Big week! With alot to say here!! The first two apace mango that I harvested two weeks ago, finished up drying at 10 days. I trimmed them up today nice and pretty! These were the smallest two plants of this strain in this run, and the yield reflects that. I find that with autoflowers, the bigger the plant, the longer it takes to mature. With the amount of LST I use, my plants tend to take longer than if I were to let them go without lst.. but, its well worth the reward. 1st two harvested yielded 125 grams od high quality, marketable quality flower. After dry period. And 7 grams of high quality, first run, dry sift kief. Next order of business. Harvest 3rd plant on Thursday of this week. (Today is Sunday). I currently have it drying in a tent. (Along with a Northern lights that isnt in a diary). She will dry this entire week, and i may have havest numbera on her when i updated this diary again! Looking 🔥🔥 LAST ORDER OF BUSINESS I hve attached photos of the final 2 space mango from this run. They are by far the largest plants and will be the highest yielding from this run. I expect around 4 ounces from each plant. Give or take. Plants are fading nicely, and i am currently still feeding only water. I have attached photos first of the remaining two space mango plants that are still building. 2nd. I attached photos of the processed flower from the first two harvested on this run.

Even problemen gehad met de ph-waarde, waardoor er waarschijnlijk geen correcte voedingsopname was. Mijn planten werden lichter van kleur en sommige bladeren werden zelfs duidelijk geel tussen de nerven.😕 Ik ga er althans vanuit dat dit het probleem was, aangezien ik eventjes kraantjeswater gaf voor de cal en mag maar dit niet afzuurde. Vandaag testte ik de ph-waarde van het kraantjeswater met mijn nieuwe ph meter en kwam ik uit op 7.2, niet goed voor kokos dus. Ik schakelde meteen weer over naar regenwater, wat met voedingsstoffen in mooi uitkwam op ph 5.8👌 Hopend dat ze zich herstellen, update volgt. De groei gaat gelukkig mooi door!💚 Update 11 mei: 2 planten toonden magnesiumtekort door vorige ph problemen met kapotte meter. Je kan dit nog zien op de foto's tussen de nerven. Zwaar aangetaste blaadjes weggehaald. Intussen kokos verbetert met kieseriet (magnesium) en geen Epsomzout omdat ik dit een lapmiddel vind en liever op lange termijn werk. Na problemen met de ph en substraat gespoeld te hebben, nu ook weer voeding aan het opbouwen👊 Planten voor buiten in een kas, maar hier kreeg ik de luchtvochtigheid niet onder controle (bleef boven de 80 zitten). Ze zijn nu verhuisd naar hun nieuwe verblijf, mijn schuur waar ik altijd kweekte. Buitenkas was een test, maar is dus geen goede optie👎 Op de foto's en video kan je zien dat ze nu veel beter wonen🌱 Update 14/05 : Eerst laten herstellen van magnesiumtekort. Fimmen + LST gestart. Ik leg mijn planten graag plat💚

This is week 10 for our AK-47 Auto Goxuak by Genehtik. She is finishing out great! For the most part she yellowed out but some sugar leaves and caylxs have purple in them. Her buds just keep stacking! Ashoka is already way past her estimated period and still showing white pistils(stoma?) And clear Trichomes.i tried to get some shots.failed mainly lol. When that changes we will chop her. Taking advantage of the longer flower I decided to pull a few of her branches back down to expose the buds to more light. Thicken them up I hope! I flushed her again. This time just in the sink with running tap water(our water is horrible too!) I didn't pH the water or add any Kleanse this week. I also stopped giving her Real Growers recharge. She smells great! Like Pepper and a little skunk.

Week 9, 3 gal transfer into 7 gal....Healthy no pesticides problems or Nutrient deficiencies at all before the flip to

Planta Reagiu super bem ao transplante e voltou a crescer rapidamente. Isso fez com que eu decidisse fazer conforme programado e no último dia da 13 semana (29/01/2020) a 3 lampada (LED 50W 3000K) foi anicionada e o ciclo alterado para 12/12h. Como a terra de transplante já era fertilizada, apenas após 1 mês de flor será alimentada.

