2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (LIME GREEN CHLOROSIS) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Come walk in the enchanted forest.

Made some defolation, also got rid of some lower buds

Día 75 y último de vida para esta hermosa nena. Me encuentro disfrutando de la manicura y hermosa cosecha que estoy obteniendo con esta planta, mi casa está impregnada por una fragancia dulce con toques tangie. Ahora cortaré por ramas y colgaré en la carpa de cultivo donde se secará por aproximadamente 10 días con humedad y temperatura controlada. Esperaré a curar y probar bien todos esos terps para poder dar un buen informe de cosecha pero hasta ahora todo se ve muy bien! Buenos Humos 👽

It was a very good experience for me because they were most bigger and higher than when you using soil, I didn't expect that)) It was my first time when I used DWC...

right in 2nd week of flower and they have lost their smell? prob because it is so dry and they have started flowering. I gave them bloom amendments last week. lets see if they are in a better place next time i see them. These were clones from a grow buddy but it looks like the pheno was not really deserving of cloning. hope it smokes well.

This week I carry on feed her 5ml bloom 2.5 ml big bud next Week I flush her for 2 week so no much to go Today day 97 and I done last feeding with nutrient now will flush her around 10 days then 2 days darkness then chop her down bud it's not big nut they very heavy and dense hop she give me good yield after long time

Last week think this Sunday coming down

2023-03-15 Please add one more Week in Flowering!- i was in Holidays here This one is kinda perfect strain for me growing vigor and sweet, getting great Strucutr, no Training needed Just love , water and nutes and good Conditions in Light and VPD, made her outstanding I LOOOOVE her Colors in the End, and she smells fbulous, quite Zkittley with alovely Piny undertone, and some Kush Notes. Iam very courious to her taste and effects

Nothing special going on, just keeping humidity and temps low. I’ve started adding Overdrive to the feed. Been checking the trichs a lot. Compared to my other plants, they’re getting really big.

She is growing amazing and very fast. I did some training on the lower branches to get them some light and did some defoliation on unneeded leaves. I’m watering every other day now still only adding nutrients every third watering. I’m ready to see some of these buds start stacking!!!

So fast and so strong, really impressive! Everything is going alright here

Both plants grow with a nice healthy green color and develop well. Barbarian (=AK-47 x Barabara Bud) plant # 2 is a little taller than plant #1 at this time, but both show nice side-branching and overall growth. They love the Advanced Nutrients feeding schedule and are well protected inside the Secret Jardin grow tent.

this grow stretched a t least an extra week my last 2 grows were ripe in 8 weeks .There re no hermi flowers and its going to be at minimum 9 weeks mabe 10 to get ripe .dont know why it stretched into week 6 of flower when its normally 4 weeks stretching the buds are now getting filled in and solid just have to wait i guess

COLOMBIAN JACK by KANNABIA Week #18 Overall Week #7 Flower This week no major issues she's got some nice looking buds on her the aroma coming off this lady is amazing. She's dealt with the elements for the most part!!

Great week

15-05-21 Se cambia la solución y se realiza un lavado de raíces. Para bajar la conductividad de las raíces de 1.43 a 0.2 ms para que la solución nueva sea más efectiva. La solución tiene 2.23 ms y 6 PH. Se añade una pastilla de CO2. 16-05-21 PH 6.2 Conductividad 1.85 ms 17-05-21 PH 6.1 Conductividad 1.84 ms 18-05-21 Se corrige el PH 5.2 a PH 5.9 Conductividad 1.97 ms. Se rellena con agua del grifo ya que el nivel es bajo. Conductividad 0.92 ms 19-05-21 Se corrige el PH 5.6 a PH 6.1 Conductividad 0.97 ms. 20-05-21 Se corrige el PH 5.3 a PH 6.30 Conductividad 1.21 ms. Se rellena con agua del grifo ya que el nivel es bajo. Conductividad 0.74 ms 21-05-21 PH 6.10 Conductividad 0.78 ms

Week 2 of flower and flourishing gave them a full Gaia green feeding after 35 day of veg I'll feed them again 35 days into flower

Prima settimana di fioritura,tutto procede più che bene,,anche questi altri giorni si sta riprendendo ogni giorno di più. ..

Day 54 from seed and the canopy games began, i gave them all some defoliation and instal first net, also started to do some low stress training to guide them out. Day 54 and i had to bring to this side of the tent my little Kushi with is from my other diares but she had no space there, so i moved her next to the competition side 😁😝 I’m also making a water system, i will keep doing the watering manually, but with the help from a “auto/manual” system. Day 54 CS have 2 very distinct phenotipes, a normal one with an amazing black pepper smell with a garlic touch and an old school candy smell mixed within, the other one, with i think is a rare one for the chocolate skunk, is smeling amazingly amazing 😅 she as fruit in it like tangerine and orange, and shes sweet and so but so strong smells, im in love with her, and she stretches more them the other 2 also, she provably will b the keeper from the CS department. Day 54 and I topped CS1 lets see how she reacts 😆🤞💚 Day 54 and TP1 and TP2 are amazing, this girls are so strong and resistant, they are fast growers and they look like winners to me, both of them , caps off to @sweetseeds for such a good job on the genetics department, keep it up guys 🙌🙌🙌🙌🙌🙌🙌 Day 55 and my water system is ready for testing 😅 i know i should test before its all in, but its a non stop live i have so no time , always beliving 😅🤞💚 tomorrow we will know if it works or not 😆😁 Week 8 day 3 and I finally got the water system working and running properly, I’m still plugging in snd out , but this will make my life so much easier, withs results in more free time to dedicate my girls. Tested my run out today and came out with ec over 2.0 meaning next watering mb the 2 nexts ones will be just ph water with regulator and some enzymes. I will keep testing 😆 as for the rest they all look amazing and I’m pretty happy with both genetics , this sad the Tropicanas are way more stable comparing with the chocolates, but still very early in the run 😝😅💚 Little battle between Topicanna Poisen from @sweetseeds vs Chocolate Skunk from @00Seeds (just for fun) TP = Tropicana Poison CS = Chocolate Skunk Light Lumatek Zeus 465W compact pro at 75% All i grow is medecine for myself, nothing to sell, dont even ask !!!! Stay safe and do it with love for the love ❤️ 🙏 💚Growers Love to you all 💚