26.12.24: These are my 2 best plants P1 and P2 Purple Punch, they are looking great! Not sure what happened to P3. They are in early flower/ pre-flower with the pistils clearly seen on P2. I am also hoping to tie some of the branches down to help light penetration on the lower canopies. I have noticed light stress on my plants. I had moved the lights further away and increased the light intensity to 70%. Unfortunately that created some issues. Namely severe palour of the leaves. To try and rectify the situation, I've dimmed the lights to about 30%, staying at the same distance, about 30 inches away. I measured the par levels, after I did this. They should a reading of anywhere between 40 and 150, at the canopy of the plants. They are all different sizes. This seems to have improved the colouring on all plants. After this evenings watering, I will monitor recovery and increase lighting intensity again slowly. I am also using the light cycle of 21/3, so the plants have many hours more light to absorb, than for example 12/12 or 18/6. I am pleased with the progress, considering all of my mistakes! 29.12.24: So I finally gave LST a shot, it's probably a bit too late, however I really want to get the most of the triploid pheno, and I went a little crazy and LST'd all plants except 2, as they're too small. I did that late last night, this afternoon I was amazed to see the plants turn their leaves back to the light source. I'm glad I overcame my fear of trying it. I'm very excited to see them adapt and progress! 😁 there are videos above with before and after of my LST process. Thanks for checking out my diary 🍃 ✌️

Good

Week 6, 2 weeks outside, first week was a blast, fantastic wheater good night temps. Second week was much colder. Daytime temps just tipping 20 degrees celcius in the sun. Nighttime was between 6 and 7 degrees. Pictures are plant 1 in the shed with transparent roof. Not a real serre, its more just a roof and some protection against most of the elements of nature. Video is plant 2, she's hardening because of the harsh nights. She didnt grow as much as plant 1, but looks healthy, goal number 1 at this moment. Better times are coming so all will settle. 🍀🍀🍀

Both plants are doing very well... One of the two plants is a week ahead and the buds are very firm and compact... The smell is sweet and intense. The stem of the plant is quite thin, I hope the buds won't be so heavy...The reason is I only started fertilizing in the flowering phase I will now fertilize my plant for another week before I start rinsing

Monday, March1st All Seedlings show up... thats fine I spray them daily with Fast Plants spray and they get a little Water too March 5th Today all showing properly up..... one is older than the others but yeah next friday week 2

Great harvest, hope u like it!

Hi guys So end of week 7 entering week 8. Blue cheese stinks buds are solid the dark phoenix scrog is frosty asf!!! They still have a way to go yet tho. I reckon by day 70 all will be well and done There under a 600watt hps light now to finish them off as using the led lights in my pot noodle grow off with wifey. I just started a YouTube channel I will be doing daily videos on my current run, like subscribe and comment on there for shout outs. Happy growing guys! 😁

I will be focusing this diary on the smoothie strain but you’ll be seeing some other plants in the tent that are not the same strain. I only have room in this tent so bare with me. There are 2 Smoothie, 1 CNC, and 1 Stardawg (dog). The smoothie are the two bigger ones in the back of the tent. Now, the Smoothie from FastBuds is just killin it right now. Since I popped the beans they have done nothing but show signs of greatness. I don’t think this one is gonna slow down much either. I’m going to push these plants harder than my last harvest. I had a really really amazing harvest last time. I was even able to pull sap out of all 4 plants. 2 Zkittles and 2 LSD-25. This was all done by feeding at the right times and keeping a “moist” soil. Also I want add that I ran pretty much the entire line of Nectar for the Gods at a little less then the recommended ratios. This time I plan on going a tiny bit over the recommended ratios just to see what these plants will do. Trust me, if the plants have a bad response I will go back to the recommended ratios. The reason I want to do this is because I really think these auto strains can handle a lot more than a regular flowering cycle plant would. They can handle more stress, that’s for sure. When do you think I should add a compost tea into my regimen? Soon or wait till the plant is a little larger?

