the strain is really strong , low temperatures resist , 3 weeks around 12/16º graus and steell growing wel nice new colos between week 4/6 in flowering. really good strain to manipulate! 5 stars * * * * *

Grow looks good. I will compile only full week videos now. Video be provided by end of week. End of week in this diary is Tue - video expected by Wed. Say 19: first stigmas, I think

Wow... She's really entered flowering this week! I've had to bend over her main cola so is could receive light as opposed to growing above it 😂 😕 Also she's been stretching like crazy. I have backbuilt quite a few bud sites. We will see in the coming weeks how effective this has been.

Day 74: The buds are getting really heavy, and many of the stems a struggling to hold the weight. I have done my best to give them some support, and hope for the best. Also some of the fans are beginning to turn a little yellow, I wonder what could cause that....

Lovely 95

Its starting to look more like a forest from each day that goes on. frost also starting to appear already and we are still early in flower ;) d.66 all good in the hood, ill will try to pull those 2 who are very close to the light more back to the wall, so the lower stalks can get light and space d.66 a little bit of bamboo and lst done without problems 🌞

Week5 Vegetative steering Day 29 ————- 4 minute shots(220+-ml). 50-60klux 68 leaf temp Defoliated/Lollipopped Plants seem to be inefficiently spaced. I moved them all closer and they are now positioned better under the lights ———— Day 30 VPDis about a .95 Vegetative steering schedule for 3gal who drink a little over a quart a day 220 P1 440 P1 660 P1 880 P1 1000 P1 1220 P2 1330(1%)P3 I achieve runoff at end of P1 then do a maintenance shot and finish the day off with a watering. P1 waters every 15 min starting 1.5hours after lights on Plants are swelling. But not as well as when i use CO2. I think plants in flower still need co2 so that we can get these lights closer.(as long as the leaf(temp does not go over 80 and vpd stays good) Also, i found a plant to have hermied in week 2 so i removed the pollen sac and rubbed some pollen on 2 plants. Seems like they are just starting to take form on the flowers. **need to check for hermies beginning week 2** Am happy to get seeds from these ladies as this was very isolated in the grow ________________ Day 31 Plants are starting to frost. Group1 has swelled the most Group 2 has average growth and nice frost Group 3 looks like it was over dosed. *Bras&MJ study shows that MJ does mot need to be sprayed with Bras as it will over stress the plants. Brass was completely fine to spray without having adverse reactions. MJ seems to decrease yields but is a worthy tradeoff(will continue to use)-* ————— Day 32 Sprayed 2 plants with MJ(they now have been sprayed both week 4&6) Sprayed 3 with bras(also have been sprayed twice in flower) Finally frosting On the plants

Terrified, paranoid, I'll put you over everything to fill the void And when you’re gone, will I have anything or will I be destroyed? Uh Feeding 💧 Always foilage with T-rex Shield, MegaKelp+Recharge 25/5 Water 8L+Si 2ml+BioGrow 8ml+TrexGrow 8ml+Calmag 4ml+Whiteroot 4ml ppm530 ph6 27/5 Flush ph6.3 ppm27 29/5 Water 3L+Si 1ml+Calmag 1ml+BioGrow 4ml+TrexGrow 4ml+Recharge1tsp ppm600 ph5.5

Blütewoche 2, einfach wachsen lassen und Dünger leicht erhöhen. In Blütewoche 3 ( Tag 21 ) eine letzte entlaubung geplant und kleine Triebe entfernen.

Hello Growers and Tokers! 👋 👩‍🌾 🧑‍🌾.🔥💨 Sorry for the delay on the weekly updates, had some things I had to attend but I'm back and at it! 21-day defoliation has come.. These pictures were taken on day 20 of flower. So these ladies can get quite bushy with those big indica fan leaves. So a much need defoliation was done. I'm loving the CC2 structure but not so much CC1 & CC3, training with those two got out of hand, had to make so weird ass bends! Distribution is all off with CC1 & 3.. I'll se what I can do moving the colas around to even out the light penetration. The split on both look pretty dope tho and CC3 has that little half loop. I'm thinking about trying a canna bonsai next spring with an autoflower jus because of that half loop. 😂 That sweet sweet cream caramel aroma is getting more and more noticeable. 😍 😍 Trichomes are coming in nicely as well. Pretty happy with the results as always with this strain. Height is different on all three so 77cm is an estimate between the three.. I'm sure buds with start filling in this week after the defoliation done. Only going to be watering with bloom nutrients for the next two weeks then I'll start adding an organic PK booster to the mix. Have a great day! One love!

Another week down I think I've already defoliated before about to bit em again this strain can take alot of stress as with most mephistos I've grown.

Esa familia vamos actualizar ya la tercera semana de floración de estas Lava Cake de Zamnesia. Y es que me sorprende están increíbles tienen un color verde alien se ven bien sanas. Temperaturas máximas en 26.5 y mínimas en 24 y una humedad estable en torno al 50/55%. Os comento que tengo un descuento y para que compréis en la web de Zamnesia de un 20%, el código es ZAMMIGD2023 The discount 20% and the code is ZAMMIGD2023 https://www.zamnesia.com/ Agrobeta: https://www.agrobeta.com/agrobetatiendaonline/36-abonos-canamo Mars hydro: Code discount: EL420 https://www.mars-hydro.com/ Espero que lo disfrutéis, buenos humos 💨💨.

Started the first week of flowering 💚🌱

She got put on 12-12 whilst the photo in the tent flowered and she’s started to show signs of flower

Hello Diary, What a trip. I read a lot about the new F1 hybrid strains when they came out and was really interested to see how quickly they would develop. And indeed, in the end I can say that I am very satisfied with the Medusa F1. I planted three seeds but one did not sprout so two remained on the farm. The first Medusa was ready to harvest 68 days after the seed was placed in the ground, the second Medusa stayed a week longer. All the time the vegetation and flowering grew happily and contentedly. I didn't feed them too many additional supplements and they grew as if they were on steroids. After harvesting the first plant, I put it in a cardboard box to dry to protect it from light since the second plant was still on the farm. Drying lasted 14 days, after which I trimmed the flowers and put them in jars. The final result is 116 grams of dried flowers. Medusa F1 #1 - 47 g. Medusa F1 #2 - 69 g. In the end, I can only honestly recommend everyone to try growing this great strain. Thank you all for your support and see you soon with new F1 strains.

Hello everyone and thanks for stopping by. The start of this journal is a little overdue, but real life gets in the way sometimes and I kept having to delay. I initially had planned to run the Samsquanch OG strain from Mephisto and had even started a journal, but I had some issues starting off and the seeds just didnt take well for whatever reason. I tried to germinate my last 4 of those seeds after the originals failed, but only 1 came up. Still cant figure out what went wrong, but instead of waiting and ordering new seeds I decided to pop these freebies that they game me and the girls had absolutely zero issues and are off to a great start! I transplanted into the net pots on 10/20 (Day 4) after some nice long roots came out. I top fed for a few days until my roots were reaching down through the net pots. Everything is running pretty optimal thus far! Light is set about 40inches away 40% power right now. Daytime Temp is 78f and night is around 72-73f. RH is 62-65% EC 0.5

MIMOSA by ROYAL QUEEN SEEDS Week #17 Overall Week #16 Veg This her last week of veg She's showing signs she about to start the flower phase that's always exciting to see that change. Stay Growing!! ROYAL QUEEN SEEDS MIMOSA

My homework. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration.Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems.