14 days in flower exactly and they are really filling in the space now, plenty of heads on each plant and the centre catchin up nicely.

Week 4 Jan - 10 January 6 January - feed day 10/1 - Last feed

Week 21 and 3

Die gute gewöhnt sich so langsam an Mario aber ob sie wirklich Freunde werden wird sich noch zeigen. Bisher wurde von oben gegossen. An Tag 8 gabs erstmals Wasser von unten (Osmosewasser *Leitwert 30myS und pH 6,3) An Tag 12 wurde getoppt.

Man oh man,without noticing I just found a new favorite strain man.Love the strong fruity and floral aroma coming from these flowers I can't wait to grow her Photoperiod version,it's been a pleasure to grow her,unfortunately I lost her four sisters,I would love to get a lot of full jars of this amazing haze 👃🔝🔝💚

Monday It’s beginning of week 4 and they are doing pretty well. The main stems are hard and strong and it’s impossible to bend them at that point. I am practicing a very light hand LST everyday like I am showing in the video. Tuesday Watering for both of them , you can follow the (simple) process in the videos. The plants are doing great except for a little issue on one of them that I will show you tomorrow. Topping or not ? May be beneficial so I’m about to do it. Fiming maybe ? Thursday Topping on video ! Simple method

Week 9 is coming to an end and it's almost time to harvest. At this point the plants are really loud. I dimmed the light and will probably harvest during week 10. At this point I won't water the plants any more.

First trichomes are getting milky. Getting very sticky as well I love the nice and sweet, fruity smell of her. Can’t believe it made it to this point. Even if it’s not much…extremely happy to look towards my first harvest Update: Decided to cut her next week. Going for high thc-level, just testing it...maybe too early:). Sending her to 48 hours of darkness next Monday

Easy grow, except banane, but result are really good, I'm satisfied for my first Scrog, Next time I'll save 4 branches and not 8 like this run, because the down branches give me not good buds, I've put to the trash around 6 branches with very little bud, so I think it's better to got the same with 4 branches topped to got 8 buds. Thanks for follow and like this diaries, see you on my other grow in Progress, Special thanks to @James and #RQS, for trying this strain and fertilizer 😘.

16.07. Blue Sunset Sherbet Day 78# Nothing special happens except that she showed sex like the other plants. I little late,yesterday was the end of the eleventh week. This plant has fallen on me at least 6-7 times, every time there's a storm I find it lying in the grass and mostly before watering when the soil is light, when it's heavy it doesn't happen... this morning I found it when I was watering, and the storms were yesterday all day and this morning, but I found some damage on the leaves and I'm sure it lay in the grass for a whole day, I didn't go around it, but mostly the wind knocked it down so many times, never a single twig it didn't break, very strong plant...I'll have to move it to another place these days, put stakes in the ground and put it in a net for vegetables. Now I water them every other day. That would be it. Stay High and Keep Growing!!!

About day 38 i am just realizing that i am stupid and been feeding the biogrow for cocoa coir 🤦‍♂️ way iver nuting with soil it says never to go over 1ml/L So i will be flushing for at least 1 or two waterings. Looking for the tip burn to stop spreading and the tips to not look like there clawing

Explosive growth again all round,.. all are flowering now and looking healthy, not going to be like last year's 7ft plants but that's on purpose, roll on next week and hope the sun stays out like it has been because they are loving it at the moment 😀 👍👌

Everything on the readings seem well. Plant two seems to be having some kinda nutrition issue. I'm not sure what it is on the first set it leaves. Plant one is seeming to be super strong, healthy, and green. Gonna just keep watching the water levels and ph and keep on trucking.