Good weather here. Almost total defoliation and stop watering on Sunday. Cropped on Tuesday night. I'm more than satisfied. A lot of stuff. Perfectly ripen. It's a sin to cut her, she's really a beauty. Top cola apart there are another 12 big bus and 2 dozen of minor fat branches. After 24h of drying and deep wet trim the weight is 1335 grams (2.94 lbs) of top rock solid buds as this plants don't produce popcorns. So it's legit to expect 600 grams of premium weed. See you in 2/3 week for the harvest report, drying will take more time than Royal Gorilla as buds are Biggerrr Smoking preview Folks, I just rolled a couple spliffs of a dried and cured sample bud for 11 days. Two of friends of mine couldn't tell if it was weed or hash, I'm not joking. Strong and fast high.

This thing is still on a freight train it’s about double it’s 2 partners and almost as dense as the blue dream. This week it started its stretch it’s gonna be so beastly

Growing good so far .. she gets sad very easily other than that she’s great the smell I love ❤️ she smells like sweet lemon tea amazing

Bueno por fin la cosecha ha salido bien aun que no ha salido realmente como quería pero a pesar de eso estoy contento con muchas ganas de probarla ya

Week 10 total life Week 5 flowering The stretching stopped and the energy is spent on bud formation. Fertilizer. Plagron Boom 5 ml, power bud 1 ml and green sensation 1 ml Stopped the vita race this week. Happy with the way it's building up it's bud density. leafs and flowers are turning purple and the resin production is great......4 weeks to go...can't wait 😶‍🌫️

Started the final week of nutrients. Then ill be switching to flawless finish and pure water the last week. The blue gelato big cola is finally starting to stack up. It has only one more week of nutrients, so hopefully it stacks quick.. Then a 1week flawless finish and a week of straight 100ppm water.

Dans l'ordre: 1) Afghan Peach x Blue Monkey 2) Gelato Cake 3) Fast Critical Poison 4) Tropical Fuel 5) Hindu Kush 6) (Blueberry x Black Domina) X (Kosher Kush x Mk-Ultra) 7) Blueberry 8) Herz OG 9) (Blueberry x Black Domina) X (Kosher Kush x Mk-Ultra)

Opium

Let’s go day 43!!!! Week went real well , girls started preflower so sometime this week I will be switching up the nutrients for flowering! We are stable and looking super healthy! Can’t wait to see what these ladies do this week! Hope you all enjoy !

Flowering has started.