Day 43: Watered at 6.5 today. Girls looking pretty good other than a small amount of clawing on some leaves. Thinking I have the fan blasted on them too much. Backed it off a touch and we shall see. Also, my rust spots are starting to come back barely and I think I may up the nutes next go as they are hungry. Stacking up nicely though. Day 44: Busy, no update. Day 45: Girls looking frosty! Think I may have screwed the pooch on one of the girls. Got a little rough picking up the pots and may have snapped a main cola. In the uploaded video notice where its zoomed. Praying! 🙏 Day 46: Letting the girls dry out today. Will give em a thorough watering tomorrow. Not able to upload pics/vids currently. Day 47: No new updates. Day 48: The girls look great! 💪 Gave them a cleanse and just fed with CalMag and Sensizym. Looks like they are finished with the stretch and starting to build mass. Hoping for at least a couple huge colas! Day 49: Looking good. No new updates. I have been keeping a very close eye on the runoff PPM's as I am starting to have some rusty splotches on the leaves again. I have upped the CalMag to see if this helps as that is what it is looking like.

💜 💚 😍🥤💜💜 💚 😍🥤💜💜 💚 😍🥤💜💜 💚 😍🥤💜💜 💚 😍🥤💜 Dear growmies, welcome to week 2!! ❤️ Spraying the babies with Fast Plants Spray every 3 days in the morning, they love it! 💦 Watered once on day 9, added some wee nematodes on day 11 🐛. 💧 Thanks @Fast_Buds for your awesome genetics, growing Fastbuds is always a bit like riding a ferris wheel! Woohooo!! 😜 🙏 💚 Thanks to my sponsors @Green_Buzz_Nutrients, their nutes never ever let me down!! Check out my diaries on how well they work! 💚 😘 In case you want to give them a try, you can find a generous discount code in the setup description further down the road ⬇️ You can find more brand hot updates on my IG, where I share real time events from my growroom, don't miss this! 😝 _______________________________________________________________________ Purple Lemonade FF As refreshing as cold lemonade on a hot Summer day. 🌞 • Quench your thirst. Just as good as lemonade on a hot Summer day. • The gentle giant. Up to 550 g/m2 of gorgeous pinkish-purple buds. • Refreshing for the mind and body. The ideal all-day strain with a perfectly balanced effect. • Sweet n’ sour terps. Mouth-puckering lemon flavors that’ll hypnotize your taste buds. • Properly purple. The perfect choice for purple hash and extractions. As refreshing as cold lemonade on a hot Summer day. Arising from a cross between purple and citrus Cali genetics, Purple Lemonade FF (Fast Flowering) offers a complex high made up of perfectly balanced cerebral and body effects that are ideal for daytime use. Expect an extremely enjoyable high that boosts your mood while deeply relaxing your whole body and getting rid of muscle pain. This meticulous cross produces up to 550 g/m2 of some of the most beautiful purple buds, with pinkish and reddish hues that will catch everyone’s attention. The beautiful purple buds are ready for harvest in 7-8 weeks and come hand-in-hand with unbelievably sugary citrus terps that are just as refreshing as a cold lemonade on a hot Summer day. It’s the ideal variety for the sweet-tooth stoner seeking strains that deliver both in quality and quantity of resin. Bud Description 🦔 Purple Lemonade FF grows chunky, spade-shaped