High, Here is the really last update until you ( and also me) will see it again in ~6-12 days.🤞🏽 At this point I'm pretty happy and really excited. Hopefully around 22° are not too much and it still will taste nice.

Started LSTing. We'll see what a few weeks does to them! Growth is vigorous in DWC. Noticed that the DinaMed's PH was dropping drastically over 24 hours. PPMs were way up at 1100. Diluted the solution by two thirds and re PHed her.

ANTHOCYANIN production is primarily controlled by the Cryptochrome (CR1) Photoreceptor ( !! UV and Blue Spectrums are primary drivers in the production of the pigment that replaces chlorophyll, isn't that awesome! 1. Diverse photoreceptors in plants Many civilizations, including the sun god of ancient Egypt, thought that the blessings of sunlight were the source of life. In fact, the survival of all life, including humans, is supported by the photosynthesis of plants that capture solar energy. Plants that perform photosynthesis have no means of transportation except for some algae. Therefore, it is necessary to monitor various changes in the external environment and respond appropriately to the place to survive. Among various environmental information, light is especially important information for plants that perform photosynthesis. In the process of evolution, plants acquired phytochrome, which mainly receives light in the red light region, and multiple blue light receptors, including his hytropin and phototropin, in order to sense the light environment. .. In addition to these, an ultraviolet light receptor named UVR8 was recently discovered. The latest image of the molecular structure and function of these various plant photoreceptors (Fig. 1), focusing on phytochrome and phototropin. Figure 1 Ultraviolet-visible absorption spectra of phytochrome, cryptochrome, phototropin, and UVR8. The dashed line represents each bioactive absorption spectrum. 2. Phytochrome; red-far red photoreversible molecular switch What is phytochrome? Phytochrome is a photochromic photoreceptor, and has two absorption types, a red light absorption type Pr (absorption maximum wavelength of about 665 nm) and a far-red light absorption type Pfr (730 nm). Reversible light conversion between the two by red light and far-red light, respectively(Fig. 1A, solid line and broken line). In general, Pfr is the active form that causes a physiological response. With some exceptions, phytochrome can be said to function as a photoreversible molecular switch. The background of the discovery is as follows. There are some types of plants that require light for germination (light seed germination). From that study, it was found that germination was induced by red light, the effect was inhibited by subsequent far-red light irradiation, and this could be repeated, and the existence of photoreceptors that reversibly photoconvert was predicted. In 1959, its existence was confirmed by the absorption spectrum measurement of the yellow sprout tissue, and it was named phytochrome. Why does the plant have a sensor to distinguish between such red light and far-red light? There is no big difference between the red and far-red light regions in the open-field spectrum of sunlight, but the proportion of red light is greatly reduced due to the absorption of chloroplasts in the shade of plants. Similar changes in light quality occur in the evening sunlight. Plants perceive this difference in light quality as the ratio of Pr and Pfr, recognize the light environment, and respond to it. Subsequent studies have revealed that it is responsible for various photomorphogenic reactions such as photoperiodic flowering induction, shade repellent, and deyellowing (greening). Furthermore, with the introduction of the model plant Arabidopsis thaliana (At) and the development of molecular biological analysis methods, research has progressed dramatically, and his five types of phytochromes (phyA-E) are present in Arabidopsis thaliana. all right. With the progress of the genome project, Fi’s tochrome-like photoreceptors were found in cyanobacteria, a photosynthetic prokaryotes other than plants. Furthermore, in non-photosynthetic bacteria, a homologue molecule called bacteriophytochrome photoreceptor (BphP) was found in Pseudomonas aeruginosa (Pa) and radiation-resistant bacteria (Deinococcus radiodurans, Dr). Domain structure of phytochrome molecule Phytochrome molecule can be roughly divided into N-terminal side and C-terminal side region. PAS (Per / Arndt / Sim: blue), GAF (cGMP phosphodiesterase / adenylyl cyclase / FhlA: green), PHY (phyto-chrome: purple) 3 in the N-terminal region of plant phytochrome (Fig. 2A) There are two domains and an N-terminal extension region (NTE: dark blue), and phytochromobilin (PΦB), which is one of the ring-opening tetrapyrroles, is thioether-bonded to the system stored in GAF as a chromophore. ing. PAS is a domain involved in the interaction between signal transduction-related proteins, and PHY is a phytochrome-specific domain. There are two PASs and her histidine kinase-related (HKR) domain (red) in the C-terminal region, but the histidine essential for kinase activity is not conserved. 3. Phototropin; photosynthetic efficiency optimized blue light receptor What is phototropin? Charles Darwin, who is famous for his theory of evolution, wrote in his book “The power of move-ment in plants” published in 1882 that plants bend toward blue light. Approximately 100 years later, the protein nph1 (nonphoto-tropic hypocotyl 1) encoded by one of the causative genes of Arabidopsis mutants causing phototropic abnormalities was identified as a blue photoreceptor. Later, another isotype npl1 was found and renamed phototropin 1 (phot1) and 2 (phot2), respectively. In addition to phototropism, phototropin is damaged by chloroplast photolocalization (chloroplasts move through the epidermal cells of the leaves and gather on the cell surface under appropriate light intensity for photosynthesis. As a photoreceptor for reactions such as escaping to the side of cells under dangerous strong light) and stomata (reactions that open stomata to optimize the uptake of carbon dioxide, which is the rate-determining process of photosynthetic reactions). It became clear that it worked. In this way, phototropin can be said to be a blue light receptor responsible for optimizing photosynthetic efficiency. Domain structure and LOV photoreaction of phototropin molecule Phototropin molecule has two photoreceptive domains (LOV1 and LOV2) called LOV (Light-Oxygen-Voltage sensing) on the N-terminal side, and serine / on the C-terminal side. It is a protein kinase that forms threonine kinase (STK) (Fig. 4Aa) and whose activity is regulated by light. LOV is one molecule as a chromophore, he binds FMN (flavin mononucleotide) non-covalently. The LOV forms an α/βfold, and the FMN is located on a β-sheet consisting of five antiparallel β-strands (Fig. 4B). The FMN in the ground state LOV shows the absorption spectrum of a typical oxidized flavin protein with a triplet oscillation structure and an absorption maximum wavelength of 450 nm, and is called D450 (Fig. 1C and Fig. 4E). After being excited to the singlet excited state by blue light, the FMN shifts to the triplet excited state (L660t *) due to intersystem crossing, and then the C4 (Fig. 4C) of the isoaroxazine ring of the FMN is conserved in the vicinity. It forms a transient accretionary prism with the tain (red part in Fig. 4B Eα) (S390I). When this cysteine is replaced with alanine (C / A substitution), the addition reaction does not occur. The effect of adduct formation propagates to the protein moiety, causing kinase activation (S390II). After that, the formed cysteine-flavin adduct spontaneously dissociates and returns to the original D450 (Fig. 4E, dark regression reaction). Phototropin kinase activity control mechanism by LOV2 Why does phototropin have two LOVs? Atphot1 was found as a protein that is rapidly autophosphorylated when irradiated with blue light. The effect of the above C / A substitution on this self-phosphorylation reaction and phototropism was investigated, and LOV2 is the main photomolecular