Thanks for checking out my grow. 😉👍🏼 Feel free to check out my others, and follow along for more to come. 🌿🌺

|»» LOG DISCLAIMER: Not intended as a grow guide. Grow log entries are usually posted 3 weeks later and are for my learning reference. ««| Introduction - Week 22 Summary - Ensign's log, stardate 17584.1, Been an interesting week in the garden.. At the beginning of the week, decided to increase the soil moisture level a bit. This was huge mistake.. Upgraded the reservoir and raised the height by 2ft. Also refreshed the lung room’s carbon filter. Bud growth was swelling up beautifully. But growth started to droop so switched the soil moisture back, but it was too late. The damage was already done. By then end of the week, lost TKO.. Heady OG is still recovering, but I think she’ll be okay. There were even issues in the veg tent and discovered that two watering units were malfunctioning. Thankfully this issue was fixed and by the end of the week all mothers are back to looking healthy and are thriving! Also gave away the two spare Heady OG clones and been tending to rest with basic RO waterings as needed. More so on the positive side, finally have all that I need to begin my journey into tissue culture and plan to begin soon! Also, even though TKO was lost in the flower tent (damnit Ensign), she is still alive and thriving in the veg tent! So yea. A lot happened this week. Both good and really bad.. Despite the issues, my ganja journey has still just begun and will continue to grow until I succeed. Everything is impossible… Until it’s not. Anyways. Onwards to next grow week. [START OF WEEK 22] ========================================================== ---------- [ 4x4 MAIN TENT ] ---------- Heady OG BX1 #1 [Round4 ~Wk15] - WEEK 22 - Full Bloom Wk6 TKO [Round2 ~Wk20] - WEEK 22 - END [Failure] ----------- [ 3x3 VEG TENT ] ------------ TKO - WEEK 05 - Full Veg Gorilla Goat #1 - WEEK 01 - Full Veg Heady OG BX1 - WEEK 05 - Full Veg Garlic Budder OG#1 - WEEK 01 - Full Veg 09/15/25 → 09/21/25 This Week's Estimated Goals ---------------------------------------------------------------------------------------------- DLI: ~35 mol/m²/d PPFD: ~810 μmol/m²/s VPD: ~1.1 kPa This Week's Meter Readings & Averages ---------------------------------------------------------------------------------------------- Light On/Off: 19:00 - 07:00 | Wattage: ~390.1W [LV05] Distance From Canopy: ~2.5”, 6” DLI: ~39.57 mol/m²/d PPFD: ~916 μmol/m²/s VPD: ~0.88 kPa Soil %: [49.7, 38.6, 34.6, 55.1, 43.3, 42.1, 26.9, 43.6] ========================================================== Day 01 - Monday - 09/15/25 - Total Days: 155 ----------------------------------- Daily Averages ~[+01:00] - Temps: 85.9°F [29.9°C] - Humidity: 80.1% - VPD: 0.85 kPa - Soil %: [49, 38, 31, 52, 43, 41, 22, 43] ----------------------------------- - [+00:36]: Night check on the 4x4 tent. --- Ca-Caw!! Looking great in the garden! --- Both Heady OG (BX1 #1) and TKO are looking healthy and growth is green and thriving! --- Bud growth is swelling up and colas are stacking up with more growth! --- Let’s go my girls! - [+00:40]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 50% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 38% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 30% - [CH03 BT » G20 - TKO] ------ 52% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 44% - [CH05 BL » GR7 - TKO] ------ 41% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 21% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 43% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] --- Since growth has been doing so well, decided to increase the soil moisture a bit! --- Let’s go Ensign! - [+00:43]: Within the Ecowitt App for the WittFlow auto-watering system, --- For Water-F30 » Pheno #1 | [Heady OG BX #1], --- Updated the ON triggers from: ------------ When CH1 soil moisture is UNDER 45% | Top ------------ AND When CH2 soil moisture is UNDER 50% | Bottom --------- Adjusted to: ------------ When CH1 soil moisture is UNDER 50% | Top ------------ AND When CH2 soil moisture is UNDER 55% | Bottom --- Updated the OFF triggers from: ------------ When CH1 soil moisture is OVER 50% | Top ------------ OR When CH2 soil moisture is OVER 50% | Bottom --------- Adjusted to: ------------ When