buds that boast a whole range of pinkish-purple hues with rich, dark orange pistils shooting out of every direction. This variety stands out for the, oftentimes, lilac trichomes that give them a gorgeous pink tint and make for outstanding purple concentrates. The buds give off a delicious tart lemon aroma that reveals a sweeter, more fruity scent as you break the buds open. Definitely a must for flavor chasers and those looking for pungent terpene profiles. Smoke Reports 💨 Purple Lemonade FF offers an effect that’s just as unique as the flavor. Expect a potent uplifting high that gives you that energy boost you need to get things done while 100% happy and stress-free. The effect gradually transforms into a wave that works its way along the limbs and through the muscles, deeply relaxing your body and putting you in the right mindset to go through a busy day with a huge smile on your face. This is an excellent all-day smoke as it not only increases energy but can also help combat chronic pain, migraine and stress. Plant Appearance 💄 This feminized photoperiod variety develops a thick and strong structure, growing a medium-sized main cola with multiple shorter side branches, typical of hybrid strains. Purple Lemonade FF develops fairly short internodal spacing with gorgeous purple buds growing stacked on top of each other, making it the perfect choice for growers of all levels looking to get lots and lots of top-shelf purple weed, as this variety can produce up to 550 g/m2 in a 7-8 week flower cycle. Grow Tips 🤓 This is a super fast feminized photoperiod version that takes approximately 7-8 weeks to flower with a 5-week vegetation cycle,, this means you can have faster harvests by shortening the veg cycle or have a longer veg cycle for bigger yields. Purple Lemonade FF (Fast Flowering) makes for a great candidate for growers of all levels as it’s a vigorous strain that will thrive with basic maintenance both indoors and outdoors. This is a resin powerhouse so make sure to have your trim bin close by as all the resinous sugar leaves will make for the most mouth- watering hash and extracts. Flavor 😋 Expect a long-lasting citrus zest that taste just like it smells. Purple Lemonade FF reeks of a delicious blend of sweet fruits and tart citrus that’ll make your mouth water. On the inhale, expect sour flavors that taste just like lemon candy with hints of orange and a really subtle earthy background. And on the exhale, the citrus flavors open up, giving place to a more sugary berry fruitiness that leaves your whole mouth tasting like an extremely sweet weed-infused lemonade. https://2fast4buds.com/seeds/purple-lemonade-fast-flowering _______________________________________________________________ Setup: 140x70x200 cm Spiderfarmer tent 2x Monkey 16W fans (not oscillating) 1x Secret Jardin 30W 1x box fan 50W oscillating (on lowest level) LED Full Spectrum 320W AGLEX AGL-320W-MA 5,6 kg foldable, dimmable 864 pieces Samsung LH301H 864umol/s 2,7umol/J full spectrum white + RED 660nm + IR 740nm wavelength range 380-780nm covering 3x4ft Green Buzz Nutrients https://greenbuzzliquids.com/ ❗ Use code GD42025 for generous 25% discount (for orders of minimum 75€) Biobizz Lightmix + coco + wormcastings + perlite Carbon Active Granulate Filter 125/360 cm³/h PrimaKlima exhaust EC 125-400/240 cm³/h

