She's doing great! Just gotta keep up with Ipm. I have been going between circadian sunrise and lost coast pest management. Cannot wait to see this girl flower!

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.

Coming soon

Dam just a beast of an ester and she has these dense hard nugs going ... The water consumption has been high because of the heater I was running .. can't wait for the final product to be done another week and I'll start the two week flush .. the tricohmes are just showing some clear , mostly milky and some amber ..

Not too much to report on this week besides beginning LST. I did a few pistils appearing, may be in pre-flower, but not positive as it’s my first auto. Will progress as normal. Video/photos taken 21 days after breaking soil.

March 3rd, 2019. The plants are 28 days old today. Growing well. They are well in early flower now. Began LST and did just a small amount of defoliating. They had Azos mixed with Mykos on day 26, will probably feed tomorrow depending on moisture, if not the next day for sure. They just look great. March 8th, 2019 - 33 days old. Was a big grow week. Both plants doing great. They had a full feeding Tuesday and today they got a good solid flushing. They are starting to smell pretty good. If you look closely at the cola shot, you can just see Trichomes starting to form. Lord knows what they will look like in 4 weeks. About 16 to 17 inches tall, really heavy branches, leaves look great, nice beautiful green.

Lamp Brightness: 100% @ 16" - estimated 914 PAR (top of GMO 2/back left) Waterings only, daily crop steering - reduced water temp + introduced dry down period IPM once-a-week visual inspection - no foliar applications notes: GMO 1 side branches supported w/ more twine + also showing signs of senescence, darkening buds GMO 2 + 3 fading on upper leaves/branches contemplating harvest next week, trichomes check out great continuing to ripen

I’ ve been really really excited in growing this 🍊. I really loved it to do ti in grow diaries. Community helped me and I hope I’ll be doing always better. I think next time I’ll do a foto to play a little bit more with structure of the plant. Buds smells like orange and cotton, chocolate pinus. Can’t wait to try it. I’ll update in some days when I’ll have some nice buds a also le to sm oke🕹️ Thanks to all the people the followed me and that helped me with important suggestions

Another 19cm vertical growth this week and she got a lot bushier. She has started flowering at last. For nutrients I'm using 15g of 20-20-20 powder mixed with 10L of water. Also added 4ml/L of both Cannazym and Terpinator. This brings it to around 1600ppm including ~300ppm for my clean water. Timelapse Sequence: Blue Dream *** Auto Jack *** (too short - out of shot) Pineapple Express CBD Compassion BLue Dream *** Auto Jack *** (too short - out of shot) Pineapple Express CBD Compassion

I came back after vacation, the girl is ready for cutting, the rainy weather has started, so there is a threat of rot, you can say I managed to find just a few small pits in time and removed them and threw them away, the big and stiff flowers look fantastic:) but after a long trip I was tired, so I didn't take quality photos :( no everything else, I am very satisfied with the result I got:) its smell already lets me know that it will be good:) a lot of sugar leaves from which I will make bubble hash:) I will update the weight when it dries.

Day 63 This plant has been trained into a wide, flat shape, with multiple tops spread out evenly across the pot. The structure is low and bushy, clearly influenced by topping or LST, which has opened up the canopy for better light penetration. Leaf color is generally healthy, a strong green with decent vibrancy, though some variation is visible. The lower leaves show stress — one is yellowed and deteriorating, likely from nutrient deficiency (older growth being consumed) or simply natural fading. The canopy looks vigorous and responsive to training, but growth is still somewhat restrained; the plant doesn’t yet have explosive vertical energy. The root zone may still be recovering from earlier stress, but the overall appearance suggests it’s adapting well. Going forward, the key will be to maintain even watering and steady nutrition, supporting strong root activity. Some light defoliation could help airflow under the canopy and direct energy into the developing tops. If conditions stay stable, this plant should be able to bounce back and continue filling the space effectively.

Hey guys :-) The ladies are developing really well 😊. I'll decide spontaneously whether I'll do topping again because I hardly have any space in the flower tent and I'll soon have to set it to 12 / 12 h due to time constraints :-( . But this week they will definitely get the veggie phase. Was poured 2 times with 1 l each (groundwater see description at the bottom) Everything was made clean. Fresh osmosis water is applied and the plants are sprayed and checked. Stay healthy 💚🙏🏻 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 ‘Powered by GreenHouse Feeding’ Copy the link for 10% off all Nutrients 👇🏼 https://shop.greenhousefeeding.com/affiliate/MadeInGermany_PassionGrower 👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼👇🏼 You can buy this strain at : https://www.amsterdamgenetics.com/product/choco-cheesecake/ 👇🏼👇🏼👇🏼 Use the coupon code: madeingermany for 10% on all Amsterdam Genetics seeds Water 💧 💧💧 Osmosis water mixed with Cal/Mag (24 hours stale that the chlorine evaporates) to 290 ppm and Ph with Ph- to 5.8 - 6.4 MadeInGermany

Temps at 78-84 f, humidity inside dome around 68-76 depending on temps. Direct planting into coco jiffy plugs, charged with a light seaweed extract. Took 2 days exactly for seed to pop out of plugs. Another day to remove its shell, will start counting as day 1 from here on out. Check out my other diaries for the other strains. 1 gelat.og auto (seedsman) and 2 extreme impact auto (heavyweight seeds)

Day 8. From this attemt all 3 made it, but two plants look weakish, from other 2 only 1 seed is out. Not a fantastic start ;) maybe they will catch up later ! Installed tent, light dimmed to 30% of 240W, distance approx 40 cm. Happy Growing !!!

ok so everything has gone fantastic , no nutrient burns at all , and have been slowly creeping up the base nutrients , i have stopped the cal mag and now adding house and gardens bud XL at full strength these girls are now in full swing of flower and i bought a new ph stick this week , i have found when PHing the feed bucket that its best too have air stones in the bucket , add all nuts and then ph but do not use the feed that day as if you check the next day the ph is off again so i left it 24 hours after i add more ph down , and it seems too make the measurements alot easier and stable , i have bent down the main stems " without damaging them onto my netting too A keep the canopy all the same high so my lighting is covering all plants at the same distance and also it allowed the side stems too reach up too , the zkittlez did stay alot shorter and compact compared too the rest , but she has had the shame conditions than the rest , so she much just be a shorter pheno type i also was sponsored another new led light by kingbo this week so have added another 600watt led too the grow , so now have two 600's and one 900watt , and temps are still fine sitting at 24c lights on and 19-20 off , so am pleased with that , this lady stayed very short and compact and didn't really stretch much at all and it by far the smallest plant of the bunch ,

Finish cookies Haze.

So far so good,topped at 1 week.Getting busy with lst soon.👍

I change to a tender and light LED Mars 100wts