CH1 soil moisture is OVER 55% | Top ------------ OR When CH2 soil moisture is OVER 55% | Bottom --- Applied similar triggers for Water-B20 » Pheno #2 | [TKO], --- Updated the ON triggers from: ------------ When CH3 soil moisture is UNDER 45% | Top ------------ AND When CH4 soil moisture is UNDER 50% | Bottom --------- Adjusted to: ------------ When CH3 soil moisture is UNDER 50% | Top ------------ AND When CH4 soil moisture is UNDER 55% | Bottom --- Updated the OFF triggers from: ------------ When CH3 soil moisture is OVER 50% | Top ------------ OR When CH4 soil moisture is OVER 50% | Bottom --------- Adjusted to: ------------ When CH3 soil moisture is OVER 55% | Top ------------ OR When CH4 soil moisture is OVER 55% | Bottom --- Depending on growth, plan to further increase these levels a bit more later. --- Don’t frack this up Ensign! - [+01:09]: Checked the 3x3 veg tent. --- Ooo yea! Veg garden is looking healthy as well! --- All mothers are vegging along beautifully and clones in tray are looking good and don’t need any watering. --- Easy night in the garden! - [+01:13]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 02 - Tuesday - 09/16/25 - Total Days: 156 ----------------------------------- Daily Averages ~[+01:00] - Temps: 86.3°F [30.2°C] - Humidity: 79.0% - VPD: 0.90 kPa - Soil %: [53, 38, 36, 56, 43, 41, 21, 42] ----------------------------------- - [15:47]: Decided to adjust the soil moisture levels a bit more. --- Within the Ecowitt App for the WittFlow auto-watering system, --- For Water-F30 » Pheno #1 | [Heady OG BX #1], --- Updated the OFF triggers from: ------------ When CH1 soil moisture is OVER 55% | Top ------------ OR When CH2 soil moisture is OVER 55% | Bottom --------- Adjusted to: ------------ When CH1 soil moisture is OVER 60% | Top ------------ OR When CH2 soil moisture is OVER 55% | Bottom --- Applied similar triggers for Water-B20 » Pheno #2 | [TKO], --- Updated the OFF triggers from: ------------ When CH3 soil moisture is OVER 55% | Top ------------ OR When CH4 soil moisture is OVER 55% | Bottom --------- Adjusted to: ------------ When CH3 soil moisture is OVER 60% | Top ------------ OR When CH4 soil moisture is OVER 55% | Bottom --- Will continue to check and adjust these levels as needed. - [16:39]: Evening check on the 3x3 veg tent. --- The veg garden is healthy and wow growth is green! --- All mothers are looking great for the most part! --- The Heady OG mother (front left quadrant) is looking sort of droopy, but growth is green and looking okay. --- The TKO mother (back left quadrant) is bushing out and has just exploded with growth! This particular pheno grows like a tree in veg and her main stalk is already the thickest of the lot. Nice! --- Also glad the newer moms are rooting and getting larger with healthy growth! The original Garlic Budder #1 mother (front right quadrant) is a stretcher and is already getting pretty tall! --- The Gorilla Goat #1 mom (back right quadrant) is also getting bigger and growth is starting to bush out. She’s still the smallest, but should hopefully start to ramp up with growth soon. --- Let’s go girls! - [16:40]: Both of the remaining clones in the propagation tray (Gorilla Goat #2 and the original Garlic Budder #2) look healthy and don’t need to be watered. --- Also gave away the two spare Heady OG BX #1 clones. Hope both thrive with health in their new homes. --- Thanks sisters! Planters of the trees! Ca-Caw! - [19:42]: Checked the 4x4 tent. --- Ca-Caw! Ah yea! I got Headies!! --- Both Heady OG and TKO are looking healthy and wow buds are swelling up! --- Bud colas are everywhere! --- Since I forgot to take readings last week, going to take them now. - [20:39]: Took the weekly height and light distance measurements. ------ Heady OG (BX1 #1) was about 51.5" tall. ------ TKO was about 48" tall. ------ Heady OG (BX1 #1) was about 2.5" away from the grow light (LV5 @ ~390.1W). ------ TKO was about 6" away from the grow light. - [20:48]: Took the weekly general PPFD readings. ------ Heady OG (BX1 #1) was around 916 μmol/m²/s which gives a DLI of 39.57 mol/m²/d under a 12/12 light cycle (LV5 @ ~390.1W). ------ TKO was around 792 μmol/m²/s which gives a DLI of 34.21 mol/m²/d under a 12/12 light cycle. - [20:57]: Quickly checked on the auto-watering