switch in both self-phosphorylation and phototropism. It turns out that it functions as. After that, from experiments using artificial substrates, STK has a constitutive activity, LOV2 functions as an inhibitory domain of this activity, and the inhibition is eliminated by photoreaction, while LOV1 is kinase light. It was shown to modify the photosensitivity of the activation reaction. In addition to this, LOV1 was found to act as a dimerization site from the crystal structure and his SAXS. What kind of molecular mechanism does LOV2 use to photoregulate kinase activity? The following two modules play important roles in this intramolecular signal transduction. Figure 4 (A) Domain structure of LOV photoreceptors. a: Phototropin b: Neochrome c: FKF1 family protein d: Aureochrome (B) Crystal structure of auto barley phot1 LOV2. (C) Structure of FMN isoaroxazine ring. (D) Schematic diagram of the functional domain and module of Arabidopsis thaliana phot1. L, A’α, and Jα represent linker, A’α helix, and Jα helix, respectively. (E) LOV photoreaction. (F) Molecular structure model (mesh) of the LOV2-STK sample (black line) containing A’α of phot2 obtained based on SAXS under dark (top) and under bright (bottom). The yellow, red, and green space-filled models represent the crystal structures of LOV2-Jα, protein kinase A N-lobe, and C-robe, respectively, and black represents FMN. See the text for details. 1) Jα. LOV2 C of oat phot1-to α immediately after the terminus Rix (Jα) is present (Fig. 4D), which interacts with the β-sheet (Fig. 4B) that forms the FMN-bound scaffold of LOV2 in the dark, but unfolds and dissociates from the β-sheet with photoreaction. It was shown by NMR that it does. According to the crystal structure of LOV2-Jα, this Jα is located on the back surface of the β sheet and mainly has a hydrophobic interaction. The formation of S390II causes twisting of the isoaroxazine ring and protonation of N5 (Fig. 4C). As a result, the glutamine side chain present on his Iβ strand (Fig. 4B) in the β-sheet rotates to form a hydrogen bond with this protonated N5. Jα interacts with this his Iβ strand, and these changes are thought to cause the unfold-ing of Jα and dissociation from the β-sheet described above. Experiments such as amino acid substitution of Iβ strands revealed that kinases exhibit constitutive activity when this interaction is eliminated, and that Jα plays an important role in photoactivation of kinases. 2) A’α / Aβ gap. Recently, several results have been reported showing the involvement of amino acids near the A’α helix (Fig. 4D) located upstream of the N-terminal of LOV2 in kinase photoactivation. Therefore, he investigated the role of this A’α and its neighboring amino acids in kinase photoactivation, photoreaction, and Jα structural change for Atphot1. The LOV2-STK polypeptide (Fig. 4D, underlined in black) was used as a photocontrollable kinase for kinase activity analysis. As a result, it was found that the photoactivation of the kinase was abolished when amino acid substitution was introduced into the A’α / Aβ gap between A’α and Aβ of the LOV2 core. Interestingly, he had no effect on the structural changes in Jα examined on the peptide map due to the photoreaction of LOV2 or trypsin degradation. Therefore, the A’α / Aβ gap is considered to play an important role in intramolecular signal transduction after Jα. Structural changes detected by SAXS Structural changes of Jα have been detected by various biophysical methods other than NMR, but structural information on samples including up to STK is reported only by his results to his SAXS. Not. The SAXS measurement of the Atphot2 LOV2-STK polypeptide showed that the radius of inertia increased from 32.4 Å to 34.8 Å, and the molecular model (Fig. 4F) obtained by the ab initio modeling software GASBOR is that of LOV2 and STK. It was shown that the N lobes and C lobes lined up in tandem, and the relative position of LOV2 with respect to STK shifted by about 13 Å under light irradiation. The difference in the molecular model between the two is considered to reflect the structural changes that occur in the Jα and A’α / Aβ gaps mentioned above. Two phototropins with different photosensitivity In the phototropic reaction of Arabidopsis Arabidopsis, Arabidopsis responds to a very wide range of light intensities from 10–4 to 102 μmol photon / sec / m2. At that time, phot1 functions as an optical sensor in a wide range from low light to strong light, while phot2 reacts with light stronger than 1 μmol photon / sec / m2. What is the origin of these differences? As is well known, animal photoreceptors have a high photosensitivity due to the abundance of rhodopsin and the presence of biochemical amplification mechanisms. The exact abundance of phot1 and phot2 in vivo is unknown, but interesting results have been obtained in terms of amplification. The light intensity dependence of the photoactivation of the LOV2-STK polypeptide used in the above kinase analysis was investigated. It was found that phot1 was about 10 times more photosensitive than phot2. On the other hand, when the photochemical reactions of both were examined, it was found that the rate of the dark return reaction of phot1 was about 10 times slower than that of phot2. This result indicates that the longer the lifetime of S390II, which is in the kinase-activated state, the higher the photosensitivity of kinase activation. This correlation was further confirmed by extending the lifespan of her S390II with amino acid substitutions. This alone cannot explain the widespread differences in photosensitivity between phot1 and phot2, but it may explain some of them. Furthermore, it is necessary to investigate in detail protein modifications such as phosphorylation and the effects of phot interacting factors on photosensitivity. Other LOV photoreceptors Among fern plants and green algae, phytochrome ɾphotosensory module (PSM) on the N-terminal side and chimera photoreceptor with full-length phototropin on the C-terminal side, neochrome (Fig. There are types with 4Ab). It has been reported that some neochromes play a role in chloroplast photolocalization as a red light receiver. It is considered that fern plants have such a chimera photoreceptor in order to survive in a habitat such as undergrowth in a jungle where only red light reaches. In addition to this, plants have only one LOV domain, and three proteins involved in the degradation of photomorphogenesis-related proteins, FKF1 (Flavin-binding, Kelch repeat, F-box 1, ZTL (ZEITLUPE)), LKP2 ( There are LOV Kelch Protein2) (Fig. 4Ac) and aureochrome (Fig. 4Ad), which has a bZip domain on the N-terminal side of LOV and functions as a gene transcription factor. 4. Cryptochrome and UVR8 Cryptochrome is one of the blue photoreceptors and forms a superfamily with the DNA photoreceptor photolyase. It has FAD (flavin adenine dinucle-otide) as a chromophore and tetrahydrofolic acid, which is a condensing pigment. The ground state of FAD is considered to be the oxidized type, and the radical type (broken line in Fig. 1B) generated by blue light irradiation is considered to be the signaling state. The radical type also absorbs in the green to orange light region, and may widen the wavelength region of the plant morphogenesis reaction spectrum. Cryptochrome uses blue light to control physiological functions similar to phytochrome. It was identified as a photoreceptor from one of the causative genes of UVR8 Arabidopsis thaliana, and the chromophore is absorbed in the UVB region by a Trp triad consisting of three tryptophans (Fig. 1D). It is involved in the biosynthesis of flavonoids and anthocyanins that function as UV scavengers in plants. Conclusion It is thought that plants have acquired various photoreceptors necessary for their survival during a long evolutionary process. The photoreceptors that cover the existing far-red light to UVB mentioned here are considered to be some of them. More and more diverse photoreceptor genes are conserved in cyanobacteria and marine plankton. By examining these, it is thought that the understanding of plant photoreceptors will be further deepened.