Both ladie growing very well and are like twin same size leaf development

2nd net is up. Early bud formations are promising. holding up to the extremes pretty well, some leaves taking minor damage, but overall, she is holding up, gave her 1 night at 50F see how she would react, stressful. Not advised as it messes with her metabolism, but I want to see if it triggers any anthocyanin response. Love to see her purp up but no signs yet. Remember, For every molecule of glucose produced during photosynthesis, a plant needs to split six molecules of water. This process provides the hydrogen needed for synthesizing glucose and other organic compounds, while oxygen is released as a byproduct. Homework. If Rubisco activity is impaired and it cannot properly function or regenerate its substrate, the plant's leaves are likely to turn a pale green or lime green, a condition known as chlorosis. Essentially, Rubisco activity is highly regulated and susceptible to various environmental and metabolic factors that can cause it to become inhibited, leading to an apparent failure in RuBP regeneration due to a lack of consumption. Rubisco regeneration is intrinsically linked to nitrogen supply because Rubisco is a major sink for nitrogen in plants, typically accounting for 15% to over 25% of total leaf nitrogen. The regeneration phase itself consumes nitrogen through the synthesis of the Rubisco enzyme and associated proteins (like Rubisco activase), and overall nitrogen status heavily influences the efficiency of RuBP regeneration. RuBisCO is a very large enzyme that constitutes a significant proportion (up to 50%) of leaf soluble protein and requires large investments in nitrogen. Insufficient nitrogen supply limits the plant's ability to produce adequate amounts of RuBisCO, thereby limiting the overall capacity for photosynthesis and carbon fixation. Maintaining the optimal, slightly alkaline pH is crucial for the proper function and regeneration of Rubisco. Deviations in either direction (too high or too low) disrupt the enzyme's structure, activation state, and interaction with its substrates, leading to decreased activity and impaired RuBP regeneration. (Lime/yellowing) Structural Component: Nitrogen is an essential building block for all proteins, and the sheer abundance of the Rubisco protein makes it the single largest storage of nitrogen in the leaf. Synthesis and Activity: Adequate nitrogen supply is crucial for the synthesis and maintenance of sufficient Rubisco enzyme and Rubisco activase (Rca), the regulatory protein responsible for maintaining Rubisco's active state. Nitrogen deficiency leads to a decrease in the content and activity of both Rubisco and Rca, which in turn limits the maximum carboxylation rate, Vmax, and the rate of RuBP regeneration Jmax, thus reducing overall photosynthetic capacity. Nitrogen Storage and Remobilization: Rubisco can act as a temporary nitrogen storage protein, which is degraded to remobilize nitrogen to other growing parts of the plant, especially under conditions of nitrogen deficiency or senescence. Nitrogen Use Efficiency (NUE): The allocation of nitrogen to Rubisco is a key determinant of a plant's photosynthetic nitrogen use efficiency (PNUE). In high-nitrogen conditions, plants may accumulate a surplus of Rubisco, which may not be fully activated, leading to a lower PNUE. Optimizing the amount and activity of Rubisco relative to nitrogen availability is a target for improving crop NUE. Photorespiration and Nitrogen Metabolism: Nitrogen metabolism is also linked to the photorespiration pathway (which competes with carboxylation at the Rubisco active site), particularly in the reassimilation of ammonia released during the process. To increase RuBisCO regeneration, which refers to the process of forming the CO2 acceptor molecule Ribulose-1,5-bisphosphate (RuBP) during photosynthesis, the primary methods involve optimizing the levels and activity of Rubisco activase (Rca) and enhancing the performance of other Calvin-Benson-Bassham (CBB) cycle enzymes. Biochemical and Environmental Approaches: Optimize Rubisco Activase (Rca) activity: Rca is a crucial chaperone protein that removes inhibitory sugar phosphates, such as CA1P (2-carboxy-D-arabinitol 1-phosphate), from the Rubisco active site, thus maintaining its catalytic competence. •Ensure optimal light conditions: Rca is light-activated via the chloroplast's redox status. Adequate light intensity ensures Rca can effectively maintain Rubisco in its active, carbamylated state. •Maintain optimal temperature: Rca is highly temperature-sensitive and can become unstable at moderately high temperatures (e.g., above 35°C/95F° in many C3 plants), which decreases its ability to activate Rubisco. Maintaining temperatures within the optimal range for a specific plant species is important. •Optimize Mg2+ concentration: Mg2+ is a key cofactor for both Rubisco carbamylation and Rca activity. In the light, Mg2+ concentration in the chloroplast stroma increases, promoting activation. •Manage ATP/ADP ratio: Rca activity depends on ATP