system. --- System is still charged and no leaks or kinks found in the tubing. --- Plan to upgrade the reservoir by raising it up a bit. This should help to increase the PSI and waterings more consistant. --- Anyways! - [21:05]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 03 - Wednesday - 09/17/25 - Total Days: 157 ----------------------------------- Daily Averages ~[+01:00] - Temps: 86.8°F [30.4°C] - Humidity: 890.0% - VPD: 0.87 kPa - Soil %: [54, 37, 34, 55, 43, 40, 20, 41] ----------------------------------- - [23:57]: Night check on the 4x4 tent. --- Ca-Caw!! Looking good my girls! --- Heady OG and TKO are looking sort of droopy but bud growth is still thickening up. --- Hmm. --- TKO is looking worse now than at the beginning of the week. - [+00:00]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 60% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 37% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 42% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 40% - [CH05 BL » GR7 - TKO] ------ 40% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 20% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 41% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] --- Decided to reset the soil moisture levels back to the original values. - [+00:01]: Within the Ecowitt App for the WittFlow auto-watering system, --- For Water-B20 » Pheno #2 | [TKO], --- Updated the ON triggers from: ------------ When CH3 soil moisture is UNDER 50% | Top ------------ AND When CH4 soil moisture is UNDER 55% | Bottom --------- Adjusted to: ------------ When CH3 soil moisture is UNDER 45% | Top ------------ AND When CH4 soil moisture is UNDER 50% | Bottom --- Updated the OFF triggers from: ------------ When CH3 soil moisture is OVER 60% | Top ------------ OR When CH4 soil moisture is OVER 55% | Bottom --------- Adjusted to: ------------ When CH3 soil moisture is OVER 50% | Top ------------ OR When CH4 soil moisture is OVER 50% | Bottom --- Applied similar settings for Water-F30 » Pheno #1 | [Heady OG BX #1], --- Updated the ON triggers from: ------------ When CH1 soil moisture is UNDER 50% | Top ------------ AND When CH2 soil moisture is UNDER 55% | Bottom --------- Adjusted to: ------------ When CH1 soil moisture is UNDER 45% | Top ------------ AND When CH2 soil moisture is UNDER 50% | Bottom --- Updated the OFF triggers from: ------------ When CH1 soil moisture is OVER 60% | Top ------------ OR When CH2 soil moisture is OVER 55% | Bottom --------- Adjusted to: ------------ When CH1 soil moisture is OVER 50% | Top ------------ OR When CH2 soil moisture is OVER 50% | Bottom --- Guess less is still more.. Since heat has always been the most limiting factor, more water is not always better.. --- Sorry! Please don’t die on me ladies!! ------ [ » SMOKES A FEW BOWLS « ] - [+00:22]: Checked the 3x3 veg tent. --- The veg garden is looking beautiful and growth is looking healthy! --- Compared to the TKO mother (back left quadrant), Heady OG (front left quadrant) is looking droopy, but she’s still green. --- Wow the TKO mom is looking really healthy with huge fan leaves. Beautiful! --- Both the Gorilla Goat (back right quadrant) and Garlic Budder (front right quadrant) mamas are looking healthy and perky! --- The spare clones (Gorilla Goat #2 and Garlic Budder #2) are looking healthy but need watering. let’s go Ensign! - [+00:26]: Here are the starting initial watering ranges: ------ Gorilla Goat #2 » 255.4g → 300.3g ------ Garlic Budder OG#2 » 256.4g → 300.2g --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight of both clones before watering, ------ Gorilla Goat #2 » 231.2g and was watered 4 days ago. ------ Garlic Budder OG#2 » 252.7g and was watered 6 days ago. --- While on a scale, --- Gently misted the straw mulch of both cups with RO water. --- Focused on the straw and sprayed as evenly as possible. --- Misted 10-20 sprays. Waited a moment, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until both cups weighted at least 300g. --- There was no runoff. - [+00:44]: Took the saturated weight of both clones after watering, ------ Gorilla Goat #2 » 325.3g ------ Garlic Budder OG#2 » 315.6g --- Marked the final wet weight on the back of each cup. Also marked the watering date on the front. --- Placed both phenos back