Quest'anno si comincia col botto! Come buon proposito dell'anno nuovo mi sono deciso a metter mano ad un vecchio cassone che c'è in casa mia (prima era una base per un acquario). Ora sarà tut'altro >:D Dopo aver fatto una bella ricerca e raccolta di semi, averli testati e messi alla prova, mi sono finalmente convinto a fare questa benedetta veg box. L'altro box era un 80x80x160, perfetto per la fioritura. Questo invece è un 60x100x80, un box più slim del mio e sdraiato, riesco a dividere lo spazio per tenere madre e cloni contemporaneamente :) Per il momento sto ospitando alcune piante di un amico, piante che dovevano avere dimora in un comodo 120x120 ma che ora sono tutte stipate come sardine a casa mia per le prossime settimane. Mi sto prendendo cura di loro: mi assicuro che germoglino per bene (vedi la girl scout cookies e la runtz in serra), trapianto quando necessario, e mi occupo delle mie bambine personali, la banana sherbet e la critical 707. Piccola curiosità: quando mi ha portato le piantine, stavano tutte a 1 settimana di vita, alcune appena germinate, e ho notato che la bruce banner 3 ha prodotto un gemello! Due germogli da un seme! Sono riuscito a dividerli con successo e mi terrò il gemellino sfortunato per cercare di riportarlo in forze. Al momento ci sono 10 piante in totale: 5 automatiche (tutte del mio amico) e 5(+1) femminizzate (3 mie ,2 del mio amico e 1 in fase di germinazione). Vorrei provare a fare un clone di Monster Skittlez, visto che ha delle foglie particolarmente strane e arricciate (in inglese si dice "webbed" se non sbaglio)... indagherò! Saluti a tutti dal caprone Alpine Goat

