roots are starting to poke out the bottom. She was trained some more today. She got a little hungry and started to get a little pale looking. Fixed that through obvious solution of feeding more. Everything is doing good. Thank you again Zamnesia seeds, and Medic Grow. 🤜🏻🤛🏻🌱🌱🌱 Thank you grow diaries community for the 👇likes👇, follows, comments, and subscriptions on my YouTube channel👇. ❄️🌱🍻 Happy Growing 🌱🌱🌱 https://youtube.com/channel/UCAhN7yRzWLpcaRHhMIQ7X4g

Week 17 Day 113 (04/12/2020)💧: Nice start to the week with a watering. Just as last week, I will be giving them the increased nutrients until the flush. So today I gave them 1.5ml of BioGrow, 6ml of BioBloom, and 6ml of TopMax. Day 114 (05/12/2020): The trichomes were looking beautiful today! (really hard to take close of pictures of them though). You can see in the pictures that some are getting more amber now which is a great sign that the girls are almost ready 🌸 Day 115 (06/12/2020): Took a quick pic of the tent but didn’t have much time to check the girls properly. I’m sure they are great as always 👌 Day 116 (07/12/2020)💧: I watered the girls today with the same nutrients: 1.5ml of BioGrow, 6ml of BioBloom, and 6ml of TopMax. The buds on this girl are looking super dense and even the ones at the bottom are getting bigger! I hope that this 6ml of TopMax will fatten them up even more in the next few weeks before harvest 😄 Day 117 (08/12/2020): Didn’t have time to take pictures today actually Day 118 (09/12/2020): I’ve gotta say that their smell is definitely evolving in a more “mature” direction and they are starting to smell less like citrus and a bit more earthy and spicy. Really incredible! Day 119 (10/12/2020)💧: Watered the girls today with the same nutrients as last time giving them 1.5ml of BioGrow, 6ml of BioBloom, and 6ml of TopMax. They are both doing well but again some of the leaves and stems at the bottom were definitely a bit sad and wilting. But nevertheless, I took some videos and close-up pictures and they look incredible. Their red tint is definitely developing more and more, which is especially noticeable when I take close ups. Also looking from their trichomes, there are some amber ones already so I am thinking to even start the 2 weeks flush from next watering onwards and prepare for harvest! Will assess the trichomes in a few days and decide.

They seemed to drink a lot this week, the environment is stable, and the girls seem to be having a blast. I've checked them throughout and all appeared to be female. Happy growing growmiees

Prima settimana di fioritura in box 60x60 tutto prosegue alla grande dopo essere state travasate in 8 vasi da 6L con terreno miscelato con Lurpe e prima somministrazione di tasty sul substrato innaffiato ogni 3 giorni con soluzione solo Acqua e melassa 300ml a pianta…utilizzo una lampada led fog ho avuto la possibilità di gestire l’altezza gestendo distanza e ppf

Day 43 (1/18/21) Nutes: Veg A: 4.6 g/gal Veg B: 3.1 g/gal PPM: 1350 Water temp: 63°F pH: 5.8 Day 44 (1/19/21) I’m sure my plants have salt buildup, that’s what I’m assuming from leaf symptoms. Starting to flush at lower strength. Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8 Day 45 (1/20/21) Checked ppm of runoff and am very embarrassed it took me so long to diagnose🤦‍♂️ Runoff was 2000ppm Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8 Day 46 (1/21/21) Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8 Day 47 (1/22/21) Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8 Day 48 (1/23/21) Plants seem to be making a comeback Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8 Day 49 (1/24/21) Plants starting to look very good and healthy again 💪 Nutes: Veg A: 3.07 g/gal Veg B: 2.07 g/gal PPM: 775 Water temp: 64°F pH: 5.8

This girl is eager to grow so much so I was able to top her. As she is an autoflower topping is not always the best for the plant, but as I've sniped her nice and early only time will tell. Weather wise it has been a decent week with constant high twenties. Not bad for an English summer. Lol. 😜🤣☔️

Buenas a tod@s... Otra semanita más ya en flora, van creciendo muy bien, uniformes, hermosas y fuertes... Ricos aromas ya están formándose los cogollos de a poquito, van tomando forma de a poco, me encantan, se las ve muy bien y aguantan muy bien la temperatura ya q hay veces q hace bastante calor, se toman otras medidas en ese caso pero de momento van muy bien... A seguir creciendo, me está gustando la evolución de este cultivo, y el siguiente, mejor... 💪🏻⚡💀😎 🇦🇷🤝🏻🇪🇦

У девочки очень плотный и толстый ствол, по этому просела под своим весом см на 5-7, не смотря на то что ещё подсыпал землю постепенно, так же продолжаю каждый 3-4 день проводить дифуиляцию, очень быстро растут новые листья и заполняют всю середину что свет не как не может пробится

the girl is growing very fast :) that's why I decided to remove her wedding leaves, but weak branches, after a week I will let her start blooming on 12/12 :) in some photos you can see such a smile 👨‍🌾, for me it means watering day :D:D.

These are 2 plants, the biggest one in the video is getting flushed today i will harvest her in 5 days and the other plant can stay for 1-2 more weeks

Send help... the Pineapple Express has taken over the tent... she’s massive at 44-45” tall currently... stretch slowed down but she’s past the light at this point and even light angled doesn’t hit the very top of the main colas just yet. (Back left of tent) White widow looking great for the lollipopping method I used on her(front left of the tent) White widow 2 growing well, got a nice dose of ferts this week based on her light coloration(front middle) Berry white growing slowly still, tent is more suited for sativa dominant strains but this one gives the tent a bit more variety. (Middle back of tent) Purple trainwreck 2 growing like a beast this go round, she’s taking up half the tent almost by herself and filling in a 1.5x2 foot area in nicely. (Far right of tent) Purple trainwreck 1 bulking up extremely well, buds are nice and sticky, density is great so far on these buds. Hoping for another 14-20 days. Started her flush after giving her one more dose of nutes last week. Spotted first ambers on the trichomes... she’s only on day 45 of flower but the trichomes are already beginning to turn amber. I still suspect this strain to be semi-auto(has rudelaris genetics somewhere) and additional growing of this plant has simply increased this theory. The plant did have pistils when put into flower(18/6 lighting) but was a clone of a previous plant. She did reveg in 18/6 lighting and was cut from a flowering plant. I believe she had already transitioned into flowering since the plant had started opening pistils up again before being placed in 12/12. I believe she was a week or two into flower at the start of 12/12.

She is a beast

Today is the second day this week with sunshine. The days before were rainy and cold. All plants are doing fine, but I feel, they should be quicker. But then again I am probably spoiled in that regard, by growing indoor. One of the ICE (3) isn't happy somehow and rolling her leaves, but her green and growth matches the others, so I ignore it. The plants received nothing this week, except naturally falling rain water. I have some plant manure cooking, it should be ready in 10-14 days. The garden is full of sweet peas, so i am using those. I also made some compost tea, which I will give the plants tomorrow morning. If there is interest, I will cover my next batch of manure/tea with some pictures. The Passion #1 look like they have bounced back from the topping. In one or two weeks I will top them again.