into the tent and back onto the propagation tray. - [+02:06]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 04 - Thursday - 09/18/25 - Total Days: 158 ----------------------------------- Daily Averages ~[+01:00] - Temps: 87.0°F [30.6°C] - Humidity: 80.0% - VPD: 0.88 kPa - Soil %: [48, 38, 36, 57, 44, 43, 27, 43] ----------------------------------- - [10:22]: Okay time to upgrade the reservoir! --- Going to raise the tank up a bit. --- Purchased and received a generic 2ft tall wooden stool from Amazon. --- Time to start building! Let’s go! ------ [ » ~30 MINUTES LATER « ] --- Alright! Stool has been built and she’s pretty sturdy! --- Also decided to refresh and change out the lung room’s carbon filter. The filter is long overdue because I think this will be the first time in this room. --- Also swapped out the lung room’s carbon filter and changed the orientation from horizontal to vertical. --- Now the carbon filter is resting on the table with the exhaust fan point straight up. The slight downside is that exhaust vent is S-shaped which will reduced the efficiency of the fan, but that’s okay. --- As for the carbon filter, both units are refillable! Plan to refresh the old filter unit with new carbon before the next filter swap out (have a bag of fresh carbon pellets). --- Anyways! Now the tank is 24in higher than it was. Since the auto-watering system is gravity fed, this should raise the PSI slightly more. --- This also frees up some floor space under the table. --- While rearranging the Ecowitt WittFlow units, I discovered an issue with two of the units. --- Both the front left (Heady OG BX1 #1 mom) and back right (Gorilla Goat #1 mom) for the veg tent were malfunctioning. --- Shaka.. When the walls fell.. --- Will check on the veg garden this evening. - [16:26]: Evening check on the 3x3 veg tent. --- Okay! Well the garden looks the green and healthy! --- But now the droopy growth from the Heady OG mother (front left quadrant) is making sense.. - [16:42]: Checked all Ecowitt WittFlow units again for water flow. --- Yep. Out of all six units, two are faulty.. --- Both units are giving false readings. Both are connected to the Ecowitt WittBoy WiFi hub and the app seems to be working just fine. --- But when the valves are on in the app, there’s still no water flow. --- Swapped the batteries out on both units and checked the intake tubing. Even used the manual override to open valve. --- Still no water.. --- Need to order new units later.. --- Shaka.. --- Well until the new units arrive, will need to hand water both moms. --- Let’s go! - [17:12]: Filled a pump sprayer with 4 cups of RO water. ------ Added 1/64tsp of Quillaja Saponaria Extract [60] --- Gave the sprayer a good shake before spraying the entire solution onto the Heady OG BX1 #1’s straw mulch. - [17:36]: Filled the pump sprayer with 2 cups of RO water. ------ Added a pinch of Quillaja Saponaria Extract [60] --- Gave the sprayer a good shake before spraying the entire solution onto Gorilla Goat #1’s straw mulch. --- Check on both clones in the propagation tray (Gorilla Goat #2 and the original Garlic Budder #2) and growth looks healthy. Also no need to water! - [17:43]: Checked and updated the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 38% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 57% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 47% - [CH05 BL » GR7 - TKO] ------ 61% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 45% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 50% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] --- Shaka.. When the walls fell. --- Well soil moisture levels all look great now! --- But looking back at my logs, Heady OG’s WittFlow unit started failing about 14 days ago. Also think Gorilla Goat’s unit started failing around a week ago. --- Damnit Ensign!! --- Even though these entries are literally typed and logged for daily grow reference, I completely failed to notice the soil levels dropping from around 41% down to 20% over the past two weeks. --- Wow. ------ [ » SEVERAL BOWLS LATER « ] - [20:05]: Checked the 4x4 tent. --- Nice! Well the garden is looking okay for the most part. --- Heady OG is still looking droopy and yet wow buds are swelling up! --- TKO is looking stressed bud buds