Harvest: Everything dried up to 347 grams + 96 grams of super high quality trim. Overall I am not too pleased with the smoke of the product. Although the bud size, bag appeal, smell and taste are pretty much all there, the shitty burn is a deal breaker for me and the majority of this weed is quite frankly useless. Another downside is while smoking I’ve found 4 seeds in an ounce from almost every strain. Light leaks? At least I learned a ton, including how to get super good burn for my next crop. I’m hoping that curing for a month or so will improve the burn but I wouldn’t really count on it. I have seen weed dried for 4 days with no cure and no flush smoke clean and not go out once. I have been told that the key to white ashes/clean burn is proper mineral content in the soil and there is tons of scientific studies on cigar ash going back 100 years. Plants grown in a medium with high levels of decomposing organic matter, phosphorus, magnesium, chlorine and heavy metal ratio tend to produce black/grey ash. Whereas plants grown in a medium with a higher ratio of trace minerals like calcium carbonate and potassium in the form of wood ash, rock dusts, oyster shell flour and lower levels of decomposing organic matter will produce a dense, uniform white ash that rarely goes out. But this is still all theory until I test it to confirm for myself a few times. My last few outdoor harvests did not improve with curing at all so I’m hoping this will be the missing link. Plain peat moss and coco clearly lack essential minerals that help burn. If said theory is correct I should find a synthetic nutrient line with more trace minerals. Makes me wonder if growing if rockwool/basalt rock could improve burn in a hydro setup. I think I did a fairly decent job for my first grow. I went wrong in a million different places but I still stuck it out and got some decent herb. Things went wrong early when I got black spot mold, nitrogen/magnesium deficiencies and powdery mildew. I corrected most of it but things didn’t get really bad until mid veg when I intentionally skipped a few feedings and defoliated quite heavily stunting the plants. I also didn’t even top or start LST until the 6-9th node which was really stupid and hurt my yield. My temperatures were all over the place from the learning curve. I was severely overwatering without realizing it. 100% peat moss is a shitty medium. I also fed way too late in flower (not what caused shitty burn). I also used synthetic nutrients in soil. Zkittlez bonsai Yield: 1.9g Smell: 7/10 - sweet, candy, berry, gassy, piney hints Bag appeal: 7/10 - slightly leafy/stemy Crystal coverage: 7.5/10 - very impressed Ash: 6/10 - flaky, mostly white/grey Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 6/10 - taste is decent, needs curing High: 6/10 - indica dom Comment: looks nice, smells nice, tastes nice, burns like shit. 43.5/70 = 62% 👎🗑️ Black Garlic #1 Yield: 53.6 Smell: 8/10 - gassy, piney, earthy, skunky Bag appeal: 7/10 - slightly leafy/stemy Crystal coverage: 7.5/10 - very impressed Ash: 8/10 - thick, uniform white ash Fire holding: 7.510 - stays lit for 45+ sec Smoke: 7.5/10 - taste is good until the end High: 7/10 - sativa dom Comment: looks nice, smells nice, tastes nice, burns great but quite leafy. 52.5/70 = 75% 👍🔥 Black Garlic #2 Yield: 42.3 Smell: 7/10 - woody, earthy, tea Bag appeal: 6.5/10 - slightly leafy/stemy Crystal coverage: 7/10 - impressed Ash: 6/10 - flaky, mostly white/grey Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 6/10 - taste is decent, needs curing High: 8/10 - sativa dom Comment: looks nice, smells nice, tastes nice, burns like shit. 44.5/70 = 63% 👎🗑️ Zkittlez Yield: 39.4 Smell: 7/10 - sweet, candy, berry, gassy, piney hints Bag appeal: 6.5/10 - slightly leafy/stemy Crystal coverage: 7.5/10 - very impressed Ash: 8/10 - thick, uniform white ashes Fire holding: 8/10 - stays lit for seconds Smoke: 7.5/10 - taste is decent, needs curing High: 7/10 - indica dom Comment: looks nice, smells nice, tastes nice, burns great but smalls didn’t fill out. 51.5/70 = 73% 👍🔥 Blackberry Breath #1 Yield: 57.5 Smell: 9/10 - sweet, grape, berry, gassy, earthy, hashy Bag appeal: 8.5/10 - dense, sparkly buds Crystal coverage: 9/10 - super impressed Ash: 5/10 - flaky, mostly grey/blackish Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 5/10 - tastes like chalky shit High: 6/10 - indica dom Comment: looks amazing, smells amazing, tastes like shit, burns like shit. 46.5/70 = 66% 👎🗑️ Blackberry Breath #2 Yield: 76.1g Smell: 6/10 - musky, tea, berry, herbal Bag appeal: 8/10 - slightly leafy/stemy Crystal coverage: 7.5/10 - very impressed Ash: 5/10 - flaky, mostly grey/black Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 5/10 - taste is shit High: 6/10 - indica dom Comment: looks nice, smells shitty, tastes shitty, burns like shit. 41.5/70 = 59% 👎🗑️ Slurricane #4 Yield: 34.5g Smell: 8.5/10 - gassy, deep piney, fuel, earthy, Bag appeal: 8.510 - slightly leafy/stemy Crystal coverage: 7.510 - impressed Ash: 6/10 - flaky, mostly white/grey Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 6/10 - taste is decent High: 6/10 - indica dom Comment: looks amazing, smells amazing, tastes nice, burns like shit. 46.5/70 = 66% 👎🗑️ Badazz OG Cheese Yield: 43.5g Smell: 6.5/10 - musty, woody, cheesy, piney hints Bag appeal: 6/10 - slightly leafy/stemy Crystal coverage: 6/10 - very impressed Ash: 5/10 - flaky, mostly white/grey Fire holding: 4/10 - stays lit for 10-30 seconds Smoke: 6/10 - taste is decent High: 6/10 - sativa dom Comment: looks like shit, smells decent, tastes decent, burns like shit. 39.5/70 = 56% 👎🗑️

Everything went smoothly. Just have a little bit of water around the root every other day or so. Gradually giving more as it grew and the soil dried out.

Sun is coming out. Cut some leafs off. I removed a few, day after day. Hope she likes it😄👨🏽‍🌾 Think she is starting to bloom, add her some BioBizz Bloom to the water.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Day 17 17/07/24 Wednesday De-chlorinated tap water pH 6 with calmag 5ml -5L. Very humid week, noticed a green turning colour on top of soils where the humidity has been far to high. I have installed door netting and recently left open all day and night now so they can condition over night in colder temps. All are doing well, one overdose slightly behind and looking ABIT swifted. But she'll come around 💪💚 Day 19 19/07/24 Friday Lite Feed today, 250ml each pot small run off. Seeing excellent start to these babies. Let's get it 👌💚 Day 21 21/07/24 Sunday De-chlorinated tap water pH 6 with calmag 5ml to 5L. Watering in 1L each day from now. Updated video

Any one know why my leafs are facing downwards

LIVING SOIL DEMETEARTH SYSTEM ⚡-15 % + 2 Seeds ⚡ CODE PROMO : VAN-TERPEN420 https://linktr.ee/vanterpen

Heavy defoliation high stress training end of week two flower, start co2 and canna boost along with canna a and b canna cal mag resin green planet terminator and rhizoctonic E.C 1.4 ph 5.7 humidity 41 happy growing

JOANNE'S CBD / ROYAL QUEEN SEEDS WEEK #15 OVERALL WEEK #3 FLOWER This week all 👍 good no issues to report she's looking good nice and green she's doing her thing 👌 buds are starting to form nicely!! Stay Growing!! Thank you for stopping by and taking a look it's much appreciated!! THANK YOU ROYAL QUEEN SEEDS!! BUDTRAINER.COM BUD CLIPS

Day 106. Girls are really fatten up, even bottom airy buds, get some mass into them on Gorillas. Control Garden is like two weeks behind, so i will feed those 3 longer, all other girls in the middle of this week will get theirs last feed. So approx end of week 18 is chop day for RQS run. Very sad situation, trying not to think about pollination . Never had pollinated garden before, so its hard to make any calls ... Time will show. Day 108. Girls avoided pollination with minimal damage .. Found like 20 real seed spots, hope it will be double that max in total. They are fading and maturing, one more big mistake, left them too bushy , they needed lolitoping higher, loads , millions of airy smallest of all smallest buds ;)) One Cookie going crazy purple .. Pure water and looks like all job is done, still checking for hermies every day ... You search for mistakes instead of enjoying , stupid situation really ... Day 109. Three Gorillas are ready, i don't know how, but calaxies went pinky,violet,grey, didn't mature enough, so really airy and leafy buds.... All pistils are brown and curly Trichs are milky with at least 30% amber. I think they just couldn't make it ; Too weak light, Too small pots, Went too gentle with lolitop, mostly airy shitty outdoor type buds, Had pollination, could stop them from maturing and just putting efforts into seeds. Made mistake with watering almost dried them out on week 5 of flower, after that many pistils died and not many new flowers formed. Bad attempt with CheckPoint intention, back to drawing board ... ;)) Night 110. Girls are ok, smell amazingly, fades slowly but steady. One Cookie went absolute purple, really nice colours, but no buds tho .... And don't know how i did my homework, but after checking seedfinder for flowering time info i don't know how i decided to go with this strains ... Gorillas are amazing, just low yielders. Control Garden is flying, some buds are real size grenades, pure joy to watch them grow ... Happy Growing !