Gracias al equipo de Kannabia Seed y XpertNutrients, sin ellos esta magia no seria posible. 🍁💐: Si Runtz se ha ganado su popularidad a pulso, esta versión autofloreciente es digna heredera de sus atributos. No es de extrañar que nuestros criadores hayan acertado con su programa de reproducción, al combinar dos de las cepas más fascinantes de la actualidad: Zkittlez y Gelato. planta resistente y robusta, de cuerpo esbelto y fuerte ramificación lateral, que se puede cultivar prácticamente en cualquier entorno; pero prefiere los veranos largos y cálidos, tal y como quiere la madre naturaleza, donde bañada por el sol se eleva hasta los 120 cm. para producir unos rendimientos muy gratificantes de 100-150 gramos por planta. En interior, y tras solo 70 días desde la semilla, también recompensará con cosechas abundantes de hasta 500 gr./m² Inmediatamente después de encontrarte con ella, lo primero que notarás es su aroma descaradamente afrutado, con un toque tropical cortado con reminiscencias terrosas y amaderadas, que se entremezclan de forma agradable para ofrecer un combo que hace salivar las papilas gustativas. Ofrece un sabor confitado, refrescante y similar a sumergirse en una piscina de frutas cítricas y flores de fragancia intensa. Al inhalar, obtienes la bondad de la dulzura; al exhalar, el humo se convierte en suave y cremoso, dejando suficiente profundidad en cada bocanada para satisfacer a aquellos que buscan los matices más complejos. ⛽ XpertNutrients: es una empresa especializada en la producción y comercialización de fertilizantes líquidos 🍶y sustratos🐛, que garantizan los mejores resultados y cosechas de la más alta calidad. A través de una cuidadosa selección de materias primas y un proceso de producción avanzado, sus productos son sinónimo de resultados confiables. 🛒 Consigue aqui tus fertilizantes: https://xpertnutrients.com/es/sobre-nosotros/#:~:text=Xpert%20Nutrients%20es%20una%20empresa,de%20la%20m%C3%A1s%20alta%20calidad. 📆 Semana 1: Poco que decir, las temperaturas estan siendo algo bajas, se esta desarrollando muy bien hasta ahora, en unos 10 días irá a su lugar definitivo, le estoy ayudando con el TS-3000 de Marshydro para darles un primer empujón, ya que todavía hay pocas horas de luz. Traducir