look okay. --- Both looked way better at the beginning of the week.. - [20:15]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 05 - Friday - 09/19/25 - Total Days: 159 ----------------------------------- Daily Averages ~[+01:00] - Temps: 87.0°F [30.6°C] - Humidity: 79.7% - VPD: 0.90 kPa - Soil %: [48, 38, 35, 57, 43, 45, 27, 43] ----------------------------------- - [10:41]: Oh what! Nice! Received my order from Plants-In-Jars! --- Ordered both the Plants-In-Jars Starter Kit along with the recently released ‘re-animator’ T-shirt! --- The starter kit includes: --------- AGAR [100g] ------ A high quality agar for plant tissue culture applications. --------- BAP [100 mL] ------ A cytokinin that boosts shoot proliferation and cell multiplication. --------- NAA [100 mL] ------ An auxin that promotes root development. --------- IBA [100 mL] ------ An auxin that accelerates root growth, especially in aroids. --------- MS MEDIA [10 L] + VITAMINS ------ A nutrient-rich medium enhanced with vitamins for superior plant growth. --- Aside from the AGAR Powder, have all the products stored in the fridge. --- Over the past two or so months, been slowly purchasing equipment for the journey into tissue culture. --- Now finally have the plant growth regulators (PGRs) along with the agar to make growth media. --- Almost time to start developing a cannabis tissue culture protocol for the garden! --- ‘5 Products, Unlimited Potential.’ --- The ganja journey continues with tissue culture beginning soon! --- Plan to completely fail for the first few batches, but have plenty of veg stock to perpetually keep trying. --- The Plants-In-Jars starter kit also includes access to a protocol library which has instructions for general tropical houseplants. Plan to start and modify from there. Also have reached out to Laur for basic beginner tips and cannabis specific advice for tissue culture. --- Even with all the current grow issues (also had a recent computer failure), getting hyped for tissue culture! - [23:12]: Night check on the 4x4 tent. --- Wow Heady OG is looking better! Sort of. --- TKO is looking stressed. - [23:17]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 48% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 39% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 56% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 42% - [CH05 BL » GR7 - TKO] ------ 44% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 24% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 42% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [23:19]: Checked the 3x3 veg tent. --- The veg garden is looking beautiful and healthier! --- Nice! The Heady OG mother is looking a lot better and top growth is starting to spread out with perkier leaves! --- Still waiting on the new units so plan to hand water again tomorrow. --- Both the Gorilla Goat #1 and the original Garlic Budder #1 clones (in the propagation tray) are looking healthy and perky! --- Wow Gorilla Goat is stretching! She also needs to be watered. - [23:24]: Here is the starting initial watering range: ------ Gorilla Goat #2 » 255.4g → 300.3g and was watered 2 days ago. --------- General Solo Range: [Dry] «| [Initial Cup Weight] + ~55-100g |» [Wet] --- Took the starting weight before watering, ------ Gorilla Goat #2 » 245.4g --- While on a scale, --- Gently misted her straw mulch with RO water. --- Tried to keep the top growth dry. --- Misted 5-10 sprays. Waited a few seconds, then misted again. --- Made sure to reference the watering weights. --- Repeated this process until the cup weighted at least 300g. --- There was no runoff. - [23:56]: Took the saturated weight after watering, ------ Gorilla Goat #2 » 326.3g --- Marked the watering date on the side of the cup. Also marked the saturated weight on the back. --- Also decided to give her a topping. --- Using a clean pair of trimmers, - [23:59]: Cut and removed a good 5-6inches of top growth. --- Only cut the main stalk. --- Mulched the trimmings. --- Placed her back into the tent and back onto the propagation tray. - [+00:01]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 06 - Saturday - 09/20/25 - Total Days: 160 ----------------------------------- Daily Averages ~[+01:00] - Temps: 85.8°F [29.9°C] - Humidity: 79.3% - VPD: 