hydrolysis and is inhibited by ADP. Conditions that maintain a high ATP/ADP ratio in the chloroplast stroma favor Rca activity. Enhance Calvin-Benson-Bassham (CBB) cycle enzyme activity: The overall rate of RuBP regeneration can be limited by other enzymes in the cycle. •Increase SBPase activity: Sedoheptulose-1,7-bisphosphatase (SBPase) is a key regulatory enzyme in the regeneration pathway, and increasing its activity can enhance RuBP regeneration and overall photosynthesis. •Optimize other enzymes: Overexpression of other CBB cycle enzymes such as fructose-1,6-bisphosphate aldolase (FBA) and triose phosphate isomerase (TPI) can also help to balance the metabolic flux and improve RuBP regeneration capacity. Magnesium ions, Mg2+, are specifically required for Rubisco activation because the cation plays a critical structural and chemical role in forming the active site: A specific lysine residue in the active site must be carbamylated by a CO2 molecule to activate the enzyme. The resulting negatively charged carbamyl group then facilitates the binding of the positively charged Mg2+ion. While other divalent metal ions like Mn2+ can bind to Rubisco, they alter the enzyme's substrate specificity and lead to dramatically lower activity or a higher rate of the non-productive oxygenation reaction compared to Mg2+, making them biologically unfavorable in the context of efficient carbon fixation. The concentration of Mg2+ in the chloroplast stroma naturally increases in the light due to ion potential balancing during ATP synthesis, providing a physiological mechanism to ensure the enzyme is activated when photosynthesis is possible. At the center of the porphyrin ring, nestled within its nitrogen atoms, is a Magnesium ion (Mg2+). This magnesium ion is crucial for the function of chlorophyll, and without it, the pigment cannot effectively capture and transfer light energy. Mg acts as a cofactor: Mg2+ binds to Rubisco after an activator CO2 molecule, forming a catalytically competent complex (Enzyme-CO2-Mg2+). High light + CO2) increases demand: Under high light (60 DLI is a very high intensity, potentially saturating) and high CO2, the plant's capacity for photosynthesis is high, and thus the demand for activated Rubisco and the necessary Mg2+ cofactor increases. Mg deficiency becomes limiting: If Mg2+ is deficient under these conditions, the higher levels of Rubisco and Rubisco activase produced cannot be fully activated, leading to lower photosynthetic rates and potential photo-oxidative damage. Optimal range: Studies show that adequate Mg2+ application can enhance Rubisco activation and stabilize net photosynthetic rates under stress conditions, but the required concentration is specific to the experimental setup. Monitoring is key: The most effective approach in a controlled environment is to monitor the plant's physiological responses e.g., leaf Mg2+ concentration, photosynthetic rate, Rubisco activation state, and adjust the nutrient solution/fertilizer to maintain adequate levels, rather than supplementing a fixed "extra" amount. In practice, this means ensuring that Mg2+ is not a limiting factor in the plant's standard nutrient solution when pushing the limits with high light and CO2. Applying Mg2+ through foliar spray is beneficial to Rubisco regeneration, particularly in alleviating the negative effects of magnesium (Mg) deficiency and high-temperature stress (HTS). While Mg can be leached from soil, within the plant it is considered a mobile nutrient, particularly in the phloem. Foliar-applied Mg is quickly absorbed by the leaves and can be translocate to other plant parts, including new growth and sink organs. Foliar application of: NATURES VERY OWN MgSO4 @ 15.0g L-1 in a spray bottle. For those high-intensity workouts when 1 meal a day is just not enough! Foliar sprays are often recommended as a rapid rescue measure for existing deficiencies or as a supplement during critical growth stages, when demand for Mg is high. Application in the early morning or late evening can improve absorption and prevent leaf burn. The plant was getting a little limey yellow in the centre. Shortly thereafter, she was back in business, green mostly regenerated. The starting point [of creativity] is curiosity: pondering why the default exists in the first place. We’re driven to question defaults when we experience vuja de, the opposite of déjà vu. Déjà vu occurs when we encounter something new, but it feels as if we’ve seen it before. Vuja de is the reverse—we face something familiar, but we see it with a fresh perspective that enables us to gain new insights into old problems. Confidence is evidence... nothing more. You are confident because you have driven 10,000 times, you are confident because you have spoken 10,000 times. People think confidence is a feeling, but it's not. If you want more confidence, then you need to create evidence, take more shots, collect more data, build more experiences, take more risks; fail, confidence doesn't come first; it is the reward you get for doing the work. no one else wants to do.