Heu guys ! Plants start to pre flower, everything is going good, few nutriments fails but overall it's pretty much ok Pretty sad about the Zake and the Shimo in the front that pretty much had no stretch 😓 Cant complain since im working from seeds ! See ya next week ✌️

_____📅 Week 16 | 📅 Day 112 | 🍂 Harvest after the 8. week 🍂 ______ Day 112 🌞🍂🍁💐 - I decided to harvest 2 of the 3 Lemon Power Haze...Exactly after the 8th week. - I'll leave LPH#1 in the tent for at least another week. - Yield is okay...but the buds didn't get even light either...there were too many plants or too long a veggie phase... Day 122 🌞🍂🍁💐 I harvested LPH#1 after 9.5 weeks, I think that did her good Yield dry: LPH#1 - 80 gr LPH#2 - 105gr LPH#3 - 90 gr ____________________ 3 Plants 275 gr total This is now filled into 4 liter preserving jars and stored for at least 1 month with 62% boveda packs. the room temperature is approx. 18-20°. I have not included many small “popcorn buds” as I process them further. It's a big tin I think max 20-30g.

👽

Starting week 7 . Nice purple pistils have shown themselves and the flower are starting to take form. The plants have loved the vodoo juice since the roots have developed more than they used to and the water has never been so clean , and finally the conditions of the growbox are optimal. The plants give a nice citrus and pine smell to the room i am using 80% flowering nutes and removing the b1supermax i am also making sure the plant are going to uptake all the full bloom solution from next week by triggering even more the plant with a. 12/12 light schedule week

Any advice will be greatly appreciated

UPDATE!!! Guys I need some advice, after watering the plants yesterday I noticed these "Rust" marks in the BlackBerry. Are these still Cal/Mag deficiency spots? none of the other plants have them. Hi Growers, I'm surprised how fast the plants have started growing! The BlackBerry by FastBuds has almost doubled in size and has started producing frostly deep purple leaves and smells awesome! Eariler in the week i noticed some Cal/Mag deficiencies so i've added Shogun CalMag to the nutes and it is slowly clearing up the issue. The Zkittles by FastBuds has given me no issues at all this week and has started to smell like fresh raw mangos😍 The two SweetSeeds Honey Peach CBD plants have been awesome too, no issues with nute burn or CalMag. The HP CBD Plant on the back left was planted 1 week after the rest and has somehow grown to the same size as the zkittles and other honey peach. It hasn't started producing any pistills yet so i will be interested to see the yield difference between the two plants.

The stretch conti

Hi! At the end of the second week after switching the light schedule, the plant bloomed. I hope the bush will have time to get the right size. Removed stretch marks, put a metal ring. I wish all kind people a Merry Christmas and a Happy New Year!🎄🎅 Glory to Ukraine! 🇺🇦

Day 80 ~ Plants are looking and smelling great. I have been keeping an eye on the trichomes for the first plant and she looks like she will be ready right after the harvest moon! So I am excited to harvest her on Friday. While plant 2 is still another week and a half to 2 weeks away!

Quarta e ultima semana de Vega assim eu espero, quanto a nutrientes vou continuar com os mesmos, planejo mudar apenas na floração. Iniciei meu treinamento LST com arame e auxílio de palitos para afastar as folhas leque e dar espaço e luz para as gemas. No meio da quarta semana ela entrou na flora Nós vídeos a girl scout Cookies é a planta do fundo

Planta recém transplantada, sendo clones da minha colheita anterior das sementes originais. Foi realizada poda top, além de amarras para moldar o formato das plantas. Também liguei o compressor de ar, já que uso o sistema airpot com o airdome no fundo do vaso para oxigenar as raízes.

Inizio ottava settimana.gia emanano un profumo delizioso .tra una due settimane ci sarà un profumo fortissimo 🤣😂🤤💪buono buono

The flowering started! I'm very excited to see those first little white "hairs" in my plants. Everything is going well, the stems are getting thicker and flowers are groing very fast. I'll keep you all updated with this, send me good vibes!

Fin de las plantas.