0.88 kPa - Soil %: [48, 40, 35, 55, 44, 43, 26, 44] ----------------------------------- - [20:06]: Night check on the 4x4 tent. --- Garden is looking okay.. --- Heady OG is looking better and she is definitely looking better. --- But what in Sto’Vo’Kor is going on with TKO!! --- She looks completely fried and yellowed out!? --- All this in less than a week? --- Damnit Ensign! --- Well Heady OG is looking good for now. --- Shaka. ------ [ » SMOKES SEVERAL BOWLS « ] - [20:14]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 45% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 41% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 55% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 47% - [CH05 BL » GR7 - TKO] ------ 42% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 22% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 51% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:17]: Checked the 3x3 veg tent. --- Well the veg garden is thriving so life is still great. --- All mothers are looking healthy and vegging along beautifully! --- Both clones in the tray also look good and no need to water. - [20:22]: Okay! Time to swap out the faulty WittFlow units. --- After installing the batteries, tested both units and everything seems to be functioning properly. - [21:35]: Swapped out both units. --- Also connected each of the new Ecowitt WittFlow units to the WittBoy WiFi hub. --- Then configured each unit with the same watering triggers as the previous ones. - [21:40]: Checked the humidifiers. --- Tanks are still full and no leaks found. Day 07 - Sunday - 09/21/25 - Total Days: 161 ----------------------------------- Daily Averages ~[+01:00] - Temps: 85.2°F [29.6°C] - Humidity: 79.6% - VPD: 0.86 kPa - Soil %: [48, 41, 35, 54, 43, 42, 45, 49] ----------------------------------- - [20:42]: Night check on the 4x4 tent. --- Well Heady OG is looking better! I think. She looks green and less droopy. --- TKO is toasted… The entire back of the tent has yellowed out and leaves are crispy. Shaka.. --- I’ve lost a lot of plants, but this was definitely the biggest one. --- I can’t reach her because Heady OG, so going to just leave everything as is until harvest. --- I guess increasing the soil moisture was a really bad idea.. Damnit Ensign.. --- On the positive side, Heady’s bud growth is still continuing to thicken up! --- Also, glad I have the TKO mother in the veg tent and she’s looking healthy in the veg tent. --- Will probably check Heady OG’s trichomes next week. - [20:48]: Checked the Ecowitt soil moisture sensor data: ------------------ [ 4x4 MAIN TENT ] --------------- ------ 50% - [CH01 FT » G30 - Heady OG BX1 #1] ------ 41% - [CH02 FB » G30 - Heady OG BX1 #1] ------ 35% - [CH03 BT » G20 - TKO] ------ 54% - [CH04 BB » G20 - TKO] ------------------ [ 3x3 VEG TENT ] ---------------- ------ 47% - [CH05 BL » GR7 - TKO] ------ 41% - [CH06 BR » GR7 - Gorilla Goat #1] ------ 50% - [CH07 FL » GR7 - Heady OG BX1 #1] ------ 48% - [CH08 FR » GR7 - Garlic Budder OG#1] --------- Veg: 38% - 45% | Flower: 45% - 55% --------- General: 30% [Dry] «| 45 - 60% [Moist] |» 70% [Wet] - [20:55]: Checked the 3x3 veg tent. --- At least the veg garden is looking healthy and all mothers and clones are green! --- The TKO mother is looking really healthy! She is going to need a topping soon. --- The Garlic Budder OG#1 mother is also starting to get a bit tall and may need a topping soon as well. --- Both clones in the propagation tray are also looking good and don’t need watering quite yet. - [21:00]: Checked the humidifiers. --- Tanks are still full and no leaks found. --- Well another grow week done. --- Failure is always an option and wow what a failure.--- Damnit Ensign. Shouldn’t have increased the soil moisture levels and still can’t believe that increasing the levels by 10-15% was enough to cause TKO to go from green and healthy, to burnt and yellow. --- Since I’m only flowering two plants this cycle, by losing TKO, just lost half the garden. --- Wow. A lot can happen in a week. --- Shaka. When the walls fell.. --- Well at least Heady OG made it through the week and am hoping she survives. --- Anyways, onwards to next. ------ [ » SMOKES SEVERAL BOWLS « ] [END OF WEEK 22]

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.